SDRP Journal of Cellular and Molecular Physiology最新文献

{"title":"Protective effect of kudzu root vinegar and adenosine against UVB-induced oxidative stress in human keratinocytes","authors":"Ji-Eun Park, Y. M. Kim, Sift Desk Journals Open Access Journals","doi":"10.25177/JCMP.3.2.RA.10673","DOIUrl":"https://doi.org/10.25177/JCMP.3.2.RA.10673","url":null,"abstract":"Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in cells, which induces sunburn cell formation, melanoma, photoaging, and skin cancer. This study examines the anti-photodamage effects of kudzu root vinegar and adenosine in UVB-exposed human keratinocytes (HaCaT cells). UVB significantly decreased HaCaT cell viability, whereas kudzu root vinegar and adenosine did not exhibit cytotoxic effects and increased the viability of HaCaT cells. To investigate the protective effects of kudzu root vinegar and adenosine on UVB-induced oxidative stress in HaCaT cells, ROS, matrix metalloproteinases (MMPs), and mitogen-activated protein kinase (MAPK) were analyzed. UVB-induced treatment reduced the activity of antioxidant enzymes; however, kudzu root vinegar and adenosine increased their activity. These results indicated that kudzu root vinegar and adenosine exert cytoprotective activity against UVB-induced oxidative stress in HaCaT cells. Moreover, they suppressed the UVB-induced downregulation of MMPs and inhibited the phosphorylation of MAPK induced by UVB-irradiation. Therefore, kudzu root vinegar and adenosine offer anti-oxidative effects, via lowering ROS production, suppressing JNK activation, and downregulating expression of MMPs. Our findings suggest that kudzu root vinegar and adenosine have potential application in preventing skin damage owing to UVB exposure. Keywords: reactive oxygen species (ROS), HaCaT cell, UVB, skin damage, anti-aging","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123336798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-20a-3p and microRNA-20a-5p exhibit anti-proliferative activities in a melanoma in vitro model","authors":"M. Stope, Hannes Ahrend, G. Daeschlein, E. Grove, Madeleine Paditz, A. Mustea, M. Burchardt, Sift Desk Journals Open Access Journals","doi":"10.25177/jcmp.3.1.1","DOIUrl":"https://doi.org/10.25177/jcmp.3.1.1","url":null,"abstract":"MicroRNAs control numerous cancer-related signaling pathways and play pivotal role in cancer initiation and progression. Recent studies have indicated variable and cancer-specific expression patterns of microRNA-20a (miR-20a), which have been attended by varying and sometimes contrary tumor biological functions. This is the first study regarding to the characterization of miR-20a's functionality in melanoma cells. miR-20a expression was examined by reverse transcriptase and quantitative polymerase chain reaction in an in vitro melanoma model containing HaCat keratinocytes and B16 melanoma cells. For cell growth analysis, miR-20a vectors were cloned and transfected into B16 cells. Cell growth kinetics were performed utilizing a Cell Counter and Analyzer Model TT (Roche Applied Science). The expression of both the 3p and the 5p strand processed from the miR-20a precursor was suppressed in melanoma cells B16 compared to the expression in non-malignant HaCat keratinocytes. Recombinant restoration of miR -20a levels in malignant B16 cells attenuated cellular growth. Our data suggest that miR-20a bears biological functions in melanoma cells and thus represents an anti-oncogenic factor which is suppressed during","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115108892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemostatic potential of total aqueous extract of Sacoglottis gabonensis (Baille) Urban (Humiriaceae) stem bark in Wistar rats pretreated with warfarin","authors":"Dr. Mama Kone, Nagalo Ousmane, Oussou N’guessan Jean-Baptiste, Yapo Angoué Paul","doi":"10.25177/jcmp.4.1.ra.10817","DOIUrl":"https://doi.org/10.25177/jcmp.4.1.ra.10817","url":null,"abstract":"Background: Sacoglottis gabonensis is a medicinal plant used in traditional treatment of Buruli ulcer and leprosy in Côte d'Ivoire. A study conducted on the healing potential of this herb on induced wounds showed good results. The objective of this study is to evaluate the hemostatic potential of the total aqueous extract of Sacoglottis gabonensis (TAESg) in rats after a pretreatment with warfarin. Methods: Thus, 42 rats were evenly distributed into seven groups of six rats each. The rats in group 1 did not receive treatment whereas those in groups 2, 3, 4, 5, 6 and 7 pretreated with warfarin received by oral route, distilled water, 100 mg/kg body weight (bw) of Vitamin K1 and doses of 3.5; 17.5; 35 and 350 mg/kg bw of TAESg, respectively. Blood samples were collected from the rats’ retro-orbital sinus before the experiment, after induction the blood hypocoagulation with warfarin and after the treatments with vitamin K or the extract in orderto determine hemostatic parameters such as the prothrombin time, the activated partial thromboplastin time and the International Normalized Ratio, the fibrinogen level, the calcium level and the thrombocyte level. Results: The results showed disturbances in the hemostatic parameters of warfarin-induced hypocoagulability rats. The treatments with TAESg significantly normalized these parameters by reducing the prothrombin time, the activated partial thromboplastin time and the INR and by increasing fibrinogen levels. However, the levels of calcium and thrombocytes which were increased after the administration of warfarin did not experience any significant change after the treatments with TAESg or Vitamin K, depending on the group. Conclusion: TAESg has some hemostatic properties similar to those of vitamin K. Key words: hypocoagulation, hemostatic parameters, rats, Sacoglottis gabonensis.","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116558417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NU9056 targets KAT5 to regulate the Proliferation, migration and invasion of esophageal cancer cells via ABCE1 acetylation","authors":"Liang Zong-ying, Huang Jing-tao","doi":"10.25177/jcmp.4.1.ra.10819","DOIUrl":"https://doi.org/10.25177/jcmp.4.1.ra.10819","url":null,"abstract":"Objective: To explore the mechanism by which NU9056 affects the epithelial mesenchymal transformation (EMT), proliferation, migration, and invasion by regulating ABCE1 protein acetylation through KAT5. Methods: TE-1 cells were cultured in vitro, NU9056 concentration by MTT, KAT5 mRN by qRT-PCR, ABCE1 acetylation by immunoprecipitation, KAT5 and EMT-related proteins by Western blot, MTT, migration, and invasion by Transwell. Results: The optimal administration concentration of NU9056 was 1.0 µmol/L via the MTT. In the NU9056 group, KAT5 mRNA and protein expression decreased, and ABCE1 acetylation level decreased (P<0.05); in the NU9056 group, EMT marker protein E-cadherin was downregulated, while N-cadherin and Slug proteins were downregulated (P<0.05). TE-1 cell survival, migration, and invasion were significantly decreased in the NU9056 group (P<0.05). Conclusion: NU9056 may reduce the ABCE1 protein acetylation levels by downregulating KAT5 expression, and subsequently inhibit the EMT, survival, migration, and invasion capacity of esophageal cancer cells. Key words: Acetyltransferase inhibitor NU9056 KAT5 ABCE1, modified by acetylation","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129424452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation and clinical significance of methyltransferases SETDB1 and SPG20 methylation in lung adenocarcinoma","authors":"Zongying Liang, Bao-Hui Zhao, Jingtao Huang","doi":"10.25177/jcmp.4.1.ra.10821","DOIUrl":"https://doi.org/10.25177/jcmp.4.1.ra.10821","url":null,"abstract":"Objective: The incidence of primary lung adenocarcinoma is increasing year by year in worldwide, and it has surpassed squamous cell carcinoma to become the most common pathological type of lung cancer. Advances in medical technology have made the overall survival rate of lung cancer patients improve, but it is still very low. In the study, we investigated the methyltransferase SETDB1 expression and the methylation level of SPG20, and analyzed the correlation and its clinical significance in the pathogenesis of lung adenocarcinoma. Methods: 60 lung adenocarcinoma and normal lung tissues were selected.The expression of SPG20 and SETDB1 mRNA was examined by RT-qPCR; Protein expression of SPG20 and SETDB1 was determined by immunohistochemistry(SP) and immune protein imprinting(Western blot);Methylation level of SPG20 gene was determined by pyrosequencing. Correlation between SETDB1 and SPG20 methylation levels and both and clinical case characteristics was analysed by statistics. Western blot detection of SETDB1 and SPG20 protein expression in lung adenocarcinoma cells and normal bronchial mucosal epithelial cells.The small interfering RNA of SETDB1 was transfected into lung adenocarcinoma A549 cells by liposome-mediated method, the mRNA and protein expression levels of SETDB1 were detected by RT-qPCR and Wesrern blot, and the methylation level of SPG20 gene was detected by pyrosequencing. Results:Relative expression of SPG20 mRNA was significantly lower in lung adenocarcinoma tissue than in normal lung tissue, while relative SETDB1 mRNA expression was higher in cancer tissue than in normal lung tissue. SETDB1 protein expression was significantly higher in lung adenocarcinoma tissue than in normal lung tissue, while SPG20 protein showed lower expression in cancer tissue than in normal lung tissue. The methylation rate of the SPG20 gene was significantly higher in cancerous tissues than in normal lung tissue, the difference was statistically significant. SPG20 methylation in cancer tissues showed a correlation with SETDB1 and showed a positive correlation; SPG20 methylation and SETDB1 are closely related with TNM stage, tissue differentiation, and lymph node metastasis in lung adenocarcinoma. The vitro experiments showed that SETDB1 was highly expressed, while SPG20 was lowIn lung adenocarcinoma cells, SETDB1 was low and SPG20 was high in normal bronchial epithelial cells. RNA interference with SETDB1 could significantly reduce the mRNA and protein expression of SETDB1 in A549 cells, the methylation rate of SPG20 gene was significantly decreased, and the protein expression was increased. Conclusion:There is a significant correlation between methyltransferases SETDB1 and SPG20 methylation in lung adenocarcinoma, and high SETDB1 expression may be the upstream molecule of SPG20 methylation, working together in promoting the occurrence and development of lung adenocarcinoma.","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123357711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ATRA on proliferation, migration and cell cycle of gastric cancer MGC-803 cells-siftdesk","authors":"Liu Sha-sha, Yan SHI Ya-nan","doi":"10.25177/jcmp.4.1.ra.10816","DOIUrl":"https://doi.org/10.25177/jcmp.4.1.ra.10816","url":null,"abstract":"Objective：To study the effects of all-trans-retinoic acid (ATRA) on proliferation, migration and cycle of human gastric cancer MGC-803 cells. Methods：The proliferation activity of gastric cancer MGC-803 cells treated with different concentrations of ATRA was detected by CCK-8 assay. The morphological changes of MGC-803 cells in each group were observed under inverted microscope. The migration ability of cells in each group was detected by scratch test. Flow cytometry was used to detect the change of MGC-803 cell cycle. Results：CCK-8 results showed that ATRA could significantly inhibit the proliferation of MGC-803 cells, and the inhibition rate of cell proliferation increased gradually with the increasing of ATRA concentrations and action time, in a dose-time dependent manner(P<0.05). Under inverted microscope, the morphology of MGC-803 cells changed and the number of viable cells decreased significantly after ATRA treatment. The results of scratch assay showed that ATRA could significantly inhibit the migration of gastric cancer MGC-803 cells, and the repair rate of scratch area decreased significantly with the increasing of ATRA concentration(P<0.05).Flow cytometry showed that the proportion of G0/G1 phase cells increased significantly after ATRA treatment, and the proportion of S phase cells decreased (P<0.05).And there was no significant change in G2/M phase cell proportion (P>0.05).","PeriodicalId":102720,"journal":{"name":"SDRP Journal of Cellular and Molecular Physiology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125483085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}