Synergistic effect of Zinc Solubilizing Bacteria and Consortia on the zinc marker enzymes and gaseous exchange parameters in Rice (Oryza sativa L.) for zinc biofortification

IF 5.7 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2025-03-19 DOI:10.1016/j.plaphy.2025.109807

Tribhuwan Singh , Munmun Kothari , Sudershan Mishra , Ajay veer Singh , A.K. Verma , Deepti Shankhdhar , S.C. Shankhdhar

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Abstract

This study investigates the impact of Zinc Solubilizing Bacteria (ZSB) and their consortia on key physiological and biochemical in two rice varieties, PD 26 and NDR 359, over two consecutive growing seasons (2022 and 2023). Unlike individual bacterial strains, microbial consortia harness the synergistic interactions between multiple species, leading to enhanced zinc solubilization efficiency and improved plant responses. The application of consortia significantly boosted the activities of zinc-dependent enzymes, including carbonic anhydrase (CA) and superoxide dismutase (SOD). It also significantly improved gaseous exchange parameters, including stomatal conductance, internal CO₂ concentration, photosynthetic rate, and transpiration rate. Maximum CA activity in both the varieties was recorded with Consortium1 (T4) and Consortium2 (T5), resulting in an increase of up to 44.60 % and 29.88 % in PD 26 and NDR 359 respectively. Similarly, SOD activity increased by up to 65.11 % in PD 26 and 39.99 % in NDR 359. ZSB consortia treatments also enhanced stomatal conductance by up to 61.92 %, with a significant increase in internal CO₂ concentration, photosynthetic rate, and transpiration rate. Additionally, ZSB positively influenced leaf zinc content and grain yield, with the maximum yield observed with Consortium2 (T5). Regression analysis showed a significant positive correlation between leaf zinc content and CA, SOD activities as well as with photosynthetic rate (R² = 0.64 to 0.95). Principal component analysis (PCA) highlighted the superior performance of consortia treatments in modulating physiological traits, underscoring their potential as sustainable biofortification agents. These findings demonstrate that ZSB consortia outperform individual strains by leveraging multi-strain synergies, leading to enhanced zinc bioavailability and crop productivity.

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增锌菌和菌群对水稻锌标记酶和气体交换参数的协同增效作用及其对锌生物强化的影响

研究了锌溶化菌（ZSB）及其菌群对两个水稻品种PD 26和NDR 359连续两个生长季（2022年和2023年）关键生理生化指标的影响。与单个菌株不同，微生物联合体利用多物种之间的协同相互作用，从而提高锌的溶解效率和改善植物的反应。施用银杏可显著提高锌依赖酶的活性，包括碳酸酐酶（CA）和超氧化物歧化酶（SOD）。它还显著改善了气孔导度、内部CO2浓度、光合速率和蒸腾速率等气体交换参数。两个品种的CA活性均以Consortium1 （T4）和Consortium2 （T5）最高，PD 26和NDR 359的CA活性分别提高了44.60%和29.88%。同样，SOD活性在PD 26和NDR 359中分别增加了65.11%和39.99%。ZSB菌群处理使气孔导度提高了61.92%，显著提高了内部CO2浓度、光合速率和蒸腾速率。此外，ZSB对叶片锌含量和籽粒产量有正向影响，其中以Consortium2 （T5）产量最高。回归分析表明，叶片锌含量与CA、SOD活性、光合速率呈极显著正相关（R2 = 0.64 ~ 0.95）。主成分分析（PCA）强调了财团处理在调节生理性状方面的优越表现，强调了它们作为可持续生物强化剂的潜力。这些发现表明，ZSB群体通过利用多品系协同效应优于单个品系，从而提高锌的生物利用度和作物生产力。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.