Overcoming off-target optical stimulation-evoked cortical activity in the mouse brain in vivo

IF 4.6 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-10-15 DOI:10.1016/j.isci.2024.111152
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Abstract

Exogenous opsins allow for in vivo interrogation of brain circuits at unprecedented temporal and spatial precision. Here, we found that optical fiber laser stimulation at wavelengths of 637, 594, or 473 nm within the cortex of mice lacking expression of exogenous opsins resulted in a strong neuronal response in the contralateral visual cortex. Evoked responses were observed even at low laser intensities (fiber tip power 1 mW) and most pronounced at 637 nm. We took advantage of retinal light adaptation by using a dim external light source (20 lux) that abolished the 594 and 473 nm-evoked neuronal responses even at high laser intensities (15 mW). The prevention of 637 nm-evoked responses, however, could only be achieved for stimulation intensities 2.5 mW. This highlights the need for careful selection of light wavelengths and intensities for optogenetic experiments. Additionally, retinal light adaptation offers an effective solution to minimize unintended activation.

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克服小鼠体内光学刺激诱发皮层活动的脱靶问题
外源性视蛋白能以前所未有的时间和空间精度在体内检测大脑回路。在这里,我们发现光纤激光刺激波长为 637、594 或 473 nm 的缺乏外源视蛋白表达的小鼠大脑皮层会导致对侧视觉皮层出现强烈的神经元反应。即使在激光强度较低(光纤尖端功率为 1 mW)的情况下,也能观察到诱发反应,其中 637 nm 波段的反应最为明显。我们利用视网膜的光适应性,使用昏暗的外部光源(20 勒克斯),即使在高激光强度(15 毫瓦)下也能消除 594 和 473 纳米波长诱发的神经元反应。然而,只有在刺激强度≤ 2.5 mW时才能阻止 637 nm诱发的反应。这凸显了在光遗传学实验中仔细选择光波长和强度的必要性。此外,视网膜光适应提供了一种有效的解决方案,可最大限度地减少意外激活。
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来源期刊
iScience
iScience Multidisciplinary-Multidisciplinary
CiteScore
7.20
自引率
1.70%
发文量
1972
审稿时长
6 weeks
期刊介绍: Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.
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