Remineralizing capacity of zinc oxide eugenol sealer following the addition of nanohydroxyapatite-tyrosine amino acid: An in vivo animal study.

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2024-10-15 DOI:10.1016/j.job.2024.09.006
Rasha M Al-Shamaa, Raghad A Al-Askary
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Abstract

Objective: Although several sealers have been developed, the zinc oxide eugenol (ZOE) sealer is still used in many private dental clinics. This study aimed to compare the biocompatibility and remineralizing capacity οf ZOE sealer with the addition of nanohydroxyapatite-tyrosine amino acid.

Methods: This study was conducted on Twenty rabbits. The rabbits were divided into four groups based on the test observation period (3, 7, 21, and 28 days) following surgical implantation. General anesthesia was administered to each rabbit, and a subcutaneous incision of approximately 1±0.5 cm was made along the symphyseal area of the mandible of each rabbit. Four bone cavities were generated in the interdental space of the lower jaw between the central and molar teeth of each rabbit, with one longitudinal subcutaneous incision. The ZOE sealers were mixed according to the manufacturer's guidelines and directly inserted within the cavities generated at the end of each test period. The animals were sacrificed, and bone biopsy was carried out at the site of testing. The biopsy samples were obtained and subjected to histological analysis using a low-power light microscope (Olympus C ⅹ 21, Japan) and immunohistochemistry using Ki67 antibody.

Results: The collected information was examined by parametric statistical tests using SPSS software version ''22." One-way ANOVA and post-hoc Duncan's tests were used to measure the significance among various groups, with statistical significance set, when "P≤0.01".

Conclusion: The 20% mixed endodontic sealer displayed excellent outcomes compared to other experimental groups, as identified by higher new bone formation at every evaluation period.

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添加纳米羟基磷灰石-酪氨酸氨基酸后氧化锌丁香酚封闭剂的再矿化能力:活体动物研究。
目的:尽管已开发出多种封闭剂,但许多私人牙科诊所仍在使用氧化锌丁香酚(ZOE)封闭剂。本研究旨在比较添加纳米羟基磷灰石-酪氨酸氨基酸的氧化锌封闭剂的生物相容性和再矿化能力:本研究以 20 只兔子为对象。根据手术植入后的试验观察期(3、7、21 和 28 天)将兔子分为四组。对每只兔子进行全身麻醉,沿每只兔子下颌骨的骨骺区做一个约 1±0.5 厘米的皮下切口。在每只兔子下颌中牙和磨牙之间的牙间隙生成四个骨腔,其中一个为纵向皮下切口。按照制造商的指导原则混合 ZOE 密封剂,并在每个测试期结束时直接将其插入生成的骨腔中。动物被处死,并在测试部位进行骨活检。获得活检样本后,使用低倍光学显微镜(日本奥林巴斯 C ⅹ 21)进行组织学分析,并使用 Ki67 抗体进行免疫组化:使用 SPSS 软件版本''22 "对收集的信息进行参数统计检验。采用单因素方差分析和事后邓肯检验来衡量各组间的显著性,当 "P≤0.01 "时,统计学意义成立:结论:与其他实验组相比,20% 混合根管封闭剂的效果非常好,在每个评估阶段都有较高的新骨形成。
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来源期刊
Journal of Oral Biosciences
Journal of Oral Biosciences DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
4.40
自引率
12.50%
发文量
57
审稿时长
37 days
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