Fungal Interactions最新文献

英文中文

Modulating Aspergillus fumigatus biofilm formation: Antifungal-induced alterations in conidium-abiotic surface interactions 调节曲霉菌生物膜的形成：抗真菌诱导的分生孢子-生物表面相互作用的改变

Fungal Interactions

Pub Date : 2024-02-07 DOI: 10.1016/j.funint.2024.100002

Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz

Biofilm prevention on surfaces supporting microbial growth is an alternative strategy to manipulating microbial cells. This study focuses on Aspergillus fumigatus, a prominent airborne fungal pathogen. We exposed glass, acrylic, high-density polyethylene (HDPE), Nylon 6, polytetrafluoroethylene (PTFE), silicone, and unplasticized polyvinyl chloride (uPVC) surfaces to antifungal agents (triclosan, liposomal amphotericin-B (L-AMB), tyrosol, and farnesol) to study A. fumigatus conidium-abiotic surfaces interactions.

The total protein concentrations of A. fumigatus mycelia were quantified after growth in both a broth medium and on agar, subsequent to treatment with the agents. The hydrophobicity of chosen surfaces and the fungus was assessed using the contact angle and the microbial adhesion to hydrocarbons (MATH) assays, respectively, when subjected to antifungal agents. Moreover, A. fumigatus biofilms on uPVC and PTFE were evaluated through transmission flow-cell culture and optical microscopy.

Hydrophobic surfaces (PTFE and silicone) impregnated with farnesol transformed into hydrophilic. Conversely, L-AMB altered the surface properties of uPVC from hydrophilic to hydrophobic, potentially as a result of L-AMB's interaction with the TiO₂ component in uPVC. Considering the effect of antifungals on conidia, A. fumigatus conidia surfaces exhibited a shift from hydrophobic to hydrophilic characteristics under the influence of these agents.

在支持微生物生长的表面防止生物膜是操纵微生物细胞的另一种策略。本研究的重点是曲霉菌，一种主要的空气传播真菌病原体。我们将玻璃、丙烯酸树脂、高密度聚乙烯（HDPE）、尼龙 6、聚四氟乙烯（PTFE）、硅树脂和未增塑聚氯乙烯（uPVC）表面暴露于抗真菌剂（三氯生、脂质体两性霉素-B（L-AMB）、酪醇和法尼醇）中，研究曲霉菌分生孢子的生长。烟曲霉菌丝体在肉汤培养基和琼脂培养基中生长后，经抗真菌剂处理，其总蛋白质浓度被量化。在使用抗真菌剂后，分别使用接触角和微生物对碳氢化合物（MATH）的粘附力测定法评估了所选表面和真菌的疏水性。此外，还通过透射流动细胞培养和光学显微镜评估了 UPVC 和 PTFE 上的烟曲霉生物膜。相反，L-AMB 会改变 uPVC 的表面性质，使其从亲水性变为疏水性，这可能是 L-AMB 与 uPVC 中的 TiO2 成分相互作用的结果。考虑到抗真菌剂对分生孢子的影响，烟曲霉分生孢子表面在这些制剂的影响下表现出从疏水到亲水的转变。

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引用次数: 0

Fungal Interactions

Pub Date : 2024-02-01 DOI: 10.1016/j.funint.2024.100002

Roya Tamimi, G. Kyazze, T. Keshavarz

引用次数: 0

Mycoremediation of n-alkanes under aerobic conditions – A review 有氧条件下正构烷烃的藻类修复--综述

Fungal Interactions

Pub Date : 2024-01-30 DOI: 10.1016/j.funint.2024.100001

Kristoffer Palmgren , Magnus Ivarsson

Hydrocarbons released to the environment such as during oil spillages can lead to serious problems for ecosystems, climate, and human health. Many fungi have been shown to degrade various hydrocarbons found in oil and different oil-derived products, making fungi promising for use in bioremediation applications to detoxify hydrocarbon polluted substrates. This review summarises how fungi metabolise and degrade n-alkanes, one of the major components of combustible fuels - including how n-alkanes are taken up by the cell and how different secreted and membrane-bound proteins seem to affect the manner of uptake. Fungi metabolise hydrocarbons by three known oxidation pathways that are catalysed by Cytochrome p450 enzymes. The n-alkanes are not only used as a source of energy but are also incorporated into the cell by lipid synthesis. Many different fungi are already present in oil-contaminated sites and degrade these hydrocarbon pollutants. Yet many fungi seem to have an affinity towards shorter n-alkanes and might not be able to completely degrade all hydrocarbons. Fungi are a great contributor to detoxifying pollutants, but completely removing these hydrocarbon pollutants may require a wide variety of different species and possibly other organisms to effectively remedy contaminated environments.

石油泄漏等情况下释放到环境中的碳氢化合物会给生态系统、气候和人类健康带来严重问题。许多真菌已被证明可以降解石油和不同石油衍生产品中的各种碳氢化合物，这使得真菌有望用于生物修复应用，对碳氢化合物污染的基质进行解毒。本综述总结了真菌如何代谢和降解可燃燃料的主要成分之一--正构烷烃，包括正构烷烃如何被细胞吸收，以及不同的分泌蛋白和膜结合蛋白似乎如何影响吸收方式。真菌通过三种已知的氧化途径代谢碳氢化合物，这些途径由细胞色素 p450 酶催化。正构烷烃不仅被用作能量来源，还通过脂质合成进入细胞。许多不同的真菌已经存在于受石油污染的地方，并能降解这些碳氢化合物污染物。然而，许多真菌似乎对较短的正烷烃具有亲和力，可能无法完全降解所有碳氢化合物。真菌对污染物的解毒作用很大，但要彻底清除这些碳氢化合物污染物，可能需要多种不同的物种，也可能需要其他生物，才能有效修复受污染的环境。

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