Ultra-Tiny Scale Topographical Cues Direct Arabidopsis Root Growth and Development

Abstract

Plant growth involves intricate processes, including cell division, expansion, and tissue organization, necessitating innovative technologies that emulate native cell–microenvironment interactions. Herein, we introduce ultra-tiny topographical cues (e.g., patterned micro/nanoscale substrates) that mimic micronanofiber structures found in the plant cell wall. We cultured Arabidopsis on unique cell wall-inspired ultra-tiny cues within specialized chambers that positively influenced various physiological aspects compared to a flat surface. Specifically, we observed bidirectional behavior, favoring maximum primary root growth and thickness on sparse features (e.g., 5 μm) and induced predominant anisotropic root alignment on dense features (e.g., 400–800 nm), with alignment decreasing monotonically as the feature size increased. Additionally, RNA sequencing revealed distinct molecular mechanisms underlying Arabidopsis root growth dynamics in response to these ultra-tiny cues, demonstrating modulation of specific genes involved in root development. Collectively, our findings highlight the potential of ultra-tiny cues to modulate gene expression and plant growth dynamics, offering innovative approaches to enhance agricultural productivity sustainably through feature-size-dependent interactions.