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Plant Cell Culture & Micropropagation最新文献

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In vitro culture of Stryphnodendron adstringens Mart. Coville 马菖蒲的离体培养。阿尔蒙
Pub Date : 2023-01-01 DOI: 10.46526/pccm.2023.v19.192
Gabriela Ferreira Nogueira, Renato Paiva, Daiane Peixoto Vargas, Raírys Cravo Herrera, Tainá Teixeira Rocha, Diogo Pedrosa Correa da Silva
Stryphnodendron adstringens (Mart.) Coville is a species native to Cerrado with high medicinal potential. It is an importanttannin source, is used mainly due to its healing, anti-inflammatory, and anti-septic activities. Due to the difficulties insexual propagation, the objective of this work was to study some aspects of its the in vitro culture. The concentration ofsalts influenced the establishment of the initial explant in vitro, with a higher percentage of germination in the culturemedium without salts. The combination of concentrations of 1.0 mg L-1 BAP and 0.1 mg L-1 NAA promoted greater shootformation, and the concentration of 2.0 mg L -1 IBA promoted greater histogenesis. The composition of the culture medium influenced the establishment and development of S. adstringens. Index terms: Native Brazilian tree; tissue culture; micropropagation; growth regulator
黄连藤(Mart.)科维尔是塞拉多原生物种,具有很高的药用潜力。它是一种重要的单宁来源,主要是因为它具有愈合、抗炎和抗菌活性。由于其无性繁殖的困难,本工作的目的是研究其体外培养的一些方面。盐的浓度影响外植体的体外萌发,在无盐培养基中萌发率较高。1.0 mg L-1 BAP和0.1 mg L-1 NAA的组合能促进组织形成,2.0 mg L-1 IBA的组合能促进组织形成。培养基的组成影响了葡萄球菌的建立和发展。索引项:巴西原生树;组织文化;微体繁殖;生长调节剂
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引用次数: 0
In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators 葡萄离体繁殖和生根:培养基和植物生长调节剂
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2019.v15i1.136
Daniele Camargo Nascimento, M. Dini, Norton Victor Sampaio, Márcia Wulff Schuch
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引用次数: 1
In vitro multiplication of Cedrela fissilis Vell.: A threatened brazilian hardwood forest tree 龙舌兰的离体增殖。一种受威胁的巴西阔叶树
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2021.v17.165
Yasmim Smarsaro Bonfá, Vitor L. Nascimento, E. Werner
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引用次数: 0
Combined effects of cultivar, culture system, cytokinin type and concentration on in vitro regenerative rate of banana 品种、培养体系、细胞分裂素类型和浓度对香蕉离体再生率的综合影响
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2021.v17.164
D. Almeida da Silva, Ramon Felipe Scherer, Daniel Ferreira Holderbaum, Miguel Pedro Guerra, N. Steiner
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引用次数: 0
Triiodobenzoic acid affects the regenerative competence in root explants of Adenocalymma nodosum (Bignoniaceae) 三碘苯甲酸对大戟科腺瘤根外植体再生能力的影响
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2020.v16.146
V. P. Paiva Neto, Janaina Fernanda de Oliveira, Luciana Guimarães Sanches, Monica Cristina Rezende Zuffo Borges, Ana Claudia Ferreira da Cruz, W. Campos Otoni
Adenocalymma nodosum , a native plant species from Brazilian Savanna, has a very interesting propagation mechanism by root segments, mainly, in areas with weed mechanical control or soil prepared by farming equipment such as plough that results in the cited root segments, enabling high regenerative capacity to generate adventitious shoots. This regenerative competence of root segments, without exogenous plant growth regulators, was previously showed to occur on in vitro conditions. Aiming to study the effect of the auxin transport inhibition on the regenerative capacity of the root segments of this species, root explants were placed in different orientations (horizontal, upright and inverted upright) on Murashige and Skoog medium supplemented or not with triiodobenzoic acid (TIBA). It was possible to verify that root explants have a polar response with shoot buds being directly differentiated from pericycle cells only at root proximal ends. Use of TIBA, an auxin transport inhibitor, inhibited organogenesis and induced callogenesis, showing that auxin transport is essential to the favorable hormonal balance to bud differentiation at the explant proximal end.
腺瘤(Adenocalymma nodosum)是一种来自巴西热带稀树草原的本土植物,它的根段繁殖机制非常有趣,主要是在杂草机械控制或犁等耕作设备制备的土壤中产生引用的根段,具有很高的再生能力,可以产生不定芽。这种根段的再生能力,在没有外源植物生长调节剂的情况下,在体外条件下发生。为了研究生长素运输抑制对该物种根段再生能力的影响,将根外植体以不同方向(水平、直立和倒直立)放置在添加或不添加三碘苯甲酸(TIBA)的Murashige和Skoog培养基上。有可能证实根外植体具有极性响应,茎芽仅在根近端直接从中柱鞘细胞分化出来。使用TIBA(一种生长素运输抑制剂)可以抑制器官发生和诱导胼胝质形成,这表明生长素运输对于外植体近端芽分化的有利激素平衡至关重要。
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引用次数: 1
Medicinal and ornamental plant tissue culture in Brazil and worldwide: Researches and patents 药用和观赏植物组织培养在巴西和世界各地:研究和专利
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2019.v15i1.137
Dora dos Santos Costa, Ida Carolina Neves Direito, Cristiane Pimentel Victório
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引用次数: 1
Sucrose concentration alters the physiological potential of in vitro germinated gabiroba seeds 蔗糖浓度改变体外萌发gabiroba种子的生理电位
Pub Date : 1900-01-01 DOI: 10.46526/pccm.2021.v17.167
Geiciane Cintra de Souza, Lana Laene Dias, Ana Claudia Alves D’Abadia, Givanildo Zildo da Silva, Carla Gomes Machado, Edésio Fialho do Reis, Diego Ismael Rocha, Antônio Paulino Costa Netto
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引用次数: 0
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Plant Cell Culture & Micropropagation
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