{"title":"Properties","authors":"R. Puffr, Miroslav Raab, B. Doležel","doi":"10.1201/9780367813062-6","DOIUrl":"https://doi.org/10.1201/9780367813062-6","url":null,"abstract":"","PeriodicalId":120222,"journal":{"name":"Lactam-Based Polyamides","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126684102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
...............................................................................................................................3 Acknowledgements..............................................................................................................4 List of Tables .......................................................................................................................7 List of Figures ......................................................................................................................8 Chapter 1 Gravitropism....................................................................................................9 The Gravitropic Pathway .......................................................................................10 Perception ..................................................................................................10 Auxin Redistribution..................................................................................13 Response ....................................................................................................16 Signal Transduction ...................................................................................17 Research Focus ......................................................................................................22 Bibliography ..........................................................................................................25 Chapter 2 Characterization of the GPS2 Gene ..............................................................31 Materials and Methods.............................................................................................32 Plant Material, Growth Conditions, and DNA Extraction.........................32 Thermal Asymmetric Interlaced Polymerase Chain Reaction ..................32 Cloning of the PCR Product ......................................................................36 Constructs ..................................................................................................38 Agrobacterium Transformation .................................................................43 Floral Dip and Arabidopsis Transformation ..............................................43