Pub Date : 2021-06-02DOI: 10.2174/9781681088082121010003
Neeraj Mittal, S. Bhadada, O. Katare, Varun Garg
{"title":"List of Contributors","authors":"Neeraj Mittal, S. Bhadada, O. Katare, Varun Garg","doi":"10.2174/9781681088082121010003","DOIUrl":"https://doi.org/10.2174/9781681088082121010003","url":null,"abstract":"","PeriodicalId":121110,"journal":{"name":"COVID-19: Diagnosis and Management-Part I","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133856834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-02DOI: 10.2174/9781681088082121010009
R. Deshpande, Aishwarya P. Joshi, N. Tandel, R. Tyagi
Summary IKAROS, a zinc finger transcription factor protein encoded by the IKZF1 gene is crucial for hematopoiesis in humans. Loss of function mutations in IKZF1 have been implicated in adult and pediatric B cell Acute Lymphoblastic Leukemia (B-ALL). Our aim is to evaluate the incidence of IKAROS protein expression and IKZF1 mRNA transcripts in B-ALL patients. IKAROS protein expression was evaluated in total 34 patients diagnosed as de-novo B-ALL using flow cytometry and IKZF1 mRNA Transcripts were detected using reverse transcriptase - polymerase chain reaction (RT-PCR) method in 13 patients. The expression levels of the IKAROS protein and IKZF1 isoforms were analysed and correlated with clinical and hematological parameters. B-ALL patients with presence of only IK-6 isoform were associated with high WBC count, blast count (>85%) as well as progenitor cell markers CD34 and Tdt positivity. Also there was a significant correlation of low hemoglobin and CD19 positivity with IKAROS protein over expression. In one of the patient we found only 09% of IKAROS protein, having two dominant negative isoforms IK-4 and IK-6 with absence of normal isoforms, which might suggest disease aggressiveness. However, IKAROS protein evaluation by flow cytometry method should be correlated with IKAROS isoforms in more number of B-ALL patients so that the clinical relevance of IKAROS protein could be established. Correlation with conventional prognostic markers suggests that the patients showing the presence of IK-6 isoform can be considered as high risk patients.
{"title":"Diagnosis","authors":"R. Deshpande, Aishwarya P. Joshi, N. Tandel, R. Tyagi","doi":"10.2174/9781681088082121010009","DOIUrl":"https://doi.org/10.2174/9781681088082121010009","url":null,"abstract":"Summary IKAROS, a zinc finger transcription factor protein encoded by the IKZF1 gene is crucial for hematopoiesis in humans. Loss of function mutations in IKZF1 have been implicated in adult and pediatric B cell Acute Lymphoblastic Leukemia (B-ALL). Our aim is to evaluate the incidence of IKAROS protein expression and IKZF1 mRNA transcripts in B-ALL patients. IKAROS protein expression was evaluated in total 34 patients diagnosed as de-novo B-ALL using flow cytometry and IKZF1 mRNA Transcripts were detected using reverse transcriptase - polymerase chain reaction (RT-PCR) method in 13 patients. The expression levels of the IKAROS protein and IKZF1 isoforms were analysed and correlated with clinical and hematological parameters. B-ALL patients with presence of only IK-6 isoform were associated with high WBC count, blast count (>85%) as well as progenitor cell markers CD34 and Tdt positivity. Also there was a significant correlation of low hemoglobin and CD19 positivity with IKAROS protein over expression. In one of the patient we found only 09% of IKAROS protein, having two dominant negative isoforms IK-4 and IK-6 with absence of normal isoforms, which might suggest disease aggressiveness. However, IKAROS protein evaluation by flow cytometry method should be correlated with IKAROS isoforms in more number of B-ALL patients so that the clinical relevance of IKAROS protein could be established. Correlation with conventional prognostic markers suggests that the patients showing the presence of IK-6 isoform can be considered as high risk patients.","PeriodicalId":121110,"journal":{"name":"COVID-19: Diagnosis and Management-Part I","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114286721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：