Pub Date : 2025-02-13DOI: 10.1007/s00114-025-01968-2
Sylvain Dubey, Sébastien Pellaud, Samuel Furrer, Christophe Dufresnes
The frog leg industry relies on a global, largely underregulated market with potentially important ecological impact such as the uncontrolled harvest of declining wild populations and the introduction of invasive species. Here, we inferred the taxonomic nature and geographic origins of frog legs imported to Switzerland by DNA barcoding. Out of 34 samples, we retrieved eight distinct lineages attributed to five species from four genera, namely Hoplobatrachus rugulosus from Vietnam, Fejervarya cancrivora from Indonesia (invasive on several Pacific islands), two phylogeographic lineages of Limnonectes macrodon from Western and Central Java, L. kadarsani from eastern Indonesia, and three phylogeographic lineages of Pelophylax ridibundus from northern and central southern Turkey (invasive in Western Europe). Only the first two species were correctly declared, which is particularly problematic to track down harvests of the declining and geographically restricted Limnonectes taxa. In this respect, we show that the three Asian genera can be reliably distinguished by basic measurements of the frog legs, which could be used in future forensic controls. Our study calls for more stringent international regulations of the frog trade, including shipment monitoring to document the relative abundance of harvested species and ensure the sustainability of their wild populations.
{"title":"Unsuspected diversity and multiple origins of the frog legs imported to Switzerland for human consumption, as determined by DNA barcoding and morphology","authors":"Sylvain Dubey, Sébastien Pellaud, Samuel Furrer, Christophe Dufresnes","doi":"10.1007/s00114-025-01968-2","DOIUrl":"10.1007/s00114-025-01968-2","url":null,"abstract":"<div><p>The frog leg industry relies on a global, largely underregulated market with potentially important ecological impact such as the uncontrolled harvest of declining wild populations and the introduction of invasive species. Here, we inferred the taxonomic nature and geographic origins of frog legs imported to Switzerland by DNA barcoding. Out of 34 samples, we retrieved eight distinct lineages attributed to five species from four genera, namely <i>Hoplobatrachus rugulosus</i> from Vietnam, <i>Fejervarya cancrivora</i> from Indonesia (invasive on several Pacific islands), two phylogeographic lineages of <i>Limnonectes macrodon</i> from Western and Central Java, <i>L. kadarsani</i> from eastern Indonesia, and three phylogeographic lineages of <i>Pelophylax ridibundus</i> from northern and central southern Turkey (invasive in Western Europe). Only the first two species were correctly declared, which is particularly problematic to track down harvests of the declining and geographically restricted <i>Limnonectes</i> taxa. In this respect, we show that the three Asian genera can be reliably distinguished by basic measurements of the frog legs, which could be used in future forensic controls. Our study calls for more stringent international regulations of the frog trade, including shipment monitoring to document the relative abundance of harvested species and ensure the sustainability of their wild populations.</p></div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"112 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-025-01968-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhongmiao Wang, Jie Hou, Jiang Xu, Kun Yang, Daohui Lin
Microorganisms are pivotal in decomposing persistent organic pollutants in the environment. However, the bioavailability of pollutants is often hindered by the strong adsorption to ubiquitous fine colloids. This study provides the first evidence that Rhodococcus biphenylivorans, a known intracellular oxidative degradation bacterium, can reductively degrade organochlorine pollutants adsorbed on biochar colloids (BCCs) extracellularly, besides the oxidative degradation of BCCs-adsorbed pollutants intracellularly. Over a 30-day biodegradation period, the total biodegradation rates of adsorbed 2,4,4'-trichlorobiphenyl (PCB28, 10 mg/L) on BCCs (1 g/L) prepared at pyrolysis temperatures of 300, 500, and 700 ℃ were 59.4%, 34.8%, and 10.4%, respectively. The biodegradation products of adsorbed PCB28 on low-temperature BCCs were mainly chlorosubstituted-2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid-type products, while in the high-temperature BCCs system, dichlorobiphenyl was the main product. Mechanistically, BCCs with the low pyrolysis temperature could fragment during the degradation, facilitating the transport of adsorbed PCB28 into bacteria for the intracellular oxidative degradation; the interactions between bacterial cells and BCCs (especially of high pyrolysis temperature) could stimulate the respiratory chain electron efflux for the extracellular dechlorination degradation. These findings not only provide new insights into the modulatory influence of BCCs on microbial degradation of pollutants, but also enrich our knowledge on the environmental fate of colloids-adsorbed pollutants.
{"title":"Biochar colloids act as both transporters of organic pollutants and stimulants of respiratory chain electron efflux: a new understanding of microbial degradation of adsorbed pollutants","authors":"Zhongmiao Wang, Jie Hou, Jiang Xu, Kun Yang, Daohui Lin","doi":"10.1039/d4en01019a","DOIUrl":"https://doi.org/10.1039/d4en01019a","url":null,"abstract":"Microorganisms are pivotal in decomposing persistent organic pollutants in the environment. However, the bioavailability of pollutants is often hindered by the strong adsorption to ubiquitous fine colloids. This study provides the first evidence that Rhodococcus biphenylivorans, a known intracellular oxidative degradation bacterium, can reductively degrade organochlorine pollutants adsorbed on biochar colloids (BCCs) extracellularly, besides the oxidative degradation of BCCs-adsorbed pollutants intracellularly. Over a 30-day biodegradation period, the total biodegradation rates of adsorbed 2,4,4'-trichlorobiphenyl (PCB28, 10 mg/L) on BCCs (1 g/L) prepared at pyrolysis temperatures of 300, 500, and 700 ℃ were 59.4%, 34.8%, and 10.4%, respectively. The biodegradation products of adsorbed PCB28 on low-temperature BCCs were mainly chlorosubstituted-2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid-type products, while in the high-temperature BCCs system, dichlorobiphenyl was the main product. Mechanistically, BCCs with the low pyrolysis temperature could fragment during the degradation, facilitating the transport of adsorbed PCB28 into bacteria for the intracellular oxidative degradation; the interactions between bacterial cells and BCCs (especially of high pyrolysis temperature) could stimulate the respiratory chain electron efflux for the extracellular dechlorination degradation. These findings not only provide new insights into the modulatory influence of BCCs on microbial degradation of pollutants, but also enrich our knowledge on the environmental fate of colloids-adsorbed pollutants.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"21 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haipeng Zhang, Jie Chen, Xinyue Liu, Rui Wang, Hongcheng Zhang, Yanju Yang
ZZinc (Zn) is a critical co-factor for enzymes involved in photosynthesis, delaying leaf senescence and enhancing photosynthetic efficiency. Supplementing rice leaves with Zn can improve yield, quality, and Zn content in edible parts, addressing food security and micronutrient deficiencies. In this study, we evaluated the effects of spraying Zn oxide nanoparticles (ZnO NPs) (0, 5, 10, and 20 mg L-1) at the rice panicle initiation stage on photosynthesis, yield, and grain quality through a two-year field experiment. Results showed foliar application of ZnO NPs at the panicle initiation stage increased leaf area index, net photosynthetic rate, and photosynthetic potential, leading to a 1.5%-6.4% increase in grain yield through higher grain filling rate and 1000-grain weight. ZnO NPs also delayed leaf senescence and prolonged the duration of active photosynthesis, which significantly contributed to higher biomass production and improved grain filling, further enhancing yield. Additionally, the enhancement in photosynthetic efficiency and delayed senescence promoted the production of high-quality grains. ZnO NPs improved rice appearance quality by reducing the chalkiness grain rate and degree. Rice tasting value increased by 3.3%-7.0%, reflecting improvements in appearance, viscosity, and balance, along with reductions in hardness. ZnO NPs raised peak viscosity and breakdown values while lowering setback values. Furthermore, ZnO NPs significantly increased Zn content in brown and milled rice by 13.8%-56.0% and 20.1%-78.6%, respectively, and improved Zn bioavailability by reducing the phytate-to-zinc molar ratio. These findings highlight the potential of ZnO NPs as a sustainable nanotechnology-based approach to simultaneously improve rice productivity, quality, and nutritional value, offering a promising solution for addressing food security and micronutrient deficiency in rice-based diets.
{"title":"Enhancing rice (Oryza sativa L.) yield and quality by improving photosynthesis with zinc oxide nanoparticles foliar application","authors":"Haipeng Zhang, Jie Chen, Xinyue Liu, Rui Wang, Hongcheng Zhang, Yanju Yang","doi":"10.1039/d4en01209g","DOIUrl":"https://doi.org/10.1039/d4en01209g","url":null,"abstract":"ZZinc (Zn) is a critical co-factor for enzymes involved in photosynthesis, delaying leaf senescence and enhancing photosynthetic efficiency. Supplementing rice leaves with Zn can improve yield, quality, and Zn content in edible parts, addressing food security and micronutrient deficiencies. In this study, we evaluated the effects of spraying Zn oxide nanoparticles (ZnO NPs) (0, 5, 10, and 20 mg L-1) at the rice panicle initiation stage on photosynthesis, yield, and grain quality through a two-year field experiment. Results showed foliar application of ZnO NPs at the panicle initiation stage increased leaf area index, net photosynthetic rate, and photosynthetic potential, leading to a 1.5%-6.4% increase in grain yield through higher grain filling rate and 1000-grain weight. ZnO NPs also delayed leaf senescence and prolonged the duration of active photosynthesis, which significantly contributed to higher biomass production and improved grain filling, further enhancing yield. Additionally, the enhancement in photosynthetic efficiency and delayed senescence promoted the production of high-quality grains. ZnO NPs improved rice appearance quality by reducing the chalkiness grain rate and degree. Rice tasting value increased by 3.3%-7.0%, reflecting improvements in appearance, viscosity, and balance, along with reductions in hardness. ZnO NPs raised peak viscosity and breakdown values while lowering setback values. Furthermore, ZnO NPs significantly increased Zn content in brown and milled rice by 13.8%-56.0% and 20.1%-78.6%, respectively, and improved Zn bioavailability by reducing the phytate-to-zinc molar ratio. These findings highlight the potential of ZnO NPs as a sustainable nanotechnology-based approach to simultaneously improve rice productivity, quality, and nutritional value, offering a promising solution for addressing food security and micronutrient deficiency in rice-based diets.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"55 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11DOI: 10.1007/s11270-025-07787-5
Irina V. Minenkova, Aleksey V. Ul’yanov, Svetlana V. Popova, Aleksey A. Sobolev, Ivan S. Pytskii, Aleksey K. Buryak
A method for preparing a peat-shungite sorbent with the addition of a potassium-phosphate buffer solution for neutralizing hydrazine derivatives is proposed. The peat-shungite sorbent is characterized by ease of manufacture, relative cheapness, in addition, the main components are of natural origin. Sorbents with different shungite contents (25, 34, 42 and 50 wt.%) were prepared. With a peat/shungite ratio of 65/35 wt.%, the sorbent is the most resistant to mechanical destruction. According to the proposed method, the sorbent was prepared in different forms: powder, granules, cubes. The efficiency of the peat-shungite sorbent was tested in the process of neutralizing highly toxic 1,1-dimethylhydrazine and some products of its transformation. It was found that with an initial concentration of the ecotoxicant of 500 g/kg, the maximum permissible concentration is reached on the 60th day of interaction. The studies carried out and the calculated kinetic parameters of the neutralization process showed that shungite-containing sorbents are approximately 1.6 times more effective than peat.
{"title":"Method of preparation and application of peat-shungite sorbent for detoxification of hydrazine derivatives","authors":"Irina V. Minenkova, Aleksey V. Ul’yanov, Svetlana V. Popova, Aleksey A. Sobolev, Ivan S. Pytskii, Aleksey K. Buryak","doi":"10.1007/s11270-025-07787-5","DOIUrl":"10.1007/s11270-025-07787-5","url":null,"abstract":"<div><p>A method for preparing a peat-shungite sorbent with the addition of a potassium-phosphate buffer solution for neutralizing hydrazine derivatives is proposed. The peat-shungite sorbent is characterized by ease of manufacture, relative cheapness, in addition, the main components are of natural origin. Sorbents with different shungite contents (25, 34, 42 and 50 wt.%) were prepared. With a peat/shungite ratio of 65/35 wt.%, the sorbent is the most resistant to mechanical destruction. According to the proposed method, the sorbent was prepared in different forms: powder, granules, cubes. The efficiency of the peat-shungite sorbent was tested in the process of neutralizing highly toxic 1,1-dimethylhydrazine and some products of its transformation. It was found that with an initial concentration of the ecotoxicant of 500 g/kg, the maximum permissible concentration is reached on the 60th day of interaction. The studies carried out and the calculated kinetic parameters of the neutralization process showed that shungite-containing sorbents are approximately 1.6 times more effective than peat.</p></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study introduces M_CNF/biochar, a novel plant growth stimulant comprising the bamboo-derived biochar, boron (B), molybdenum (Mo)-, and copper (Cu)-carbon nanofibers (CNF). The prepared micro-nano formulation is successfully used to deliver the B-Mo-Cu multi micronutrients to the Cicer arietinum (chickpea) plant, with the CNF acting as a translocator for the micronutrients. The synthesized material is thoroughly characterized for its physicochemical property using various analytical techniques. The results show that a M_CNF/biochar-dose of 1 g kg⁻¹ soil significantly enhances plant growth, indicated by an increase in the fresh biomass, root and shoot lengths, and the protein and chlorophyll contents. Furthermore, the soil's water-holding capacity increases by more than 90% with the mixing of M_CNF/biochar. The results also reveal that the total nitrogen content of the soil amended with M_CNF/biochar increases more than 4 times, post-30 days of the plant growth, indicating an improvement in the nitrogen fixation capacity of the rhizosphere. This study has successfully presented the bamboo-derived biochar modified with micronutrients for sustainable agriculture.
{"title":"A Micro-Nano Formulation of Multi-Micronutrients- and Carbon Nanofiber-Modified Biochar for Enhanced Plant Growth","authors":"Abhishek Gupta, Rahul Gupta, Nishith Verma","doi":"10.1039/d4en00802b","DOIUrl":"https://doi.org/10.1039/d4en00802b","url":null,"abstract":"This study introduces M_CNF/biochar, a novel plant growth stimulant comprising the bamboo-derived biochar, boron (B), molybdenum (Mo)-, and copper (Cu)-carbon nanofibers (CNF). The prepared micro-nano formulation is successfully used to deliver the B-Mo-Cu multi micronutrients to the Cicer arietinum (chickpea) plant, with the CNF acting as a translocator for the micronutrients. The synthesized material is thoroughly characterized for its physicochemical property using various analytical techniques. The results show that a M_CNF/biochar-dose of 1 g kg⁻¹ soil significantly enhances plant growth, indicated by an increase in the fresh biomass, root and shoot lengths, and the protein and chlorophyll contents. Furthermore, the soil's water-holding capacity increases by more than 90% with the mixing of M_CNF/biochar. The results also reveal that the total nitrogen content of the soil amended with M_CNF/biochar increases more than 4 times, post-30 days of the plant growth, indicating an improvement in the nitrogen fixation capacity of the rhizosphere. This study has successfully presented the bamboo-derived biochar modified with micronutrients for sustainable agriculture.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"2 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1007/s00114-025-01962-8
Russell D. C. Bicknell, Adiël A. Klompmaker, Patrick M. Smith, Thomas A. Hegna
Marine isopod fossils represent a small component of the crustacean fossil record, contrasting the exceptional modern diversity of marine representatives of Isopoda. Examination of previously documented isopod species therefore presents an opportunity to derive additional paleobiological and taphonomic insight of these rare fossils. Here we consider two clusters of Archaeoniscus brodiei from the Lower Cretaceous (middle to upper Berriasian) Intermarine Member of the Durlston Formation, England. The individuals within the clusters are mostly complete, of similar size ranges, and are preserved on two different bedding planes. After examining these individuals, we illustrate, for the first time, appendages and eyes of A. brodiei. The appendage morphology supports the interpretation of A. brodiei as an isopod adapted to a benthic lifestyle. We propose that isopods from the Durlston Formation follow similar taphonomic pathways to arthropods preserved within plattenkalk-like deposits, resulting in enrichment in calcium carbonate and phosphate. Finally, the clusters reflect gregarious activities that were preserved during hypoxic events brought on by concurrent decay of algal blooms.
{"title":"Exploring the morphology and taphonomy of Archaeoniscus brodiei—a gregarious, Early Cretaceous isopod","authors":"Russell D. C. Bicknell, Adiël A. Klompmaker, Patrick M. Smith, Thomas A. Hegna","doi":"10.1007/s00114-025-01962-8","DOIUrl":"10.1007/s00114-025-01962-8","url":null,"abstract":"<div><p>Marine isopod fossils represent a small component of the crustacean fossil record, contrasting the exceptional modern diversity of marine representatives of Isopoda. Examination of previously documented isopod species therefore presents an opportunity to derive additional paleobiological and taphonomic insight of these rare fossils. Here we consider two clusters of <i>Archaeoniscus brodiei</i> from the Lower Cretaceous (middle to upper Berriasian) Intermarine Member of the Durlston Formation, England. The individuals within the clusters are mostly complete, of similar size ranges, and are preserved on two different bedding planes. After examining these individuals, we illustrate, for the first time, appendages and eyes of <i>A. brodiei</i>. The appendage morphology supports the interpretation of <i>A. brodiei</i> as an isopod adapted to a benthic lifestyle. We propose that isopods from the Durlston Formation follow similar taphonomic pathways to arthropods preserved within plattenkalk-like deposits, resulting in enrichment in calcium carbonate and phosphate. Finally, the clusters reflect gregarious activities that were preserved during hypoxic events brought on by concurrent decay of algal blooms.</p></div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"112 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-025-01962-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The dyeing industry wastewater has greatly hampered the ecological environment. The carbon quantum dots (CQDs) is a promising catalyst for photocatalytic degradation. To control the specified structure of CQDs, a designable-green deep eutectic solvents (DESs) was targeted as the precursor. In this work, a copper-doped solid-state Cu-CQDs was successfully prepared by combustion method from glycerol/choline chloride/CuCl2·2H2O DESs, and characterized by Fourier transform infrared spectroscopy, XRD, XPS, UV–Vis spectroscopy, and fluorescence spectroscopy. The analysis revealed that the metallic copper-doped Cu-CQDs have better morphological and structural properties and exhibit good optics characteristics. The prepared CQDs were applied to the photocatalytic degradation of Rhodamine-B (RhB). It was found that the ⋅O2− was the main active specie, and it can efficient degrade RhB up to 95%.