Pub Date : 2026-06-15Epub Date: 2026-02-11DOI: 10.1016/j.bios.2026.118517
Tiger Haoran Shi , John Alex Sinclair , Youwen Zhang , Xuemin Lu , Sonu Kumar , Xin Lu , Satyajyoti Senapati , Hsueh-Chia Chang
Metalloproteinases (MPs) such as a-disintegrin and metalloproteinase-10 (ADAM-10) are key drivers of extracellular matrix remodeling during tumor progression, yet MP-based liquid biopsy tests have not reached clinical utility. Here, we show that active ADAM-10 is selectively enriched on the surface of circulating extracellular vesicles (EVs) in the plasma of colorectal cancer patients. Our findings further suggest ADAM-10+ EVs are locally enriched in dense pre-metastatic tumor extracellular matrices and subsequently accumulate in blood post-metastasis. To capture these unique signatures of disease progression, an ADAM-10 activity assay is integrated with a novel size-sensitive Immuno-Janus Particle affinity assay for characterizing ADAM-10+ EVs in untreated plasma. In a 43-patient colorectal cancer cohort, this multimodal platform distinguishes healthy, pre-metastatic, and metastatic states with 95% overall accuracy. When combined with lipidomics as a third modality, the platform correctly determines 97.4% cancer stage accuracy, with only one misclassification. This study establishes a multimodal EV-based activity/affinity assay as a robust framework for liquid biopsy, providing accurate cancer staging, improved prognostics, and offering a potential platform for pan-disease diagnostics.
{"title":"Multimodal activity-affinity assay of ADAM-10 extracellular vesicles in untreated plasma reveals metastatic stage of colorectal cancer","authors":"Tiger Haoran Shi , John Alex Sinclair , Youwen Zhang , Xuemin Lu , Sonu Kumar , Xin Lu , Satyajyoti Senapati , Hsueh-Chia Chang","doi":"10.1016/j.bios.2026.118517","DOIUrl":"10.1016/j.bios.2026.118517","url":null,"abstract":"<div><div>Metalloproteinases (MPs) such as a-disintegrin and metalloproteinase-10 (ADAM-10) are key drivers of extracellular matrix remodeling during tumor progression, yet MP-based liquid biopsy tests have not reached clinical utility. Here, we show that active ADAM-10 is selectively enriched on the surface of circulating extracellular vesicles (EVs) in the plasma of colorectal cancer patients. Our findings further suggest ADAM-10+ EVs are locally enriched in dense pre-metastatic tumor extracellular matrices and subsequently accumulate in blood post-metastasis. To capture these unique signatures of disease progression, an ADAM-10 activity assay is integrated with a novel size-sensitive Immuno-Janus Particle affinity assay for characterizing ADAM-10+ EVs in untreated plasma. In a 43-patient colorectal cancer cohort, this multimodal platform distinguishes healthy, pre-metastatic, and metastatic states with 95% overall accuracy. When combined with lipidomics as a third modality, the platform correctly determines 97.4% cancer stage accuracy, with only one misclassification. This study establishes a multimodal EV-based activity/affinity assay as a robust framework for liquid biopsy, providing accurate cancer staging, improved prognostics, and offering a potential platform for pan-disease diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"302 ","pages":"Article 118517"},"PeriodicalIF":10.5,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146196854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-01-28DOI: 10.1016/j.colsurfb.2026.115473
Yuzheng Zhou , Chunli Ma , Yibin Sun , Yuan Wu , Tongfeng Han , Yongqiang Xue , Guotai Li , Qihui Zhou , Zhaodong Du
Rhegmatogenous retinal detachment (RRD) is a severe eye condition that can threaten vision without proper treatment. The primary treatment for RRD is vitrectomy, however, the postoperative requirement for a prone position, coupled with unsatisfactory retinal reattachment rates, presents a significant clinical challenge. Recent advancements in technology and material engineering have led to the development of specific biomaterials serving as retinal patches that can seal retinal breaks. Whereas, existing materials still face limitations including the need for specialized implantation instruments and complex surgical operations for certain materials, inadequate conformability to the eyeball due to relatively high material rigidity that impairs sealing performance, and poor biocompatibility. To overcome these difficulties, herein we introduce a novel fluorescent hydrogel composed of 4-arm-PEG-Mal and 4-arm-PEG-SH as a retinal sealant. This innovative material undergoes rapid gelation in response to the weakly alkaline intraocular environment following injury, enabling effective sealing of retinal breaks and the surrounding area. Furthermore, the hydrogel's inherent fluorescent property provides enhanced intraoperative visibility. Evaluated in a rabbit model of retinal detachment, the hydrogel demonstrated efficient therapeutic treatment in promoting RRD repair, while offering significant visual and surgical advantages. In conclusion, the retinal break-sealing hydrogel developed in this work presents a promising new strategy for the treatment of RRD, with considerable potential for clinical translation.
{"title":"Intraocular-microenvironment responsive fluorescent hydrogels in rhegmatogenous retinal detachment repair","authors":"Yuzheng Zhou , Chunli Ma , Yibin Sun , Yuan Wu , Tongfeng Han , Yongqiang Xue , Guotai Li , Qihui Zhou , Zhaodong Du","doi":"10.1016/j.colsurfb.2026.115473","DOIUrl":"10.1016/j.colsurfb.2026.115473","url":null,"abstract":"<div><div>Rhegmatogenous retinal detachment (RRD) is a severe eye condition that can threaten vision without proper treatment. The primary treatment for RRD is vitrectomy, however, the postoperative requirement for a prone position, coupled with unsatisfactory retinal reattachment rates, presents a significant clinical challenge. Recent advancements in technology and material engineering have led to the development of specific biomaterials serving as retinal patches that can seal retinal breaks. Whereas, existing materials still face limitations including the need for specialized implantation instruments and complex surgical operations for certain materials, inadequate conformability to the eyeball due to relatively high material rigidity that impairs sealing performance, and poor biocompatibility. To overcome these difficulties, herein we introduce a novel fluorescent hydrogel composed of 4-arm-PEG-Mal and 4-arm-PEG-SH as a retinal sealant. This innovative material undergoes rapid gelation in response to the weakly alkaline intraocular environment following injury, enabling effective sealing of retinal breaks and the surrounding area. Furthermore, the hydrogel's inherent fluorescent property provides enhanced intraoperative visibility. Evaluated in a rabbit model of retinal detachment, the hydrogel demonstrated efficient therapeutic treatment in promoting RRD repair, while offering significant visual and surgical advantages. In conclusion, the retinal break-sealing hydrogel developed in this work presents a promising new strategy for the treatment of RRD, with considerable potential for clinical translation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"262 ","pages":"Article 115473"},"PeriodicalIF":5.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-01-29DOI: 10.1016/j.bios.2026.118461
Farhan N. Rahman , Prabhkirat S. Bindra , Afra Nawar , H. Trask Crane , Jesus Antonio Sanchez-Perez , John A. Berkebile , Onur Selim Kilic , Chuoqi Chen , Vikram Abbaraju , Christopher J. Nichols , Jacob M. Cook , Samer Mabrouk , Asim H. Gazi , Jin-Oh Hahn , Omer T. Inan
<div><h3>Background</h3><div>Acute stress, in moderation, helps to prepare the body to overcome mental and physical challenges. However, excessive bouts of acute stress can be detrimental to the cardiovascular system and are a risk factor for cardiovascular disease and sudden cardiac death. Transcutaneous median nerve stimulation (tMNS) is a promising therapy for the mitigation of acute stress through peripheral neuromodulation, but the optimal delivery of tMNS for stress mitigation may require continuous monitoring of acute stress events for targeted delivery.</div></div><div><h3>Objective</h3><div>The purpose of this study was to develop a wearable system capable of continuous closed-loop acute stress monitoring and mitigation through non-invasive cardiovascular sensing and tMNS respectively.</div></div><div><h3>Methods</h3><div>A wearable wrist-worn device capable of sensing three channels of photoplethysmogram (PPG) and tri-axial accelerometry was designed. Pulse rate (PR) and PPG amplitude (PPGamp) were extracted from the acquired green PPG signal, while tMNS was delivered at varying intensities by custom-designed analog circuitry onboard the device. A companion app was used to wirelessly set stimulation levels by communicating with the device's microcontroller using Bluetooth low energy. The device was validated against bench-top sensors in a study with 19 healthy participants involving acute mental and physical stressors as well as tMNS. Repeated-measures correlation and Bland-Altman analyses were performed to compare PR extracted from 9904 5-s windows of PPG from the device and heart rate (HR) extracted beat-by-beat from bench-top electrocardiogram (ECG) and averaged across the same windows. Statistical tests were also performed to analyze differences in mean PR and PPGamp from baseline metrics across the acute stress and tMNS protocol.</div></div><div><h3>Results</h3><div>PR extracted from our device correlated (r = 0.871, p < 0.001) and agreed (mean difference: 0.51 bpm, 95 % limits of agreement: 6.58 bpm, 5.57 bpm) strongly with HR extracted from bench-top ECG. We found decreases in mean PPGamp from baseline during stressors, while application of tMNS alongside stressors increased PPGamp back to baseline levels, and continued delivery of tMNS post-stressor further increased PPGamp to a significant difference from baseline. Significant reductions in PR as compared to baseline post-physical stressor also mirrored these findings, suggesting that our wearable device can track elevations in acute stress through cardiovascular monitoring while also mitigating the effects of acute stress through tMNS.</div></div><div><h3>Conclusion</h3><div>Our device is the first wearable, to our knowledge, to enable continuous monitoring of acute stress through cardiovascular sensing and feature extraction while mitigating acute stress through peripheral neuromodulation. Future work should test the device in ambulatory settings and investigate potential
{"title":"A wearable system enabling acute stress monitoring and closed-loop mitigation through transcutaneous median nerve stimulation","authors":"Farhan N. Rahman , Prabhkirat S. Bindra , Afra Nawar , H. Trask Crane , Jesus Antonio Sanchez-Perez , John A. Berkebile , Onur Selim Kilic , Chuoqi Chen , Vikram Abbaraju , Christopher J. Nichols , Jacob M. Cook , Samer Mabrouk , Asim H. Gazi , Jin-Oh Hahn , Omer T. Inan","doi":"10.1016/j.bios.2026.118461","DOIUrl":"10.1016/j.bios.2026.118461","url":null,"abstract":"<div><h3>Background</h3><div>Acute stress, in moderation, helps to prepare the body to overcome mental and physical challenges. However, excessive bouts of acute stress can be detrimental to the cardiovascular system and are a risk factor for cardiovascular disease and sudden cardiac death. Transcutaneous median nerve stimulation (tMNS) is a promising therapy for the mitigation of acute stress through peripheral neuromodulation, but the optimal delivery of tMNS for stress mitigation may require continuous monitoring of acute stress events for targeted delivery.</div></div><div><h3>Objective</h3><div>The purpose of this study was to develop a wearable system capable of continuous closed-loop acute stress monitoring and mitigation through non-invasive cardiovascular sensing and tMNS respectively.</div></div><div><h3>Methods</h3><div>A wearable wrist-worn device capable of sensing three channels of photoplethysmogram (PPG) and tri-axial accelerometry was designed. Pulse rate (PR) and PPG amplitude (PPGamp) were extracted from the acquired green PPG signal, while tMNS was delivered at varying intensities by custom-designed analog circuitry onboard the device. A companion app was used to wirelessly set stimulation levels by communicating with the device's microcontroller using Bluetooth low energy. The device was validated against bench-top sensors in a study with 19 healthy participants involving acute mental and physical stressors as well as tMNS. Repeated-measures correlation and Bland-Altman analyses were performed to compare PR extracted from 9904 5-s windows of PPG from the device and heart rate (HR) extracted beat-by-beat from bench-top electrocardiogram (ECG) and averaged across the same windows. Statistical tests were also performed to analyze differences in mean PR and PPGamp from baseline metrics across the acute stress and tMNS protocol.</div></div><div><h3>Results</h3><div>PR extracted from our device correlated (r = 0.871, p < 0.001) and agreed (mean difference: 0.51 bpm, 95 % limits of agreement: 6.58 bpm, 5.57 bpm) strongly with HR extracted from bench-top ECG. We found decreases in mean PPGamp from baseline during stressors, while application of tMNS alongside stressors increased PPGamp back to baseline levels, and continued delivery of tMNS post-stressor further increased PPGamp to a significant difference from baseline. Significant reductions in PR as compared to baseline post-physical stressor also mirrored these findings, suggesting that our wearable device can track elevations in acute stress through cardiovascular monitoring while also mitigating the effects of acute stress through tMNS.</div></div><div><h3>Conclusion</h3><div>Our device is the first wearable, to our knowledge, to enable continuous monitoring of acute stress through cardiovascular sensing and feature extraction while mitigating acute stress through peripheral neuromodulation. Future work should test the device in ambulatory settings and investigate potential ","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118461"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-02-06DOI: 10.1016/j.bios.2026.118484
Yang Li , Haozhen Ren , Runze Wu , Jiahui Wang , Yuanxun Gong , Qianli Tang , Xianjiu Liao , Kai Zhang , Longjian Huang , Jihua Wei
B-type natriuretic peptide (BNP) is an important biomarker for cardiovascular diseases, motivating sensitive and robust quantification in complex matrices. Here we report a single-electrode, anti-correlated dual-mode electrochemiluminescence/surface-enhanced Raman scattering (ECL/SERS) biosensor that couples split-aptamer recognition with a solution-phase T7 transcription–CRISPR/Cas13a cascade and an interfacial toehold-mediated strand-displacement “probe-stripping” transduction on a CsPbBr3@PDA@Au-modified glassy carbon electrode. BNP binding releases an active T7 template to generate trigger RNA, which activates Cas13a collateral cleavage to produce initiator strands for interfacial unlocking. The interface reaction removes ferrocene/Raman co-labeled probes, synchronizing ECL turn-on with SERS turn-off and enabling ratiometric quantification (R = IECL/ISERS) to suppress common-mode variability. The sensor provides BNP determination over 0–106 aM with log-linear single-mode calibrations and a continuous ratiometric response. Selectivity was validated against multiple interferents and the structurally related peptide NT-proBNP at the same concentration (106 aM), showing negligible ratiometric change relative to BNP. Serum-sample evaluation and stability tests further support feasibility in complex matrices. This work establishes a cascade-to-interface ratiometric strategy for robust protein biosensing.
{"title":"A dual-mode electrochemiluminescence/SERS biosensor for B-type natriuretic peptide based on a T7–CRISPR/Cas13a cascade and a CsPbBr3@PDA@Au perovskite interface","authors":"Yang Li , Haozhen Ren , Runze Wu , Jiahui Wang , Yuanxun Gong , Qianli Tang , Xianjiu Liao , Kai Zhang , Longjian Huang , Jihua Wei","doi":"10.1016/j.bios.2026.118484","DOIUrl":"10.1016/j.bios.2026.118484","url":null,"abstract":"<div><div>B-type natriuretic peptide (BNP) is an important biomarker for cardiovascular diseases, motivating sensitive and robust quantification in complex matrices. Here we report a single-electrode, anti-correlated dual-mode electrochemiluminescence/surface-enhanced Raman scattering (ECL/SERS) biosensor that couples split-aptamer recognition with a solution-phase T7 transcription–CRISPR/Cas13a cascade and an interfacial toehold-mediated strand-displacement “probe-stripping” transduction on a CsPbBr<sub>3</sub>@PDA@Au-modified glassy carbon electrode. BNP binding releases an active T7 template to generate trigger RNA, which activates Cas13a collateral cleavage to produce initiator strands for interfacial unlocking. The interface reaction removes ferrocene/Raman co-labeled probes, synchronizing ECL turn-on with SERS turn-off and enabling ratiometric quantification (R = I<sub>ECL</sub>/I<sub>SERS</sub>) to suppress common-mode variability. The sensor provides BNP determination over 0–10<sup>6</sup> aM with log-linear single-mode calibrations and a continuous ratiometric response. Selectivity was validated against multiple interferents and the structurally related peptide NT-proBNP at the same concentration (10<sup>6</sup> aM), showing negligible ratiometric change relative to BNP. Serum-sample evaluation and stability tests further support feasibility in complex matrices. This work establishes a cascade-to-interface ratiometric strategy for robust protein biosensing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118484"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anisotropic gold nanoparticles with surface plasmon resonance (SPR) can generate heat upon exposure to laser light, which can then be utilised for photothermal therapy (PTT) in cancer treatment. In this study, we report the use of doxorubicin (DOX)-loaded immunomodulatory polysaccharide (PST001) coated anisotropic gold nanostars (AuNS@PST) as a theranostic carrier for photothermal-chemotherapy. AuNS@PST were prepared by green synthesis followed by doxorubicin encapsulation. The synthesized particles were characterized using UV–vis spectroscopy, DLS, FTIR and TEM. The heat dissipation of these nanoparticles was monitored in aqueous phantoms using 635 nm laser sources, which indicated the thermal rise from ambient temperature. The in vitro cytotoxicity analysis of AuNS@PST was done using the MTT assay in A549 cells. Significantly lower IC50 value was observed for cells treated with DOX-loaded PST AuNSs when compared to DOX-alone treated cells. Similarly, DOX-loaded AuNS@PST had efficient photothermal-induced apoptosis exerted by the laser-irradiated nanoparticles. Changes in protein degradation and DNA fragmentation at the subcellular levels were observed in the Raman spectrum. Although supplementary perspectives are required in the proper investigation of laser-mediated cell death in cancer tissues, the current study discloses the emerging methodology to track apoptotic events in cancer tissues using the Raman scattering platform, even at the sub-cellular level.
{"title":"Exploration of sub-cellular responses for the evaluation of the laser mediated tumor ablation via Raman spectroscopic platform using green synthesized gold nanostars","authors":"BS Unnikrishnan , GU Preethi , PT Sujai , Kaustabh Kumar Maiti , TT Sreelekha","doi":"10.1016/j.colsurfb.2026.115472","DOIUrl":"10.1016/j.colsurfb.2026.115472","url":null,"abstract":"<div><div>Anisotropic gold nanoparticles with surface plasmon resonance (SPR) can generate heat upon exposure to laser light, which can then be utilised for photothermal therapy (PTT) in cancer treatment. In this study, we report the use of doxorubicin (DOX)-loaded immunomodulatory polysaccharide (PST001) coated anisotropic gold nanostars (AuNS@PST) as a theranostic carrier for photothermal-chemotherapy. AuNS@PST were prepared by green synthesis followed by doxorubicin encapsulation. The synthesized particles were characterized using UV–vis spectroscopy, DLS, FTIR and TEM. The heat dissipation of these nanoparticles was monitored in aqueous phantoms using 635 nm laser sources, which indicated the thermal rise from ambient temperature. The <em>in vitro</em> cytotoxicity analysis of AuNS@PST was done using the MTT assay in A549 cells. Significantly lower IC<sub>50</sub> value was observed for cells treated with DOX-loaded PST AuNSs when compared to DOX-alone treated cells. Similarly, DOX-loaded AuNS@PST had efficient photothermal-induced apoptosis exerted by the laser-irradiated nanoparticles. Changes in protein degradation and DNA fragmentation at the subcellular levels were observed in the Raman spectrum. Although supplementary perspectives are required in the proper investigation of laser-mediated cell death in cancer tissues, the current study discloses the emerging methodology to track apoptotic events in cancer tissues using the Raman scattering platform, even at the sub-cellular level.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"262 ","pages":"Article 115472"},"PeriodicalIF":5.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-01-27DOI: 10.1016/j.colsurfb.2026.115475
Hao Gong , Zhengfeng Lu , Suming Wei , Yongjun Rui
Osteosarcoma (OS)-induced bone abnormalities present a considerable clinical challenge due to elevated chances of recurrence and compromised repair, which significantly jeopardize patient survival. Contemporary bioactive glasses (BGs), notwithstanding their osteogenic potential, exhibit restricted anticancer efficacy. Therefore, It is essential to enhance the tumor-killing efficacy of BGs for usage as a filler in tumor-induced bone defects. Here, a copper (Cu)-doped 13–93BG (13–93BG-Cu) was synthesized and subsequently combined with chitosan to form the 13–93BG-Cu system. The rapid release of Cu ions (Cu2 +) during the initial stages of this system enhances the killing of tumor cells by cuproptosis, as intracellular Cu2+ accumulation triggers the oxidative stress response within mitochondria, hence achieving anti-OS therapy. Subsequently, the sustained low-level release of Cu2+ and bioactive ions collaboratively influences the activation and function of macrophage and stem cells, promoting bone defect healing. This study introduces a dual-action BG that simultaneously neutralizes the acidic tumor microenvironment and promotes cuproptosis, effectively preventing recurrence while facilitating bone healing via Cu2+ gradient release.
{"title":"Copper-infused 13–93 bioactive glass inhibiting postoperative osteosarcoma recurrence and enhancing bone regeneration","authors":"Hao Gong , Zhengfeng Lu , Suming Wei , Yongjun Rui","doi":"10.1016/j.colsurfb.2026.115475","DOIUrl":"10.1016/j.colsurfb.2026.115475","url":null,"abstract":"<div><div>Osteosarcoma (OS)-induced bone abnormalities present a considerable clinical challenge due to elevated chances of recurrence and compromised repair, which significantly jeopardize patient survival. Contemporary bioactive glasses (BGs), notwithstanding their osteogenic potential, exhibit restricted anticancer efficacy. Therefore, It is essential to enhance the tumor-killing efficacy of BGs for usage as a filler in tumor-induced bone defects. Here, a copper (Cu)-doped 13–93BG (13–93BG-Cu) was synthesized and subsequently combined with chitosan to form the 13–93BG-Cu system. The rapid release of Cu ions (Cu<sup>2 +</sup>) during the initial stages of this system enhances the killing of tumor cells by cuproptosis, as intracellular Cu<sup>2+</sup> accumulation triggers the oxidative stress response within mitochondria, hence achieving anti-OS therapy. Subsequently, the sustained low-level release of Cu<sup>2+</sup> and bioactive ions collaboratively influences the activation and function of macrophage and stem cells, promoting bone defect healing. This study introduces a dual-action BG that simultaneously neutralizes the acidic tumor microenvironment and promotes cuproptosis, effectively preventing recurrence while facilitating bone healing via Cu<sup>2+</sup> gradient release.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"262 ","pages":"Article 115475"},"PeriodicalIF":5.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-01-23DOI: 10.1016/j.colsurfb.2026.115464
Fengmei Yang , Yutong Chen , Yujiao Yan , Ruixin Zhao , Liran Deng , Die Tian , Meng Xie
Pathological events in Alzheimer’s disease (AD) typically involve β-amyloid (Aβ) plaque deposition, metal ion dysregulation, oxidative stress elevation, and chronic neuroinflammation, making single-target therapies unsatisfactory. Here, we first report a biomimetic nanoplatform based on red blood cell membrane–coated cerium metal–organic frameworks (Ce-MOF-RBC) that enables multi-target synergistic intervention against AD. The Ce-MOF core exhibits potent antioxidant activity, efficiently scavenging reactive oxygen species (ROS) and restoring mitochondrial membrane potential, while its carboxylate ligands chelate Cu2 + with high efficiency (49.26 %) to inhibit Cu2+-induced Aβ fibrillation and disassemble preformed fibrils. Ce-MOF-RBC further modulates microglial phenotype, enhancing Aβ phagocytosis and reducing neuroinflammation. Importantly, RBC membrane functionalization markedly improves biological performance by prolonging systemic circulation, enhancing blood–brain barrier (BBB) penetration, and leveraging its intrinsic affinity for Aβ peptides to enrich Aβ. In vivo fluorescence imaging and brain cryosections showed that Ce-MOF-RBC achieved robust accumulation in the cortex and hippocampus, with brain fluorescence intensities 27.33-fold higher than free DiD. In the C. elegans AD model, Ce-MOF-RBC reduced Aβ plaque fluorescence by 32.54 %, lowered ROS levels by 45.72 %, improved chemotaxis performance (chemotaxis index increased from 34.24 % to 68.34 %), and delayed paralysis onset from 10 h to 15 h, demonstrating significant rescue of cognitive and motor deficits. In summary, these findings highlight the first demonstration of a small-sized, biomimetic Ce-MOF-RBC nanoplatform that integrates antioxidant, metal-chelating, anti-aggregation, and immunomodulatory functions, offering a promising strategy for comprehensive AD therapy.
{"title":"Biomimetic red blood cell membrane–coated cerium metal–organic framework for multi-target synergistic therapy of Alzheimer’s disease","authors":"Fengmei Yang , Yutong Chen , Yujiao Yan , Ruixin Zhao , Liran Deng , Die Tian , Meng Xie","doi":"10.1016/j.colsurfb.2026.115464","DOIUrl":"10.1016/j.colsurfb.2026.115464","url":null,"abstract":"<div><div>Pathological events in Alzheimer’s disease (AD) typically involve β-amyloid (Aβ) plaque deposition, metal ion dysregulation, oxidative stress elevation, and chronic neuroinflammation, making single-target therapies unsatisfactory. Here, we first report a biomimetic nanoplatform based on red blood cell membrane–coated cerium metal–organic frameworks (Ce-MOF-RBC) that enables multi-target synergistic intervention against AD. The Ce-MOF core exhibits potent antioxidant activity, efficiently scavenging reactive oxygen species (ROS) and restoring mitochondrial membrane potential, while its carboxylate ligands chelate Cu<sup>2 +</sup> with high efficiency (49.26 %) to inhibit Cu<sup>2+</sup>-induced Aβ fibrillation and disassemble preformed fibrils. Ce-MOF-RBC further modulates microglial phenotype, enhancing Aβ phagocytosis and reducing neuroinflammation. Importantly, RBC membrane functionalization markedly improves biological performance by prolonging systemic circulation, enhancing blood–brain barrier (BBB) penetration, and leveraging its intrinsic affinity for Aβ peptides to enrich Aβ. In vivo fluorescence imaging and brain cryosections showed that Ce-MOF-RBC achieved robust accumulation in the cortex and hippocampus, with brain fluorescence intensities 27.33-fold higher than free DiD. In the <em>C. elegans</em> AD model, Ce-MOF-RBC reduced Aβ plaque fluorescence by 32.54 %, lowered ROS levels by 45.72 %, improved chemotaxis performance (chemotaxis index increased from 34.24 % to 68.34 %), and delayed paralysis onset from 10 h to 15 h, demonstrating significant rescue of cognitive and motor deficits. In summary, these findings highlight the first demonstration of a small-sized, biomimetic Ce-MOF-RBC nanoplatform that integrates antioxidant, metal-chelating, anti-aggregation, and immunomodulatory functions, offering a promising strategy for comprehensive AD therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"262 ","pages":"Article 115464"},"PeriodicalIF":5.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-02-10DOI: 10.1016/j.colsurfb.2026.115511
R PranavKumar Shadamarshan, Harini Balaji, Harsha Shrihari Rao, K Balagangadharan, S Viji Chandran, N Selvamurugan
{"title":"Corrigendum to \"Fabrication of PCL/PVP Electrospun Fibers loaded with Trans-anethole for Bone Regeneration in vitro\" [Colloids Surf. B Biointerfaces 171 (2018) 698-706].","authors":"R PranavKumar Shadamarshan, Harini Balaji, Harsha Shrihari Rao, K Balagangadharan, S Viji Chandran, N Selvamurugan","doi":"10.1016/j.colsurfb.2026.115511","DOIUrl":"10.1016/j.colsurfb.2026.115511","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":" ","pages":"115511"},"PeriodicalIF":5.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-02-06DOI: 10.1016/j.bios.2026.118471
Aijaz Ahmad Rather , Aman Munirpasha Halikar , Ritika Sachdeva , Shivanshu Kumar Tiwari , T.R. Santhoshkumar
Precise and quantitative evaluation of CAR-T cell-induced cytotoxicity by resolving distinct cell death phenotypes in live cells remains a critical unmet need in the development of immunotherapies. Discriminating between apoptosis and necrosis induced by CAR-T therapy at single-cell resolution is essential for elucidating cytotoxic mechanisms and predicting therapeutic outcomes. Yet, most live cell assays lack the spatiotemporal sensitivity to do so. Here, we present a genetically encoded dual-biosensor system that concurrently monitors caspase-3/7 activation and mitochondrial integrity, enabling the real-time distinction between apoptosis and necrosis. The platform combines a FRET-based caspase sensor (RealCas3) and a mitochondria-targeted DsRed reporter (MitoDsRed) that exhibits compatibility with both microscopy imaging and flow cytometry. We applied this platform to functionally profile anti-EGFR CAR-T cells and uncover a biphasic cytotoxicity pattern, marked by early caspase-3/7 induced apoptosis, followed by a delayed necrosis. Notably, the system enables the time-resolved detection of secondary necrosis, a delayed immunogenic death phenotype that is often misclassified in conventional assays. The developed platform offered a robust and scalable approach for real-time functional phenotyping of immune effector responses, addressing a key gap in evaluating cell death mechanisms during immunotherapy development. By enabling the time-resolved quantification of tumor cell demise along with the mode of death, this dual-sensor system established a mechanistic framework for evaluation and rational design of safer and more effective next-generation immunotherapies.
{"title":"Genetically encoded biosensor of apoptosis and necrosis reveals the dynamics and biphasic cytotoxicity by Anti-EGFR CAR-T cells","authors":"Aijaz Ahmad Rather , Aman Munirpasha Halikar , Ritika Sachdeva , Shivanshu Kumar Tiwari , T.R. Santhoshkumar","doi":"10.1016/j.bios.2026.118471","DOIUrl":"10.1016/j.bios.2026.118471","url":null,"abstract":"<div><div>Precise and quantitative evaluation of CAR-T cell-induced cytotoxicity by resolving distinct cell death phenotypes in live cells remains a critical unmet need in the development of immunotherapies. Discriminating between apoptosis and necrosis induced by CAR-T therapy at single-cell resolution is essential for elucidating cytotoxic mechanisms and predicting therapeutic outcomes. Yet, most live cell assays lack the spatiotemporal sensitivity to do so. Here, we present a genetically encoded dual-biosensor system that concurrently monitors caspase-3/7 activation and mitochondrial integrity, enabling the real-time distinction between apoptosis and necrosis. The platform combines a FRET-based caspase sensor (RealCas3) and a mitochondria-targeted DsRed reporter (MitoDsRed) that exhibits compatibility with both microscopy imaging and flow cytometry. We applied this platform to functionally profile anti-EGFR CAR-T cells and uncover a biphasic cytotoxicity pattern, marked by early caspase-3/7 induced apoptosis, followed by a delayed necrosis. Notably, the system enables the time-resolved detection of secondary necrosis, a delayed immunogenic death phenotype that is often misclassified in conventional assays. The developed platform offered a robust and scalable approach for real-time functional phenotyping of immune effector responses, addressing a key gap in evaluating cell death mechanisms during immunotherapy development. By enabling the time-resolved quantification of tumor cell demise along with the mode of death, this dual-sensor system established a mechanistic framework for evaluation and rational design of safer and more effective next-generation immunotherapies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118471"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-06-01Epub Date: 2026-02-08DOI: 10.1016/j.bios.2026.118506
Wen-Shuo Kuo , Yen-Sung Lin , Chia-Yuan Chang , Jiu-Yao Wang , Pei-Chi Chen , Shih-Wen Tseng , Chiao-Yun Lin , Chan-Chi Chang , Shang-Rung Wu
Nitrogen (N) doping and amino functionalization markedly enhance the electron-donating capacity of graphene quantum dots (GQDs), thereby improving charge-transfer efficiency in amino-N-GQDs and yielding substantially superior photophysical performance compared with amino-free N-GQDs and N-free amino-GQDs. Further optimization was achieved through conjugation of amino-N-GQDs with sulfur- and nitrogen-rich polymers, polystyrene sulfonate and polyethylenimine, resulting in amino-N-GQD-polymer nanohybrids with significantly improved optical behavior. These hybrid nanostructures exhibited high quantum yields, excellent photostability, negligible reactive oxygen species generation, and strong two-photon luminescence, positioning them as promising contrast agents for nonlinear bioimaging. To enable molecular specificity, antibody functionalization was incorporated. When conjugated with anti-lipopolysaccharide or anti-TasA antibodies, the nanohybrids selectively targeted Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis), generating bright fluorescence, strong signal intensity, and high signal-to-noise ratios under two-photon excitation. Using a custom-built Ti:sapphire laser system operating at 970 nm (near-infrared-II region), imaging depths of up to 270 μm were achieved with ultralow excitation energies, 42.96 nJ pixel−1 for E. coli and 35.14 nJ pixel−1 for B. subtilis, acquired over 100 scans (total exposure = 0.666 s). The nanohybrids produced two-photon luminescence using only 1/49 and 1/36 of the energy required for cellular autofluorescence, corresponding to ∼2401- and ∼1296-fold signal enhancements, respectively. This remarkable efficiency supports deep, noninvasive imaging and underscores the potential of amino-N-GQD-polymer nanohybrids as versatile near-infrared-I/II-responsive probes for next-generation biomedical imaging applications.
{"title":"Graphene hybrid nanoprobes for targeted microbial sensing and ultralow-energy, deep-tissue, noninvasive multiphoton imaging in the NIR-I/II region","authors":"Wen-Shuo Kuo , Yen-Sung Lin , Chia-Yuan Chang , Jiu-Yao Wang , Pei-Chi Chen , Shih-Wen Tseng , Chiao-Yun Lin , Chan-Chi Chang , Shang-Rung Wu","doi":"10.1016/j.bios.2026.118506","DOIUrl":"10.1016/j.bios.2026.118506","url":null,"abstract":"<div><div>Nitrogen (N) doping and amino functionalization markedly enhance the electron-donating capacity of graphene quantum dots (GQDs), thereby improving charge-transfer efficiency in amino-N-GQDs and yielding substantially superior photophysical performance compared with amino-free N-GQDs and N-free amino-GQDs. Further optimization was achieved through conjugation of amino-N-GQDs with sulfur- and nitrogen-rich polymers, polystyrene sulfonate and polyethylenimine, resulting in amino-N-GQD-polymer nanohybrids with significantly improved optical behavior. These hybrid nanostructures exhibited high quantum yields, excellent photostability, negligible reactive oxygen species generation, and strong two-photon luminescence, positioning them as promising contrast agents for nonlinear bioimaging. To enable molecular specificity, antibody functionalization was incorporated. When conjugated with anti-lipopolysaccharide or anti-TasA antibodies, the nanohybrids selectively targeted <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Bacillus subtilis</em> (<em>B. subtilis</em>), generating bright fluorescence, strong signal intensity, and high signal-to-noise ratios under two-photon excitation. Using a custom-built Ti:sapphire laser system operating at 970 nm (near-infrared-II region), imaging depths of up to 270 μm were achieved with ultralow excitation energies, 42.96 nJ pixel<sup>−1</sup> for <em>E. coli</em> and 35.14 nJ pixel<sup>−1</sup> for <em>B. subtilis</em>, acquired over 100 scans (total exposure = 0.666 s). The nanohybrids produced two-photon luminescence using only 1/49 and 1/36 of the energy required for cellular autofluorescence, corresponding to ∼2401- and ∼1296-fold signal enhancements, respectively. This remarkable efficiency supports deep, noninvasive imaging and underscores the potential of amino-N-GQD-polymer nanohybrids as versatile near-infrared-I/II-responsive probes for next-generation biomedical imaging applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"301 ","pages":"Article 118506"},"PeriodicalIF":10.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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