Fei Zheng , HongLuan Li , Jun Yang , Haiyan Wang , Guangle Qin , Dapeng Chen , Jingjie Sha
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Improving macromolecule crowding configurations in nanopores for protein sensing†
We show that PEG-induced macromolecular crowding enhances protein detection in nanopores by increasing capture rate and translocation frequency. Experimental data indicate that a PEG concentration gradient boosts capture efficiency, while our theoretical model attributes this enhancement to osmotic flow, offering insights for improving nanopore-based biosensing.
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