{"title":"Efektivitas Biolarvasida Ekstrak Fraksi Polar Daun Citrus hystrix dan Citrus aurantifolia terhadap Culex quinquefasciatus","authors":"Arif Nur Muhammad Ansori, H. Adrianto, H. Hamidah","doi":"10.22435/vektorp.v12i1.261","DOIUrl":null,"url":null,"abstract":"Abstract \nThe development of resistance to chemical insecticides against mosquitoes has beenconsidered as a setback in vector control. This study was aimed to identify LethalConcentration 90% (LC90) of polar fraction extract from Citrus hystrix and Citrusaurantifolia leaves as biolarvicide against mosquito larvae after 24 hours of exposure.This study was an experimental research based on a Completely Randomized Design(CRD). The polar fraction extract of C. hystrix and C. aurantifolia were tested in concentrations of 0 ppm, 500 ppm, 1375 ppm, 2250 ppm, 3125 ppm, and 4000 ppm againstthe 3rd instar larvae of Culex quinquefasciatus. Each treatment was done in five independent replications. The numbers of deceased mosquito larvae were determinedafter 24 hours of treatment and analyzed by probit. The results showed that the polarfraction extract of C. hystrix and C. aurantifolia have bio-larvicidal activity against the 3rdinstar larvae of Cx. quinquefasciatus at LC90 of 1,653 ppm and 2,797 ppm, respectively. In conclusion, the polar fraction extract of C. hystrix leaf is more toxic than C. aurantifolialeaf's against Cx. quinquefasciatus larvae. \nAbstrak \nPerkembangan resistensi terhadap insektisida kimia di antara spesies nyamuk telahdianggap sebagai kemunduran dalam pengendalian vektor. Penelitian ini bertujuanuntuk mengetahui Lethal Concentration 90% (LC90 ) ekstrak fraksi polar daun C. hystrix dan C. aurantifolia sebagai biolarvasida terhadap larva nyamuk setelah paparan 24 jam. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL).Ekstrak fraksi polar daun C. hystrix dan C. aurantifolia diuji dengan konsentrasi 0 ppm,500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm terhadap larva nyamuk Cx.quinquefasciatus instar III. Setiap konsentrasi dilakukan replikasi sebanyak lima kali.Jumlah larva nyamuk yang mati dihitung setelah 24 jam. Setelah itu, data dari larvanyamuk yang mati dianalisis menggunakan probit. Hasil penelitian ini mengungkapkanbahwa ekstrak fraksi polar daun C. hystrix dan C. aurantifolia memiliki aktivitas sebagai biolarvasida terhadap larva nyamuk Cx. quinquefasciatus instar III dengan LC 90= 1.653 ppm dan 2.797 ppm, secara berurutan. Kesimpulan dari penelitian ini adalah ekstrakfraksi polar dari daun C. hystrix lebih toksik dibandingkan dengan C. aurantifoliaterhadap larva nyamuk instar III. \n ","PeriodicalId":55787,"journal":{"name":"Jurnal Vektor Penyakit","volume":"28 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Vektor Penyakit","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22435/vektorp.v12i1.261","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract
The development of resistance to chemical insecticides against mosquitoes has beenconsidered as a setback in vector control. This study was aimed to identify LethalConcentration 90% (LC90) of polar fraction extract from Citrus hystrix and Citrusaurantifolia leaves as biolarvicide against mosquito larvae after 24 hours of exposure.This study was an experimental research based on a Completely Randomized Design(CRD). The polar fraction extract of C. hystrix and C. aurantifolia were tested in concentrations of 0 ppm, 500 ppm, 1375 ppm, 2250 ppm, 3125 ppm, and 4000 ppm againstthe 3rd instar larvae of Culex quinquefasciatus. Each treatment was done in five independent replications. The numbers of deceased mosquito larvae were determinedafter 24 hours of treatment and analyzed by probit. The results showed that the polarfraction extract of C. hystrix and C. aurantifolia have bio-larvicidal activity against the 3rdinstar larvae of Cx. quinquefasciatus at LC90 of 1,653 ppm and 2,797 ppm, respectively. In conclusion, the polar fraction extract of C. hystrix leaf is more toxic than C. aurantifolialeaf's against Cx. quinquefasciatus larvae.
Abstrak
Perkembangan resistensi terhadap insektisida kimia di antara spesies nyamuk telahdianggap sebagai kemunduran dalam pengendalian vektor. Penelitian ini bertujuanuntuk mengetahui Lethal Concentration 90% (LC90 ) ekstrak fraksi polar daun C. hystrix dan C. aurantifolia sebagai biolarvasida terhadap larva nyamuk setelah paparan 24 jam. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL).Ekstrak fraksi polar daun C. hystrix dan C. aurantifolia diuji dengan konsentrasi 0 ppm,500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm terhadap larva nyamuk Cx.quinquefasciatus instar III. Setiap konsentrasi dilakukan replikasi sebanyak lima kali.Jumlah larva nyamuk yang mati dihitung setelah 24 jam. Setelah itu, data dari larvanyamuk yang mati dianalisis menggunakan probit. Hasil penelitian ini mengungkapkanbahwa ekstrak fraksi polar daun C. hystrix dan C. aurantifolia memiliki aktivitas sebagai biolarvasida terhadap larva nyamuk Cx. quinquefasciatus instar III dengan LC 90= 1.653 ppm dan 2.797 ppm, secara berurutan. Kesimpulan dari penelitian ini adalah ekstrakfraksi polar dari daun C. hystrix lebih toksik dibandingkan dengan C. aurantifoliaterhadap larva nyamuk instar III.
蚊虫对化学杀虫剂产生抗药性已成为病媒控制的一大障碍。本研究旨在鉴定柑桔叶和柑橘叶极性部分提取物在24 h后的致死浓度为90% (LC90)。本研究是一项基于完全随机设计(CRD)的实验研究。以0 ppm、500 ppm、1375 ppm、2250 ppm、3125 ppm和4000 ppm的浓度对致倦库蚊3龄幼虫进行了对照试验。每个治疗在5个独立的重复中完成。处理24 h后测定蚊幼虫死亡数,并进行probit分析。结果表明,黄三叶和金三叶的偏析提取物对大蠊3龄幼虫具有一定的生物杀虫活性。致倦库蚊LC90分别为1653 ppm和2797 ppm。综上所述,阴参叶极性部分提取物对Cx的毒性大于金参叶提取物。quinquefasciatus幼虫。【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】【摘要】致死性浓度90% (LC90),极夜蛾蛾和黄叶蛾蛾幼虫,黄叶蛾蛾蛾。Penelitian ini adalah Penelitian实验dengan ranancan Acak Lengkap (RAL)。白桦尺蠖极夜蛾、黄尺蠖、黄尺蠖、白桦尺蠖、白桦尺蠖、白桦尺蠖、白桦尺蠖、白桦尺蠖等。致倦库蚊III。我是说,我是说,我是说,我是说,我是说,我是说,我是说。Jumlah larva nyamuk yang mati dihitung setelah 24 jam。数据分析,数据分析,数据分析,数据分析,数据分析。冬虫夏草、冬虫夏草、冬虫夏草、冬虫夏草、冬虫夏草等。致倦库蚊III型邓根LC 90= 1.653 ppm和2.797 ppm, secara berurutan。3 .大叶蛾蛾幼虫幼虫期3。