Pub Date : 1999-08-01DOI: 10.1002/(SICI)1521-4168(19990801)22:8<454::AID-JHRC454>3.0.CO;2-#
L. Rohrschneider
{"title":"Prototypical Test Substances for a Reversed Phase in a Retention Parameter Model","authors":"L. Rohrschneider","doi":"10.1002/(SICI)1521-4168(19990801)22:8<454::AID-JHRC454>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19990801)22:8<454::AID-JHRC454>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"21 1","pages":"454-458"},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91545856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-06-01DOI: 10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#
J. Han, Y. Iwasaki, T. Yamane
A high resolution approach to silver ion HPLC was studied for the separation of positional isomers of triacylglycerols (TAGs) containing long chain polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and docosapentaenoic acid (DPA) in enzymatically synthesized structured TAGs. Isopropanol was used as a novel modifier in a hexane-acetonitrile based mobile phase for silver ion HPLC. Peak identification was based on HPLC-mass spectroscopy and selectivities of lipases. Positional isomers of TAGs containing one molecule of EPA, DHA, or DPA with saturated fatty acids (FAs) such as caprylic acid and palmitic acid were separated within 13 min using a gradient of hexane-isopropanol-acetonitrile as mobile phase. TAGs containing two or more EPA, DHA, or DPA were also separated from each other within 25 min, but their positional isomers were unresolved. The retention characteristics of the TAG were found to be related to the number of carbon atoms in the FAs present in addition to the number of double bonds and their isomeric configuration. One isomer with an unsaturated FA in the sn-2 position eluted faster than the other with the unsaturated FA in the sn-1 or 3 position. Species with longer chain FAs attached to TAGs with the same degree of unsaturation eluted faster than those that have shorter chain FAs. For example, docosapentaenoylhexadecanoyloctanoin (DPA/C16/C8) was eluted faster than dioctanoyldocosapentaenoin (DPA/C8/C8).
{"title":"Use of isopropanol as a modifier in a hexane-acetonitrile based mobile phase for the silver ion HPLC separation of positional isomers of triacylglycerols containing long chain polyunsaturated fatty acids","authors":"J. Han, Y. Iwasaki, T. Yamane","doi":"10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#","url":null,"abstract":"A high resolution approach to silver ion HPLC was studied for the separation of positional isomers of triacylglycerols (TAGs) containing long chain polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and docosapentaenoic acid (DPA) in enzymatically synthesized structured TAGs. Isopropanol was used as a novel modifier in a hexane-acetonitrile based mobile phase for silver ion HPLC. Peak identification was based on HPLC-mass spectroscopy and selectivities of lipases. Positional isomers of TAGs containing one molecule of EPA, DHA, or DPA with saturated fatty acids (FAs) such as caprylic acid and palmitic acid were separated within 13 min using a gradient of hexane-isopropanol-acetonitrile as mobile phase. TAGs containing two or more EPA, DHA, or DPA were also separated from each other within 25 min, but their positional isomers were unresolved. The retention characteristics of the TAG were found to be related to the number of carbon atoms in the FAs present in addition to the number of double bonds and their isomeric configuration. One isomer with an unsaturated FA in the sn-2 position eluted faster than the other with the unsaturated FA in the sn-1 or 3 position. Species with longer chain FAs attached to TAGs with the same degree of unsaturation eluted faster than those that have shorter chain FAs. For example, docosapentaenoylhexadecanoyloctanoin (DPA/C16/C8) was eluted faster than dioctanoyldocosapentaenoin (DPA/C8/C8).","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"109 4 1","pages":"357-361"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83625563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-06-01DOI: 10.1002/(SICI)1521-4168(19990601)22:6<339::AID-JHRC339>3.0.CO;2-#
S. Buskov, H. Sørensen, S. Sørensen
Complex mixtures of chlorophyll degradation products may arise during processing and storage of vegetable oil and green plant materials like broccoli and spinach. Determination of these compounds is important in the area of food chemistry. Therefore a method using packed column supercritical fluid chromatography (SFC) has been developed. The method comprises chromatography using a simple gradient of methanol in carbon dioxide at constant column back pressure of 30 MPa and a column temperature of 40°C. Effects of pressure and mobile phase composition showed the importance of applying a modifier gradient for optimal separation of the chlorophyll products. The method permits separation of 15 chlorophyll derivatives including chlorophyll a and b, pheophytins, and pyropheophytins on a C18 column in about 20 minutes. Identifications of the individual peaks were based on reference compounds, the retention order of the compounds, and their absorption spectra.
{"title":"Separation of Chlorophylls and Their Degradation Products using Packed Column Supercritical Fluid Chromatography (SFC)","authors":"S. Buskov, H. Sørensen, S. Sørensen","doi":"10.1002/(SICI)1521-4168(19990601)22:6<339::AID-JHRC339>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19990601)22:6<339::AID-JHRC339>3.0.CO;2-#","url":null,"abstract":"Complex mixtures of chlorophyll degradation products may arise during processing and storage of vegetable oil and green plant materials like broccoli and spinach. Determination of these compounds is important in the area of food chemistry. Therefore a method using packed column supercritical fluid chromatography (SFC) has been developed. The method comprises chromatography using a simple gradient of methanol in carbon dioxide at constant column back pressure of 30 MPa and a column temperature of 40°C. Effects of pressure and mobile phase composition showed the importance of applying a modifier gradient for optimal separation of the chlorophyll products. The method permits separation of 15 chlorophyll derivatives including chlorophyll a and b, pheophytins, and pyropheophytins on a C18 column in about 20 minutes. Identifications of the individual peaks were based on reference compounds, the retention order of the compounds, and their absorption spectra.","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"115 1","pages":"339-342"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79511728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-11-01DOI: 10.1002/(SICI)1521-4168(19981101)21:11<620::AID-JHRC620>3.0.CO;2-#
R. M. Kinghorn, P. Marriott
{"title":"Comprehensive two-dimensional gas chromatography using a modulating cryogenic trap","authors":"R. M. Kinghorn, P. Marriott","doi":"10.1002/(SICI)1521-4168(19981101)21:11<620::AID-JHRC620>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19981101)21:11<620::AID-JHRC620>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"11 1","pages":"620-622"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82226433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-09-01DOI: 10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#
D. Seibert, C. Poole
A general model is presented for the prediction of experimental conditions for the isolation of organic compounds from aqueous solution by solid-phase extraction. The solvation parameter model is used to predict the variation of retention as a function of solvent composition from characteristic solute descriptors and combined with a frontal analysis model to adjust the predicted breakthrough volumes to account for the influence of the small number of theoretical plates characteristic of sorbent cartridges. System maps are used to determine breakthrough volumes for sample processing, the composition and volume of wash solvents for matrix simplification, and the composition and volume of eluting solvents. System maps are provided for an octadecylsiloxane-bonded silica sorbent with methanol and acetonitrile as cosolvents with water. These maps are used to calculate the experimental conditions for the isolation of estrogens from urine and the model validated by experimental verification of the model predictions. The proposed approach provides a simple theoretical framework for computer-aided method development in solid-phase extraction.
{"title":"A General Model for the Optimization of Sample Processing Conditions by Solid‐Phase Extraction Applied to the Isolation of Estrogens from Urine","authors":"D. Seibert, C. Poole","doi":"10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#","url":null,"abstract":"A general model is presented for the prediction of experimental conditions for the isolation of organic compounds from aqueous solution by solid-phase extraction. The solvation parameter model is used to predict the variation of retention as a function of solvent composition from characteristic solute descriptors and combined with a frontal analysis model to adjust the predicted breakthrough volumes to account for the influence of the small number of theoretical plates characteristic of sorbent cartridges. System maps are used to determine breakthrough volumes for sample processing, the composition and volume of wash solvents for matrix simplification, and the composition and volume of eluting solvents. System maps are provided for an octadecylsiloxane-bonded silica sorbent with methanol and acetonitrile as cosolvents with water. These maps are used to calculate the experimental conditions for the isolation of estrogens from urine and the model validated by experimental verification of the model predictions. The proposed approach provides a simple theoretical framework for computer-aided method development in solid-phase extraction.","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"68 1","pages":"481-490"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74113791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-08-01DOI: 10.1002/(SICI)1521-4168(19980801)21:8<464::AID-JHRC464>3.0.CO;2-#
M. Hyun, Young Duk Kim, S. Han, Jung Bae Lee
{"title":"A Chiral Recognition Mechanism Proposed for Resolving π‐Acidic Racemates on π‐Acidic Chiral Stationary Phases Derived from (S)‐Leucine","authors":"M. Hyun, Young Duk Kim, S. Han, Jung Bae Lee","doi":"10.1002/(SICI)1521-4168(19980801)21:8<464::AID-JHRC464>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19980801)21:8<464::AID-JHRC464>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"25 1","pages":"464-470"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90691005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-05-01DOI: 10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#
T. Kaneta, Hyuk Jeong, E. Yeung
{"title":"Miniaturized injection method using silver-coated capillaries in DNA sequencing by multiplexed capillary electrophoresis","authors":"T. Kaneta, Hyuk Jeong, E. Yeung","doi":"10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"1 1","pages":"287-290"},"PeriodicalIF":0.0,"publicationDate":"1998-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79306406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1982-04-01DOI: 10.1007/978-94-009-7642-9_14
F. Chialva, G. Gabri, P. Liddle, Franco Ulian
{"title":"Qualitative Evaluation of Aromatic Herbs by Direct Head Space (GC)2 Analysis. Applications of the Method and Comparison with the Traditional Analysis of Essential Oils","authors":"F. Chialva, G. Gabri, P. Liddle, Franco Ulian","doi":"10.1007/978-94-009-7642-9_14","DOIUrl":"https://doi.org/10.1007/978-94-009-7642-9_14","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"43 1","pages":"183-195"},"PeriodicalIF":0.0,"publicationDate":"1982-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77333738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ion pair chromatography on reversed‐phase layers","authors":"Dieter-Günter Volkmann","doi":"10.1007/BFB0024398","DOIUrl":"https://doi.org/10.1007/BFB0024398","url":null,"abstract":"","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"1 1","pages":"51-69"},"PeriodicalIF":0.0,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76824477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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