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Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama 探索、隔离和识别感染害虫的昆虫病原体真菌
Pub Date : 2020-07-23 DOI: 10.19184/jptt.v1i2.18554
Arsi Arsi, Yuliana Pujiastuti, Suparman Surya Hadi Kusuma, Bambang Gunawan
ABSTRACT Entomopathogenic fungi are classified as a fungus that infects insect pests. Hyphae color that comes out of the insect's body depends on the type of entomopathogenic fungus that attacks it. The purpose of this research is to explore, isolate and identify entomopathogenic fungi that attack insect pests. Samples of dead insects were taken from vegetable crops in the highlands of Pagaralam City and the lowlands of Ogan Komering Ilir Regency. In addition, exploration is also carried out using insect bait methods. This method is carried out on soils taken from the highlands of the fencealam area and Pekanbaru area. Based on the results of entomopathogenic expolation in vegetable plants that infect insects in the field there are 2 types of fungi that attack the insect. Two types of entomapatogenic fungi were found to have characteristics, namely the first hyphal color is rather green which covers the entire body of the insect and the second is white hyphae. The fungus that attacks the insect, then isolated to the GYA media found two entomopathogenic fungi namely, Metarhizium sp. and Beauveria bassiana. Meanwhile, through insect bait using soil T. molitor larvae, one species of entomopathogenic fungus was found, namely Metarhizium sp. The most infected T. molitor larvae in the soil of origin of Pekanbaru in the 2nd and 4th weeks, namely, 21.90 and 29.33 tails.   . Keywords: Entomopathogenic Fungus, Insect Pest and Tenibrio molitor  
昆虫病原真菌是一种感染害虫的真菌。昆虫体内产生的菌丝颜色取决于攻击它的昆虫病原真菌的类型。本研究的目的是探索、分离和鉴定攻击害虫的昆虫病原真菌。死亡昆虫的样本是从帕加拉拉姆市高地和奥甘·科莫林·伊里尔摄政区低地的蔬菜作物中提取的。此外,还采用虫饵法进行了探索。这种方法是在围栏地区和北干巴鲁地区高地的土壤上进行的。根据田间侵染昆虫的蔬菜植物昆虫病原学调查结果,侵染昆虫的真菌有2种。发现两种昆虫致病真菌具有以下特点:第一种菌丝颜色偏绿,覆盖整个虫体;第二种菌丝颜色为白色。将攻击昆虫的真菌分离到GYA培养基上,发现两种昆虫病原真菌,分别是绿僵菌和球孢白僵菌。与此同时,通过以土中褐飞虱幼虫为饵料,发现了一种昆虫病原真菌,即绿僵菌(Metarhizium sp.)。北干巴山原产土中褐飞虱幼虫在第2周和第4周感染最多,分别为21.90尾和29.33尾。关键词:昆虫病原真菌;害虫
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引用次数: 3
Efektivitas dan selektivitas beberapa bahan aktif herbisida untuk mengendalikan gulma pada dua varietas tanaman kacang panjang (Vigna sesquipedalis L.) 几种活性除草剂的有效和选择性,以控制两种长豆科植物的杂草(Vigna sesquatmarlis L)。
Pub Date : 2020-07-15 DOI: 10.19184/jptt.v1i2.17917
W. Purnomo, Saifuddin Hasjim
Field experiments on the effectiveness and selectivity of herbicides use several active ingredients there were Sulfentrazone, Ethyl Pirazosulfuron, and Oxifluorfen with 2 varieties there were Peleton and Kanton tavi. The purpose of the study was to discover the most effective and selective herbicide active ingredients. The study was conducted from February to May 2019. This study used a randomized block design with 7 treatments, namely A (Kontrol), B (Oksifluorfen, peleton), C (Etil pirazosulfuron, peleton), D (Sulfentrazon, peleton), E (Oksifluorfen, kanton tavi), F (Etil pirazosulfuron, kanton tavi), G (Sulfentrazon, kanton tavi). Each treatment was repeated 4 times. The data were analyzed used analysis of variance (ANOVA), further testing used the DMRT test with a 5 % level. The results showed that the application of herbicides made from sulfentrazone, ethyl pirazosulfuron, oxifluorfen in peleton varieties and kanton tavi can suppress weed growth in the research area. The best weed control results were shown by herbicide treatment with sulfentrazone as active ingredient in the kanton tavi variant. The best long bean production results were shown by herbicide treatment with sulfentrazone in peleton varieties. The application of herbicides with active oxifluorfen made no symptoms of phytotoxicity, but decreased the yield of kanton tavi varieties and peleton varieties.
田间药效和选择性试验采用几种有效成分:磺胺曲酮、吡唑磺隆乙基和氟氧芬,品种有培立顿和康顿塔维。研究的目的是发现最有效和选择性的除草剂活性成分。该研究于2019年2月至5月进行。本研究采用随机区组设计,共7个处理,分别为a(对照)、B(吡唑磺隆、百乐酮)、C(吡唑磺隆、百乐酮)、D(磺胺磺隆、百乐酮)、E(吡唑磺隆、百乐酮)、F(吡唑磺隆、百乐酮)、G(磺胺磺隆、百乐酮)。每次治疗重复4次。数据分析采用方差分析(ANOVA),进一步检验采用5%水平的DMRT检验。结果表明,用磺胺曲酮、吡唑磺隆乙基、氟虫酮等除草剂对研究区紫花苜蓿品种和坎顿苜蓿品种均有抑制杂草生长的作用。以磺胺曲酮为有效成分的除草剂防治效果最好。长豆品种以磺胺酮除草剂处理效果最好。施用含活性氟氧芬的除草剂,没有产生植物毒性症状,但降低了坎顿和培尔顿品种的产量。
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引用次数: 3
Ketahanan tiga klon jahe (Zingiber officinale Rosc.) terhadap penyakit layu bakteri (Ralstonia solanacearum)
Pub Date : 2020-07-14 DOI: 10.19184/jptt.v1i2.18013
Yulia Fista Fauzia, Suhartiningsih Dwi Nurcahyanti
Ginger (Zingiber officinale Rosc.) Is one type of rhizome plant commonly used as a medicinal plant or spice. The rhizome is aromatic and has a distinctive odor used by the community to be used as spices, cooking ingredients and medicinal sources. So far the results of ginger production have not been able to meet people's consumption needs due to a decrease in ginger production at the main ginger development center (West Java). One of the main constraints of ginger cultivation is the attack of bacterial wilt disease caused by the bacterium R. solanacearum. One effort that can be done in tackling bacterial wilt in ginger plants is by using clones that are resistant to bacterial wilt disease of R. solanacearum. The experimental design was carried out using factorial Completely Randomized Design, using ginger clones and the application of R. solanacearum bacteria. This design consists of 6 treatments and 4 replications. Each replication unit consists of 4 plants, so the total number of plants used is 96 plants. The experimental results were analyzed using regression correlation analysis on the parameters of disease progression, while on plant growth parameters using variance analysis and if there were significant differences then continued with Duncan Multiple Range Test (DMRT) with a confidence level of 95%. The results showed red ginger clones including rather resistant criteria with a severity value of 25% while ginger empirical clones and elephant ginger clones were critically vulnerable with 75% severity values and 85.42%. Red ginger clones are more responsive to the infection of R. solanacearum bacteria so that the highest phenol content increases compared to other clones of 0.297 mg/ml, the phenol compounds that play a role in increasing plant resistance. In the variable growth of ginger emprit clone plants have a higher plant height and number of leaves compared to other clones, namely 43.83 cm and 15.44.
姜(生姜)是一种根茎植物,通常用作药用植物或香料。根茎是芳香的,有一种独特的气味,被用作香料,烹饪原料和药用来源。由于主要生姜开发中心(西爪哇)的生姜产量减少,到目前为止,生姜生产的结果还不能满足人们的消费需求。生姜栽培的主要制约因素之一是由枯枯菌引起的青枯病的防治。在生姜植物中解决细菌性枯萎病的一种方法是使用对番茄枯萎病具有抗性的无性系。试验设计采用全因子随机设计,以生姜无性系为试验材料,应用茄青霉菌。试验设计包括6个处理,4个重复。每个复制单元由4个植物组成,因此使用的植物总数为96个。对试验结果进行疾病进展参数的回归相关分析,对植物生长参数的方差分析,如果存在显著差异,则继续进行Duncan多元极差检验(DMRT),置信水平为95%。结果表明,红姜无性系具有相当抗性,严重值为25%,而姜经验无性系和象姜无性系具有极度脆弱,严重值分别为75%和85.42%。红姜无性系对红姜细菌的侵染反应较强,最高酚含量为0.297 mg/ml,高于其他无性系,是提高植株抗性的酚类化合物。姜的变异生长中,emprit无性系植株的株高和叶片数分别为43.83 cm和15.44 cm,高于其他无性系。
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引用次数: 2
Seleksi ketahanan beberapa varietas unggul kedelai terhadap serangan hama kepik hijau (Nezara viridula L.) 选择大豆对绿色瓢虫害虫攻击的优胜品种(Nezara viridula L)。
Pub Date : 2020-07-14 DOI: 10.19184/jptt.v1i2.18014
Nanda Faraz Ayu, S. -
Green stink bug (Nezara viridula L.) is a pod-sucking pest that can cause losses of up to 80%. The use of resistant varieties is an initial control effort that can be reduced or prevent losses due to green stink bug attacks on soybean plants. This study aims to determine the resistance of some soybean varieties to the attack of green stink bug (N. viridula). This study used a completely randomized design (CRD) with 5 treatments and 5 replications. The treatment used 5 soybean varieties namely V1 = Dega 1, V2 = Devon 1, V3 = Dena 1, V4 = Deja 1, V5 =. Detap 1. Each experimental unit is covered and invested 5 stink bug nymphs third instar. Based on the results, showed that Deja 1 and Detap 1 varieties are the best varieties to suppress the percentage of attacks and are included in the category of plants resistant to N. viridula, and have a significant affect to productivity higher than other varieties. Trichome density, hardness and skin thickness of pods significantly affect to the percentage of N. viridula attack so that it is included in the mechanism of antixenosis resistance.
绿臭虫(Nezara viridula L.)是一种吸豆荚的害虫,可以造成高达80%的损失。使用抗性品种是一种初步的控制措施,可以减少或防止绿臭虫对大豆植物的攻击造成的损失。本研究旨在测定一些大豆品种对绿臭虫(N. viridula)侵袭的抗性。本研究采用完全随机设计(CRD), 5个处理,5个重复。处理采用5个大豆品种,分别为V1 =德加1号、V2 =德文1号、V3 =德纳1号、V4 =德加1号、V5 =德加1号。Detap 1。每个实验单元覆盖并投入3龄臭虫若虫5只。结果表明,Deja 1号和Detap 1号是抑制绿僵菌侵染率最好的品种,属于抗绿僵菌植物类别,对产量的影响显著高于其他品种。荚果的毛密度、硬度和果皮厚度对绿僵菌侵染率有显著影响,因而被纳入抗虫机制。
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引用次数: 0
Keragaman arthropoda herbivora dan musuh alami pada tanaman padi lahan rawa di Rowopulo Kecamatan Gumukmas Kabupaten Jember
Pub Date : 2019-03-18 DOI: 10.19184/jptt.v1i1.15586
Muflih Adnan, W. Wagiyana
Rowopulo is one of the swamps in Gumukmas, Jember Regency with its unique soil in the form of peat soil that floats on water and by the community used for rice cultivation, whose production is influenced by the presence of Plant Pests, which is dominated by the Herbivod Arthropods. The presence of herbivorous Arthropods in the swampland rice ecosystem can be suppressed by the presence of useful Arthropod populations such as predators, parasitoids, and pollinators. Related to this, a study was conducted to find out the diversity of Arthropods found in swampy rice plantations. The study began with a land survey, then the determination and marking of observation plots on the map and matching these locations using GPS (Global Positioning System). Observations were made on plots that were made based on the extent of rice plants that were still in one stretch and the same irrigation flow. Each plot was observed ten times at 7-day intervals from rice plants aged 14 days after 77 days after planting. Arthropod sampling using sweep net method. Catching arthropods are put into vials of 5 ml size and then identified up to family level, and the diversity, evenness, and wealth index of the species is calculated. The results of research on swamp rice plants contained 37 families. Ten families act as herbivorous Arthropods, 15 families as predators, six families as parasitoids, one family as pollinators, and five families as detritivores. The highest diversity index in plot 1 was 2.95, while the lowest in plot 2 was 2.70, with the medium category for all observation plots. The magnitude of the diversity index is due to the dominance of only a few orders, such as Orthoptera and Diptera; the highest species wealth index in plot 3 with a value of more than 4 in the high category; Evenness index of all observation plots has a value of less than 1 with uneven categories.   Keywords: arthropods, diversity, natural enemies, swamp land
Rowopulo是Jember Regency Gumukmas的沼泽之一,其独特的土壤形式是漂浮在水面上的泥炭土,并被用于水稻种植的社区所使用,其生产受到植物害虫的影响,其中以食草节肢动物为主。沼泽水稻生态系统中草食性节肢动物的存在可以被有用的节肢动物种群(如捕食者、拟寄生物和传粉者)的存在所抑制。与此相关,一项研究是为了找出沼泽水稻种植园中节肢动物的多样性。研究从土地调查开始,然后在地图上确定和标记观测地块,并使用GPS(全球定位系统)对这些位置进行匹配。观察是基于水稻种植的范围,仍然在一个延伸和相同的灌溉流量。每隔7天观察10次,每隔7天从种植后第14天的水稻植株开始观察。节肢动物取样采用扫网法。将捕获的节肢动物装入5毫升大小的小瓶中,然后按科级进行鉴定,计算物种的多样性、均匀性和财富指数。研究结果显示,沼泽稻属植物共37科。10科为食草节肢动物,15科为捕食者,6科为拟寄生物,1科为传粉者,5科为食腐动物。样区1多样性指数最高为2.95,样区2多样性指数最低为2.70,所有样区均为中等。多样性指数的大小主要是由于直翅目和双翅目等少数目占优势;样地3的物种财富指数最高,值大于4,属于高类;所有观测样地均匀度指数均小于1,类别不均匀。关键词:节肢动物;多样性;天敌
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引用次数: 1
Potensi tepung daun sirsak, serai, kenikir, jeruk nipis, dan kluwih terhadap kumbang bubuk kedelai (Callosobruchus analis F.) pada kedelai (Glyxine max L.) dalam simpanan 面粉、柠檬、树胶、葡萄柚、酸橙和克卢瓦,对储存在贮存的大豆(草甘膦酸盐)的大豆(草甘膦酸盐)的潜在淀粉
Pub Date : 2019-02-19 DOI: 10.19184/bip.v2i1.16167
Laily Dwi Dzulhijja, W. Wagiyana, S. Prastowo
This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. analis imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1 g, 2 g, and 3 g; C) Serai leaf flour with doses of 1 g, 2 g, and 3 g; D) kenikir leaf flour with doses of 1 g, 2 g, and 3 g; E) lime leaf flour with doses of 1 g, 2 g, and 3 g; and F) kluwih leaf flour with doses of 1 g, 2 g, and 3 g. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Tukey HSD Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3 g on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. analis the best analyst was the treatment of soursop leaf flour dose 3 g on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3 g at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3 g, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 g. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3 g because it could kill 50% of the population C. analis with a relatively short time were 9 days after treatment.
摘要本研究旨在确定刺果叶粉、柠檬草粉、刺果叶粉、酸橙叶粉和克鲁威叶粉对大豆种子的影响:1)死亡率,2)对种子的伤害,3)对种子重量的收缩,4)大豆种子沉积物中刺果螟成虫外观,5)刺果螟雌卵产卵,6)概率分析LT50。试验采用完全随机设计(CRD)。治疗方法有:A)对照;B) 1 g, 2 g和3 g剂量的酸菜叶粉;C) Serai叶粉,剂量为1 g, 2 g和3 g;D) kenikir叶粉,剂量分别为1 g, 2 g和3 g;E)酸橙叶粉,剂量分别为1g、2g和3g;F) kluwiwith叶子面粉,剂量分别为1g, 2g和3g。每个治疗使用5个重复。对观察到的参数进行“F”检验方差分析,如果有显著差异,以找出治疗效果的差异,在5%的不信任水平下进行Tukey HSD检验。以3 g芒草叶粉处理5 d死亡率最高,达1.80尾;1个月是6.60;2个月共计13.40;3个月共计11.20美元。对产蛋数的观察结果表明,以刺果叶粉3 g处理5天产蛋数最多,达139只,1个月产蛋数119.20只;2个月91.20项;3个月72.20项。影像观察效果最好的是1个月剂量为3 g的苦参叶粉处理132.20尾;而在2个月时,有79个正面。以3 g剂量的芒草叶粉处理种子损伤效果最好,种子损伤率为67.40粒;种子重量减少17.94 g。对Probit LT50的最佳分析结果是用3 g剂量的刺蒺藜叶粉处理,可以杀死50%的种群c,分析时间相对较短,为处理后9天。
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引用次数: 0
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Jurnal Proteksi Tanaman Tropis
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