胎盘衍生间充质基质样细胞促进三维工程肌肉组织分化和血管网络成熟

IF 11.1 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Small Science Pub Date : 2024-08-06 DOI:10.1002/smsc.202400228
Anna Tsukerman, Majd Machour, Margarita Shuhmaher, Eliana O. Fischer, Hagit Shoyhet, Orit Bar-Am, Gali Guterman Ram, Lior Debbi, Dina Safina, Shulamit Levenberg
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引用次数: 0

摘要

研究表明,胎盘衍生基质样细胞(PLX-PAD)可促进损伤后肌肉组织的恢复并刺激血管生成。这项研究评估了 PLX-PAD 细胞对工程骨骼肌组织血管化和成熟的影响。具体来说,研究人员将其与内皮细胞、周细胞和肌母细胞直接共培养,并将其播种在微孔三维支架中。此外,还监测了低氧 PLX-PAD 细胞条件培养基(CM)对工程组织血管化和肌肉分化的影响。PLX-PAD与肌细胞的共培养刺激了肌细胞分化,而PLX-PAD CM则促进了血管网络的形成。将血管化人骨骼肌组织和 PLX-PAD 的多培养系统植入裸鼠腹直肌缺损处,可促进肌细胞分化、宿主血管穿透和组织整合。这些研究结果表明,胎盘衍生细胞能够诱导血管化工程肌肉构建体的形成,具有潜在的治疗应用价值。
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Placenta-Derived Mesenchymal Stromal-Like Cells Promote 3D-Engineered Muscle Tissue Differentiation and Vessel Network Maturation
Placental-derived stromal-like cells (PLX-PAD) have been shown to facilitate muscle tissue recovery after injury and stimulate angiogenesis. This work assesses the impact of PLX-PAD cells on the vascularization and maturation of engineered skeletal muscle tissue. Specifically, their effects in direct co-culture with endothelial cells, pericytes, and myoblasts seeded within microporous 3D scaffolds are characterized. Additionally, the impact of hypoxic PLX-PAD cell-conditioned medium (CM) on vascularization and muscle differentiation of engineered tissue is monitored. Co-culture of PLX-PAD with myocytes stimulated myocyte differentiation while PLX-PAD CM promoted the formation of vascular networks. Implantation of a multi-culture system of vascularized human skeletal muscle tissue and PLX-PAD into a rectus abdominal defect in nude mice promoted myocyte differentiation, host vessel penetration, and tissue integration. These findings indicate the ability of placenta-derived cells to induce the formation of vascularized engineered muscle constructs with potential therapeutic applications.
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来源期刊
CiteScore
14.00
自引率
2.40%
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期刊介绍: Small Science is a premium multidisciplinary open access journal dedicated to publishing impactful research from all areas of nanoscience and nanotechnology. It features interdisciplinary original research and focused review articles on relevant topics. The journal covers design, characterization, mechanism, technology, and application of micro-/nanoscale structures and systems in various fields including physics, chemistry, materials science, engineering, environmental science, life science, biology, and medicine. It welcomes innovative interdisciplinary research and its readership includes professionals from academia and industry in fields such as chemistry, physics, materials science, biology, engineering, and environmental and analytical science. Small Science is indexed and abstracted in CAS, DOAJ, Clarivate Analytics, ProQuest Central, Publicly Available Content Database, Science Database, SCOPUS, and Web of Science.
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