Pub Date : 2023-06-01DOI: 10.1007/s11726-023-1379-z
Danyang Zhao, Qiang Wang
{"title":"推拿联合补中益气汤治疗脱肛临床观察","authors":"Danyang Zhao, Qiang Wang","doi":"10.1007/s11726-023-1379-z","DOIUrl":"https://doi.org/10.1007/s11726-023-1379-z","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136161449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1007/s11726-023-1373-5
Tongjun Ma, Wen Dong, Hua-chun Miao, Feng Wu
{"title":"Electroacupuncture stimulation attenuates corpus striatum white matter injury in rats with cerebral ischemia by inhibition of Nogo-A/NgR pathway","authors":"Tongjun Ma, Wen Dong, Hua-chun Miao, Feng Wu","doi":"10.1007/s11726-023-1373-5","DOIUrl":"https://doi.org/10.1007/s11726-023-1373-5","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46359963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1007/s11726-023-1381-5
Pengfei Li, Min Xiao, Xuejiao Ma, Xingke Yan, Chong-bing Ma
{"title":"Acupuncture intervening depressive disorder: research progress in its neurobiological mechanism","authors":"Pengfei Li, Min Xiao, Xuejiao Ma, Xingke Yan, Chong-bing Ma","doi":"10.1007/s11726-023-1381-5","DOIUrl":"https://doi.org/10.1007/s11726-023-1381-5","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44584885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Objective To investigate the analgesic mechanism of Tuina (Chinese therapeutic massage) by observing the effect of the N-methyl-D-aspartate receptor subunit 2B (NR2B)/postsynaptic density-95 ( P SD-95) pathway on the dendritic structure of spinal cord dorsal horn in rats with lumbar disc herniation. Methods Fifty Sprague-Dawley rats were randomly divided into a blank group, a model group, a Tuina group, a blocker agent group, and a blocker agent + Tuina group. The sciatic nerve chronic constriction injury (CCI) model was prepared by the sciatic nerve ligation method. From the 4th day after modeling, rats in the Tuina group and the blocker agent + Tuina group were subject to daily Tuina intervention, and those in the blocker agent group and the blocker agent + Tuina group were daily intrathecally injected with NR2B blocker agent (MK-801). The spontaneous pain score was used to observe the pain behavior of all rats. The expression levels of NR2B and downstream PSD-95 were measured by immunohistochemistry, and the dendritic structure changes were observed by Golgi staining for rat spinal cord dorsal horn after 14 d of continuous intervention. Results Compared with the blank group, the degree of rat spontaneous pain after CCI was elevated in both the model and the Tuina groups ( P <0.01) and was reduced in the Tuina group after the Tuina intervention compared with the model group ( P <0.05). Compared with the model group, the rat spontaneous pain level after blocking NR2B was reduced in both the blocker agent group and the blocker agent + Tuina group ( P <0.05). The NR2B and PSD-95 protein levels were significantly higher in the model group compared with the blank group ( P <0.01); the total number of dendritic branches was increased ( P <0.01), and the total dendritic length became longer ( P <0.01) in the spinal cord dorsal horn. The rat NR2B and PSD-95 protein levels were significantly decreased in the Tuina group compared with the model group ( P <0.01); the total dendritic branch number was reduced ( P <0.01) and the total length was shortened ( P <0.01) in the spinal cord dorsal horn. After blocking NR2B, the expression levels of NR2B and downstream PSD-95 protein were significantly lower in both the blocker agent group and the blocker agent + Tuina group compared to the model group ( P <0.01). The total branch number was significantly reduced ( P <0.01), and the total length was significantly shortened ( P <0.01) of the dendrites in the spinal cord dorsal horn. Conclusion Tuina may exert an analgesic effect by remodeling the dendritic structure in the spinal cord dorsal horn in rats with lumbar disc herniation, and its mechanism may be related to the inhibition of NR2B/PSD-95 signaling pathway.
{"title":"基于NR2B/PSD-95通路探讨推拿对腰椎间盘突出症大鼠脊髓背角树突结构的影响","authors":"Huanzhen Zhang, Bingqian Wang, Shuijin Chen, Lechun Chen, Jingjing Jiang, Yu Jiang, Jincheng Chen, Hongye Huang, Jiayu Fang, Weiquan Zeng, Zhigang Lin","doi":"10.1007/s11726-023-1368-2","DOIUrl":"https://doi.org/10.1007/s11726-023-1368-2","url":null,"abstract":"Abstract Objective To investigate the analgesic mechanism of Tuina (Chinese therapeutic massage) by observing the effect of the N-methyl-D-aspartate receptor subunit 2B (NR2B)/postsynaptic density-95 ( P SD-95) pathway on the dendritic structure of spinal cord dorsal horn in rats with lumbar disc herniation. Methods Fifty Sprague-Dawley rats were randomly divided into a blank group, a model group, a Tuina group, a blocker agent group, and a blocker agent + Tuina group. The sciatic nerve chronic constriction injury (CCI) model was prepared by the sciatic nerve ligation method. From the 4th day after modeling, rats in the Tuina group and the blocker agent + Tuina group were subject to daily Tuina intervention, and those in the blocker agent group and the blocker agent + Tuina group were daily intrathecally injected with NR2B blocker agent (MK-801). The spontaneous pain score was used to observe the pain behavior of all rats. The expression levels of NR2B and downstream PSD-95 were measured by immunohistochemistry, and the dendritic structure changes were observed by Golgi staining for rat spinal cord dorsal horn after 14 d of continuous intervention. Results Compared with the blank group, the degree of rat spontaneous pain after CCI was elevated in both the model and the Tuina groups ( P <0.01) and was reduced in the Tuina group after the Tuina intervention compared with the model group ( P <0.05). Compared with the model group, the rat spontaneous pain level after blocking NR2B was reduced in both the blocker agent group and the blocker agent + Tuina group ( P <0.05). The NR2B and PSD-95 protein levels were significantly higher in the model group compared with the blank group ( P <0.01); the total number of dendritic branches was increased ( P <0.01), and the total dendritic length became longer ( P <0.01) in the spinal cord dorsal horn. The rat NR2B and PSD-95 protein levels were significantly decreased in the Tuina group compared with the model group ( P <0.01); the total dendritic branch number was reduced ( P <0.01) and the total length was shortened ( P <0.01) in the spinal cord dorsal horn. After blocking NR2B, the expression levels of NR2B and downstream PSD-95 protein were significantly lower in both the blocker agent group and the blocker agent + Tuina group compared to the model group ( P <0.01). The total branch number was significantly reduced ( P <0.01), and the total length was significantly shortened ( P <0.01) of the dendrites in the spinal cord dorsal horn. Conclusion Tuina may exert an analgesic effect by remodeling the dendritic structure in the spinal cord dorsal horn in rats with lumbar disc herniation, and its mechanism may be related to the inhibition of NR2B/PSD-95 signaling pathway.","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135518240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1007/s11726-023-1371-7
Xu-Hong Zhao, L. Dong, Ming Zhang, Surong He, Qixiong Chen, X. Cao
{"title":"Therapeutic efficacy evaluation of Tuina at points on abdomen and back meridians for infantile colic","authors":"Xu-Hong Zhao, L. Dong, Ming Zhang, Surong He, Qixiong Chen, X. Cao","doi":"10.1007/s11726-023-1371-7","DOIUrl":"https://doi.org/10.1007/s11726-023-1371-7","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44074311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1007/s11726-023-1370-8
Y. Li, Tao Yang, Lin-Yu Huang
{"title":"巨刺联合麦特兰德踝关节松动术治疗陈旧性踝关节扭伤的临床观察","authors":"Y. Li, Tao Yang, Lin-Yu Huang","doi":"10.1007/s11726-023-1370-8","DOIUrl":"https://doi.org/10.1007/s11726-023-1370-8","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46916344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1007/s11726-023-1367-3
Li-Bin Wu, Fan Zhang, Qing Yu, Lei Liu, Xiao-Tong Wei, Rong-Lin Cai, Zijian Wu, Ling Hu
{"title":"Study on reverse transsynaptic virus tracing targeting Shenmen (HT7) and heart in mice","authors":"Li-Bin Wu, Fan Zhang, Qing Yu, Lei Liu, Xiao-Tong Wei, Rong-Lin Cai, Zijian Wu, Ling Hu","doi":"10.1007/s11726-023-1367-3","DOIUrl":"https://doi.org/10.1007/s11726-023-1367-3","url":null,"abstract":"","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48245673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.1007/s11726-023-1366-4
Jiayi Chen, Xiaoji Zhu, Yang Peng, Huirong Liu, Huangan Wu, Cili Zhou
Abstract Objective To observe the effect of moxibustion on behaviors and related products of tryptophan (Trp) metabolism in the colon of mice with irritable bowel syndrome (IBS), and to explore the mechanism of moxibustion in the IBS treatment. Methods Twenty-four mice were randomly divided into a normal group, a model group, a moxibustion group, and a probiotic group, with 6 mice in each group. The visceral pain model of IBS was established by enema with 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution. Mice in the moxibustion group were treated with mild moxibustion at bilateral Zusanli (ST36), and those in the probiotic group were treated with probiotics such as Bifidobacterium by gavage. Abdominal withdrawal reflex (AWR) test, elevated plus-maze (EPM) test, and forced swimming test (FST) were performed after treatment. The expression levels of 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (TPH1) in the colon were detected by immunofluorescence, and the expression levels of Trp, kynurenine (Kyn), and indole-2,3-oxygenase (IDO) in the colon were detected by enzyme-linked immunosorbent assay. Results Compared with the normal group, the AWR scores were increased significantly in the model group under different pressure values ( P <0.01), the open-arm staying time and open-arm entries in the EPM test were decreased significantly ( P <0.01, P <0.05), the motionless time in the FST was increased significantly ( P <0.01), and the expression levels of colonic Trp, TPH1, IDO, 5-HT, and Kyn were increased significantly ( P <0.01) in the models. Compared with the model group, the AWR scores were differently decreased ( P <0.05 or P <0.01), the open-arm entries in the EPM test were increased ( P <0.05), the motionless times in the FST were decreased ( P <0.05), and the colonic expression levels of Trp, TPH1, IDO, and 5-HT were decreased ( P <0.01 or P <0.05) in the moxibustion and probiotic groups; the open-arm staying time was significantly increased in the moxibustion group ( P <0.01), and the colonic expression level of Kyn was significantly decreased in the probiotic group ( P <0.01). Conclusion Moxibustion at Zusanli (ST36) improves visceral pain and pain mood and down-regulates the expression levels of colonic TPH1, IDO, Trp, 5-HT, and Kyn in IBS mice.
{"title":"艾灸对肠易激综合征小鼠行为学及色氨酸代谢相关产物的影响","authors":"Jiayi Chen, Xiaoji Zhu, Yang Peng, Huirong Liu, Huangan Wu, Cili Zhou","doi":"10.1007/s11726-023-1366-4","DOIUrl":"https://doi.org/10.1007/s11726-023-1366-4","url":null,"abstract":"Abstract Objective To observe the effect of moxibustion on behaviors and related products of tryptophan (Trp) metabolism in the colon of mice with irritable bowel syndrome (IBS), and to explore the mechanism of moxibustion in the IBS treatment. Methods Twenty-four mice were randomly divided into a normal group, a model group, a moxibustion group, and a probiotic group, with 6 mice in each group. The visceral pain model of IBS was established by enema with 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution. Mice in the moxibustion group were treated with mild moxibustion at bilateral Zusanli (ST36), and those in the probiotic group were treated with probiotics such as Bifidobacterium by gavage. Abdominal withdrawal reflex (AWR) test, elevated plus-maze (EPM) test, and forced swimming test (FST) were performed after treatment. The expression levels of 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (TPH1) in the colon were detected by immunofluorescence, and the expression levels of Trp, kynurenine (Kyn), and indole-2,3-oxygenase (IDO) in the colon were detected by enzyme-linked immunosorbent assay. Results Compared with the normal group, the AWR scores were increased significantly in the model group under different pressure values ( P <0.01), the open-arm staying time and open-arm entries in the EPM test were decreased significantly ( P <0.01, P <0.05), the motionless time in the FST was increased significantly ( P <0.01), and the expression levels of colonic Trp, TPH1, IDO, 5-HT, and Kyn were increased significantly ( P <0.01) in the models. Compared with the model group, the AWR scores were differently decreased ( P <0.05 or P <0.01), the open-arm entries in the EPM test were increased ( P <0.05), the motionless times in the FST were decreased ( P <0.05), and the colonic expression levels of Trp, TPH1, IDO, and 5-HT were decreased ( P <0.01 or P <0.05) in the moxibustion and probiotic groups; the open-arm staying time was significantly increased in the moxibustion group ( P <0.01), and the colonic expression level of Kyn was significantly decreased in the probiotic group ( P <0.01). Conclusion Moxibustion at Zusanli (ST36) improves visceral pain and pain mood and down-regulates the expression levels of colonic TPH1, IDO, Trp, 5-HT, and Kyn in IBS mice.","PeriodicalId":14826,"journal":{"name":"Journal of Acupuncture and Tuina Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135518238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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