{"title":"柑橘属MPK家族成员的特征及AbMPK13在柑橘溃疡病中的作用分析。","authors":"Lijuan Chen, Minliang Bin, Jue Lin, Wenzhong Shen, Xinxin Zhang","doi":"10.1016/j.plaphy.2024.109418","DOIUrl":null,"url":null,"abstract":"<p><p>Citrus bacterial canker has deleterious effects on global citrus production. The mitogen-activated protein kinase (MAPK) signaling cascade regulates plant defense against pathogen infection. Here, we identified 11 MAPKs in Atalantia buxifolia, a wild citrus species with high stress tolerance. Phylogenetic and gene structure analysis of the identified MAP kinases (MPKs) was conducted, and conserved motifs were identified. MPK expression was altered in Xanthomonas citri subsp. citri (Xcc)-resistant Atalantia buxifolia and Xcc-sensitive sweet orange after Xcc infection. AbMPK13 expression was significantly up-regulated after Xcc infection. AbMPK13 was localized to the cytoplasm, and its overexpression enhanced the resistance of sweet orange to Xcc and activated the salicylic acid (SA) signaling pathway. These findings clarify the role of MPKs in the citrus canker resistance of A. buxifolia.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109418"},"PeriodicalIF":6.1000,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of MPK family members in the genus Citrus (Rutaceae) and analysis of the function of AbMPK13 in the response to citrus canker in Atalantia buxifolia.\",\"authors\":\"Lijuan Chen, Minliang Bin, Jue Lin, Wenzhong Shen, Xinxin Zhang\",\"doi\":\"10.1016/j.plaphy.2024.109418\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Citrus bacterial canker has deleterious effects on global citrus production. The mitogen-activated protein kinase (MAPK) signaling cascade regulates plant defense against pathogen infection. Here, we identified 11 MAPKs in Atalantia buxifolia, a wild citrus species with high stress tolerance. Phylogenetic and gene structure analysis of the identified MAP kinases (MPKs) was conducted, and conserved motifs were identified. MPK expression was altered in Xanthomonas citri subsp. citri (Xcc)-resistant Atalantia buxifolia and Xcc-sensitive sweet orange after Xcc infection. AbMPK13 expression was significantly up-regulated after Xcc infection. AbMPK13 was localized to the cytoplasm, and its overexpression enhanced the resistance of sweet orange to Xcc and activated the salicylic acid (SA) signaling pathway. These findings clarify the role of MPKs in the citrus canker resistance of A. buxifolia.</p>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"219 \",\"pages\":\"109418\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-12-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.plaphy.2024.109418\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.plaphy.2024.109418","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Characterization of MPK family members in the genus Citrus (Rutaceae) and analysis of the function of AbMPK13 in the response to citrus canker in Atalantia buxifolia.
Citrus bacterial canker has deleterious effects on global citrus production. The mitogen-activated protein kinase (MAPK) signaling cascade regulates plant defense against pathogen infection. Here, we identified 11 MAPKs in Atalantia buxifolia, a wild citrus species with high stress tolerance. Phylogenetic and gene structure analysis of the identified MAP kinases (MPKs) was conducted, and conserved motifs were identified. MPK expression was altered in Xanthomonas citri subsp. citri (Xcc)-resistant Atalantia buxifolia and Xcc-sensitive sweet orange after Xcc infection. AbMPK13 expression was significantly up-regulated after Xcc infection. AbMPK13 was localized to the cytoplasm, and its overexpression enhanced the resistance of sweet orange to Xcc and activated the salicylic acid (SA) signaling pathway. These findings clarify the role of MPKs in the citrus canker resistance of A. buxifolia.
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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