Pub Date : 2024-12-31Epub Date: 2024-01-05DOI: 10.1080/15592324.2023.2298054
Ixchel Campos-Avelar, Amelia C Montoya-Martínez, Fannie I Parra-Cota, Sergio de Los Santos-Villalobos
The use of plant-associated microorganisms is increasingly being investigated as a key tool for mitigating the impact of biotic and abiotic threats to crops and facilitating migration to sustainable agricultural practices. The microbiome is responsible for several functions in agroecosystems, such as the transformation of organic matter, nutrient cycling, and plant/pathogen growth regulation. As climate change and global warming are altering the dynamics of plant-microbial interactions in the ecosystem, it has become essential to perform comprehensive studies to decipher current and future microbial interactions, as their useful symbiotic mechanisms could be better exploited to achieve sustainable agriculture. This will allow for the development of effective microbial inoculants that facilitate nutrient supply for the plant at its minimal energy expense, thus increasing its resilience to biotic and abiotic stresses. This article collection aims to compile state-of-the-art research focused on the elucidation and optimization of symbiotic relationships between crops and their associated microbes. The information presented here will contribute to the development of next-generation microbial inoculants for achieving a more sustainable agriculture.
{"title":"Editorial: plant-microbial symbiosis toward sustainable food security.","authors":"Ixchel Campos-Avelar, Amelia C Montoya-Martínez, Fannie I Parra-Cota, Sergio de Los Santos-Villalobos","doi":"10.1080/15592324.2023.2298054","DOIUrl":"10.1080/15592324.2023.2298054","url":null,"abstract":"<p><p>The use of plant-associated microorganisms is increasingly being investigated as a key tool for mitigating the impact of biotic and abiotic threats to crops and facilitating migration to sustainable agricultural practices. The microbiome is responsible for several functions in agroecosystems, such as the transformation of organic matter, nutrient cycling, and plant/pathogen growth regulation. As climate change and global warming are altering the dynamics of plant-microbial interactions in the ecosystem, it has become essential to perform comprehensive studies to decipher current and future microbial interactions, as their useful symbiotic mechanisms could be better exploited to achieve sustainable agriculture. This will allow for the development of effective microbial inoculants that facilitate nutrient supply for the plant at its minimal energy expense, thus increasing its resilience to biotic and abiotic stresses. This article collection aims to compile state-of-the-art research focused on the elucidation and optimization of symbiotic relationships between crops and their associated microbes. The information presented here will contribute to the development of next-generation microbial inoculants for achieving a more sustainable agriculture.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139107022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-06-14DOI: 10.1080/23288604.2024.2352885
Elina Dale, Julia Novak, Denys Dmytriiev, Olga Demeshko, Jarno Habicht
This commentary examines the resilience of primary health care in Ukraine amidst the ongoing war, drawing a few reflections relevant for other fragile and conflict-affected situations. Using personal observations and various published and unpublished reports, this article outlines five reflections on the strengths, challenges, and necessary adaptations of Primary Health Care (PHC) in Ukraine. It underscores the concerted efforts of the government to maintain public financing of PHC, thereby averting system collapse. The research also highlights the role of strategic adaptations during the COVID-19 pandemic in fostering resilience during the war, including the widespread use of digital communication and skills training. The commentary emphasizes the role of managerial and financial autonomy in facilitating quick and efficient organizational response to crisis. It also recognizes emerging challenges, including better access to PHC services among the internally displaced persons, shifting patient profiles and service needs, and challenges related to reliance on local government financing. Finally, the authors advocate for a coordinated approach in humanitarian response, recovery efforts, and development programs to ensure the sustainability and effectiveness of PHC in Ukraine.
{"title":"Resilience of Primary Health Care in Ukraine: Challenges of the Pandemic and War.","authors":"Elina Dale, Julia Novak, Denys Dmytriiev, Olga Demeshko, Jarno Habicht","doi":"10.1080/23288604.2024.2352885","DOIUrl":"https://doi.org/10.1080/23288604.2024.2352885","url":null,"abstract":"<p><p>This commentary examines the resilience of primary health care in Ukraine amidst the ongoing war, drawing a few reflections relevant for other fragile and conflict-affected situations. Using personal observations and various published and unpublished reports, this article outlines five reflections on the strengths, challenges, and necessary adaptations of Primary Health Care (PHC) in Ukraine. It underscores the concerted efforts of the government to maintain public financing of PHC, thereby averting system collapse. The research also highlights the role of strategic adaptations during the COVID-19 pandemic in fostering resilience during the war, including the widespread use of digital communication and skills training. The commentary emphasizes the role of managerial and financial autonomy in facilitating quick and efficient organizational response to crisis. It also recognizes emerging challenges, including better access to PHC services among the internally displaced persons, shifting patient profiles and service needs, and challenges related to reliance on local government financing. Finally, the authors advocate for a coordinated approach in humanitarian response, recovery efforts, and development programs to ensure the sustainability and effectiveness of PHC in Ukraine.</p>","PeriodicalId":73218,"journal":{"name":"Health systems and reform","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-03-24DOI: 10.1080/15592324.2024.2329841
Felix Lutter, Wolfram Brenner, Franziska Krajinski-Barth, Vajiheh Safavi-Rizi
Nitric oxide (NO) and cytokinins (CKs) are known for their crucial contributions to plant development, growth, senescence, and stress response. Despite the importance of both signals in stress responses, their interaction remains largely unexplored. The interplay between NO and CKs emerges as particularly significant not only regarding plant growth and development but also in addressing plant stress response, particularly in the context of extreme weather events leading to yield loss. In this review, we summarize NO and CKs metabolism and signaling. Additionally, we emphasize the crosstalk between NO and CKs, underscoring its potential impact on stress response, with a focus on hypoxia tolerance. Finally, we address the most urgent questions that demand answers and offer recommendations for future research endeavors.
众所周知,一氧化氮(NO)和细胞分裂素(CKs)对植物的发育、生长、衰老和胁迫响应有着至关重要的贡献。尽管这两种信号在胁迫响应中都很重要,但它们之间的相互作用在很大程度上仍未得到探索。NO 和 CKs 之间的相互作用不仅对植物的生长和发育具有重要意义,而且对植物的胁迫响应也具有重要意义,尤其是在极端天气事件导致减产的情况下。在这篇综述中,我们总结了 NO 和 CKs 的新陈代谢和信号转导。此外,我们还强调了 NO 和 CKs 之间的相互影响,强调了其对胁迫响应的潜在影响,重点是缺氧耐受性。最后,我们探讨了亟待解决的问题,并对未来的研究工作提出了建议。
{"title":"Nitric oxide and cytokinin cross-talk and their role in plant hypoxia response.","authors":"Felix Lutter, Wolfram Brenner, Franziska Krajinski-Barth, Vajiheh Safavi-Rizi","doi":"10.1080/15592324.2024.2329841","DOIUrl":"10.1080/15592324.2024.2329841","url":null,"abstract":"<p><p>Nitric oxide (NO) and cytokinins (CKs) are known for their crucial contributions to plant development, growth, senescence, and stress response. Despite the importance of both signals in stress responses, their interaction remains largely unexplored. The interplay between NO and CKs emerges as particularly significant not only regarding plant growth and development but also in addressing plant stress response, particularly in the context of extreme weather events leading to yield loss. In this review, we summarize NO and CKs metabolism and signaling. Additionally, we emphasize the crosstalk between NO and CKs, underscoring its potential impact on stress response, with a focus on hypoxia tolerance. Finally, we address the most urgent questions that demand answers and offer recommendations for future research endeavors.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140195413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-06-03DOI: 10.1080/15592324.2024.2359258
Peng Wei, Haitao Li, Yanyou Wu, Cheng Zhang
Tea plantations in Karst regions suffer from the serious effects of frequent temporary karst droughts, leading to a decline in tea production and quality in the region. The close relationship between growth and electrical parameters of plants, including physiological capacitance, resistance and impedance, can be used to accurately monitor their plant water status online, quickly, accurately, timely and nondestructively. In this study, three tea tree cultivars of Zhonghuang No.2 (ZH), Wuniuzao (WNZ), and Longjing 43 (LJ) with different levels of drought resistance were selected as experimental materials, and experiments were carried out under controlled conditions according to control (soil water content of 40-45%, D0), (keeping D0 no watering to 5 days, D5), (keeping D0 no watering to 10 days, D10), (the first day after D10 is rehydrated to D0 is regarded as R1) and (the fifth day after D10 rehydration to D0 is regarded as R5), to determine intracellular water metabolism and nutrient translocation characteristics based on intrinsic electrical parameters. The photosynthetic characteristics and chlorophyll fluorescence parameters were also determined to investigate the response of water metabolism to simulated karst drought in the three tea tree cultivars. The results indicated that the water metabolism patterns responded to environmental water changes with a medium water-holding capacity, medium water transport rate, and low water-use efficiency, and the nutrient patterns in those tea tree varieties demonstrated with a high nutrient flux per unit area, low nutrient transfer rate, and high nutrient transport capacity. After rehydration, only the electrical characteristics of WNZ returned to the D0 levels, but the net photosynthetic rate of all varieties returned to or even exceeded the D0 levels. The chlorophyll fluorescence parameters could not be used to characterize the recoverability of metabolism in tea trees. The electrical characteristics quickly reflected the response of the water metabolism in plants to environmental changes, and the fusion of electrical characteristics and photosynthetic characteristics was able to more quickly, accurately, and comprehensively reflect the response of water metabolism to temporary karst drought.
{"title":"Association of the electrical parameters and photosynthetic characteristics of the tea tree manifests its response to simulated karst drought.","authors":"Peng Wei, Haitao Li, Yanyou Wu, Cheng Zhang","doi":"10.1080/15592324.2024.2359258","DOIUrl":"10.1080/15592324.2024.2359258","url":null,"abstract":"<p><p>Tea plantations in Karst regions suffer from the serious effects of frequent temporary karst droughts, leading to a decline in tea production and quality in the region. The close relationship between growth and electrical parameters of plants, including physiological capacitance, resistance and impedance, can be used to accurately monitor their plant water status online, quickly, accurately, timely and nondestructively. In this study, three tea tree cultivars of Zhonghuang No.2 (ZH), Wuniuzao (WNZ), and Longjing 43 (LJ) with different levels of drought resistance were selected as experimental materials, and experiments were carried out under controlled conditions according to control (soil water content of 40-45%, D0), (keeping D0 no watering to 5 days, D5), (keeping D0 no watering to 10 days, D10), (the first day after D10 is rehydrated to D0 is regarded as R1) and (the fifth day after D10 rehydration to D0 is regarded as R5), to determine intracellular water metabolism and nutrient translocation characteristics based on intrinsic electrical parameters. The photosynthetic characteristics and chlorophyll fluorescence parameters were also determined to investigate the response of water metabolism to simulated karst drought in the three tea tree cultivars. The results indicated that the water metabolism patterns responded to environmental water changes with a medium water-holding capacity, medium water transport rate, and low water-use efficiency, and the nutrient patterns in those tea tree varieties demonstrated with a high nutrient flux per unit area, low nutrient transfer rate, and high nutrient transport capacity. After rehydration, only the electrical characteristics of WNZ returned to the D0 levels, but the net photosynthetic rate of all varieties returned to or even exceeded the D0 levels. The chlorophyll fluorescence parameters could not be used to characterize the recoverability of metabolism in tea trees. The electrical characteristics quickly reflected the response of the water metabolism in plants to environmental changes, and the fusion of electrical characteristics and photosynthetic characteristics was able to more quickly, accurately, and comprehensively reflect the response of water metabolism to temporary karst drought.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During plant growth and development, the YABBY gene plays a crucial role in the morphological structure, hormone signaling, stress resistance, crop breeding, and agricultural production of plant lateral organs, leaves, flowers, and fruits. Astragalus mongholicus is a perennial herbaceous plant in the legume family, widely used worldwide due to its high medicinal and edible value. However, there have been no reports of the YABBY gene family in A. mongholicus. This study used bioinformatics methods, combined with databases and analysis websites, to systematically analyze the AmYABBY gene family in the entire genome of A. mongholicus and verified its expression patterns in different tissues of A. mongholicus through transcriptome data and qRT-PCR experiments. A total of seven AmYABBY genes were identified, which can be divided into five subfamilies and distributed on three chromosomes. Two pairs of AmYABBY genes may be involved in fragment duplication on three chromosomes. All AmYABBY proteins have a zinc finger YABBY domain, and members of the same group have similar motif composition and intron - exon structure. In the promoter region of the genes, light-responsive and MeJa-response cis-elements are dominant. AmYABBY is highly expressed in stems and leaves, especially AmYABBY1, AmYABBY2, and AmYABBY3, which play important roles in the growth and development of stems and leaves. The AmYABBY gene family regulates the growth and development of A. mongholicus. In summary, this study provides a theoretical basis for in-depth research on the function of the AmYABBY gene and new insights into the molecular response mechanism of the growth and development of the traditional Chinese medicine A. mongholicus.
{"title":"Genome-wide identification of <i>YABBY</i> gene family and its expression pattern analysis in <i>Astragalus mongholicus</i>.","authors":"Jiamei Wang, Zhen Wang, Panpan Wang, Jianhao Wu, Lingyang Kong, Lengleng Ma, Shan Jiang, Weichao Ren, Weili Liu, Yanli Guo, Wei Ma, Xiubo Liu","doi":"10.1080/15592324.2024.2355740","DOIUrl":"10.1080/15592324.2024.2355740","url":null,"abstract":"<p><p>During plant growth and development, the <i>YABBY</i> gene plays a crucial role in the morphological structure, hormone signaling, stress resistance, crop breeding, and agricultural production of plant lateral organs, leaves, flowers, and fruits. <i>Astragalus mongholicus</i> is a perennial herbaceous plant in the legume family, widely used worldwide due to its high medicinal and edible value. However, there have been no reports of the <i>YABBY</i> gene family in <i>A. mongholicus</i>. This study used bioinformatics methods, combined with databases and analysis websites, to systematically analyze the <i>AmYABBY</i> gene family in the entire genome of <i>A. mongholicus</i> and verified its expression patterns in different tissues of <i>A. mongholicus</i> through transcriptome data and qRT-PCR experiments. A total of seven <i>AmYABBY</i> genes were identified, which can be divided into five subfamilies and distributed on three chromosomes. Two pairs of <i>AmYABBY</i> genes may be involved in fragment duplication on three chromosomes. All AmYABBY proteins have a zinc finger YABBY domain, and members of the same group have similar motif composition and intron - exon structure. In the promoter region of the genes, light-responsive and MeJa-response <i>cis</i>-elements are dominant. <i>AmYABBY</i> is highly expressed in stems and leaves, especially <i>AmYABBY1</i>, <i>AmYABBY2</i>, and <i>AmYABBY3</i>, which play important roles in the growth and development of stems and leaves. The <i>AmYABBY</i> gene family regulates the growth and development of <i>A. mongholicus</i>. In summary, this study provides a theoretical basis for in-depth research on the function of the <i>AmYABBY</i> gene and new insights into the molecular response mechanism of the growth and development of the traditional Chinese medicine <i>A. mongholicus</i>.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11123558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Colonic mucosal pseudolipomatosis is a rare and benign endoscopic finding with distinct macroscopic and histological characteristics. Case series: We observed a form of unprecedented colitis in eight patients in a 3-month period. Operators have found, during colonoscopy, flat or slightly raised whitish-yellow plaques, in the colonic mucosa of all patients. Histological examination concluded to pseudolipomatosis. After investigation, the disinfectant machine was found to have technical malfunctioning of the rinse cycle of the endoscope during this period. No other cases were observed after the machine was fixed. Conclusion: Pseudolipomatosis is more an endoscopically induced lesion than a true pathological condition. A careful check of the disinfection process should be carried out when such lesions are detected.
{"title":"Endoscope disinfectant-induced colonic pseudolipomatosis: case series of a rare condition.","authors":"Charfeddine Baccouche, Myriam Ayari, Imen Abdelaali, Amen Dhaoui, Taieb Jomni, Mohamed Hedi Douggui","doi":"10.2144/fsoa-2023-0210","DOIUrl":"10.2144/fsoa-2023-0210","url":null,"abstract":"<p><p><b>Aim:</b> Colonic mucosal pseudolipomatosis is a rare and benign endoscopic finding with distinct macroscopic and histological characteristics. <b>Case series:</b> We observed a form of unprecedented colitis in eight patients in a 3-month period. Operators have found, during colonoscopy, flat or slightly raised whitish-yellow plaques, in the colonic mucosa of all patients. Histological examination concluded to pseudolipomatosis. After investigation, the disinfectant machine was found to have technical malfunctioning of the rinse cycle of the endoscope during this period. No other cases were observed after the machine was fixed. <b>Conclusion:</b> Pseudolipomatosis is more an endoscopically induced lesion than a true pathological condition. A careful check of the disinfection process should be carried out when such lesions are detected.</p>","PeriodicalId":12568,"journal":{"name":"Future Science OA","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11172253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-06-26DOI: 10.1080/15592324.2024.2371693
Anetta Kuczyńska, Martyna Michałek, Piotr Ogrodowicz, Michał Kempa, Natalia Witaszak, Michał Dziurka, Damian Gruszka, Agata Daszkowska-Golec, Iwona Szarejko, Paweł Krajewski, Krzysztof Mikołajczak
One of the main signal transduction pathways that modulate plant growth and stress responses, including drought, is the action of phytohormones. Recent advances in omics approaches have facilitated the exploration of plant genomes. However, the molecular mechanisms underlying the response in the crown of barley, which plays an essential role in plant performance under stress conditions and regeneration after stress treatment, remain largely unclear. The objective of the present study was the elucidation of drought-induced molecular reactions in the crowns of different barley phytohormone mutants. We verified the hypothesis that defects of gibberellins, brassinosteroids, and strigolactones action affect the transcriptomic, proteomic, and hormonal response of barley crown to the transitory drought influencing plant development under stress. Moreover, we assumed that due to the strong connection between strigolactones and branching the hvdwarf14.d mutant, with dysfunctional receptor of strigolactones, manifests the most abundant alternations in crowns and phenotype under drought. Finally, we expected to identify components underlying the core response to drought which are independent of the genetic background. Large-scale analyses were conducted using gibberellins-biosynthesis, brassinosteroids-signaling, and strigolactones-signaling mutants, as well as reference genotypes. Detailed phenotypic evaluation was also conducted. The obtained results clearly demonstrated that hormonal disorders caused by mutations in the HvGA20ox2, HvBRI1, and HvD14 genes affected the multifaceted reaction of crowns to drought, although the expression of these genes was not induced by stress. The study further detected not only genes and proteins that were involved in the drought response and reacted specifically in mutants compared to the reaction of reference genotypes and vice versa, but also the candidates that may underlie the genotype-universal stress response. Furthermore, candidate genes involved in phytohormonal interactions during the drought response were identified. We also found that the interplay between hormones, especially gibberellins and auxins, as well as strigolactones and cytokinins may be associated with the regulation of branching in crowns exposed to drought. Overall, the present study provides novel insights into the molecular drought-induced responses that occur in barley crowns.
{"title":"Drought-induced molecular changes in crown of various barley phytohormone mutants.","authors":"Anetta Kuczyńska, Martyna Michałek, Piotr Ogrodowicz, Michał Kempa, Natalia Witaszak, Michał Dziurka, Damian Gruszka, Agata Daszkowska-Golec, Iwona Szarejko, Paweł Krajewski, Krzysztof Mikołajczak","doi":"10.1080/15592324.2024.2371693","DOIUrl":"10.1080/15592324.2024.2371693","url":null,"abstract":"<p><p>One of the main signal transduction pathways that modulate plant growth and stress responses, including drought, is the action of phytohormones. Recent advances in omics approaches have facilitated the exploration of plant genomes. However, the molecular mechanisms underlying the response in the crown of barley, which plays an essential role in plant performance under stress conditions and regeneration after stress treatment, remain largely unclear. The objective of the present study was the elucidation of drought-induced molecular reactions in the crowns of different barley phytohormone mutants. We verified the hypothesis that defects of gibberellins, brassinosteroids, and strigolactones action affect the transcriptomic, proteomic, and hormonal response of barley crown to the transitory drought influencing plant development under stress. Moreover, we assumed that due to the strong connection between strigolactones and branching the <i>hvdwarf14.d</i> mutant, with dysfunctional receptor of strigolactones, manifests the most abundant alternations in crowns and phenotype under drought. Finally, we expected to identify components underlying the core response to drought which are independent of the genetic background. Large-scale analyses were conducted using gibberellins-biosynthesis, brassinosteroids-signaling, and strigolactones-signaling mutants, as well as reference genotypes. Detailed phenotypic evaluation was also conducted. The obtained results clearly demonstrated that hormonal disorders caused by mutations in the <i>HvGA20ox2</i>, <i>HvBRI1</i>, and <i>HvD14</i> genes affected the multifaceted reaction of crowns to drought, although the expression of these genes was not induced by stress. The study further detected not only genes and proteins that were involved in the drought response and reacted specifically in mutants compared to the reaction of reference genotypes and <i>vice versa</i>, but also the candidates that may underlie the genotype-universal stress response. Furthermore, candidate genes involved in phytohormonal interactions during the drought response were identified. We also found that the interplay between hormones, especially gibberellins and auxins, as well as strigolactones and cytokinins may be associated with the regulation of branching in crowns exposed to drought. Overall, the present study provides novel insights into the molecular drought-induced responses that occur in barley crowns.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-05-14DOI: 10.1080/15592324.2024.2349868
Qilin Yang, Jinheng Huang, Xiaofeng Nie, XiaoMin Tang, Peiran Liao, Quan Yang
The purpose of this study was to analyze the role of transcription factor in Desmodium styracifolium, proving that the DsWRKY6 transcription factor was related to the plant phenotypes of Desmodium styracifolium - cv. 'GuangYaoDa1' and it could be used in molecular-assisted breeding. 'GuangYaoDa1' was used as the material and its DNA was the template to clone DsWRKY6, the transgenic Arabidopsis thaliana line was constructed by agrobacterium tumefaciens‑mediated transformation. Transgenic Arabidopsis thaliana was cultivated to study phenotype and physiological and biochemical indexes. Phenotypic observation showed that DsWRKY6 transgenic Arabidopsis thaliana had a faster growth rate while compared with the control group, they had longer lengths of main stem, lateral branches of cauline leaves, and root, but a lower number of cauline leaves and lateral branches of cauline leaves. And it also showed that their flowering and fruiting periods were advanced. The results of physiological and biochemical indexes showed that the relative expressions of DsWRKY6 increased and the abscisic acid content significantly increased in DsWRKY6 transgenic Arabidopsis thaliana compared with the control group. According to the above results, DsWRKY6 could regulate the advancing of flowering and fruiting periods caused by the improvement of abscisic acid content, and expression of the DsWRKY6 transcription factor might be the cause of the upright growth of 'GuangYaoDa1'.
{"title":"Cloning and functional validation of <i>DsWRKY6</i> gene from <i>Desmodium styracifolium</i>.","authors":"Qilin Yang, Jinheng Huang, Xiaofeng Nie, XiaoMin Tang, Peiran Liao, Quan Yang","doi":"10.1080/15592324.2024.2349868","DOIUrl":"10.1080/15592324.2024.2349868","url":null,"abstract":"<p><p>The purpose of this study was to analyze the role of transcription factor in <i>Desmodium styracifolium</i>, proving that the <i>DsWRKY6</i> transcription factor was related to the plant phenotypes of <i>Desmodium styracifolium</i> - cv. 'GuangYaoDa1' and it could be used in molecular-assisted breeding. 'GuangYaoDa1' was used as the material and its DNA was the template to clone DsWRKY6, the transgenic <i>Arabidopsis thaliana</i> line was constructed by agrobacterium tumefaciens‑mediated transformation. Transgenic <i>Arabidopsis thaliana</i> was cultivated to study phenotype and physiological and biochemical indexes. Phenotypic observation showed that <i>DsWRKY6</i> transgenic <i>Arabidopsis thaliana</i> had a faster growth rate while compared with the control group, they had longer lengths of main stem, lateral branches of cauline leaves, and root, but a lower number of cauline leaves and lateral branches of cauline leaves. And it also showed that their flowering and fruiting periods were advanced. The results of physiological and biochemical indexes showed that the relative expressions of <i>DsWRKY6</i> increased and the abscisic acid content significantly increased in <i>DsWRKY6</i> transgenic <i>Arabidopsis thaliana</i> compared with the control group. According to the above results, <i>DsWRKY6</i> could regulate the advancing of flowering and fruiting periods caused by the improvement of abscisic acid content, and expression of the <i>DsWRKY6</i> transcription factor might be the cause of the upright growth of 'GuangYaoDa1'.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reducing the price of expensive medical products through centralized procurement is generally considered an effective way to save public medical resources. Against this background, this paper presents an analysis of the impact of centralized procurement in China by comparing the treatment costs and patterns for acute myocardial infarction (AMI) patients before and after the introduction of this method of purchasing, with specific reference to the use of coronary stents. We found that, after the implementation of centralized procurement for coronary stents, the total expenditure of AMI cases receiving percutaneous coronary interventions with stent implantation (PCI with stents) dropped by 23.4%. The use rate of PCI with stents decreased by 32.5%, with the most significant decrease being evident in cases in which two stents were used simultaneously (32.9%). Meanwhile, percutaneous coronary interventions with balloon implantation (PCI with balloons) increased by 31.5% and coronary artery bypass grafting (CABG) increased by 80.3%. Based on these patterns, it can be observed that the use of centralized procurement significantly reduced the profits of the relevant medical manufacturers, forcing them to decrease their marketing investments, weakening their influence on providers, and ultimately resulting in a more principled use of coronary stents. We therefore conclude that, with reference to the data cited, the centralized procurement program led not only to a reduction in procurement prices but also to decreased overuse of these expensive medical products.
通过集中采购降低昂贵医疗产品的价格被普遍认为是节约公共医疗资源的有效途径。在此背景下,本文通过比较集中采购前后急性心肌梗死(AMI)患者的治疗费用和模式,特别是冠状动脉支架的使用情况,分析了集中采购在中国的影响。我们发现,在实施冠状动脉支架集中采购后,接受经皮冠状动脉介入治疗并植入支架(PCI with stents)的急性心肌梗死病例的总支出下降了 23.4%。植入支架的经皮冠状动脉介入治疗的使用率下降了 32.5%,其中同时使用两个支架的病例下降最为明显(32.9%)。同时,球囊植入经皮冠状动脉介入治疗(PCI with balloons)增加了 31.5%,冠状动脉旁路移植术(CABG)增加了 80.3%。根据这些模式可以看出,集中采购的使用大大降低了相关医疗制造商的利润,迫使他们减少营销投入,削弱了他们对医疗服务提供者的影响力,最终导致冠状动脉支架的使用更加原则化。因此,我们得出结论,根据所引用的数据,集中采购计划不仅降低了采购价格,还减少了这些昂贵医疗产品的过度使用。
{"title":"The Impact of Centralized Procurement on Treatment Patterns for Myocardial Infarction and More Principled Utilization of Coronary Stents.","authors":"Weiyan Jian, Shanshan Huo, Lanyue Zhang, Wuping Zhou","doi":"10.1080/23288604.2024.2366167","DOIUrl":"https://doi.org/10.1080/23288604.2024.2366167","url":null,"abstract":"<p><p>Reducing the price of expensive medical products through centralized procurement is generally considered an effective way to save public medical resources. Against this background, this paper presents an analysis of the impact of centralized procurement in China by comparing the treatment costs and patterns for acute myocardial infarction (AMI) patients before and after the introduction of this method of purchasing, with specific reference to the use of coronary stents. We found that, after the implementation of centralized procurement for coronary stents, the total expenditure of AMI cases receiving percutaneous coronary interventions with stent implantation (PCI with stents) dropped by 23.4%. The use rate of PCI with stents decreased by 32.5%, with the most significant decrease being evident in cases in which two stents were used simultaneously (32.9%). Meanwhile, percutaneous coronary interventions with balloon implantation (PCI with balloons) increased by 31.5% and coronary artery bypass grafting (CABG) increased by 80.3%. Based on these patterns, it can be observed that the use of centralized procurement significantly reduced the profits of the relevant medical manufacturers, forcing them to decrease their marketing investments, weakening their influence on providers, and ultimately resulting in a more principled use of coronary stents. We therefore conclude that, with reference to the data cited, the centralized procurement program led not only to a reduction in procurement prices but also to decreased overuse of these expensive medical products.</p>","PeriodicalId":73218,"journal":{"name":"Health systems and reform","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-31Epub Date: 2024-04-28DOI: 10.1080/15592324.2024.2335025
Shengxiao Wang, Chenyue Wang, Jun Zhang, Kan Jiang, Fang Nian
Allelopathy is the main chemical means in the invasion process of exotic plants and one of the key factors in grassland degradation. In this experiment, we investigated the effects of ethyl acetate phase extract (EAE), n-butanol phase extract (BE) and aqueous phase extract (AE) from the aboveground (stems and leaves) and roots of Ligularia sagitta on seed germination and seedling growth of four Gramineae forages (Poa pratensis L. Festuca ovina L. Elymus nutans Griseb. Agropyron cristatum (L.) Gaertn.) in their sympatric domains and one Legosuminae forage (Medicago sativa L.). The chemical components in each phase extract of L. sagitta were determined with UHPLC-MS/MS non-targeted metabolomics, and the differential compounds were screened using Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA). Within a set concentration range, EAE significantly inhibited seed germination and seedling growth of four Gramineae forages. BE and AE acted mainly in the seedling growth stage and did not significantly inhibit forage seed germination. P. pratensis was most sensitive to L. sagitta extracts; at 2.0 mg/mL of EAE from roots, germination energy and germination rate of P. pratensis seeds were 0. L. sagitta extracts inhibited the growth of M. sativa seedlings and did not inhibit its seed germination. A total of 904 compounds were identified with UHPLC-MS/MS, among which 31, 64, 81 and 66 metabolites displayed different accumulation patterns in the four comparison groups (R.EAE vs. R.BE, R.EAE vs. R.AE, SL.EAE vs. SL.BE, SL.EAE vs. SL.AE), respectively. In particular, 9 compounds were found to be common up-regulated differential metabolites in the four comparison groups and were enriched in EAE. Additionally, N,N-dimethylaniline, Caffeic acid, 4-Hydroxybenzoic acid, 4-Hydroxybenzaldehyde and cis-9-Octadecenoic acid as potential allelochemicals in L. sagitta. The results of this study support efforts at finding alternative control plants for the restoration of poisonous grass-type degraded grasslands.
{"title":"Allelopathy and potential allelochemicals of <i>Ligularia sagitta</i> as an invasive plant.","authors":"Shengxiao Wang, Chenyue Wang, Jun Zhang, Kan Jiang, Fang Nian","doi":"10.1080/15592324.2024.2335025","DOIUrl":"https://doi.org/10.1080/15592324.2024.2335025","url":null,"abstract":"<p><p>Allelopathy is the main chemical means in the invasion process of exotic plants and one of the key factors in grassland degradation. In this experiment, we investigated the effects of ethyl acetate phase extract (EAE), n-butanol phase extract (BE) and aqueous phase extract (AE) from the aboveground (stems and leaves) and roots of <i>Ligularia sagitta</i> on seed germination and seedling growth of four <i>Gramineae</i> forages (<i>Poa pratensis</i> L. <i>Festuca ovina</i> L. <i>Elymus nutans</i> Griseb. <i>Agropyron cristatum</i> (L.) Gaertn.) in their sympatric domains and one <i>Legosuminae</i> forage (<i>Medicago sativa</i> L.). The chemical components in each phase extract of <i>L. sagitta</i> were determined with UHPLC-MS/MS non-targeted metabolomics, and the differential compounds were screened using Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA). Within a set concentration range, EAE significantly inhibited seed germination and seedling growth of four <i>Gramineae</i> forages. BE and AE acted mainly in the seedling growth stage and did not significantly inhibit forage seed germination. <i>P. pratensis</i> was most sensitive to <i>L. sagitta</i> extracts; at 2.0 mg/mL of EAE from roots, germination energy and germination rate of <i>P. pratensis</i> seeds were 0. <i>L. sagitta</i> extracts inhibited the growth of <i>M. sativa</i> seedlings and did not inhibit its seed germination. A total of 904 compounds were identified with UHPLC-MS/MS, among which 31, 64, 81 and 66 metabolites displayed different accumulation patterns in the four comparison groups (R.EAE vs. R.BE, R.EAE vs. R.AE, SL.EAE vs. SL.BE, SL.EAE vs. SL.AE), respectively. In particular, 9 compounds were found to be common up-regulated differential metabolites in the four comparison groups and were enriched in EAE. Additionally, N,N-dimethylaniline, Caffeic acid, 4-Hydroxybenzoic acid, 4-Hydroxybenzaldehyde and cis-9-Octadecenoic acid as potential allelochemicals in <i>L. sagitta</i>. The results of this study support efforts at finding alternative control plants for the restoration of poisonous grass-type degraded grasslands.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11057658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}