S. Sunita, V. Chaturvedi, P. Gupta, T. G. Sumithra, U. Laxmi
Anthrax is an often fatal, bacterial infection affecting various animal species which also has serious impact on human health. Major virulence factors produced by different strains of Bacillus anthracis include polyglutamic acid capsule and three-component toxin. Nevertheless, involvement of other pathological features cannot be overruled. Therefore, the present study was planned to determine, production of extracellular DNAse by B. anthracis for the first time and was compared with other principal bacterial species that have already been reported to produce the extracellular DNAse. Two distinct DNAse activity assays namely plate and tube assays were used to demonstrate DNAse activity. The B. anthracis strains used in the study showed DNAse production in both the assays, however further intensive research is required to understand its role in the pathogenesis of anthrax and its possible mechanism of action.
{"title":"Demonstration of Deoxyribonuclease Activity for Bacillus anthracis and its Comparison with other DNAse Producing Bacteria","authors":"S. Sunita, V. Chaturvedi, P. Gupta, T. G. Sumithra, U. Laxmi","doi":"10.2016/JVPH.V13I1.212","DOIUrl":"https://doi.org/10.2016/JVPH.V13I1.212","url":null,"abstract":"Anthrax is an often fatal, bacterial infection affecting various animal species which also has serious impact on human health. Major virulence factors produced by different strains of Bacillus anthracis include polyglutamic acid capsule and three-component toxin. Nevertheless, involvement of other pathological features cannot be overruled. Therefore, the present study was planned to determine, production of extracellular DNAse by B. anthracis for the first time and was compared with other principal bacterial species that have already been reported to produce the extracellular DNAse. Two distinct DNAse activity assays namely plate and tube assays were used to demonstrate DNAse activity. The B. anthracis strains used in the study showed DNAse production in both the assays, however further intensive research is required to understand its role in the pathogenesis of anthrax and its possible mechanism of action.","PeriodicalId":17487,"journal":{"name":"Journal of Veterinary Public Health","volume":"61 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83611379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. R. Patil, R. Zende, A. Paturkar, V. S. W. A. M. Netake
A multiresidue method was standardized using Gas Chromatography (GC) equipped with Electron Capture Detector (GC-ECD) for simultaneous detection and quantification of selected pesticide residues viz., DDT isomers, HCH isomers and α-endosulphan in chicken egg samples. Method validation revealed average recoveries ranging between 68.64% to 115.62% with percent RSD below 10% for all the analytes under study. Among all the 120 chicken egg samples analysed from five different sources, 76 (63.33%), 17 (14.16%) and 8 (6.66%) samples showed presence of DDT isomers, HCH isomers and α-endosulphan, respectively. Highest prevalence of pesticide residues was noted for DDT isomers (70.83% each) in eggs collected from northern Maharashtra and Andhra Pradesh followed by HCH isomers from western Maharashtra (25%) and for α- endosulphan from Karnataka and Tamil Nadu (12.5%). On ther hand, lowest prevalence was observed for DDT isomers (54.16%) in eggs from Western Maharashtra, followed by HCH isomers from Andhra Pradesh (8.33%) and nil for α-endosulphan from northern and western Maharashtra. Although the eggs marketed in the Mumbai city were containing organochlorine pesticide residues, none of the egg sample showed presence of residue levels in excess of permissible levels prescribed by PFA and Codex.
{"title":"Screening of Organochlorine Pesticide Residues in Chicken Eggs in and around Mumbai City","authors":"A. R. Patil, R. Zende, A. Paturkar, V. S. W. A. M. Netake","doi":"10.2016/JVPH.V12I2.97","DOIUrl":"https://doi.org/10.2016/JVPH.V12I2.97","url":null,"abstract":"A multiresidue method was standardized using Gas Chromatography (GC) equipped with Electron Capture Detector (GC-ECD) for simultaneous detection and quantification of selected pesticide residues viz., DDT isomers, HCH isomers and α-endosulphan in chicken egg samples. Method validation revealed average recoveries ranging between 68.64% to 115.62% with percent RSD below 10% for all the analytes under study. Among all the 120 chicken egg samples analysed from five different sources, 76 (63.33%), 17 (14.16%) and 8 (6.66%) samples showed presence of DDT isomers, HCH isomers and α-endosulphan, respectively. Highest prevalence of pesticide residues was noted for DDT isomers (70.83% each) in eggs collected from northern Maharashtra and Andhra Pradesh followed by HCH isomers from western Maharashtra (25%) and for α- endosulphan from Karnataka and Tamil Nadu (12.5%). On ther hand, lowest prevalence was observed for DDT isomers (54.16%) in eggs from Western Maharashtra, followed by HCH isomers from Andhra Pradesh (8.33%) and nil for α-endosulphan from northern and western Maharashtra. Although the eggs marketed in the Mumbai city were containing organochlorine pesticide residues, none of the egg sample showed presence of residue levels in excess of permissible levels prescribed by PFA and Codex.","PeriodicalId":17487,"journal":{"name":"Journal of Veterinary Public Health","volume":"2 1","pages":"81-84"},"PeriodicalIF":0.0,"publicationDate":"2016-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82278955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
; "> Pandemic group of Vibrio parahaemolyticus possesses a variety of ‘unique’ DNA markers, like toxRS /new sequence (GS-PCR), orf-8 , a 930 bp AP-PCR fragment (PGS-PCR) and various other markers. In the present study, initially 94 tdh + and trh - (virulent), then 75 toxR + (speciesspecific), V. parahaemolyticus isolates from saline water fishes of Kolkata Metropolis based fish markets were analyzed for the presence of three pandemic markers viz ., toxRS /new region (GSPCR), orf-8 and PGS-PCR. Out of 94 tdh + isolates, 58 (61.7%) were positive for toxRS/ new region in GS-PCR. None of the isolates were positive for the orf-8 gene; however, in PGS-PCR assay all tested isolates ( tdh + ) were positive. But when 75 toxR + non-virulent isolates were tested by all these three markers, no isolate was found positive for GS-PCR and orf-8 PCR; however, all were found positive in PGS-PCR. Thus, from the present study it may be inferred that only GS-PCR is a suitable marker for identification of pandemic group; while, orf-8 and PGS-PCR assay might not be appropriate.
;副溶血性弧菌大流行组具有多种“独特”的DNA标记，如toxRS /new sequence (GS-PCR)、orf-8、930 bp AP-PCR片段(PGS-PCR)和各种其他标记。本研究首先分析了从加尔各答市鱼市场的咸水鱼中分离出的94株tdh +和trh -(毒力)，然后分析了75株toxR +(种特异性)，分析了毒虫/新区域(GSPCR)、orf-8和PGS-PCR三种大流行标志物的存在。94株tdh +菌株中，58株(61.7%)的GS-PCR检测结果为toxRS/ new region阳性。所有分离株均无orf-8基因阳性;PGS-PCR检测结果显示，所有分离株tdh +阳性。但对75株无毒毒株进行3种标记检测时，均未发现GS-PCR和orf-8 PCR阳性;PGS-PCR结果均为阳性。因此，从本研究可以推断，只有GS-PCR是鉴别大流行群体的合适标记;而orf-8和PGS-PCR检测可能不合适。
{"title":"Comparative analysis of pandemic clone markers of Vibrio parahaemolyticus isolated from saline water fishes in Kolkata.","authors":"Anjay, S. C. Das, Ashok Kumar, S. Malik","doi":"10.2016/JVPH.V9I1.30","DOIUrl":"https://doi.org/10.2016/JVPH.V9I1.30","url":null,"abstract":"; \"> Pandemic group of Vibrio parahaemolyticus possesses a variety of ‘unique’ DNA markers, like toxRS /new sequence (GS-PCR), orf-8 , a 930 bp AP-PCR fragment (PGS-PCR) and various other markers. In the present study, initially 94 tdh + and trh - (virulent), then 75 toxR + (speciesspecific), V. parahaemolyticus isolates from saline water fishes of Kolkata Metropolis based fish markets were analyzed for the presence of three pandemic markers viz ., toxRS /new region (GSPCR), orf-8 and PGS-PCR. Out of 94 tdh + isolates, 58 (61.7%) were positive for toxRS/ new region in GS-PCR. None of the isolates were positive for the orf-8 gene; however, in PGS-PCR assay all tested isolates ( tdh + ) were positive. But when 75 toxR + non-virulent isolates were tested by all these three markers, no isolate was found positive for GS-PCR and orf-8 PCR; however, all were found positive in PGS-PCR. Thus, from the present study it may be inferred that only GS-PCR is a suitable marker for identification of pandemic group; while, orf-8 and PGS-PCR assay might not be appropriate.","PeriodicalId":17487,"journal":{"name":"Journal of Veterinary Public Health","volume":"121 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76726073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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