布氏左旋乳酸杆菌(Levilactobacillus brevis)CD0817 基于混合底物的 pH 自缓冲 GABA 发酵

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-09-13 DOI:10.1007/s00449-024-03088-z
Lingqin Wang, Mengya Jia, Dandan Gao, Haixing Li
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引用次数: 0

摘要

生物活性物质γ-氨基丁酸(GABA)的益生菌发酵是一个极具吸引力的研究课题。已报道的基于单一底物(l-谷氨酸或 l-谷氨酸钠)的 GABA 发酵方法仍有进一步改进的空间。在此,我们设计了一种 pH 自动缓冲策略,以促进 Levilactobacillus brevis CD0817 发酵 GABA。该策略以中性谷氨酸钠和酸性谷氨酸的混合物为底物。这种混合物可提供温和的初始 pH 值;此外,新溶解的 l-谷氨酸可自动抵消底物脱羧引起的 pH 值升高,从而保持 GABA 发酵所必需的酸度。本研究首先进行了烧瓶试验,以优化 Levilactobacillus brevis CD0817 的 GABA 发酵参数。优化后的参数在 10 升发酵罐中进行了进一步验证。烧瓶试验结果表明,合适的发酵培养基由粉末状 l-谷氨酸(750 g/L)、l-谷氨酸钠(34 g/L [0.2 mol/L])、葡萄糖(5 g/L)、酵母提取物(35 g/L)、MnSO4-H2O(50 mg/L [0.3 mmol/L])和吐温 80(1.0 g/L)组成。适宜的发酵温度为 30 °C。发酵罐试验结果表明,GABA 在 0-4 小时内合成缓慢,32 小时内合成迅速,48 小时内达到 353.1 ± 8.3 g/L,pH 值从初始值 4.56 升至最终值 6.10。所提出的 pH 自动缓冲策略可能会在其他 GABA 发酵过程中得到推广。
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Hybrid substrate-based pH autobuffering GABA fermentation by Levilactobacillus brevis CD0817

The probiotic fermentation of the bioactive substance gamma-aminobutyric acid (GABA) is an attractive research topic. There is still room for further improvement in reported GABA fermentation methods based on a single substrate (l-glutamic acid or l-monosodium glutamate). Here, we devised a pH auto-buffering strategy to facilitate the fermentation of GABA by Levilactobacillus brevis CD0817. This strategy features a mixture of neutral monosodium l-glutamate plus acidic l-glutamic acid as the substrate. This mixture provides a mild initial pH; moreover, the newly dissolved l-glutamic acid automatically offsets the pH increase caused by substrate decarboxylation, maintaining the acidity essential for GABA fermentation. In this study, a flask trial was first performed to optimize the GABA fermentation parameters of Levilactobacillus brevis CD0817. The optimized parameters were further validated in a 10 L fermenter. The flask trial results revealed that the appropriate fermentation medium was composed of powdery l-glutamic acid (750 g/L), monosodium l-glutamate (34 g/L [0.2 mol/L]), glucose (5 g/L), yeast extract (35 g/L), MnSO4·H2O (50 mg/L [0.3 mmol/L]), and Tween 80 (1.0 g/L). The appropriate fermentation temperature was 30 °C. The fermenter trial results revealed that GABA was slowly synthesized from 0–4 h, rapidly synthesized until 32 h, and finally reached 353.1 ± 8.3 g/L at 48 h, with the pH increasing from the initial value of 4.56 to the ultimate value of 6.10. The proposed pH auto-buffering strategy may be popular for other GABA fermentations.

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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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