In vitro Antioxidant Properties of Mango Powder Produced from Blends of Brokin and Julie Cultivars Fortified with Yellow Pea protein hydrolysate

I. O. Acham, M. Ukeyima, S. Ahemen, A. Girgih, R. Aluko
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Abstract

The aim of the this study was to evaluate the in vitro antioxidant potential of mango powder prepared from blends of Brokin and Julie cultivars fortified with Yellow Pea protein hydrolysates. Six blends were used: *BCI, *JCII, *B80J20, *B70J30, *B60J40 and *B50J50. The fortified blends were derived by obtaining 90% of each of the original unfortified blend and incorporating to each of them, 10% of Yellow pea protein hydrolysate (YPPH). The results of the antioxidant assays were: % inhibition for DPPH radical scavenging activity includes 0.70(*BCI)-5.08%(*B70J30), 2.24(*JCII)-8.69%(*B60J40), 7.79 (*JCII)-12.46% (*BCI), 12.42 (*B60J40)-21.87% (*BCI), 20.60 (*B60J40)-38.57% (*BCI) and 30.08 (*B60J40)-69.64% (*BCI)} at dose concentration levels of 62.50g mL-1, 125.00g mL-1, 250.00µg/mL, 500.00g mL-1, 1000.00g mL-1 and 2000.00g mL-1 respectively; Metal chelating abilities include 51.84 (*B60J40)-66.98 (*B80J20), 64.49 (*JCII)-72.64 (*B50J50), 65.11 (*B60J40)-73.58 (*B80J20), 67.95 (*JCII)-74.59 (*B70J30) and 60.37(*JCII)-75.44% (*B70J30)} at concentrations of 50.00-250.00g mL-1 accordingly; ferric reducing antioxidant power (265.42-411.08µg.AAE mg-1) at those concentration level of 1000.00g mL-1 The % inhibition of hydroxyl radical scavenging activity showed that among all the samples at the concentration range of 62.50-2000.00g mL-1 respectively, samples, *B80J20 and *B50J50 exhibited highest and lowest radical scavenging activities accordingly. Clearly, the best antioxidant activity was observed to be metal chelating ability assay. Based on the aforementioned results, it is concluded that the fortified mango powder samples have good antioxidant activity in vitro which can treat and manage chronic disease condition such as oxidative stress.
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添加黄豌豆蛋白水解物的Brokin和Julie品种混合芒果粉的体外抗氧化性能
以黄豌豆蛋白水解物为强化剂,对Brokin和Julie两种品种的芒果粉进行体外抗氧化活性研究。共混料有*BCI、*JCII、*B80J20、*B70J30、*B60J40和*B50J50。每种原未强化混合物的90%分别加入10%的黄豌豆水解蛋白(YPPH),得到强化混合物。结果表明:在62.50g mL-1、125.00g mL-1、250.00µg/mL、500.00g mL-1、1000.00g mL-1和2000.00g mL-1剂量浓度水平下,对DPPH自由基清除活性的抑制率分别为0.70(* B70J30) -5.08%、2.24(*JCII)-8.69%(*B60J40)、7.79 (*JCII)-12.46% (*BCI)、12.42 (*B60J40)-21.87% (*BCI)、20.60 (*B60J40)-38.57% (*BCI)和30.08 (*B60J40)-69.64% (*BCI)};金属螯合能力分别为51.84 (*B60J40)-66.98 (*B80J20)、64.49 (*JCII)-72.64 (*B50J50)、65.11 (*B60J40)-73.58 (*B80J20)、67.95 (*JCII)-74.59 (*B70J30)和60.37(*JCII)-75.44% (*B70J30)},浓度为50.00-250.00g mL-1;铁还原抗氧化能力(265.42-411.08µg)。对羟基自由基清除活性的%抑制表明,在62.50 ~ 2000.00g mL-1浓度范围内,*B80J20和*B50J50的自由基清除活性分别最高和最低。显然,抗氧化活性最好的是金属螯合能力测定。综上所述,强化芒果粉样品具有良好的体外抗氧化活性,可以治疗和控制氧化应激等慢性疾病。
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