Arabidopsis GH3.10 conjugates jasmonates

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2025-03-17 DOI:10.1111/plb.70001
B. Ni, M. Klein, B. Hossbach, K. Feussner, E. Hornung, C. Herrfurth, M. Hamberg, I. Feussner
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拟南芥GH3.10偶联茉莉酸盐。
茉莉酸调节植物的发育和防御。在被子植物中,典型的生物活性茉莉酸是茉莉异亮氨酸(JA-Ile),它在拟南芥中由JAR1和GH3.10形成。然而,与其他茉莉酸生物合成或感知突变体相比,gh3.10 jar1敲除系仍然是可育的。因此,我们进一步研究茉莉酸盐和GH3酶是否有助于调节生育。采用液相色谱-质谱法分析茉莉酸盐水平。利用拟南芥花和叶提取物的非靶向体外代谢组学和体外酶分析,研究了重组GH3.10和相关GH3酶的底物范围。进行茉莉酸盐应用试验,研究其潜在的生物活性。在gh3.10 jar1敲除系的花和伤叶中,JA-Ile均低于检出限。虽然12-羟基- ja被确定为GH3.10的首选底物,但没有其他重组GH3酶能够形成JA-Ile。在没有JA- ile的情况下,在损伤叶片(JA- gln)或花中发现了额外的JA偶联物(JA- gln, JA- asn, JA- glu)。本文介绍了一种新的检测JA-Gln调节生育活性的工具——aos gh3.10 jar1。本研究发现JAR1和GH3.10是拟南芥JA- ile生物合成的唯一参与者,并鉴定了一些JA偶联物作为潜在的生物活性茉莉酸盐,在JA- ile缺失的情况下发挥作用。但是,它们在调节生育率方面的作用还有待最终确定。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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