Maria Eugenia Caceres, Andrea Rubini, Luigi Russi, Marilena Ceccarelli, Francesca De Marchis, Michele Bellucci, Fulvio Pupilli
{"title":"在严格裂殖的 Vicia ervilia (L.) Willd.","authors":"Maria Eugenia Caceres, Andrea Rubini, Luigi Russi, Marilena Ceccarelli, Francesca De Marchis, Michele Bellucci, Fulvio Pupilli","doi":"10.1007/s10681-024-03371-w","DOIUrl":null,"url":null,"abstract":"<p>Bitter vetch (<i>Vicia ervilia</i> (L.) Willd.), one of the Near Eastern founder crops, is an annual cleistogamous legume domesticated during the Neolithic period. Originally used for human consumption, over time it was replaced by other pulses and downgraded to a fodder crop. When coupled with a small degree of cross hybridization, cleistogamy confers evolutive plasticity to the plant species. The aim of the present work consisted in setting up optimal conditions to overcome the existing cross hybridization barriers in <i>V. ervilia</i>. Genotypes of Turkish origin, characterized by an erect growth habit were crossed with Italian counterparts characterized by high seed production. A detailed cyto-histological analysis of flower development was undertaken to determine the optimal stage for emasculation and manual cross. Ninety-eight crosses were carried out and the hybrid nature of the putative F1 progenies was assessed by SSR (simple sequence repeat) DNA markers. Fifty-five seeds were obtained of which only five gave rise to hybrid plants. Among these, only three turned out to be fertile and two of which generated a consistent number of F2 seeds whose plants were assessed in greenhouse for seed production and plant growth habit. Most of the evaluated traits showed mean values of the F2 plants intermediate between the two initial parents, confirming that intraspecific hybridization is not only possible but also useful to exploit the diversity confined in different bitter vetch populations.</p>","PeriodicalId":11803,"journal":{"name":"Euphytica","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Obtainment of intraspecific hybrids in strictly cleistogamous Vicia ervilia (L.) Willd.\",\"authors\":\"Maria Eugenia Caceres, Andrea Rubini, Luigi Russi, Marilena Ceccarelli, Francesca De Marchis, Michele Bellucci, Fulvio Pupilli\",\"doi\":\"10.1007/s10681-024-03371-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Bitter vetch (<i>Vicia ervilia</i> (L.) Willd.), one of the Near Eastern founder crops, is an annual cleistogamous legume domesticated during the Neolithic period. Originally used for human consumption, over time it was replaced by other pulses and downgraded to a fodder crop. When coupled with a small degree of cross hybridization, cleistogamy confers evolutive plasticity to the plant species. The aim of the present work consisted in setting up optimal conditions to overcome the existing cross hybridization barriers in <i>V. ervilia</i>. Genotypes of Turkish origin, characterized by an erect growth habit were crossed with Italian counterparts characterized by high seed production. A detailed cyto-histological analysis of flower development was undertaken to determine the optimal stage for emasculation and manual cross. Ninety-eight crosses were carried out and the hybrid nature of the putative F1 progenies was assessed by SSR (simple sequence repeat) DNA markers. Fifty-five seeds were obtained of which only five gave rise to hybrid plants. Among these, only three turned out to be fertile and two of which generated a consistent number of F2 seeds whose plants were assessed in greenhouse for seed production and plant growth habit. Most of the evaluated traits showed mean values of the F2 plants intermediate between the two initial parents, confirming that intraspecific hybridization is not only possible but also useful to exploit the diversity confined in different bitter vetch populations.</p>\",\"PeriodicalId\":11803,\"journal\":{\"name\":\"Euphytica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-06-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Euphytica\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10681-024-03371-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Euphytica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10681-024-03371-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
苦矢车菊(Vicia ervilia (L.) Willd.)是近东的创始作物之一,是一种在新石器时代被驯化的一年生杂交豆科植物。最初用于人类消费,随着时间的推移,它被其他豆类所取代,沦为饲料作物。如果再加上少量的杂交,清花豆科植物就具有了进化的可塑性。本研究的目的是创造最佳条件,以克服 V. ervilia 现有的杂交障碍。以直立生长习性为特征的土耳其原种与以高产种子为特征的意大利原种进行杂交。对花的发育进行了详细的细胞组织学分析,以确定衰退和人工杂交的最佳阶段。共进行了 98 次杂交,并通过 SSR(简单序列重复)DNA 标记评估了推定 F1 后代的杂种性质。共获得 55 颗种子,其中只有 5 颗产生了杂交植株。在这些种子中,只有三粒是可育的,其中两粒产生了数量一致的 F2 种子,在温室中对其植株的种子产量和生长习性进行了评估。大多数被评估的性状显示,F2 植物的平均值介于两个初始亲本之间,这证实了种内杂交不仅是可能的,而且对利用不同苦苣苔种群的多样性很有帮助。
Obtainment of intraspecific hybrids in strictly cleistogamous Vicia ervilia (L.) Willd.
Bitter vetch (Vicia ervilia (L.) Willd.), one of the Near Eastern founder crops, is an annual cleistogamous legume domesticated during the Neolithic period. Originally used for human consumption, over time it was replaced by other pulses and downgraded to a fodder crop. When coupled with a small degree of cross hybridization, cleistogamy confers evolutive plasticity to the plant species. The aim of the present work consisted in setting up optimal conditions to overcome the existing cross hybridization barriers in V. ervilia. Genotypes of Turkish origin, characterized by an erect growth habit were crossed with Italian counterparts characterized by high seed production. A detailed cyto-histological analysis of flower development was undertaken to determine the optimal stage for emasculation and manual cross. Ninety-eight crosses were carried out and the hybrid nature of the putative F1 progenies was assessed by SSR (simple sequence repeat) DNA markers. Fifty-five seeds were obtained of which only five gave rise to hybrid plants. Among these, only three turned out to be fertile and two of which generated a consistent number of F2 seeds whose plants were assessed in greenhouse for seed production and plant growth habit. Most of the evaluated traits showed mean values of the F2 plants intermediate between the two initial parents, confirming that intraspecific hybridization is not only possible but also useful to exploit the diversity confined in different bitter vetch populations.
期刊介绍:
Euphytica is an international journal on theoretical and applied aspects of plant breeding. It publishes critical reviews and papers on the results of original research related to plant breeding.
The integration of modern and traditional plant breeding is a growing field of research using transgenic crop plants and/or marker assisted breeding in combination with traditional breeding tools. The content should cover the interests of researchers directly or indirectly involved in plant breeding, at universities, breeding institutes, seed industries, plant biotech companies and industries using plant raw materials, and promote stability, adaptability and sustainability in agriculture and agro-industries.