Pub Date : 2012-01-01DOI: 10.1109/icept.2011.6066815
M. Da-li
Superhydrophobic nickel films were prepared by electrodeposition and electroless deposition without chemical modification.Experimental results reveal that the nickel film fabricated by electrodeposition is characterized with nanocone arrays and only has a contact angle of about 112°.By coupling electroless deposition,as the second step,hemispherically topped nanocones are generated when electroless deposition time amounts 1min and a high contact angle as much as 152.3° is obtained;nickel surface has successfully transformed from hydrophobic to superhydrophobic.
{"title":"Superhydrophobic Nickel Films Fabricated by Electro/Electroless Deposition","authors":"M. Da-li","doi":"10.1109/icept.2011.6066815","DOIUrl":"https://doi.org/10.1109/icept.2011.6066815","url":null,"abstract":"Superhydrophobic nickel films were prepared by electrodeposition and electroless deposition without chemical modification.Experimental results reveal that the nickel film fabricated by electrodeposition is characterized with nanocone arrays and only has a contact angle of about 112°.By coupling electroless deposition,as the second step,hemispherically topped nanocones are generated when electroless deposition time amounts 1min and a high contact angle as much as 152.3° is obtained;nickel surface has successfully transformed from hydrophobic to superhydrophobic.","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82178573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.1142/9789812835673_0001
Rui-liang Jin, R. Liu, Hong Wang, Jian-wen Cao, Shengfeng Xu, L. Chunyu, Y. Xu, H. Wang
The Mycobacterium tuberculosis hisD gene encoded histidinol dehydrogenase(MtHDH) was amplified by PCR from Mycobacterium tuberculosis H37Rv strain genomic DNA and cloned into a prokaryotic expression vector pET-28a(+) to construct the recombinant expression plasmid pET-28a-HDH.Then this recombinant plasmid was transformed into the strain E.coli BL 21(DE3) and highly expressed after induction with IPTG.Purified MtHDH can catalyse L-histidinol to the corresponding amino acid L-histidine.The optimal pH and temperature of the MtHDH were 8.3 and 45 ℃,respectively.The specific activity of MtHDH was 1.788 U/mg and the relative activity was promoted in the presence of Mn2+,Ca2+,Zn2+ and Co2+.The kinetic constants was determined: Km for NAD+ was found to be 0.9765 mmol/L and for histidinol 2.755 μmol/L.Circular dichroism studies on the MtHDH indicated that the secondary structure of the recombinant protein had about 20.5% α-helix,40.9%β-sheet,4.2%β-turn,34.3% random coil at 25 ℃.
{"title":"Cloning and expression of the histidinol dehydrogenase gene from mycobacterium tuberculosis H37Rv and properties of the recombinant histidinol dehydrogenase","authors":"Rui-liang Jin, R. Liu, Hong Wang, Jian-wen Cao, Shengfeng Xu, L. Chunyu, Y. Xu, H. Wang","doi":"10.1142/9789812835673_0001","DOIUrl":"https://doi.org/10.1142/9789812835673_0001","url":null,"abstract":"The Mycobacterium tuberculosis hisD gene encoded histidinol dehydrogenase(MtHDH) was amplified by PCR from Mycobacterium tuberculosis H37Rv strain genomic DNA and cloned into a prokaryotic expression vector pET-28a(+) to construct the recombinant expression plasmid pET-28a-HDH.Then this recombinant plasmid was transformed into the strain E.coli BL 21(DE3) and highly expressed after induction with IPTG.Purified MtHDH can catalyse L-histidinol to the corresponding amino acid L-histidine.The optimal pH and temperature of the MtHDH were 8.3 and 45 ℃,respectively.The specific activity of MtHDH was 1.788 U/mg and the relative activity was promoted in the presence of Mn2+,Ca2+,Zn2+ and Co2+.The kinetic constants was determined: Km for NAD+ was found to be 0.9765 mmol/L and for histidinol 2.755 μmol/L.Circular dichroism studies on the MtHDH indicated that the secondary structure of the recombinant protein had about 20.5% α-helix,40.9%β-sheet,4.2%β-turn,34.3% random coil at 25 ℃.","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"89 1","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80226387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2007-01-01DOI: 10.1515/energyo.0072.00023
Lin Jian-ming
{"title":"Progress on the Electrolytes for Dye-sensitized Solar Cells","authors":"Lin Jian-ming","doi":"10.1515/energyo.0072.00023","DOIUrl":"https://doi.org/10.1515/energyo.0072.00023","url":null,"abstract":"","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80363257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：