Ju Sung Lee, L. Paje, Joyce P. Rodriguez, K. Kang, D. Hahm, J. Shim, Y. Choi, Sanghyun Lee
{"title":"鸢尾草中鸢尾素HPLC/UV分析方法的验证","authors":"Ju Sung Lee, L. Paje, Joyce P. Rodriguez, K. Kang, D. Hahm, J. Shim, Y. Choi, Sanghyun Lee","doi":"10.22889/KJP.2020.51.3.217","DOIUrl":null,"url":null,"abstract":" Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r 2 0.9999), accuracy (93.9-111.3%), and precision (RSD 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.","PeriodicalId":17851,"journal":{"name":"Korean Journal of Pharmacognosy","volume":"276 1","pages":"217-221"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Validation of an HPLC/UV Analysis Method for Cirsimaritin in Cirsium japonicum var. maackii\",\"authors\":\"Ju Sung Lee, L. Paje, Joyce P. Rodriguez, K. Kang, D. Hahm, J. Shim, Y. Choi, Sanghyun Lee\",\"doi\":\"10.22889/KJP.2020.51.3.217\",\"DOIUrl\":null,\"url\":null,\"abstract\":\" Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r 2 0.9999), accuracy (93.9-111.3%), and precision (RSD 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.\",\"PeriodicalId\":17851,\"journal\":{\"name\":\"Korean Journal of Pharmacognosy\",\"volume\":\"276 1\",\"pages\":\"217-221\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Pharmacognosy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22889/KJP.2020.51.3.217\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Pharmacognosy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22889/KJP.2020.51.3.217","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 2
摘要
猕猴桃(CM)已被用于治疗某些疾病，如出血、肝炎和高血压。西蓟素以前被发现是CM中的主要类黄酮，据说有助于其药理作用。目前还没有详细的报道，定性和定量检测的植物化学指标的空中部分的CM。因此，我们建立了一种高效液相色谱/紫外分光光度法快速鉴定和定量中药中茜草素的方法，并对该方法进行了优化和验证。结果表明:在所检测的浓度范围(0.25 ~ 0.015 mg/mL, r = 2 0.9999)、准确度(93.9 ~ 111.3%)、精密度(RSD0.59%)呈良好的线性关系。因此，该方法可用于中药中茜草素的快速评价。
Validation of an HPLC/UV Analysis Method for Cirsimaritin in Cirsium japonicum var. maackii
Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r 2 0.9999), accuracy (93.9-111.3%), and precision (RSD 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
