Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.871
Oppong-Kusi Benard Kwame
In the Dormaa traditional area (DTA), the majority of the lands are “stool lands” under the customary tenure system. These land administration sometimes overlaps in unclear land ownership and documentation with the statutory land tenure system. These overlapping can lead to land conflicts and a threat to food security. This Paper seek to better understand why people go to traditional Alternative Dispute Resolution (ADR) court for land conflict and what happen at the court. The study is based on semi-structured interviews and ADR court cases analysis. The result clearly shows people go to traditional ADR court due to land trespassing, unclear land ownership and traditional inheritance system. Oral tradition sometimes changes and traditional landmarks disappear causing confusion. The traditional ADR are favored by locals because these places are easily accessible, flexible, and less expensive. The Chief’s palace which serve as court also serve as home for the community and easy for disputants to access. Disputants were mostly private individuals although traditional leadership sometimes was involved. The plaintiffs tended to lose due to little understanding about the traditional land laws. This paper recommends the creation of more alternative dispute centers and merging the traditional ADR with statutory ADR.
{"title":"Traditional Land Governance and Land Conflict Resolutions: The Case of Dormaa Traditional Area, Ghana","authors":"Oppong-Kusi Benard Kwame","doi":"10.33422/2nd.icmets.2019.12.871","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.871","url":null,"abstract":"In the Dormaa traditional area (DTA), the majority of the lands are “stool lands” under the customary tenure system. These land administration sometimes overlaps in unclear land ownership and documentation with the statutory land tenure system. These overlapping can lead to land conflicts and a threat to food security. This Paper seek to better understand why people go to traditional Alternative Dispute Resolution (ADR) court for land conflict and what happen at the court. The study is based on semi-structured interviews and ADR court cases analysis. The result clearly shows people go to traditional ADR court due to land trespassing, unclear land ownership and traditional inheritance system. Oral tradition sometimes changes and traditional landmarks disappear causing confusion. The traditional ADR are favored by locals because these places are easily accessible, flexible, and less expensive. The Chief’s palace which serve as court also serve as home for the community and easy for disputants to access. Disputants were mostly private individuals although traditional leadership sometimes was involved. The plaintiffs tended to lose due to little understanding about the traditional land laws. This paper recommends the creation of more alternative dispute centers and merging the traditional ADR with statutory ADR.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"539 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124255552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.869
M. Zulfikar
The challenges facing global society this century are environmental problems, one of which is caused by waste. Based on this, Indonesia should make construction waste a serious concern and on the other hand Indonesia must continue its development activities, especially construction in ways that pay attention to the environment in order to provide space for decent living for future generations. A blueprint for the construction sector was drawn up as a grand design and grand strategy called Indonesian Construction 2030. One of the proposed agendas was to promote sustainable construction through a green construction method aimed at restoring and maintaining environmental balance. However, the implementation of green construction which is still limited and has not yet reached the system level is an issue that needs attention and the most crucial part of green construction is that the effect on the environment, especially construction waste, is unclear. The purpose of this study is to develop a conceptual model of the relationship between green construction and reduction of construction waste. The methodology used in this study is intensive literature review. The results of this study will be used to develop a conceptual model of the relationship between the application of green construction to the reduction of construction waste. The results of this study show a dynamic system-based conceptual model of the relationship between the factors of the application of green construction to the reduction of construction waste. This research can also provide insights related to the factors of green construction that can significantly reduce construction waste. Can be an important input for all parties to encourage and improve the application of the concept of sustainable development, especially the green construction method.
{"title":"Conceptual Model of Green Construction toward Construction waste Reduction(A System Dynamic-Based Concept)","authors":"M. Zulfikar","doi":"10.33422/2nd.icmets.2019.12.869","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.869","url":null,"abstract":"The challenges facing global society this century are environmental problems, one of which is caused by waste. Based on this, Indonesia should make construction waste a serious concern and on the other hand Indonesia must continue its development activities, especially construction in ways that pay attention to the environment in order to provide space for decent living for future generations. A blueprint for the construction sector was drawn up as a grand design and grand strategy called Indonesian Construction 2030. One of the proposed agendas was to promote sustainable construction through a green construction method aimed at restoring and maintaining environmental balance. However, the implementation of green construction which is still limited and has not yet reached the system level is an issue that needs attention and the most crucial part of green construction is that the effect on the environment, especially construction waste, is unclear. The purpose of this study is to develop a conceptual model of the relationship between green construction and reduction of construction waste. The methodology used in this study is intensive literature review. The results of this study will be used to develop a conceptual model of the relationship between the application of green construction to the reduction of construction waste. The results of this study show a dynamic system-based conceptual model of the relationship between the factors of the application of green construction to the reduction of construction waste. This research can also provide insights related to the factors of green construction that can significantly reduce construction waste. Can be an important input for all parties to encourage and improve the application of the concept of sustainable development, especially the green construction method.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"273 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132234448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.870
W. Chatasuwatjananon, C. Phombut, S. Rooppakhun
This study aims to compare the spatiotemporal pattern and kinematic data analysis of knee joint during gait between the normal knee and total knee arthroplasty (TKA) using three-dimension (3D) motion technique. A total of 10 subjects consisted of five normal knees and five total knee arthroplasty (TKA) was performed with the average body mass index (BMI) of 23.92±0.40 kg (m 2 ) 1 and 23.81±0.80 kg (m 2 ) 1 , respectively. The 3D technical analysis using software included Qualisys Track Manager (QTM) and Visual 3D were used to create the skeleton model for the analysis of the spatiotemporal and kinematic data. The measurement data were obtained and then compared between groups using statistical mean different analyses. According to the results of the spatiotemporal pattern, there was no statistically significant difference (P > 0.05) between the normal knee and TKA group except the speed, step time and stance time. For the kinematic analysis, the maximum average value of the flexion-extension angle in the normal knee and the TKA were 46 deg occurred in the swing phase at 73 percent of the gait cycle. In the standing phase, the TKA group displayed a slight change of flexion-extension angle than the normal knee. In addition, the maximum average value of angular velocity in the normal knee displayed higher than TKA during standing to swing phase. The results of this study provided basic information and useful for designing and various treatment of knee joints symptoms as well as the physical treatment and sports science.
{"title":"A Comparative Study of Spatiotemporal Pattern and Kinematics of Human Gait between Normal Knee and Total Knee Arthroplasty","authors":"W. Chatasuwatjananon, C. Phombut, S. Rooppakhun","doi":"10.33422/2nd.icmets.2019.12.870","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.870","url":null,"abstract":"This study aims to compare the spatiotemporal pattern and kinematic data analysis of knee joint during gait between the normal knee and total knee arthroplasty (TKA) using three-dimension (3D) motion technique. A total of 10 subjects consisted of five normal knees and five total knee arthroplasty (TKA) was performed with the average body mass index (BMI) of 23.92±0.40 kg (m 2 ) 1 and 23.81±0.80 kg (m 2 ) 1 , respectively. The 3D technical analysis using software included Qualisys Track Manager (QTM) and Visual 3D were used to create the skeleton model for the analysis of the spatiotemporal and kinematic data. The measurement data were obtained and then compared between groups using statistical mean different analyses. According to the results of the spatiotemporal pattern, there was no statistically significant difference (P > 0.05) between the normal knee and TKA group except the speed, step time and stance time. For the kinematic analysis, the maximum average value of the flexion-extension angle in the normal knee and the TKA were 46 deg occurred in the swing phase at 73 percent of the gait cycle. In the standing phase, the TKA group displayed a slight change of flexion-extension angle than the normal knee. In addition, the maximum average value of angular velocity in the normal knee displayed higher than TKA during standing to swing phase. The results of this study provided basic information and useful for designing and various treatment of knee joints symptoms as well as the physical treatment and sports science.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114709901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.877
A. Hubatová-Vacková, D. Lizoňová, A. Pittermannová, V. Tokárová, O. Kašpar, F. Štěpánek
: Microparticles introduced in this work are made of hydrophilic polymers with the ability to incorporate various functional components such as magnetic nanoparticles, liposomes, or enzymes [1, 2]. Microfluidics allows us to fabricate such microparticles in sizes comparable to blood cells, which makes them suitable for drug delivery [3]. The advantage is that these microparticles can be used as tiny chemical or biochemical reactors to locally produce and release pharmaceutically active substances, which are highly reactive or unstable, and therefore cannot be delivered using conventional drug formulations. Another advantage of such system is the possibility of active targeting using surface modification by specific ligands. The goal of this project was to prepare, test and analyse alginate particles, starting by encapsulating a model fluorescent dye and aiming to prepare microparticles able to deliver an antimicrobial compound. Therefore, the methods of preparation of hydrogel microparticles, more specifically microfluidics and microencapsulation, as well as the encapsulation of active compounds within liposomes will be presented in this work. Methods of controlled release are studied, in particular the delivery of doxycycline and the on-demand production of resorufin by the reaction of resazurin with ascorbic acid.
{"title":"Composite alginate microparticles for controlled drug release","authors":"A. Hubatová-Vacková, D. Lizoňová, A. Pittermannová, V. Tokárová, O. Kašpar, F. Štěpánek","doi":"10.33422/2nd.icmets.2019.12.877","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.877","url":null,"abstract":": Microparticles introduced in this work are made of hydrophilic polymers with the ability to incorporate various functional components such as magnetic nanoparticles, liposomes, or enzymes [1, 2]. Microfluidics allows us to fabricate such microparticles in sizes comparable to blood cells, which makes them suitable for drug delivery [3]. The advantage is that these microparticles can be used as tiny chemical or biochemical reactors to locally produce and release pharmaceutically active substances, which are highly reactive or unstable, and therefore cannot be delivered using conventional drug formulations. Another advantage of such system is the possibility of active targeting using surface modification by specific ligands. The goal of this project was to prepare, test and analyse alginate particles, starting by encapsulating a model fluorescent dye and aiming to prepare microparticles able to deliver an antimicrobial compound. Therefore, the methods of preparation of hydrogel microparticles, more specifically microfluidics and microencapsulation, as well as the encapsulation of active compounds within liposomes will be presented in this work. Methods of controlled release are studied, in particular the delivery of doxycycline and the on-demand production of resorufin by the reaction of resazurin with ascorbic acid.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122887136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.872
O. Kašpar, K. Korniienko, L. Mašková, P. Janská, V. Tokárová
One of the most important threats to public health in the 21st century is escalating emergence of secondary infections caused by multi-drug resistant (MDR) bacteria. Antibiotics are losing their beneficial effect, and their persistent residues are gradually polluting drinking water, soil and food products. The omnipresence of antibiotics in very low concentration is the main reason behind the increasing number of MDR bacterial strains. This alarming phenomenon has been caused over the years by the widespread availability of antibiotics in western countries, intensive livestock farming and misuse of antibiotics in the public sector. However, the use of active substances to suppress bacterial infection is not restricted exclusively to humans. It has been shown that some natural antibacterial systems are not significantly affected by the risk of bacterial resistance making them vulnerable. One of the most known examples is garlic, where highly potent but unstable allicin is formed only when the inner cellular structure is compromised. This concept makes allicin everlasting bactericide since it is enzymatically produced when needed for a limited amount of time. We propose to employ spray drying and encapsulation techniques to develop polymer-carriers where purified and stabilised enzyme and substrate (alliin) are physically separated into two different types of carriers or in one carrier simultaneously. Additionally, we want to study the influence of polymer composition, molecular weight and crosslinking ratio on the transport of substrate and products and the overall enzymatic activity of the immobilized enzyme.
{"title":"Immobilization of alliinase in polymer microcarriers","authors":"O. Kašpar, K. Korniienko, L. Mašková, P. Janská, V. Tokárová","doi":"10.33422/2nd.icmets.2019.12.872","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.872","url":null,"abstract":"One of the most important threats to public health in the 21st century is escalating emergence of secondary infections caused by multi-drug resistant (MDR) bacteria. Antibiotics are losing their beneficial effect, and their persistent residues are gradually polluting drinking water, soil and food products. The omnipresence of antibiotics in very low concentration is the main reason behind the increasing number of MDR bacterial strains. This alarming phenomenon has been caused over the years by the widespread availability of antibiotics in western countries, intensive livestock farming and misuse of antibiotics in the public sector. However, the use of active substances to suppress bacterial infection is not restricted exclusively to humans. It has been shown that some natural antibacterial systems are not significantly affected by the risk of bacterial resistance making them vulnerable. One of the most known examples is garlic, where highly potent but unstable allicin is formed only when the inner cellular structure is compromised. This concept makes allicin everlasting bactericide since it is enzymatically produced when needed for a limited amount of time. We propose to employ spray drying and encapsulation techniques to develop polymer-carriers where purified and stabilised enzyme and substrate (alliin) are physically separated into two different types of carriers or in one carrier simultaneously. Additionally, we want to study the influence of polymer composition, molecular weight and crosslinking ratio on the transport of substrate and products and the overall enzymatic activity of the immobilized enzyme.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114929042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.876
V. Tokárová, Hazal Aliye Koyuncu, O. Kašpar
Nanoparticles for biomedical applications need to meet several requirements. Among the most critical ones belong their size and morphology; the surface is modified with specific markers to target specific tissue; and they have a specific function, e.g. drug delivery, sensor, detection or heat ability in hyperthermia treatment Microfluidic synthesis is promising many advantages over standard batch synthesis. Firstly, a large surface area to volume ratio of microchannels helps to increase mass and heat transfer in the system. It provides higher efficiency using smaller reaction volumes than batch methods. Secondly, microchips are more suitable to work at harsh conditions in comparison to the batch reactors with regards to rapid temperature and pressure changes while using toxic and explosive materials. The presented work describes a microfluidic approach to nanoparticles synthesis, namely silver and magnetite nanoparticles. Nanoparticles are synthesized in a droplet-based microfluidic chip with high control over the reaction and process parameters. We employed CFD simulation of two-phase flow in order to design a microfluidic chip and study governing physical parameters and their influence on the droplet formation process and mixing efficiency.
{"title":"Microfluidic approach to nanoparticle synthesis","authors":"V. Tokárová, Hazal Aliye Koyuncu, O. Kašpar","doi":"10.33422/2nd.icmets.2019.12.876","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.876","url":null,"abstract":"Nanoparticles for biomedical applications need to meet several requirements. Among the most critical ones belong their size and morphology; the surface is modified with specific markers to target specific tissue; and they have a specific function, e.g. drug delivery, sensor, detection or heat ability in hyperthermia treatment Microfluidic synthesis is promising many advantages over standard batch synthesis. Firstly, a large surface area to volume ratio of microchannels helps to increase mass and heat transfer in the system. It provides higher efficiency using smaller reaction volumes than batch methods. Secondly, microchips are more suitable to work at harsh conditions in comparison to the batch reactors with regards to rapid temperature and pressure changes while using toxic and explosive materials. The presented work describes a microfluidic approach to nanoparticles synthesis, namely silver and magnetite nanoparticles. Nanoparticles are synthesized in a droplet-based microfluidic chip with high control over the reaction and process parameters. We employed CFD simulation of two-phase flow in order to design a microfluidic chip and study governing physical parameters and their influence on the droplet formation process and mixing efficiency.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129703507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.873
L. Mašková, P. Janská, V. Klimša, V. Tokárová, O. Kašpar
Bacterial resistance towards common antibiotics is considered a major threat to public health. Thereby, new alternative approaches to the treatment of bacterial illnesses are subjects of scientific inquiry. Our research is focused on mimicking a defense mechanism present in a garlic cell that allows swift and effective response to a bacterial attack without further accumulation of the antibacterial ingredient, allicin, in the environment thanks to its short half-life. Allicin is produced via alliinase-catalyzed transformation of alliin that can only occur in the case of the bacterial attack during which a cellular membrane separating the enzyme and its substrate is disintegrated. As our goal is to imitate this process in a drug product, the encapsulation of alliinase into a carrier that will take a role of the membrane barrier is the first step of our work. As alliinase is a protein inclining to degradation under various conditions, spray drying is considered a very suitable method for its encapsulation. In this work, various process parameters were investigated in order to optimize this process in terms of achieving the highest activity of encapsulated alliinase. The effect of feed composition on the rate of allicin’s release was also investigated.
{"title":"Preparation of artificial garlic cell by spray drying process","authors":"L. Mašková, P. Janská, V. Klimša, V. Tokárová, O. Kašpar","doi":"10.33422/2nd.icmets.2019.12.873","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.873","url":null,"abstract":"Bacterial resistance towards common antibiotics is considered a major threat to public health. Thereby, new alternative approaches to the treatment of bacterial illnesses are subjects of scientific inquiry. Our research is focused on mimicking a defense mechanism present in a garlic cell that allows swift and effective response to a bacterial attack without further accumulation of the antibacterial ingredient, allicin, in the environment thanks to its short half-life. Allicin is produced via alliinase-catalyzed transformation of alliin that can only occur in the case of the bacterial attack during which a cellular membrane separating the enzyme and its substrate is disintegrated. As our goal is to imitate this process in a drug product, the encapsulation of alliinase into a carrier that will take a role of the membrane barrier is the first step of our work. As alliinase is a protein inclining to degradation under various conditions, spray drying is considered a very suitable method for its encapsulation. In this work, various process parameters were investigated in order to optimize this process in terms of achieving the highest activity of encapsulated alliinase. The effect of feed composition on the rate of allicin’s release was also investigated.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121939267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-06DOI: 10.33422/2nd.icmets.2019.12.874
S. Priatni, Amelia Adinda, Efendi Oulan, W. Kosasih
Protein hydrolysate from kidney bean ( Phaseolus L. vulgaris ) has been prepared by enzymatic hydrolysis process using papain enzyme. Evaluation of the extent of protein hydrolysis was conducted by measuring the degree of hydrolysis (DH). The optimization of protein hydrolysate production has been carried out by analyzing the influences of papain enzyme concentration, temperature, and time of hydrolysis on the degree of hydrolysis (DH) using RSM Design. The optimized product was spray-dried and analyzed the proximate (moisture, lipid, protein) content. The protein hydrolysate powder product was characterized the foaming capacity and stability, and also by FTIR, and DSC methods. The optimum condition of enzymatic hydrolysis of kidney bean protein was obtained by an addition 3.0 % of papain enzyme at 58 o C for 7 hours. The functional groups present in the kidney bean protein hydrolyzate are amine group, amino, carboxyl; C-O, based on the thermal test, there is a peak point of the 7S protein (vicilin) and 11S (legumin), DH of powdered protein hydrolyzate is 6.30% and the liquid hydrolysate is 7.06%. The highest foaming capacity and stability of this product was reached at pH 3.0. DSC analysis of the product showed two peaks (Tm) at 66.2 o C and 105.6 o C.
{"title":"Production and Characterization of Spray Dried Protein Hydrolysate from Kidney Bean ( (Phaseolus L. Vulgaris) ) Prepared by Enzymatic Hydrolysis","authors":"S. Priatni, Amelia Adinda, Efendi Oulan, W. Kosasih","doi":"10.33422/2nd.icmets.2019.12.874","DOIUrl":"https://doi.org/10.33422/2nd.icmets.2019.12.874","url":null,"abstract":"Protein hydrolysate from kidney bean ( Phaseolus L. vulgaris ) has been prepared by enzymatic hydrolysis process using papain enzyme. Evaluation of the extent of protein hydrolysis was conducted by measuring the degree of hydrolysis (DH). The optimization of protein hydrolysate production has been carried out by analyzing the influences of papain enzyme concentration, temperature, and time of hydrolysis on the degree of hydrolysis (DH) using RSM Design. The optimized product was spray-dried and analyzed the proximate (moisture, lipid, protein) content. The protein hydrolysate powder product was characterized the foaming capacity and stability, and also by FTIR, and DSC methods. The optimum condition of enzymatic hydrolysis of kidney bean protein was obtained by an addition 3.0 % of papain enzyme at 58 o C for 7 hours. The functional groups present in the kidney bean protein hydrolyzate are amine group, amino, carboxyl; C-O, based on the thermal test, there is a peak point of the 7S protein (vicilin) and 11S (legumin), DH of powdered protein hydrolyzate is 6.30% and the liquid hydrolysate is 7.06%. The highest foaming capacity and stability of this product was reached at pH 3.0. DSC analysis of the product showed two peaks (Tm) at 66.2 o C and 105.6 o C.","PeriodicalId":170442,"journal":{"name":"Proceedings of The 2nd International Conference on Modern research in Engineering, Technology and Science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127012461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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