Targeted metabolomics analysis of fatty acids in lamb meat for the authentication of paper mulberry silage as a substitute for alfalfa silage

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Chemical and Biological Technologies in Agriculture Pub Date : 2024-10-29 DOI:10.1186/s40538-024-00688-5

Xiong Yi, Zhou Hongzhang, Wang Ruhui, Li Xiaomei, Lin Yanli, Shi Yue, Ni Kuikui, Yang Fuyu

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Abstract

Background

The paper mulberry (Broussonetia papyrifera L.) is a valuable source of woody forage that can be used for ruminants, such as goat and lambs. However, there is limited knowledge about how paper mulberry silage affects carcass characteristics, meat physicochemical attributes, amino acid and unsaturated fatty acids in Hu lambs compared to alfalfa silage. The objective of this experiment was to investigate the impact of substituting alfalfa silage with paper mulberry silage on the slaughter performance, meat quality, free amino acid and fatty acid composition in muscles of Hu lambs.

Results

Thirty 60-day-old male Hu lambs with 17.13 ± 0.26 kg body weight were randomly allocated into paper mulberry silage (30% of total mixed ration) and alfalfa silage (30% of total mixed ration) treatment, and the feeding trial lasted 70 days. The results indicated no significant differences in all measurements (P > 0.05). However, compared to the group fed with alfalfa silage, the group fed with paper mulberry silage exhibited a reduction in meat drip loss (P < 0.05) without any notable effect on meat nutrients (P > 0.05). Targeted metabolomics analysis revealed that feeding paper mulberry silage led to decreased levels of certain bitter amino acids, such as valine, leucine, isoleucine, tryptophan, and phenylalanine (P < 0.05). Furthermore, the consumption of paper mulberry silage significantly augmented the levels of monounsaturated and polyunsaturated fatty acids, particularly n6-polyunsaturated fatty acids (C18:2n6, C20:3n6, C20:4n6, etc.), in meat.

Conclusions

Substituting paper mulberry silage for alfalfa in the daily diet of Hu lambs not only has no detrimental effect on animal performance but also improves meat unsaturated fatty acid composition.

Graphical Abstract

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对羔羊肉中的脂肪酸进行靶向代谢组学分析，以鉴定纸桑青贮饲料是否可替代苜蓿青贮饲料

背景纸桑树（Broussonetia papyrifera L.）是一种宝贵的木本饲料来源，可用于饲喂山羊和羔羊等反刍动物。然而，与紫花苜蓿青贮饲料相比，人们对纸桑青贮饲料如何影响胡羔羊的胴体特征、肉的理化属性、氨基酸和不饱和脂肪酸的了解还很有限。本试验的目的是研究用纸桑青贮替代苜蓿青贮对胡羊羔屠宰性能、肉质、肌肉中游离氨基酸和脂肪酸组成的影响。结果将 30 只体重为 17.13 ± 0.26 kg 的 60 日龄雄性胡羊羔随机分配到纸桑青贮（占混合日粮总量的 30%）和苜蓿青贮（占混合日粮总量的 30%）处理中，饲养试验持续 70 天。结果表明，所有测定指标均无显著差异（P > 0.05）。然而，与饲喂苜蓿青贮饲料组相比，饲喂纸桑青贮饲料组的肉滴损失减少（P <0.05），但对肉的营养成分没有明显影响（P >0.05）。靶向代谢组学分析表明，饲喂纸桑椹青贮饲料会导致某些苦味氨基酸水平下降，如缬氨酸、亮氨酸、异亮氨酸、色氨酸和苯丙氨酸（P <0.05）。结论在胡羔羊的日粮中用纸质桑青贮代替苜蓿不仅不会对动物的生产性能产生不利影响，而且还能改善肉中不饱和脂肪酸的组成。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.