Antibiotic resistance profile of facultative deep-sea psychro-piezophile bacteria from the Arabian Sea and their relation with physicochemical factors

Antibiotic resistance (ABR) is a significant global challenge, with antibiotics from various sources ending up in the ocean and affecting marine life. Profiling ABR in deep-sea bacteria is crucial for understanding the spread of ABR from environmental microbes to clinical pathogen and vice-versa. We evaluated facultative psychro-piezophile deep-sea bacteria from different depths of the Arabian Sea for their resistance to 20 commercial antibiotics. Bacteria from Zone 5 (2000–3000 m) exhibited the highest multiple antibiotic resistance (MAR) index (0.90), identifying it as a significant reservoir of ABR. Zone 1 (5–100 m) isolates (average 20 %) showed the highest resistance to synthetic antibiotics. Zone 3 (500–1000 m) isolates were highly resistant to diverse classes of antibiotics, separating upper (zone 1 and 2 (100-500 m) and deeper sea zones (zone 4 (1000–2000 m) and 5). The identified isolates belong to Bacillus, Niallia, Escherichia, Cytobacillus, and Pseudomonas genera. Additionally, antibiotic resistance genes (ARGs) such as StrB (2 isolates) and SXT integrase (1 isolate) were detected only in Zone 5 isolates. The SulII gene (19 isolates) was present across all zones. PCA analysis revealed a negative correlation between resistance and physicochemical factors (macronutrients like phosphate (PO₄³⁻), nitrate (NO₃⁻), nitrite (NO₂⁻), and ammonia (NH₃); micronutrient and heavy metals like (iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), nickel (Ni)), aluminium (Al), cadmium (Cd), and chromium (Cr)), except for Phosphate (0.65). Overall, this study is the first to provide valuable insights into the prevalence of ABR using culture-dependent methods and its correlation with physicochemical factors in the deep-sea environments of the Arabian Sea.