Pub Date : 2012-01-01DOI: 10.6084/M9.FIGSHARE.1284172.V6
A. Asbahi, M. A. A. Maqtari, Khalid Mohammed Naji
The ability of plants to tolerate drought conditions is crucial for plant survival and crop production worldwide. The present data confirm previous findings reported existence of a strong relation between abscisic acid (ABA) content and amino acid accumulation as response water stress which is one of the most important defense mechanism activated during water stress in many plant species. Therefore, free amino acids were measured to determine any changes in the metabolite pool in relation to ABA content. The ABA defective mutants of Arabidopsis plants were subjected to leaf dehydration for Arabidopsis on Whatman 3 mm filter paper at room temperature while, tomato mutant plants were subjected to drought stresses for tomato plants by withholding water. To understand the signal transduction mechanisms underlying osmotic stress-regulating gene induction and activation of osmoprotectant free amino acid synthesizing genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in ABA biosynthesis under drought stress conditions. The present results revealed an accumulation of specific free amino acid in water stressed tissues in which majority of free amino acids are increased especially those playing an osmoprotectant role such as proline and glycine. Drought stress related Amino acids contents are significantly reduced in the mutants under water stress condition while they are increased significantly in the wild types plants. The exhibited higher accumulation of other amino acids under stressed condition in the mutant plants suggest that, their expressions are regulated in an ABA independent pathways. In addition, free amino acids content changes during water stress condition suggest their contribution in drought toleration as common compatible osmolytes.
{"title":"ABA biosynthesis defective mutants reduce some free amino acids accumulation under drought stress in tomato leaves in comparison with Arabidopsis plants tissues","authors":"A. Asbahi, M. A. A. Maqtari, Khalid Mohammed Naji","doi":"10.6084/M9.FIGSHARE.1284172.V6","DOIUrl":"https://doi.org/10.6084/M9.FIGSHARE.1284172.V6","url":null,"abstract":"The ability of plants to tolerate drought conditions is crucial for plant survival and crop production worldwide. The present data confirm previous findings reported existence of a strong relation between abscisic acid (ABA) content and amino acid accumulation as response water stress which is one of the most important defense mechanism activated during water stress in many plant species. Therefore, free amino acids were measured to determine any changes in the metabolite pool in relation to ABA content. The ABA defective mutants of Arabidopsis plants were subjected to leaf dehydration for Arabidopsis on Whatman 3 mm filter paper at room temperature while, tomato mutant plants were subjected to drought stresses for tomato plants by withholding water. To understand the signal transduction mechanisms underlying osmotic stress-regulating gene induction and activation of osmoprotectant free amino acid synthesizing genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in ABA biosynthesis under drought stress conditions. The present results revealed an accumulation of specific free amino acid in water stressed tissues in which majority of free amino acids are increased especially those playing an osmoprotectant role such as proline and glycine. Drought stress related Amino acids contents are significantly reduced in the mutants under water stress condition while they are increased significantly in the wild types plants. The exhibited higher accumulation of other amino acids under stressed condition in the mutant plants suggest that, their expressions are regulated in an ABA independent pathways. In addition, free amino acids content changes during water stress condition suggest their contribution in drought toleration as common compatible osmolytes.","PeriodicalId":17161,"journal":{"name":"Journal of Stress Physiology & Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71206219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dur um wheat (Triticum durum Desf.) has been extensively cultivated in Algeria for many centuries. During this long period, the species encountered a large diversification implied by the great diversity of climates that led to great genetic diversity of the species. The purpose of this study is to improve the management of phytogenetic resources that can serve as potential breeders for the amelioration of wheat. The study aims at evaluating the diversity of 1019 accessions of durum wheat from different regions of Algeria and which are stored at the Constantine ITGC. The analysis of the results concerning phenological and morphophysiological characters revealed an important intra and intervarietal genetic variability. Subsequently it appeared that the 1019 accessions belong to 19 botanic varieties that differ mainly by the cob, silk and grain colours. Among the characters involved in this study, some appeared to have a direct connection with the adaptation to water stress and thus allowed us identifying the most resistant varieties.
杜尔小麦(Triticum durum Desf.)在阿尔及利亚被广泛种植了许多世纪。在这个漫长的时期，物种经历了巨大的多样化，这是由巨大的气候多样性所暗示的，这导致了物种的巨大遗传多样性。本研究的目的是改进植物遗传资源的管理，为小麦改良提供潜在的育种资源。该研究旨在评估储存在Constantine ITGC的来自阿尔及利亚不同地区的1019种硬粒小麦的多样性。物候和形态生理性状分析结果表明，品种内和品种间具有重要的遗传变异。随后发现，这1019份材料属于19个植物品种，主要区别在于穗轴、丝和籽粒颜色。在本研究涉及的性状中，有些似乎与适应水分胁迫有直接联系，从而使我们能够确定最抗性的品种。
{"title":"Evaluation of genetic diversity of an Algerian durum wheat (triticum durum desf.) collection.","authors":"B. Leila, Gherroucha Hocine, Boukaboub Amar, Bouchtab Karima, Baka. Mébarek, Samra Kheireddine","doi":"10.5251/ABJNA.2011.2.7.1126.1134","DOIUrl":"https://doi.org/10.5251/ABJNA.2011.2.7.1126.1134","url":null,"abstract":"Dur um wheat (Triticum durum Desf.) has been extensively cultivated in Algeria for many centuries. During this long period, the species encountered a large diversification implied by the great diversity of climates that led to great genetic diversity of the species. The purpose of this study is to improve the management of phytogenetic resources that can serve as potential breeders for the amelioration of wheat. The study aims at evaluating the diversity of 1019 accessions of durum wheat from different regions of Algeria and which are stored at the Constantine ITGC. The analysis of the results concerning phenological and morphophysiological characters revealed an important intra and intervarietal genetic variability. Subsequently it appeared that the 1019 accessions belong to 19 botanic varieties that differ mainly by the cob, silk and grain colours. Among the characters involved in this study, some appeared to have a direct connection with the adaptation to water stress and thus allowed us identifying the most resistant varieties.","PeriodicalId":17161,"journal":{"name":"Journal of Stress Physiology & Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71084787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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