Pub Date : 2021-05-31DOI: 10.24966/CIIT-8844/1000063
Xiiang Yang
Comparison of the SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Assay and Direct Binding ELISA (S-IgG) with the Cytopathic Effect Assay (CPE) in Analyzing the Neutralization of Vaccination Abstract The safety and effectiveness of the COVID19 vaccine are the key in vaccine development. Due to the difficulty and dangerous of virus neutralization experiment, many replacement assays were de- veloped for testing neutralization antibody. In this report, two kits that based on the competition assay (cPass sVAT) and based on direct antigen binding assay(S-IgG) were compared. The positive rate of cPass sVAT kit and S-IgG assay was 72.9% and 84.2% respectively in 59 sample of vaccinators. Both kit’s specificities reached 100% in 46 health control samples. Quantity analysis of the S-IgG results found that 94.9% (56/59) of vaccinators have produced neutralizing antibodies. The total coincidence rate between cPass sVAT and CPE was 86%, similar to the coincidence rate between S-IgG and CPE, which was 84.2%. S-IgG is relatively more sensitive and easier in quantitating the neutralization antibodies than the other two experi- ments.
{"title":"Comparison of the SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Assay and Direct Binding ELISA (S-IgG) with the Cytopathic Effect Assay (CPE) in Analyzing the Neutralization Antibody of Vaccination People","authors":"Xiiang Yang","doi":"10.24966/CIIT-8844/1000063","DOIUrl":"https://doi.org/10.24966/CIIT-8844/1000063","url":null,"abstract":"Comparison of the SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Assay and Direct Binding ELISA (S-IgG) with the Cytopathic Effect Assay (CPE) in Analyzing the Neutralization of Vaccination Abstract The safety and effectiveness of the COVID19 vaccine are the key in vaccine development. Due to the difficulty and dangerous of virus neutralization experiment, many replacement assays were de- veloped for testing neutralization antibody. In this report, two kits that based on the competition assay (cPass sVAT) and based on direct antigen binding assay(S-IgG) were compared. The positive rate of cPass sVAT kit and S-IgG assay was 72.9% and 84.2% respectively in 59 sample of vaccinators. Both kit’s specificities reached 100% in 46 health control samples. Quantity analysis of the S-IgG results found that 94.9% (56/59) of vaccinators have produced neutralizing antibodies. The total coincidence rate between cPass sVAT and CPE was 86%, similar to the coincidence rate between S-IgG and CPE, which was 84.2%. S-IgG is relatively more sensitive and easier in quantitating the neutralization antibodies than the other two experi- ments.","PeriodicalId":148952,"journal":{"name":"Clinical Immunology & Immunotherapy","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128522256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-31DOI: 10.24966/CIIT-8844/1000056
M. Corsi
{"title":"Physical and Psychological Care of Health Care Workers During COVID-19 Outbreak: A Difficult Challenge","authors":"M. Corsi","doi":"10.24966/CIIT-8844/1000056","DOIUrl":"https://doi.org/10.24966/CIIT-8844/1000056","url":null,"abstract":"","PeriodicalId":148952,"journal":{"name":"Clinical Immunology & Immunotherapy","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121924060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：