Immunometabolic effects of β-glucan-trained immunity in newborn goats

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES Research in veterinary science Pub Date : 2025-05-01 Epub Date: 2025-03-16 DOI:10.1016/j.rvsc.2025.105612

Miriam Angulo, Carlos Angulo

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Abstract

Debaryomyces hansenii CBS 8339 β-glucans induced trained immunity in newborn goats. However, the metabolic shifts and potential signaling pathways have not been described yet. Thus, the present study aims to prove, firstly, modifications in cell metabolism related to trained immunity induction (β-glucans) and inhibition (MCC950) in an in vitro model upon lipopolysaccharide (LPS) re-stimulation; secondly, metabolic changes and possible signaling pathways are related to immune memory induced by β-glucan per os in newborns after ex vivo re-stimulation with a bacterial pathogen. Immune training leads to augmenting glycolysis (glucose and lactate) metabolites. Nevertheless, these changes were unaffected by a NOD-like receptor (NLRP3) inhibitor. In vivo training with oral β-glucan doses also evidenced an increase in glycolysis metabolites mediated by up-regulating AKT/MTOR/HIF1Α genes signaling pathway in monocytes; β-glucan in vivo training up-regulated Dectin1, TLR4, TLR6 RAF1, IL1Β and IL6 gene expressions in monocytes, while TNFΑ gene down-regulated. In conclusion, the results demonstrated that D. hansenii β-glucan induced trained immunity in newborn goat monocytes after LPS re-stimulation through glycolysis shifts, which were not reverted by the MCC950 inhibitor.

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β-葡聚糖训练免疫对新生山羊免疫代谢的影响。

汉斯德巴氏菌CBS 8339 β-葡聚糖诱导新生山羊训练免疫。然而，代谢变化和潜在的信号通路尚未被描述。因此，本研究旨在证明，首先，在体外模型中，脂多糖（LPS）再刺激下，与训练免疫诱导（β-葡聚糖）和抑制（MCC950）相关的细胞代谢改变；其次，新生儿体外再刺激细菌病原体后β-葡聚糖诱导的免疫记忆与代谢变化和可能的信号通路有关。免疫训练导致糖酵解（葡萄糖和乳酸）代谢产物增加。然而，这些变化不受nod样受体（NLRP3）抑制剂的影响。口服β-葡聚糖剂量的体内训练也证明糖酵解代谢物的增加是由单核细胞中上调AKT/MTOR/HIF1Α基因信号通路介导的；β-葡聚糖体内训练上调单核细胞中Dectin1、TLR4、TLR6、RAF1、IL1Β和IL6基因表达，下调TNFΑ基因表达。综上所述，结果表明，D. hansenii β-葡聚糖在LPS再刺激后通过糖酵解转移诱导新生山羊单核细胞的训练免疫，而MCC950抑制剂无法逆转这一变化。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.