化学•材料最新文献

{"title":"Hydrogel molecularly imprinted polymer–based electrochemical sensor on gold-modified screen-printed carbon electrodes for stable and selective HbA1c detection","authors":"Launa Silky Karenindra Rokhmat ,&nbsp;Irkham ,&nbsp;Putra Rafli Ramdani ,&nbsp;Serly Zuliska ,&nbsp;Wulan Khaerani ,&nbsp;Dika Apriliana Wulandari ,&nbsp;Adisyahputra ,&nbsp;Santhy Wyantuti ,&nbsp;Iman Permana Maksum ,&nbsp;Yasuaki Einaga ,&nbsp;Genki Ogata ,&nbsp;Yeni Wahyuni Hartati","doi":"10.1016/j.bios.2026.118466","DOIUrl":"10.1016/j.bios.2026.118466","url":null,"abstract":"<div><div>Glycated hemoglobin (HbA1c) is a key biomarker for long-term glycemic control and diabetes diagnosis. Common methods such as chromatography and immunoassays are accurate but require costly instrumentation, limiting their use in point-of-care settings. Electrochemical sensors offer a portable and sensitive alternative. This study integrates computational modeling with hydrogel-based molecularly imprinted polymer (MIP) engineering to achieve selective HbA1c recognition. Molecular docking coupled with molecular dynamics simulations guided the rational design, identifying optimal binding sites and confirming the structural stability of the monomer–template complex. A hydrogel MIP sensor was fabricated on a gold-modified screen-printed carbon electrode using 2-acrylamido-2-methyl-1-propanesulfonic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker. Successful polymer formation and template removal were confirmed by FTIR and XPS, while SEM verified uniform deposition of gold particles and MIP layers. Electrochemical characterization was performed using K₃[Fe(CN)₆]as the redox probe. The developed SPCE/Au/MIPs sensor exhibited a wide linear range 10–10⁵ ng/mL and LOD 1.13 ng/mL. A strong imprinting factor (IF 4.96) and high selectivity (98.7 %) confirmed effective molecular recognition, while long-term stability was demonstrated by stable performance over 120 days at room temperature with 94 % signal retention. Real sample measurements showed 95–112 % recovery compared to NGSP-standardized HPLC, with good reproducibility, validating its practical applicability. Overall, hydrogel MIP platform provides a robust, sensitive, and durable approach for HbA1c monitoring, supporting good health and well-being through accessible diabetes diagnostics.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118466"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146098707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-initiated exponential amplification on fluorescently-barcoded microspheres for deep learning-assisted multiplexed HPV detection","authors":"Tingting Bai ,&nbsp;Xiaojun Qu ,&nbsp;Jinshun Pan ,&nbsp;Yuzhen Tang ,&nbsp;Luhai Wang ,&nbsp;Ping Zhou ,&nbsp;Zhenzhen Hu ,&nbsp;Zhirui Guo ,&nbsp;Yefei Zhu ,&nbsp;Yu Zhang","doi":"10.1016/j.bios.2026.118488","DOIUrl":"10.1016/j.bios.2026.118488","url":null,"abstract":"<div><div>Rapid, low-cost, and multiplexed nucleic acid testing is essential for human health but remains challenging. Although CRISPR-Cas systems offer high specificity, their integration into Multiplexed platforms suitable for near-patient testing has been limited. Here, we introduce a biosensing platform that combines CRISPR-initiated enzymatic amplification with quantum-dot encoded microbeads and deep-learning based image analysis for Multiplexed detection of human papillomavirus (HPV) DNA. The high specificity of CRISPR/Cas9 first triggers an exponential amplification. The products are then specifically captured on the microbead surface for localized fluorescent readout. A custom deep-learning algorithm automatically quantifies the bead fluorescence, enabling robust and automated analysis. The integrated approach achieves simultaneous detection of HPV16, HPV18, and HPV33 with detection limits as low as 0.2 pM. By using recognition-triggered amplification and a simple deep-learning assisted fluorescence readout, the workflow is significantly simplified. The platform establishes a universal and practical strategy for molecular diagnostics, demonstrating strong potential for near-patient testing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118488"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic catalysis of triethylamine by Pd nanocubes/Au nanoclusters heterostructure: An enhanced Au nanoclusters-triethylamine electrochemiluminescence system for As3+ aptasensing","authors":"Cheng Xu ,&nbsp;Jiali Huang ,&nbsp;Jiayi Liu ,&nbsp;Yinhui Yi ,&nbsp;Yao Zhu ,&nbsp;Deke Xing ,&nbsp;Libo Li ,&nbsp;Tianyan You","doi":"10.1016/j.bios.2026.118483","DOIUrl":"10.1016/j.bios.2026.118483","url":null,"abstract":"<div><div>Enhancing the electrochemiluminescence (ECL) of Au nanoclusters (AuNCs)-based system is a crucial approach for broadening their applications. Unlike traditional strategies that relied solely on coreaction accelerators to promote coreactant oxidation, this work innovatively adopted a “luminophore-coreaction accelerator” synergistic catalysis strategy to enhance the ECL of the AuNCs-Triethylamine (TEA) system. Specifically, Pd nanocubes (PdNCs), serving as a coreaction accelerator, formed a heterostructure with AuNCs on the working electrode surface. This structural design enhanced the ECL signal of the AuNCs-TEA system via two mechanisms. On one hand, PdNCs acted as efficient “electron traps” to capture electrons injected from TEA into the lowest unoccupied molecular orbital (LUMO) of AuNCs, thus significantly promoting the oxidation of TEA. On the other hand, the incorporation of PdNCs effectively improved the electrochemically active surface area of AuNCs, thus providing more active sites to catalyze the oxidation of TEA. Together, these two mechanisms accelerated the generation of TEA free radicals (TEA^•) and the subsequent excited state of AuNCs (AuNCs*), thereby enhancing the ECL of the system. As an application, the developed ECL aptasensor exhibited favorable analytical performance for As³⁺. This study has opened up a new way to improve the ECL properties of metal nanoclusters.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118483"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-mechanism-driven dual-mode array based on a single PFC-1/QD probe enables AI-assisted on-site identification of biogenic amines and real-time food freshness monitoring","authors":"Lanqing Yang ,&nbsp;Shilin Li ,&nbsp;Rong Guo ,&nbsp;Baoqing Bai ,&nbsp;Ying Zhang ,&nbsp;Wenyan Yan ,&nbsp;Shan Liu ,&nbsp;Shasha Zhao ,&nbsp;Wenbo Lu ,&nbsp;Yukun Yang","doi":"10.1016/j.bios.2026.118501","DOIUrl":"10.1016/j.bios.2026.118501","url":null,"abstract":"<div><div>Biogenic amines (BAs) serve as critical indicators of food spoilage, necessitating portable and real-time monitoring approaches to ensure food safety. To this end, we developed a P-QDot-based fluorescence/colorimetric (FL/CL) dual-mode sensor array integrated with deep learning and smartphone imaging for the classification and quantification of five BAs and the assessment of food freshness. The P-QDot probe was prepared by physically mixing the hydrogen-bonded organic framework PFC-1 with glutathione-capped quantum dots (QD) in aqueous solution. The fluorescence response was governed by a synergistic effect involving aggregation-induced emission (AIE), photoinduced electron transfer (PET), and the inner filter effect (IFE), which were activated by hydrogen bonding between the probe and BAs. Concurrently, a new UV absorption band was generated by P-QDot under the alkaline conditions induced by the BAs, leading to the colorimetric response. Leveraging this dual-mode response mechanism, the P-QDot-based FL/CL sensing array facilitated rapid (&lt;30 s), sensitive, and simultaneous qualitative discrimination and quantitative detection of BAs. Furthermore, a smartphone platform integrated with the YOLOv12 algorithm was established, enabling on-site BAs classification. The practical application of this system was demonstrated by real-time monitoring of shrimp spoilage during storage at 4 °C and 25 °C, where visually recognizable fluorescence color changes and colorimetric signals accurately reflected the degree of spoilage. Additionally, a logic gate device was engineered to directly translate the complex sensor signals into three intuitive freshness levels (fresh, acceptable freshness, and spoiled). This work presented a powerful strategy for food freshness assessment, successfully merging intricate material design with portable intelligence.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118501"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating efficient signal amplification and magnetic separation into a homogeneous electrochemical strategy: Achieving ultra-low background simultaneous detection of aflatoxin B1 and ochratoxin A","authors":"Chunyuan Tang ,&nbsp;Bingzheng Yuan ,&nbsp;Libo Li ,&nbsp;Lijun Luo ,&nbsp;Tianyan You","doi":"10.1016/j.bios.2026.118465","DOIUrl":"10.1016/j.bios.2026.118465","url":null,"abstract":"<div><div>Coexisting aflatoxin B1 (AFB1) and ochratoxin A (OTA) can produce synergetic toxic effects, particularly carcinogenicity, highlighting the importance of their simultaneous detection. Although homogeneous strategies can avoid cross-interference on the electrode surface during multi-target detection, the complexity of the signal amplification process and background interference remains a challenge. This study integrates an efficient signal amplification strategy and magnetic separation technology into a homogeneous electrochemical strategy for the simultaneous detection of AFB1 and OTA. First, taking advantage of the adsorption of Zr-based metal-organic framework (UiO-66) for electroactive dyes, the aptamer was combined with it via the stirring method, followed by enrichment of neutral red (NR)/methylene blue (MB) to prepare a signal amplification probe. Then, the probe capture was achieved through double-strand hybridization by using complementary DNA (cDNA)-functionalized Fe₃O₄@PtNPs. Once the target was present, the high specificity of the aptamer forced the release of the signal probe. Meanwhile, the external magnet can rapidly remove the background-interfering species that are independent of the target. By ingeniously integrating the advantages of low background and an efficient signal amplification strategy, this method successfully achieved highly sensitive simultaneous detection of AFB1 and OTA. The detection ranges for AFB1 and OTA were 1 pg/mL to 100 ng/mL and 500 fg/mL to 100 ng/mL, with detection limits as low as 0.51 pg/mL and 0.42 pg/mL, respectively. Overall, this strategy represents a satisfactory exploration towards achieving efficient and sensitive strategies for detecting numerous mycotoxins.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118465"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active-site rich nanostructure with dual-enhanced electron transfer for ultra-sensitive electrochemiluminescence detection of DNA methylation in colon cancer","authors":"Xiao Tian ,&nbsp;Yuting Hou ,&nbsp;Liang Luan ,&nbsp;Lingzhi Ye ,&nbsp;Junjie Yuan ,&nbsp;Yating Zhang ,&nbsp;Mingliang Li ,&nbsp;Xiangmeng Qu ,&nbsp;Nan Wan","doi":"10.1016/j.bios.2026.118470","DOIUrl":"10.1016/j.bios.2026.118470","url":null,"abstract":"<div><div>Traditional DNA methylation assays for colon cancer still face challenges of insufficient sensitivity, limited specificity, and low cost-efficiency in clinical samples. Herein, we develop an electrochemiluminescence biosensor based on a dual-enhanced electron transfer mechanism using DNA tetrahedral nanostructure probes for the ultrasensitive detection of methylated DNA related to colon cancer. The DNA tetrahedral structure probe overcomes the shortcomings of poor adaptability caused by the low binding efficiency and high spatial steric hindrance in complex clinical samples. The electrochemiluminescence biosensor uses active-site-rich TiO₂-Ti₃C₂ MXenes@AgNPs hybrid nanocomposites as the electro-active substrate. These nanocomposites exhibited accelerated electron transfer and shortened the reaction paths due to the intrinsic photoelectric activity of TiO₂ and the surface plasmon resonance (SPR) effect between AgNPs and TiO₂. Furthermore, the three-dimensional (3D) self-assembled AuNPs-ABEI nanoluminous spheres substantially improved the luminescence intensity by concentrating more AuNPs to accelerate electron transfer while immobilizing additional ABEI molecules. The dual-enhanced electron transfer strategy significantly accelerated the kinetics of interfacial electron transfer. Metal nanoparticles (such as AgNP, AuNP, and TiO₂) generate SPR-induced local electric fields under electrochemical excitation. The electric fields enhance the electron transfer and amplify the electrochemiluminescence intensity, thereby achieving ultra-sensitive detection of the methylated Septin9 gene with a detection limit as low as 8.2 aM. Clinical sample validation using clinical serum samples from healthy controls (n = 100) and colon cancer patients (n = 100) has shown that the biosensor achieves comparable performance to the gold standard method in terms of both sensitivity and specificity, demonstrating equivalent detection efficacy to pyrosequencing.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"300 ","pages":"Article 118470"},"PeriodicalIF":10.5,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared-autosampler parallel LC–MS/MS platform for high-throughput multi-analyte quantification in complex matrices","authors":"Shanshan Cai ,&nbsp;Qisheng Zhong ,&nbsp;Jiaqi Liu ,&nbsp;Lisong Chen ,&nbsp;Lingling Shen ,&nbsp;Hongyuan Hao ,&nbsp;Ting Zhou","doi":"10.1016/j.chroma.2026.466885","DOIUrl":"10.1016/j.chroma.2026.466885","url":null,"abstract":"<div><div>Modern analytical tasks increasingly require the simultaneous quantification of large numbers of chemically diverse analytes in complex matrices, which remains challenging for a single LC–MS method. Here, we developed a shared-autosampler parallel LC–MS/MS strategy that enables two fully independent chromatographic methods to be executed sequentially within a single analytical cycle while generating one unified chromatogram-mass spectrum. The system employed two LC pump modules and two columns to form two independent LC pathways, which shared one autosampler and one MS detector for selective MRM acquisition and subsequent signal integration. Integration was performed exclusively at the MS detection and data-processing level, without inter-dimensional coupling or analyte transfer between columns. This avoided the mobile-phase compatibility and dead-volume limitations associated with 2D-LC, enabled modular recombination of chromatographic and MS conditions, and improved analytical throughput without additional MS detector or autosampler hardware. The performance of the strategy was demonstrated in two applications. An acidic/basic dual-method configuration enabled quantitative analysis of 394 emerging contaminants from 14 chemical classes in human serum within 28 min, exhibiting good linearity, limits of quantification not exceeding 0.20 ng/mL for &gt;90 % of analytes, and peak-area RSDs generally below 10 %. A HILIC/RP parallel-column combination achieved simultaneous determination of 27 hypoglycemic agents spanning a log <em>P</em> range of −6.8 to 5.9 in a single 20 min run, reducing analysis time by &gt;50 % compared with standard methods, with good precision and recoveries in spiked food samples. Overall, the shared-autosampler parallel LC–MS/MS strategy provided a flexible and efficient platform for high-throughput multi-analyte quantification in complex matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466885"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics and in-house database analysis reveal differences between elephantopus scaber L. and elephantopus tomentosus L.","authors":"Chang-Sheng Gao ,&nbsp;Zhi-Kang Duan ,&nbsp;Mei-Ya Lian,&nbsp;Run-Ze Yu,&nbsp;Ming Bai,&nbsp;Shu-Hui Dong,&nbsp;Shao-Jiang Song","doi":"10.1016/j.chroma.2026.466881","DOIUrl":"10.1016/j.chroma.2026.466881","url":null,"abstract":"<div><div>In China, <em>Elephantopus scaber</em> L. and <em>Elephantopus tomentosus</em> L.<em>,</em> two traditional Chinese medicinal herbs commonly known as \"Ku-di-dan\", are widely used. Moreover, they have demonstrated anti-hepatoma effects and hepatoprotective properties in experimental studies. Nonetheless, according to existing literature, <em>E. scaber</em> and <em>E. tomentosus</em> encompass compounds with similar but notably different chemical structures. Therefore, investigating the differences in secondary metabolites between <em>E. scaber</em> and <em>E. tomentosus</em> and elucidating their distinct anti-hepatocellular carcinoma activities is of great importance. This study employed an integrated approach combining untargeted metabolomics with bioactivity assays to effectively differentiate between the congeneric plant species <em>E. scaber</em> and <em>E. tomentosus</em>. To improve the accuracy of characterizing and identifying key metabolites, an in-house database and data screening methods were employed. The research findings revealed that thirty major secondary metabolites were detected in <em>E. tomentosus</em> and <em>E. scaber</em> extracts through untargeted metabolomics and data screening. Subsequently, eight significant differential metabolites were identified through multivariate statistical analyses. Thereafter, twelve compounds were isolated from these two plants and evaluated for their anti-hepatocellular activity, revealing that compounds <strong>5</strong>—<strong>8</strong> exhibited significant inhibitory effects on HepG2 and Hep3B cells. Consistent results were also observed in the tests conducted on the crude extracts of both <em>E. scaber</em> and <em>E. tomentosus</em>. This study demonstrates that <em>E. scaber</em> and <em>E. tomentosus</em> exhibit distinct secondary metabolite profiles and differential anti-hepatocellular carcinoma activities.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466881"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computer vision–based augmented visualisation for coffee origins identitation using comprehensive two-dimensional gas chromatography","authors":"Giorgio Felizzato ,&nbsp;Eloisa Bagnulo ,&nbsp;Giulia Tapparo ,&nbsp;Giorgia Botta ,&nbsp;Qingping Tao ,&nbsp;Stephen E. Reichenbach ,&nbsp;Chiara Cordero ,&nbsp;Luciano Navarini ,&nbsp;Erica Liberto ,&nbsp;Andrea Caratti","doi":"10.1016/j.chroma.2026.466836","DOIUrl":"10.1016/j.chroma.2026.466836","url":null,"abstract":"<div><div>Coffee is a highly complex and variable matrix, with volatile profiles shaped by multiple factors including botanical origin, climatic and soil conditions, post-harvest treatments, and roasting parameters. This variability generates complex chemical patterns, encompassing hundreds of volatile compounds from diverse chemical classes including pyrazines, furans, aldehydes, ketones, and terpenes. The resulting chemical dimensionality poses significant analytical challenges, making the accurate identification of characteristic compounds and reliable discrimination of coffee origins particularly difficult.</div><div>In this study, we applied comprehensive two-dimensional gas chromatography (GC×GC) coupled with computer vision (CV) to address these challenges. The workflow begins with untargeted fingerprinting, capturing all detectable compounds in a feature template. Multiple sample chromatograms are then combined into composite class images, representing the typical chemical features of each origin while minimising individual variability, which enables rapid pairwise comparison of different origins.</div><div>CV-based pairwise comparisons highlight differential peaks, which are integrated into a targeted template for subsequent peak extraction. Multivariate analyses then identify the key discriminant compounds driving origin differentiation. Post-processing strategies, such as ion-specific intensity mapping, further enhance interpretability, enabling visualisation of compositional differences across key chemical families. Overall, this GC×GC–CV workflow provides a robust, rapid, and visually intuitive platform for comprehensive chemical characterisation and origin classification of coffee, integrating untargeted and targeted analyses in a single framework.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466836"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in capillary gel electrophoresis for detecting biomacromolecules","authors":"Wen-Jing Liu ,&nbsp;Ying Zhang ,&nbsp;Qiu-Yang Li ,&nbsp;Rui-Ting Li ,&nbsp;Long Zhao ,&nbsp;Tharcisse Gatera ,&nbsp;Qu-Huan Ma ,&nbsp;Jun-Li Yang ,&nbsp;Xiao-Feng Shi","doi":"10.1016/j.chroma.2026.466850","DOIUrl":"10.1016/j.chroma.2026.466850","url":null,"abstract":"<div><div>Capillary gel electrophoresis (CGE) has been employed for the separation of biomacromolecules for over thirty years. Thanks to advances in gel materials and the ongoing development of electrophoresis instruments, the CGE method has now become one of the recognized gold standards for routine separation and analysis of biomacromolecules. This is due to its superior separation capabilities and the high robustness and reliability of its results. This paper reviews the research progress and applications of CGE in the separation and analysis of biomacromolecules, including proteins, nucleic acids, and polysaccharides, between 2015 and 2025, and offers a prospect on the future development of this technology.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466850"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}