Optimization of anode positioning in constructed wetlands coupled with microbial fuel cells based on C/O microenvironment for simultaneous removal of disinfection by-products and nitrogen

Abstract

Constructed wetland coupled with microbial fuel cell (CW-MFC) has been applied for the advanced removal of emerging contaminants and nitrogen due to its sustainability potential. However, the impact of anode positioning on the synergistic removal of disinfection by-products (DBPs) and nitrogen in CW-MFC remains insufficiently understood. In this study, three CW-MFCs with different anode positions were constructed to explore the response differences in the removal of DBPs (represented by haloacetic acids-HAAs) and nitrogen. It was observed that the CW-MFC with the anode positioned centrally exhibited considerable nitrogen removal (TN removal efficiency: 56.3 ± 8.6%) and HAAs degradation performance (dichloroacetic acid removal efficiency: 97.8 ± 1.3%). Correlation analysis identified the advantageous carbon-oxygen environment at the anode as the most critical factor. Furthermore, this carbon-oxygen environment (COD_cr/DO=27.7) directly provided favorable conditions for electroactive bacteria to inhabit the anode, significantly enriching denitrifiers and HAAs-degrading bacteria at the cathode. Key genes (HAAs and carbon-nitrogen metabolic) were upregulated, clarifying the mechanisms of synergistic removal of HAAs and nitrogen in CW-MFCs with centrally positioned anodes. This study highlights the importance of CW-MFCs with anode positioning in the synergistic removal of DBPs and nitrogen, providing straightforward and feasible strategy for optimizing CW-MFC performance and scaling up.