tRNA regulation and amino acid usage bias reflect a coordinated metabolic adaptation in Plasmodium falciparum

IF 4.6 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-10-12 DOI:10.1016/j.isci.2024.111167
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Abstract

An adaptive feature of malaria-causing parasites is the digestion of host hemoglobin (HB) to acquire amino acids (AAs). Here, we describe a link between nutrient availability and translation dependent regulation of gene expression as an adaptive strategy. We show that tRNA expression in Plasmodium falciparum does not match the decoding need expected for optimal translation. A subset of tRNAs decoding AAs that are insufficiently provided by HB are lowly expressed, wherein the abundance of a protein-coding transcript is negatively correlated with the decoding requirement of these tRNAs. Proliferation-related genes have evolved a high requirement of these tRNAs, thereby proliferation can be modulated by repressing protein synthesis of these genes during nutrient stress. We conclude that the parasite modulates translation elongation by maintaining a discordant tRNA profile to exploit variations in AA-composition among genes as an adaptation strategy. This study exemplifies metabolic adaptation as an important driving force for protein evolution.

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tRNA调控和氨基酸使用偏差反映了恶性疟原虫协调的代谢适应性
疟疾致病寄生虫的一个适应性特征是消化宿主血红蛋白(HB)以获取氨基酸(AA)。在这里,我们描述了营养物质的可用性与基因表达的翻译依赖性调控之间的联系,这是一种适应性策略。我们发现,恶性疟原虫的 tRNA 表达与最佳翻译所需的解码需求并不匹配。对 HB 提供不足的 AA 进行解码的 tRNA 子集表达量很低,其中蛋白质编码转录本的丰度与这些 tRNA 的解码需求呈负相关。与增殖相关的基因对这些 tRNA 的需求量很高,因此在营养胁迫期间可以通过抑制这些基因的蛋白质合成来调节增殖。我们的结论是,寄生虫通过维持不和谐的 tRNA 配置文件来调节翻译伸长,从而利用基因间 AA 组成的变化作为一种适应策略。这项研究证明,代谢适应是蛋白质进化的重要驱动力。
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来源期刊
iScience
iScience Multidisciplinary-Multidisciplinary
CiteScore
7.20
自引率
1.70%
发文量
1972
审稿时长
6 weeks
期刊介绍: Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.
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