Pub Date : 2025-12-01DOI: 10.1016/j.nanoms.2022.10.001
Peixun Xiong , Jeiwan Tan , Hongdae Lee , Neul Ha , Sang Joon Lee , Wooseok Yang , Ho Seok Park
The continuous depletion of fossil fuels and the effects of climate change have encouraged prompt action to attain carbon neutrality. Technologies that transform and store renewable energy are crucial for creating a sustainable society, which is independent of fossil fuels. In this regard, electrochemical water splitting based on the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) is an attractive technique for producing carbon-free hydrogen fuels. Additionally, rechargeable metal–air batteries (MABs) are another intriguing way for renewable energy storage through reversible oxygen reactions (OER and the oxygen reduction reaction, ORR). Herein, we comprehensively review bifunctional electrocatalysts for water splitting (HER and OER) and MABs (OER and ORR), particularly 2D carbon material-derived heterostructures. The synthesis and properties of 2D carbon materials and their energy conversion and storage mechanisms are discussed to highlight the bifunctionality of the heterostructures. Recent studies on bifunctional electrocatalysts based on 2D carbon-derived heterostructures are also reviewed. Finally, perspectives for future studies and multifunctional catalysts are presented.
{"title":"Two-dimensional carbon-based heterostructures as bifunctional electrocatalysts for water splitting and metal–air batteries","authors":"Peixun Xiong , Jeiwan Tan , Hongdae Lee , Neul Ha , Sang Joon Lee , Wooseok Yang , Ho Seok Park","doi":"10.1016/j.nanoms.2022.10.001","DOIUrl":"10.1016/j.nanoms.2022.10.001","url":null,"abstract":"<div><div>The continuous depletion of fossil fuels and the effects of climate change have encouraged prompt action to attain carbon neutrality. Technologies that transform and store renewable energy are crucial for creating a sustainable society, which is independent of fossil fuels. In this regard, electrochemical water splitting based on the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) is an attractive technique for producing carbon-free hydrogen fuels. Additionally, rechargeable metal–air batteries (MABs) are another intriguing way for renewable energy storage through reversible oxygen reactions (OER and the oxygen reduction reaction, ORR). Herein, we comprehensively review bifunctional electrocatalysts for water splitting (HER and OER) and MABs (OER and ORR), particularly 2D carbon material-derived heterostructures. The synthesis and properties of 2D carbon materials and their energy conversion and storage mechanisms are discussed to highlight the bifunctionality of the heterostructures. Recent studies on bifunctional electrocatalysts based on 2D carbon-derived heterostructures are also reviewed. Finally, perspectives for future studies and multifunctional catalysts are presented.</div></div>","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 735-760"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45106907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01DOI: 10.1186/s40104-025-01291-w
Youyoung Choi, Mi Zhou, Atmir Romero-Pérez, Karen A Beauchemin, Stephane Duval, Maik Kindermann, Le Luo Guan
Background: The enteric methane inhibitor 3-nitrooxypropanol (3-NOP) inhibits the key enzyme in ruminal methanogenesis, but whether short-term (ST) and long-term (LT) dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied. This study investigated changes in rumen bacteria, archaea, and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.
Results: A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation. The ST study (95 samples) used eight ruminally cannulated beef cattle in a 4 × 4 Latin square design with four 28-d of 3-NOP treatments [mg/kg of dry matter (DM)]: control: 0, low: 53, med: 161, and high: 345. The LT study (48 samples) was a completely randomized design with two 3-NOP treatments [control: 0, and high: 280 mg/kg of DM) fed for 112-d followed by a 16-d withdrawal (without 3-NOP). Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed. Under ST supplementation, the relative abundances of Prevotella, Methanobrevibacter (Mbb.) ruminantium, Methanosphaera sp. ISO3-F5, and Entodinium were increased (Q < 0.05), whereas those of Mbb. gottschalkii and Epidinium were decreased (Q < 0.05) with 3-NOP supplementation. In LT study, relative abundances of Mbb. ruminantium, and Methanosphaera sp. Group5 were increased (Q < 0.05), while those of Saccharofermentans and Mbb. gottschalkii were decreased (Q < 0.05) with 3-NOP supplementation. Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp. Group5 (P < 0.05) after 3-NOP withdrawal. Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher (P = 0.029) relative abundances of Eggerthellaceae DNF00809, p-1088-a5 gut group, and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa. Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.
Conclusions: Both ST and LT supplementation affected overall rumen microbial profile, with individual microbial groups responded to 3-NOP supplementation differently. After 3-NOP withdrawal, not all microbes showed recovery, indicating that the 3-NOP driven shifts were only partially reversible. These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.
{"title":"Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community.","authors":"Youyoung Choi, Mi Zhou, Atmir Romero-Pérez, Karen A Beauchemin, Stephane Duval, Maik Kindermann, Le Luo Guan","doi":"10.1186/s40104-025-01291-w","DOIUrl":"10.1186/s40104-025-01291-w","url":null,"abstract":"<p><strong>Background: </strong>The enteric methane inhibitor 3-nitrooxypropanol (3-NOP) inhibits the key enzyme in ruminal methanogenesis, but whether short-term (ST) and long-term (LT) dietary supplementation has similar effects on rumen microbiota in beef cattle and how microbes change after 3-NOP withdrawal have not been studied. This study investigated changes in rumen bacteria, archaea, and protozoa after ST and LT dietary supplementation and removal of 3-NOP using metataxonomic analysis.</p><p><strong>Results: </strong>A total of 143 rumen samples were collected from two beef cattle studies with 3-NOP supplementation. The ST study (95 samples) used eight ruminally cannulated beef cattle in a 4 × 4 Latin square design with four 28-d of 3-NOP treatments [mg/kg of dry matter (DM)]: control: 0, low: 53, med: 161, and high: 345. The LT study (48 samples) was a completely randomized design with two 3-NOP treatments [control: 0, and high: 280 mg/kg of DM) fed for 112-d followed by a 16-d withdrawal (without 3-NOP). Bacterial and archaeal communities were significantly affected by 3-NOP supplementation but limited effects on protozoal communities were observed. Under ST supplementation, the relative abundances of Prevotella, Methanobrevibacter (Mbb.) ruminantium, Methanosphaera sp. ISO3-F5, and Entodinium were increased (Q < 0.05), whereas those of Mbb. gottschalkii and Epidinium were decreased (Q < 0.05) with 3-NOP supplementation. In LT study, relative abundances of Mbb. ruminantium, and Methanosphaera sp. Group5 were increased (Q < 0.05), while those of Saccharofermentans and Mbb. gottschalkii were decreased (Q < 0.05) with 3-NOP supplementation. Comparison between 3-NOP supplementation and the withdrawal revealed increased relative abundances of Clostridia UCG-014 and Oscillospiraceae NK4A214 group and decreased those of Eubacterium nodatum group and Methanosphaera sp. Group5 (P < 0.05) after 3-NOP withdrawal. Further comparison of rumen microbiota between control and 3-NOP withdrawal showed significantly higher (P = 0.029) relative abundances of Eggerthellaceae DNF00809, p-1088-a5 gut group, and Family XII UCG-001 in control group while no significant differences were detected for archaea and protozoa. Microbial network analysis revealed that microbial interactions differed by both 3-NOP dose and durations.</p><p><strong>Conclusions: </strong>Both ST and LT supplementation affected overall rumen microbial profile, with individual microbial groups responded to 3-NOP supplementation differently. After 3-NOP withdrawal, not all microbes showed recovery, indicating that the 3-NOP driven shifts were only partially reversible. These findings provide an understanding of the effects of 3-NOP on rumen microbial communities and their adaptability to methane mitigation strategies.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"16 1","pages":"162"},"PeriodicalIF":6.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145656081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01DOI: 10.1016/j.nanoms.2025.10.008
Pengcheng Liu , Guiyin Xu , Weijin Li
{"title":"Editorial for a special issue on emerging materials for carbon neutrality","authors":"Pengcheng Liu , Guiyin Xu , Weijin Li","doi":"10.1016/j.nanoms.2025.10.008","DOIUrl":"10.1016/j.nanoms.2025.10.008","url":null,"abstract":"","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 727-728"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, the demand for high-performance rechargeable lithium batteries has increased significantly, and many efforts have been made to boost the use of advanced electrode materials. Since graphene was first isolated by Novoselov et al., graphene/graphene-based materials have become an active area of research and are considered to be promising high-performance electrode materials. Graphene is a two-dimensional single-atom carbon-packed material that possesses fascinating properties, including a large surface area, remarkable electrical conductivity, extraordinary intrinsic electron mobility, high Young's modulus, superior mechanical strength, optical transmittance, catalytic performance, and stability. Therefore, graphene is considered an attractive material for rechargeable lithium-ion batteries (LIBs), lithium-sulfur batteries (LSBs), and lithium-oxygen batteries (LOBs). In this comprehensive review, we emphasise the recent progress in the controllable synthesis, functionalisation, and role of graphene in rechargeable lithium batteries. Finally, in this review, we aim to address the most promising results, benefits, challenges, critical issues, research directions, and perspectives to explain the developmental directions of graphene for batteries.
{"title":"The role of graphene in rechargeable lithium batteries: Synthesis, functionalisation, and perspectives","authors":"Asad Ali , Fengxing Liang , Jinliang Zhu , Peikang Shen","doi":"10.1016/j.nanoms.2022.07.004","DOIUrl":"10.1016/j.nanoms.2022.07.004","url":null,"abstract":"<div><div>In recent years, the demand for high-performance rechargeable lithium batteries has increased significantly, and many efforts have been made to boost the use of advanced electrode materials. Since graphene was first isolated by Novoselov et al., graphene/graphene-based materials have become an active area of research and are considered to be promising high-performance electrode materials. Graphene is a two-dimensional single-atom carbon-packed material that possesses fascinating properties, including a large surface area, remarkable electrical conductivity, extraordinary intrinsic electron mobility, high Young's modulus, superior mechanical strength, optical transmittance, catalytic performance, and stability. Therefore, graphene is considered an attractive material for rechargeable lithium-ion batteries (LIBs), lithium-sulfur batteries (LSBs), and lithium-oxygen batteries (LOBs). In this comprehensive review, we emphasise the recent progress in the controllable synthesis, functionalisation, and role of graphene in rechargeable lithium batteries. Finally, in this review, we aim to address the most promising results, benefits, challenges, critical issues, research directions, and perspectives to explain the developmental directions of graphene for batteries.</div></div>","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 818-836"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46058355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the Metal-Organic Framework (MOF) panorama, Ce(IV)-based MOFs have emerged as potential candidates for heterogeneous catalysis, not only due to their intrinsic active species but also as a support of single metal sites. As the catalytic behavior of these materials is often associated to the redox properties of cerium, a large number of spectroscopic techniques have been employed to characterize them. Unfortunately, both data acquisition and interpretation are not always straightforward and sometimes the data are not well reported and discussed, limiting their impact in the literature. In this perspective review, we critically analyse the contributions provided by different spectroscopic techniques, sometime supported by molecular modelling approaches, to unravel the nature of Ce(IV)-MOFs at any stage of their preparation and along their use (i.e., post-synthesis treatments and under reaction conditions). A concise description of major results from the recent literature allows to provide basic insights associated to the applicability and limits of most used spectroscopic approaches, showing that more robust understanding of Ce(IV)-MOFs can be achieved when a broad spectrum of techniques are used in parallel, adopting similar conditions and following good practice rules.
{"title":"Exploring Ce(IV)-MOFs redox behavior for catalysis by spectroscopies","authors":"Valeria Finelli , Sergio Rojas-Buzo , Matteo Signorile , Francesca Bonino , Silvia Bordiga","doi":"10.1016/j.nanoms.2024.11.001","DOIUrl":"10.1016/j.nanoms.2024.11.001","url":null,"abstract":"<div><div>In the Metal-Organic Framework (MOF) panorama, Ce(IV)-based MOFs have emerged as potential candidates for heterogeneous catalysis, not only due to their intrinsic active species but also as a support of single metal sites. As the catalytic behavior of these materials is often associated to the redox properties of cerium, a large number of spectroscopic techniques have been employed to characterize them. Unfortunately, both data acquisition and interpretation are not always straightforward and sometimes the data are not well reported and discussed, limiting their impact in the literature. In this perspective review, we critically analyse the contributions provided by different spectroscopic techniques, sometime supported by molecular modelling approaches, to unravel the nature of Ce(IV)-MOFs at any stage of their preparation and along their use (<em>i.e.</em>, post-synthesis treatments and under reaction conditions). A concise description of major results from the recent literature allows to provide basic insights associated to the applicability and limits of most used spectroscopic approaches, showing that more robust understanding of Ce(IV)-MOFs can be achieved when a broad spectrum of techniques are used in parallel, adopting similar conditions and following good practice rules.</div></div>","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 761-772"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01DOI: 10.1016/j.nanoms.2024.10.010
Chao Wang , Jialu Li , Flemming Besenbacher , Ren Su
Nitrogenous compounds (i.e., amines, amides, nitriles, oximes, amino acids and nitrogen-heterocycles derivatives) are important building blocks for synthetic chemistry, pharmaceuticals, and functional materials. Conventional synthetic strategies involve the use of toxic organic nitrogenous precursors or expensive heterogeneous catalysts under elevated temperatures and pressurized oxygen. Heterogeneous electrocatalysis can initiate the activation of inorganic N sources (i.e., NH3 and NOx−) under ambient reactions in liquid phase by applying a small bias, thus allowing the synthesis of value−added nitrogenous compounds from carbonyls, alkenes, keto acids, and even carbon dioxide in a sustainable manner without the use of oxidants/reductants. This review outlines recent developments in electrosynthesis of nitrogenous compounds using inorganic N sources, focusing on reaction mechanisms understanding, the design and optimization of efficient electrocatalysts, and the advances in cell configurations for various C‒N coupling reactions. The limitations and challenges in applications are also discussed.
{"title":"Heterogeneous electrocatalytic synthesis of nitrogenous organic compounds using inorganic nitrogen sources","authors":"Chao Wang , Jialu Li , Flemming Besenbacher , Ren Su","doi":"10.1016/j.nanoms.2024.10.010","DOIUrl":"10.1016/j.nanoms.2024.10.010","url":null,"abstract":"<div><div>Nitrogenous compounds (<em>i.e.,</em> amines, amides, nitriles, oximes, amino acids and nitrogen-heterocycles derivatives) are important building blocks for synthetic chemistry, pharmaceuticals, and functional materials. Conventional synthetic strategies involve the use of toxic organic nitrogenous precursors or expensive heterogeneous catalysts under elevated temperatures and pressurized oxygen. Heterogeneous electrocatalysis can initiate the activation of inorganic N sources (<em>i.e.</em>, NH<sub>3</sub> and NO<sub><em>x</em></sub><sup>−</sup>) under ambient reactions in liquid phase by applying a small bias, thus allowing the synthesis of value−added nitrogenous compounds from carbonyls, alkenes, keto acids, and even carbon dioxide in a sustainable manner without the use of oxidants/reductants. This review outlines recent developments in electrosynthesis of nitrogenous compounds using inorganic N sources, focusing on reaction mechanisms understanding, the design and optimization of efficient electrocatalysts, and the advances in cell configurations for various C‒N coupling reactions. The limitations and challenges in applications are also discussed.</div></div>","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 773-789"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01DOI: 10.1016/j.nanoms.2024.07.003
Jie Zhang , Kongjun Zhu , Zhihan Kong , Dingwei Ji , Penghua Liang , Jing Wang , Kang Yan , Jinsong Liu
To accelerate the development of lithium-ion batteries (LIBs), researchers should urgently exploit next-generation electrodes with high specific capacity, long cycle stability, and excellent rate performance, such as TMOs, silicon-based materials, and alloys. Among all the modification measures, hierarchical micro-nano structure and yolk–shell structure are considered suitable and effective ways to improve the electrochemical performance of those novel materials. Herein, a facile glucose-assisted solvothermal method combined with heat treatment was implemented to synthesize hierarchical micro-nano yolk–shell V2O3. The special-structured material exhibited higher specific capacity, better structure stability, and faster electrochemical kinetics compared with nanosheet-structured and micro-nano-cluster-structured V2O3. When used as an anode for LIB, mnYS-V2O3 delivered high specific capacity of 650.1 mA h g−1 after over 500 cycles at a current density of 100 mA g−1, with a retention of 93.4 %. Moreover, the morphology evolution mechanism of micro-nano structure and yolk–shell structure was investigated in this work, which is beneficial to the design of other mnYS-structured TMOs.
为了加速锂离子电池（LIBs）的发展，研究人员迫切需要开发具有高比容量、长周期稳定性和优异倍率性能的下一代电极，如TMOs、硅基材料和合金。在这些改性措施中，分层微纳结构和蛋黄壳结构被认为是改善这些新型材料电化学性能的合适和有效的方法。本研究采用葡萄糖辅助溶剂热结合热处理的方法合成了层状微纳米蛋黄壳V2O3。与纳米片状和微纳米团簇结构的V2O3相比，该特殊结构材料具有更高的比容量、更好的结构稳定性和更快的电化学动力学。作为锂离子电池的阳极，mys - v2o3在100 mA g - 1的电流密度下，经过500次循环后，其比容量高达650.1 mA h g - 1，保留率为93.4%。此外，本文还研究了微纳结构和蛋黄壳结构的形态演化机制，为其他mys结构TMOs的设计提供了理论依据。
{"title":"Glucose-assisted solvothermal synthesis of hierarchical micro-nano yolk–shell V2O3 microspheres as an anode material for lithium-ion batteries","authors":"Jie Zhang , Kongjun Zhu , Zhihan Kong , Dingwei Ji , Penghua Liang , Jing Wang , Kang Yan , Jinsong Liu","doi":"10.1016/j.nanoms.2024.07.003","DOIUrl":"10.1016/j.nanoms.2024.07.003","url":null,"abstract":"<div><div>To accelerate the development of lithium-ion batteries (LIBs), researchers should urgently exploit next-generation electrodes with high specific capacity, long cycle stability, and excellent rate performance, such as TMOs, silicon-based materials, and alloys. Among all the modification measures, hierarchical micro-nano structure and yolk–shell structure are considered suitable and effective ways to improve the electrochemical performance of those novel materials. Herein, a facile glucose-assisted solvothermal method combined with heat treatment was implemented to synthesize hierarchical micro-nano yolk–shell V<sub>2</sub>O<sub>3</sub>. The special-structured material exhibited higher specific capacity, better structure stability, and faster electrochemical kinetics compared with nanosheet-structured and micro-nano-cluster-structured V<sub>2</sub>O<sub>3</sub>. When used as an anode for LIB, mnYS-V<sub>2</sub>O<sub>3</sub> delivered high specific capacity of 650.1 mA h g<sup>−1</sup> after over 500 cycles at a current density of 100 mA g<sup>−1</sup>, with a retention of 93.4 %. Moreover, the morphology evolution mechanism of micro-nano structure and yolk–shell structure was investigated in this work, which is beneficial to the design of other mnYS-structured TMOs.</div></div>","PeriodicalId":33573,"journal":{"name":"Nano Materials Science","volume":"7 6","pages":"Pages 837-846"},"PeriodicalIF":17.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-29DOI: 10.1186/s40104-025-01286-7
Fengyu Xiang, Heng Yang, Xiangqi Fan, Dayan Tan, Bing Huang, Bing Yu, Jun He, Yuheng Luo, Junqiu Luo, Hui Yan, Junning Pu, Jianping Wang, Quyuan Wang, Huifen Wang, John Kyaw Htoo, Santa Maria Mendoza, Guiling Yan, Xiangbing Mao
Background: As probiotics, Bacillus strains may regulate some physiological functions in animals. This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage induced by rotavirus (RV) infection in piglets. Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet (n = 16) and the diet containing 109 colony-forming unit Bacillus spores/kg (n = 8). On d 8, 8 piglets fed with the diet supplemented with Bacillus-based probiotic and 8 piglets fed with basal diet were orally infused with RV, while the residue piglets had oral gavage of sterile essential medium. The trial duration was 12 d.
Results: RV challenge induced diarrhea, significantly destroyed the morphology of jejunal mucosa (P < 0.05), significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa (P < 0.05), significantly impaired antioxidant capacity (including malondialdehyde level, total antioxidant capacity and catalase activity), immunity (such as interleukin 2, interleukin 4 and secreted immunoglobulin A levels), mucins and the mRNA expression of tight-junction-related (such as Zonula occludens 1, occludin) and apoptotic-related (including B-cell lymphoma/leukaemia-2-associated X protein, B cell lymphoma/leukaemia-2, cysteinyl aspartate specific proteinases) genes of jejunal mucosa (P < 0.05), and, to some extents, affected the bacteria community structure and abundance of ileal digesta in piglets. However, Bacillus-based probiotic administration could significantly attenuate the negative effects of RV infection on gut health of piglets (P < 0.05).
Conclusions: These findings suggested that supplementing Bacillus-based probiotic in the diet could decrease diarrhea rate, and improve gut health in weaned piglets, which was associated with regulating intestinal antioxidant capacity, apoptosis, and microbiota.
{"title":"Dietary supplementation with Bacillus-based probiotic improves gut health in the weaned piglets challenged by rotavirus.","authors":"Fengyu Xiang, Heng Yang, Xiangqi Fan, Dayan Tan, Bing Huang, Bing Yu, Jun He, Yuheng Luo, Junqiu Luo, Hui Yan, Junning Pu, Jianping Wang, Quyuan Wang, Huifen Wang, John Kyaw Htoo, Santa Maria Mendoza, Guiling Yan, Xiangbing Mao","doi":"10.1186/s40104-025-01286-7","DOIUrl":"10.1186/s40104-025-01286-7","url":null,"abstract":"<p><strong>Background: </strong>As probiotics, Bacillus strains may regulate some physiological functions in animals. This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage induced by rotavirus (RV) infection in piglets. Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet (n = 16) and the diet containing 10<sup>9</sup> colony-forming unit Bacillus spores/kg (n = 8). On d 8, 8 piglets fed with the diet supplemented with Bacillus-based probiotic and 8 piglets fed with basal diet were orally infused with RV, while the residue piglets had oral gavage of sterile essential medium. The trial duration was 12 d.</p><p><strong>Results: </strong>RV challenge induced diarrhea, significantly destroyed the morphology of jejunal mucosa (P < 0.05), significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa (P < 0.05), significantly impaired antioxidant capacity (including malondialdehyde level, total antioxidant capacity and catalase activity), immunity (such as interleukin 2, interleukin 4 and secreted immunoglobulin A levels), mucins and the mRNA expression of tight-junction-related (such as Zonula occludens 1, occludin) and apoptotic-related (including B-cell lymphoma/leukaemia-2-associated X protein, B cell lymphoma/leukaemia-2, cysteinyl aspartate specific proteinases) genes of jejunal mucosa (P < 0.05), and, to some extents, affected the bacteria community structure and abundance of ileal digesta in piglets. However, Bacillus-based probiotic administration could significantly attenuate the negative effects of RV infection on gut health of piglets (P < 0.05).</p><p><strong>Conclusions: </strong>These findings suggested that supplementing Bacillus-based probiotic in the diet could decrease diarrhea rate, and improve gut health in weaned piglets, which was associated with regulating intestinal antioxidant capacity, apoptosis, and microbiota.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"16 1","pages":"161"},"PeriodicalIF":6.5,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12664204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Inflammatory bowel disease causes intestinal structural damage, impairs gut function, hinders animal growth and development, and reduces farming efficiency. Previous studies demonstrated that lactate alleviates dextran sulfate sodium (DSS)-induced inflammation and mitigates weight loss by enhancing intestinal barrier functions. However, the mechanisms underlying lactate-mediated protection of the intestinal epithelial barrier remain unclear. This study aimed to explore the protective effect of lactate on intestinal barrier damage in colitis piglets and the possible underlying mechanisms through in vivo and in vitro experiments.
Methods: A total of 60 21-day-old weaned female piglets were randomly assigned into three groups based on weight: the control group (basal diet with physiological saline gavage), the DSS group (basal diet with 5% DSS gavage), and the DSS + LA group (2% lactate diet with 5% DSS gavage). There were 10 replicates per treatment, with 2 piglets per replicate. Jejunal morphology was assessed via hematoxylin and eosin staining, while Western blotting quantified the protein levels of proliferation markers, including cluster of differentiation 24 (CD24), cyclin D1, and wingless/integrated (Wnt)/β-catenin signaling components. In vitro, 0.08% DSS and 2-32 mmol/L sodium lactate-treated intestinal porcine epithelial cell line-J2 (IPEC-J2) cells (n = 4) were assessed for viability (Cell Counting Kit-8 assay), apoptosis (flow cytometry), and proliferation parameters, including cell cycle analysis and Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5+) stem cell quantification.
Results: In vivo, DSS administration induced jejunal villus shortening (P < 0.05), downregulated protein levels of CD24, cyclin D1, casein kinase 1 (CK1), and dishevelled-2 (DVL2) (P < 0.05). In vitro, DSS promoted apoptosis, inhibited proliferation, diminished the Lgr5+ cell populations (P < 0.05), and reduced S-phase cell proportions (P < 0.05). Conversely, lactate supplementation ameliorated DSS-induced villus atrophy (P < 0.05), restored CD24, cyclin D1, CK1, and DVL2 protein levels (P < 0.05). Furthermore, in vitro, sodium lactate attenuated DSS-induced apoptosis (P < 0.05), enhanced IPEC-J2 proliferation (P < 0.05), expanded Lgr5+ cells (P < 0.05), and increased S-phase progression (P < 0.05).
Conclusions: In summary, lactate ameliorated intestinal barrier damage in DSS-induced colitis by activating the Wnt/β-catenin pathway and restoring the balance between epithelial cell proliferation and apoptosis. This study provides novel mechanistic evidence supporting lactate's therapeutic potential for IBD management.
{"title":"Lactate alleviates intestinal barrier injury in weaned piglets via activation of the Wnt/β-catenin pathway and promotion of intestinal epithelial cell proliferation.","authors":"Mingyu Wang, Yifan Chen, Jiaojiao Chen, Aimin Wu, Daiwen Chen, Bing Yu, Jun He, Jie Yu, Xiangbing Mao, Zhiqing Huang, Yuheng Luo, Junqiu Luo, Ping Zheng","doi":"10.1186/s40104-025-01290-x","DOIUrl":"10.1186/s40104-025-01290-x","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory bowel disease causes intestinal structural damage, impairs gut function, hinders animal growth and development, and reduces farming efficiency. Previous studies demonstrated that lactate alleviates dextran sulfate sodium (DSS)-induced inflammation and mitigates weight loss by enhancing intestinal barrier functions. However, the mechanisms underlying lactate-mediated protection of the intestinal epithelial barrier remain unclear. This study aimed to explore the protective effect of lactate on intestinal barrier damage in colitis piglets and the possible underlying mechanisms through in vivo and in vitro experiments.</p><p><strong>Methods: </strong>A total of 60 21-day-old weaned female piglets were randomly assigned into three groups based on weight: the control group (basal diet with physiological saline gavage), the DSS group (basal diet with 5% DSS gavage), and the DSS + LA group (2% lactate diet with 5% DSS gavage). There were 10 replicates per treatment, with 2 piglets per replicate. Jejunal morphology was assessed via hematoxylin and eosin staining, while Western blotting quantified the protein levels of proliferation markers, including cluster of differentiation 24 (CD24), cyclin D1, and wingless/integrated (Wnt)/β-catenin signaling components. In vitro, 0.08% DSS and 2-32 mmol/L sodium lactate-treated intestinal porcine epithelial cell line-J2 (IPEC-J2) cells (n = 4) were assessed for viability (Cell Counting Kit-8 assay), apoptosis (flow cytometry), and proliferation parameters, including cell cycle analysis and Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5<sup>+</sup>) stem cell quantification.</p><p><strong>Results: </strong>In vivo, DSS administration induced jejunal villus shortening (P < 0.05), downregulated protein levels of CD24, cyclin D1, casein kinase 1 (CK1), and dishevelled-2 (DVL2) (P < 0.05). In vitro, DSS promoted apoptosis, inhibited proliferation, diminished the Lgr5<sup>+</sup> cell populations (P < 0.05), and reduced S-phase cell proportions (P < 0.05). Conversely, lactate supplementation ameliorated DSS-induced villus atrophy (P < 0.05), restored CD24, cyclin D1, CK1, and DVL2 protein levels (P < 0.05). Furthermore, in vitro, sodium lactate attenuated DSS-induced apoptosis (P < 0.05), enhanced IPEC-J2 proliferation (P < 0.05), expanded Lgr5<sup>+</sup> cells (P < 0.05), and increased S-phase progression (P < 0.05).</p><p><strong>Conclusions: </strong>In summary, lactate ameliorated intestinal barrier damage in DSS-induced colitis by activating the Wnt/β-catenin pathway and restoring the balance between epithelial cell proliferation and apoptosis. This study provides novel mechanistic evidence supporting lactate's therapeutic potential for IBD management.</p>","PeriodicalId":64067,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"16 1","pages":"160"},"PeriodicalIF":6.5,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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