Effect of electron beam irradiation on the pine wood nematode, Bursaphelenchus xylophilus.

IF 2.1 4区医学 Q2 BIOLOGY International Journal of Radiation Biology Pub Date : 2023-01-01 Epub Date: 2023-07-12 DOI:10.1080/09553002.2023.2232035

Junheon Kim, Sujin Lee, Hae-Jun Park

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引用次数: 1

Abstract

Purpose: Reproduction inhibition of the pine wood nematode (PWN) by electron beam (e-beam) irradiation both in vitro and in vivo was tested to determine if ionizing radiation could control the PWN by reducing survival and preventing reproduction, thus reducing the risk of pine wilt disease (PWD) spread.

Materials and methods: E-beam (10 MeV) irradiation treatment at different doses (0-4 kGy) was applied to PWNs in a Petri dish. Treatment of pine wood logs infested with PWNs was performed at 10 kGy. Mortality was determined by comparing the survival rates before and after irradiation treatment. DNA damage by e-beam irradiation (0-10 kGy) in the PWN was determined using the comet assay.

Results: E-beam irradiation increased mortality and suppressed reproduction with increasing doses. The lethal dose (LD) values (kGy) were estimated as follows: LD₅₀ = 2.32, LD₉₀ = 5.03, and LD₉₉ = 9.48. E-beam irradiation of pine wood logs significantly suppressed PWN reproduction. Comets of e-beam-irradiated cells showed an increased tail DNA level and moment with an increasing dose.

Conclusion: This study suggests that e-beam irradiation could be used as an alternative method for the management of pine wood logs infested with PWNs.

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电子束辐照对松材线虫的影响。

目的:研究电子束辐照对松材线虫(PWN)体外和体内繁殖的抑制作用，以确定电离辐射是否能通过降低松材线虫的存活和抑制其繁殖来控制PWN，从而降低松材线虫病(PWD)的传播风险。材料和方法:采用不同剂量(0-4 kGy)的电子束(10 MeV)辐照处理培养皿中的PWNs。对侵染PWNs的松木原木进行了10 kGy的处理。通过比较放射治疗前后的存活率来确定死亡率。采用彗星法测定电子束辐照(0-10 kGy)对PWN的DNA损伤。结果:随着剂量的增加，电子束辐照增加了死亡率，抑制了繁殖。致死剂量(LD)值(kGy)估计如下:LD50 = 2.32, LD90 = 5.03, LD99 = 9.48。松木原木的电子束辐照显著抑制了PWN的繁殖。随着剂量的增加，辐射细胞的彗尾DNA水平和瞬间增加。结论:电子束辐照可作为一种管理PWNs侵染松材原木的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Radiation Biology 医学-核科学技术

CiteScore

5.00

自引率

11.50%

发文量

142

审稿时长

3 months

期刊介绍： The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.