首页 > 最新文献

International Journal of Scholarly Research in Life Sciences最新文献

英文 中文
Evaluation of genotoxicity of dry powders of pomelo (Citrus maxima) flowers by micronucleus assay 微核法评价柚子花干粉的遗传毒性
Pub Date : 2023-09-30 DOI: 10.56781/ijsrls.2023.2.1.0031
None Chien-Hsiang Ni, None Yun-Xuan Chang, None Tsung-Han Wu, None Ya-Peng Wang, None Chia-Chi Chen, None Tzu-Yun Chi, None Yen-Jung Lu, None Pi-Hsin Chen, None Ya-Ling Cyue, None Shih-Yi Guo, None Suz-Ching Ke, None Yu-Ying Fang, None Szu-Ping Sung, None Chien-Chao Chiu, None Ching-Feng Chiu, None Hsuan-Wen Chiu, None Wei-Huang Tsai, None Yu-Hsing Lin, None Shao-Wen Hung
Plants have been used as traditional medicine or health products for several thousands of years. The present study was aimed to evaluate the genotoxicity of pomelo flower powders by micronucleus assay In vivo. During the In vivo genotoxicity-evaluated experiment, the experimental animal’s clinical behavior, body weight (BW), food consumption, and the percentage of RET/RBCs (reticulocytes/red blood cells) and MN-RET/RETs (micronucleated reticulocytes/reticulocytes) after the treatments of pomelo (Citrus maxima) flower powders were evaluated. Both sexes ICR mice were treated three daily treatments by intraperitoneal injection of 2 mg/kg of mitomycin C (genotoxicity induction) or by oral route of 200 μL of PBS (the normal control group). Until 30th hours after the last treatment, K2-EDTA-anticoagulated peripheral blood specimens were collected. These blood samples were processed for the microscopy-based analysis using Giemsa stain and the percentage of reticulocytes and micronucleated reticulocytes was determined. The results were shown that the experimental animal’s clinical behaviors were normal in all groups. The BW and food consumption were no significant difference between all groups. RET/RBCs (%) in male or female ICR mice in the negative control group, the normal control group, the low dose of pomelo (C. maxima) flower powder group, the middle dose of pomelo (C. maxima) flower powder group, and the high dose of pomelo (C. maxima) flower powder group were respectively 8.8 ± 2.3 / 9.6 ± 2.6, 23.0 ± 2.5 / 22.4 ± 2.3, 23.4 ± 2.1 / 23.2 ± 3.8, 24.2 ± 3.6 / 23.0 ± 1.9, and 21.6 ± 3.2 / 21.6 ± 2.4; MN-RET/RETs (‰) in male or female ICR mice in the negative control group, the normal control group, the low dose of pomelo (C. maxima) flower powders group, the middle dose of pomelo (C. maxima) flower powder group, and the high dose of pomelo (C. maxima) flower powder group were 43.0 ± 12.5 / 39.4 ± 9.8, 2.6 ± 1.5 / 2.6 ± 1.5, 2.4 ± 1.1 / 2.2 ± 1.3, 2.2 ± 1.3 / 2.0 ± 1.2, and 1.8 ± 0.8 / 1.8 ± 0.8, respectively. Both RET/RBCs (%) and MN-RET/RETs (%) in male or female ICR mice in the negative control group were significantly difference than the other groups (p < 0.001). Taken all results together, pomelo (C. maxima) flower powders were without genotoxicity. Therefore, pomelo (C. maxima) flower powders were safety.
几千年来,植物一直被用作传统药物或保健品。本研究旨在通过体内微核试验评价柚子花粉的遗传毒性。在体内基因毒性评价实验中,评价了柚花粉处理后实验动物的临床行为、体重(BW)、食量以及RET/红细胞(网织红细胞/红细胞)和MN-RET/RET(微核网织红细胞/网织红细胞)的百分比。采用2 mg/kg丝裂霉素C(遗传毒性诱导)腹腔注射或200 μL PBS(正常对照组)口服3次。至末次治疗后30h,采集外周血k2 - edta抗凝血标本。用吉姆萨染色法对血样进行显微分析,并测定网织红细胞和微核网织红细胞的百分比。结果显示,各组实验动物临床行为均正常。各组间体重和食量无显著差异。RET /红血球(%)在男性或女性ICR小鼠负对照组,正常对照组、低剂量的柚子花粉组(c .最大值),中间剂量的柚子(c .最大值)花粉组和高剂量的柚子(c .最大值)花粉组分别为8.8±2.3/9.6±2.6,23.0±2.5/22.4±2.3,23.4±2.1/23.2±3.8,24.2±3.6/23.0±1.9,21.6±3.2/21.6±2.4;MN-RET / ret(‰)在男性或女性ICR小鼠负对照组,正常对照组、低剂量的柚子(c .最大值)花粉组,中间剂量的柚子花粉组(c .最大值),和高剂量的柚子(c .最大值)花粉组分别为43.0±12.5/39.4±9.8,2.6±1.5/2.6±1.5,2.4±1.1/2.2±1.3,2.2±1.3/2.0±1.2,1.8±0.8/1.8±0.8,分别。阴性对照组雄性和雌性ICR小鼠的RET/ rbc(%)和MN-RET/RET(%)与其他组比较差异均有统计学意义(p <0.001)。综上所述,柚花粉无遗传毒性。因此,柚花粉是安全的。
{"title":"Evaluation of genotoxicity of dry powders of pomelo (Citrus maxima) flowers by micronucleus assay","authors":"None Chien-Hsiang Ni, None Yun-Xuan Chang, None Tsung-Han Wu, None Ya-Peng Wang, None Chia-Chi Chen, None Tzu-Yun Chi, None Yen-Jung Lu, None Pi-Hsin Chen, None Ya-Ling Cyue, None Shih-Yi Guo, None Suz-Ching Ke, None Yu-Ying Fang, None Szu-Ping Sung, None Chien-Chao Chiu, None Ching-Feng Chiu, None Hsuan-Wen Chiu, None Wei-Huang Tsai, None Yu-Hsing Lin, None Shao-Wen Hung","doi":"10.56781/ijsrls.2023.2.1.0031","DOIUrl":"https://doi.org/10.56781/ijsrls.2023.2.1.0031","url":null,"abstract":"Plants have been used as traditional medicine or health products for several thousands of years. The present study was aimed to evaluate the genotoxicity of pomelo flower powders by micronucleus assay In vivo. During the In vivo genotoxicity-evaluated experiment, the experimental animal’s clinical behavior, body weight (BW), food consumption, and the percentage of RET/RBCs (reticulocytes/red blood cells) and MN-RET/RETs (micronucleated reticulocytes/reticulocytes) after the treatments of pomelo (Citrus maxima) flower powders were evaluated. Both sexes ICR mice were treated three daily treatments by intraperitoneal injection of 2 mg/kg of mitomycin C (genotoxicity induction) or by oral route of 200 μL of PBS (the normal control group). Until 30th hours after the last treatment, K2-EDTA-anticoagulated peripheral blood specimens were collected. These blood samples were processed for the microscopy-based analysis using Giemsa stain and the percentage of reticulocytes and micronucleated reticulocytes was determined. The results were shown that the experimental animal’s clinical behaviors were normal in all groups. The BW and food consumption were no significant difference between all groups. RET/RBCs (%) in male or female ICR mice in the negative control group, the normal control group, the low dose of pomelo (C. maxima) flower powder group, the middle dose of pomelo (C. maxima) flower powder group, and the high dose of pomelo (C. maxima) flower powder group were respectively 8.8 ± 2.3 / 9.6 ± 2.6, 23.0 ± 2.5 / 22.4 ± 2.3, 23.4 ± 2.1 / 23.2 ± 3.8, 24.2 ± 3.6 / 23.0 ± 1.9, and 21.6 ± 3.2 / 21.6 ± 2.4; MN-RET/RETs (‰) in male or female ICR mice in the negative control group, the normal control group, the low dose of pomelo (C. maxima) flower powders group, the middle dose of pomelo (C. maxima) flower powder group, and the high dose of pomelo (C. maxima) flower powder group were 43.0 ± 12.5 / 39.4 ± 9.8, 2.6 ± 1.5 / 2.6 ± 1.5, 2.4 ± 1.1 / 2.2 ± 1.3, 2.2 ± 1.3 / 2.0 ± 1.2, and 1.8 ± 0.8 / 1.8 ± 0.8, respectively. Both RET/RBCs (%) and MN-RET/RETs (%) in male or female ICR mice in the negative control group were significantly difference than the other groups (p < 0.001). Taken all results together, pomelo (C. maxima) flower powders were without genotoxicity. Therefore, pomelo (C. maxima) flower powders were safety.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136271813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dragon fruit (Hylocereus undatus (Haw.) Britt.) powders attenuate ulcerative disease symptoms via dextran sulfate sodium-induced mouse model 火龙果(Hylocereus undatus)(Britt.)粉末通过硫酸葡聚糖钠诱导的小鼠模型减轻溃疡性疾病的症状
Pub Date : 2023-09-30 DOI: 10.56781/ijsrls.2023.2.1.0030
None Yu-Wen Hung, None Chia-Chi Chen, None Yun-Xuan Chang, None Tzu-Yun Chi, None Ya-Peng Wang, None Tsung-Han Wu, None Ya-Ling Cyue, None Pi-Hsin Chen, None Yen-Jung Lu, None Shih-Yi Guo, None Suz-Ching Ke, None Yu-Ying Fang, None Szu-Ping Sung, None Yan-Zhong Wu, None Chien-Chao Chiu, None Ching-Feng Chiu, None Hsuan-Wen Chiu, None Wei-Huang Tsai, None Yu-Hsing Lin, None Shao-Wen Hung
Dragon fruit is cactus based fruit that has loads of health benefits such as lowering risks of a blood sugar spike, aids in digestion etc. Its nutritional content is rich in vitamin C, vitamin E, magnesium, iron etc. Its functions on the physiological regulation are well known. Inflammatory bowel diseases (IBD) are multifactorial chronic intestinal disorders. Currently, mesalamine etc. and therapeutic strategies were suggested for IBD therapy. However, the etiology of IBD remains unclear which is an ongoing challenge and side effects of therapeutic drugs must be also considered. Thus, the aim of this study was evaluated the efficacy and therapeutic strategies investigations on the attenuated IBD symptoms via administrating three doses of dragon fruit powders in the 2% dextran sulfate sodium (DSS)-induced IBD mouse model. The female C57BL/6 mice were divided respectively the normal control group (n = 10), the negative control group (n = 10), three dose groups (n = 10 per group) of dragon fruit powders (250 mg/kg BW, 500 mg/kg BW, and 1,000 mg/kg BW). Exception of the normal control group, other groups were administrated with 2% DSS for 5 days. Later, the normal drinking water was provide to C57BL/6 mice until the end of the experiment. At the end of the experiment, the body weight (BW), the stool appearance/status, the macroscopic and microscopic colonic injuries, and myeloperoxidase (MPO) activity were monitored, measured and scored. The results were showed that BW of C57BL/6 mice in the negative control group, three dragon fruit powder groups was gradually reduced during the IBD period induced by 2% DSS, and BW of C57BL/6 mice gradually increased when the 2% DSS in drinking water was replaced with the normal drinking water. When the experiment was carried out to the 3rd to 4th week, BW of the negative control group was significantly lower than that of the normal control group. The stool appearance/status was presented that stool score in the negative control group was significantly higher than that in the normal control group (p < 0.001). The stool score in the high-dose dragon fruit powder group was significantly lower than that in the negative control group (p < 0.001). The macroscopic colons of C57BL/6 mice were performed at the end of the experiment. (1) Gut weight: It can be seen that gut weight in the normal control group is lowest and the gut weight in the negative control group is highest between all groups. The gut weight in the negative control group was higher than that in the normal control group, medium-dose dragon fruit powder group, and high-dose dragon fruit powder group were seen. (2) Gut length: It can be seen that gut length in the normal control group is longest. The gut length in the normal control group is significantly longer than that in the other groups. Exception of the normal control group, other groups were not significant difference compared to each other. (3) Gut weight-to-gut length ratio: It can be seen that gut w
火龙果是一种以仙人掌为基础的水果,对健康有很多好处,比如降低血糖飙升的风险,帮助消化等。其营养成分富含维生素C、维生素E、镁、铁等。其生理调节功能是众所周知的。炎症性肠病(IBD)是多因素慢性肠道疾病。目前推荐美沙拉明等药物治疗IBD。然而,IBD的病因尚不清楚,这是一个持续的挑战,治疗药物的副作用也必须考虑在内。因此,本研究的目的是通过对2%葡聚糖硫酸钠(DSS)诱导的IBD小鼠模型给予三剂量火龙果粉,评估其对IBD症状的疗效和治疗策略的研究。将雌性C57BL/6小鼠分别分为正常对照组(n = 10)、阴性对照组(n = 10)和火龙果粉(250 mg/kg BW、500 mg/kg BW、1000 mg/kg BW) 3个剂量组(每组n = 10)。除正常对照组外,其余各组均给予2% DSS治疗,疗程5 d。随后给予C57BL/6小鼠正常饮水,直至实验结束。试验结束时,对大鼠体重(BW)、粪便外观/状态、宏观和微观结肠损伤、髓过氧化物酶(MPO)活性进行监测、测量和评分。结果表明,阴性对照组和3个火龙果粉组C57BL/6小鼠的体重在2% DSS诱导IBD期间逐渐降低,将饮用水中的2% DSS替换为正常饮用水后,C57BL/6小鼠的体重逐渐升高。试验进行到第3 ~ 4周时,阴性对照组的体重显著低于正常对照组。大便外观/状态显示,阴性对照组大便评分显著高于正常对照组(p <0.001)。火龙果散高剂量组大便评分显著低于阴性对照组(p <0.001)。实验结束时对C57BL/6小鼠结肠进行宏观观察。(1)肠道重量:可以看出,各组中正常对照组肠道重量最低,阴性对照组肠道重量最高。阴性对照组的肠道重量均高于正常对照组、中剂量龙果粉组和高剂量龙果粉组。(2)肠长:可以看出正常对照组肠长最长。正常对照组肠道长度明显长于其他各组。除正常对照组外,其余各组间差异无统计学意义。(3)肠道重量-肠道长度比:可以看出,正常对照组的肠道重量-肠道长度比最低,阴性对照组的肠道重量-肠道长度比最高。阴性对照组肠重肠长比显著高于正常对照组(p <0.01)。高剂量火龙果粉组大鼠肠道重量与肠道长度之比显著低于阴性对照组。实验结束时取C57BL/6小鼠显微结肠。病理分析项目分为溃疡面积比、黏膜溃疡深度、炎症细胞浸润、黏膜下水肿。2% dss诱导组的组织病理学总评分也显著高于正常对照组。最后用MPO活性测定试剂盒评价MPO活性。可见,阴性对照组的MPO活性明显高于正常对照组。3个龙果粉组的MPO活性均显著低于阴性对照组。综上所述,在2% dss诱导的IBD小鼠模型中,中剂量(500 mg/kg BW)和高剂量(1000 mg/kg BW)龙果粉对缓解IBD引起的各种症状有积极的改善作用。
{"title":"Dragon fruit (Hylocereus undatus (Haw.) Britt.) powders attenuate ulcerative disease symptoms via dextran sulfate sodium-induced mouse model","authors":"None Yu-Wen Hung, None Chia-Chi Chen, None Yun-Xuan Chang, None Tzu-Yun Chi, None Ya-Peng Wang, None Tsung-Han Wu, None Ya-Ling Cyue, None Pi-Hsin Chen, None Yen-Jung Lu, None Shih-Yi Guo, None Suz-Ching Ke, None Yu-Ying Fang, None Szu-Ping Sung, None Yan-Zhong Wu, None Chien-Chao Chiu, None Ching-Feng Chiu, None Hsuan-Wen Chiu, None Wei-Huang Tsai, None Yu-Hsing Lin, None Shao-Wen Hung","doi":"10.56781/ijsrls.2023.2.1.0030","DOIUrl":"https://doi.org/10.56781/ijsrls.2023.2.1.0030","url":null,"abstract":"Dragon fruit is cactus based fruit that has loads of health benefits such as lowering risks of a blood sugar spike, aids in digestion etc. Its nutritional content is rich in vitamin C, vitamin E, magnesium, iron etc. Its functions on the physiological regulation are well known. Inflammatory bowel diseases (IBD) are multifactorial chronic intestinal disorders. Currently, mesalamine etc. and therapeutic strategies were suggested for IBD therapy. However, the etiology of IBD remains unclear which is an ongoing challenge and side effects of therapeutic drugs must be also considered. Thus, the aim of this study was evaluated the efficacy and therapeutic strategies investigations on the attenuated IBD symptoms via administrating three doses of dragon fruit powders in the 2% dextran sulfate sodium (DSS)-induced IBD mouse model. The female C57BL/6 mice were divided respectively the normal control group (n = 10), the negative control group (n = 10), three dose groups (n = 10 per group) of dragon fruit powders (250 mg/kg BW, 500 mg/kg BW, and 1,000 mg/kg BW). Exception of the normal control group, other groups were administrated with 2% DSS for 5 days. Later, the normal drinking water was provide to C57BL/6 mice until the end of the experiment. At the end of the experiment, the body weight (BW), the stool appearance/status, the macroscopic and microscopic colonic injuries, and myeloperoxidase (MPO) activity were monitored, measured and scored. The results were showed that BW of C57BL/6 mice in the negative control group, three dragon fruit powder groups was gradually reduced during the IBD period induced by 2% DSS, and BW of C57BL/6 mice gradually increased when the 2% DSS in drinking water was replaced with the normal drinking water. When the experiment was carried out to the 3rd to 4th week, BW of the negative control group was significantly lower than that of the normal control group. The stool appearance/status was presented that stool score in the negative control group was significantly higher than that in the normal control group (p < 0.001). The stool score in the high-dose dragon fruit powder group was significantly lower than that in the negative control group (p < 0.001). The macroscopic colons of C57BL/6 mice were performed at the end of the experiment. (1) Gut weight: It can be seen that gut weight in the normal control group is lowest and the gut weight in the negative control group is highest between all groups. The gut weight in the negative control group was higher than that in the normal control group, medium-dose dragon fruit powder group, and high-dose dragon fruit powder group were seen. (2) Gut length: It can be seen that gut length in the normal control group is longest. The gut length in the normal control group is significantly longer than that in the other groups. Exception of the normal control group, other groups were not significant difference compared to each other. (3) Gut weight-to-gut length ratio: It can be seen that gut w","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136271509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic antibacterial effect of ethanolic extract of Carica papaya and Psidium guajava leave extract on Salmonella typhi, Escherichia coli and Klebsiella pneumoniae 番木瓜乙醇提取物和番石榴叶提取物对伤寒沙门菌、大肠杆菌和肺炎克雷伯菌的协同抑菌作用
Pub Date : 2022-09-30 DOI: 10.56781/ijsrls.2022.1.1.0025
Alexander Philip, Aisha E Ibrahim, Zakari Lois
This study assessed the synergistic antibacterial effect of ethanol extract of Carica papaya and Psidium guajava on Salmonella typhi, Escherichia coli and Klebsiella pneumoniae using agar well diffusion method and microdilution with Microtitre plates. Qualitative phytochemical analysis of the extract reveals the presence of alkaloids, saponins, glycosides, steroids, soluble carbohydrates and phenols. Five (5) grams of the freeze-dried extract was dissolved in 10ml of 10% Dimethyl Sulphoxide (DMSO) and used for sensitivity against selected standard isolates. Upon sensitivity testing, this study observes a significant inhibition zone diameter at the highest concentration of the extract (500 mg/ml) against Klebsiella pneumoniae with an inhibition zone diameter of 31.0mm relative to the inhibition zone diameters of Escherichia coli and Salmonella typhi with (19.5 mm) and (20.5 mm) respectively. The inhibition zone diameters of the positive control (ciprofloxacin solution) at 500 mg/mL for Klebsiella pneumoniae, Escherichia coli and Salmonella typhi was 36.0 mm, 37.5 mm and 34.5 mm respectively. Escherichia coli has minimal inhibitory concentration (MIC) of 0.977 mg/ml while Klebsiella pneumoniae and Salmonella typhi have their minimal inhibitory concentration (MIC) at 31.25 mg/ml and 62.5 mg/ml respectively.
本研究采用琼脂孔扩散法和微滴板微量稀释法,考察番木瓜和瓜爪哇番石榴醇提物对伤寒沙门氏菌、大肠杆菌和肺炎克雷伯菌的协同抑菌作用。提取物的定性植物化学分析显示存在生物碱、皂苷、糖苷、类固醇、可溶性碳水化合物和酚类。将5克冻干提取物溶解在10ml的10%二甲基亚砜(DMSO)中,对选定的标准分离株进行敏感性测试。经敏感性试验,本研究发现最高浓度(500 mg/ml)提取物对肺炎克雷伯菌的抑菌带直径显著,为31.0mm,而对大肠杆菌和伤寒沙门氏菌的抑菌带直径分别为19.5 mm和20.5 mm。阳性对照(环丙沙星溶液)500 mg/mL时对肺炎克雷伯菌、大肠杆菌和伤寒沙门菌的抑菌带直径分别为36.0 mm、37.5 mm和34.5 mm。大肠杆菌的最小抑制浓度(MIC)为0.977 mg/ml,肺炎克雷伯菌和伤寒沙门氏菌的最小抑制浓度(MIC)分别为31.25 mg/ml和62.5 mg/ml。
{"title":"Synergistic antibacterial effect of ethanolic extract of Carica papaya and Psidium guajava leave extract on Salmonella typhi, Escherichia coli and Klebsiella pneumoniae","authors":"Alexander Philip, Aisha E Ibrahim, Zakari Lois","doi":"10.56781/ijsrls.2022.1.1.0025","DOIUrl":"https://doi.org/10.56781/ijsrls.2022.1.1.0025","url":null,"abstract":"This study assessed the synergistic antibacterial effect of ethanol extract of Carica papaya and Psidium guajava on Salmonella typhi, Escherichia coli and Klebsiella pneumoniae using agar well diffusion method and microdilution with Microtitre plates. Qualitative phytochemical analysis of the extract reveals the presence of alkaloids, saponins, glycosides, steroids, soluble carbohydrates and phenols. Five (5) grams of the freeze-dried extract was dissolved in 10ml of 10% Dimethyl Sulphoxide (DMSO) and used for sensitivity against selected standard isolates. Upon sensitivity testing, this study observes a significant inhibition zone diameter at the highest concentration of the extract (500 mg/ml) against Klebsiella pneumoniae with an inhibition zone diameter of 31.0mm relative to the inhibition zone diameters of Escherichia coli and Salmonella typhi with (19.5 mm) and (20.5 mm) respectively. The inhibition zone diameters of the positive control (ciprofloxacin solution) at 500 mg/mL for Klebsiella pneumoniae, Escherichia coli and Salmonella typhi was 36.0 mm, 37.5 mm and 34.5 mm respectively. Escherichia coli has minimal inhibitory concentration (MIC) of 0.977 mg/ml while Klebsiella pneumoniae and Salmonella typhi have their minimal inhibitory concentration (MIC) at 31.25 mg/ml and 62.5 mg/ml respectively.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125430458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Different processing methods of pigeon pea (Cajanus cajan) seed meal: Effect on nutritive value, growth and production performance of commercial layers 不同加工方法对商品蛋鸡营养价值、生长和生产性能的影响
Pub Date : 2022-09-30 DOI: 10.56781/ijsrls.2022.1.1.0023
Aman Getiso, Etalem Tesfaye, Diribi Mijena
The experiment was conducted with 150 Bovans brown pullets to evaluate the performance of grower pullets and layers fed raw or processed pigeon pea seed meal diets from 18th week stage of life. Each treatment had three replicates and ten birds per replicate in a completely randomized design (CRD) with pullets and layers fed comprised of 20% pigeon pea seed meal diets that were isoenergetic and isonitrogenous. Live weight, feed intake, live weight gain, egg production and mortality parameters were measured. Pullets fed 20% boiled and soaked PPSM diets attained significantly higher (P<0.05) final body weight at point of lay and daily feed intake than those fed control, raw and toasted PPSM diets. Results revealed that non-significant (P>0.05) differences among the layers fed PPSM diets in hen-day egg production, average daily feed intake and average mortality parameters but significant differences (p<0.05) were observed on hen-housed egg production and age at first egg lay parameters. It was concluded that PPSM could be a good protein source for grower pullets and layers, which could be incorporated into the diets at 20% of the whole diet without any adverse effect on growth and egg production performance.
本试验以150只bovan褐蛋鸡为试验对象,从18周龄开始,分别饲喂原料或加工过的鸽豆籽粕饲粮,对生长仔鸡和蛋鸡的生产性能进行评价。采用完全随机设计(CRD),每个处理设3个重复,每个重复10只鸡,仔鸡和蛋鸡分别饲喂20%等能等氮鸽豌豆粕饲粮。测定活重、采食量、活增重、产蛋量和死亡率等参数。蛋鸡日产蛋量、平均日采食量和平均死亡率在饲粮中差异显著(p<0.05),但在鸡舍产蛋量和初产蛋龄参数上差异显著(p<0.05)。综上所述,PPSM可作为一种良好的蛋白质来源,在全饲粮中添加20%的PPSM对生长和产蛋性能无不良影响。
{"title":"Different processing methods of pigeon pea (Cajanus cajan) seed meal: Effect on nutritive value, growth and production performance of commercial layers","authors":"Aman Getiso, Etalem Tesfaye, Diribi Mijena","doi":"10.56781/ijsrls.2022.1.1.0023","DOIUrl":"https://doi.org/10.56781/ijsrls.2022.1.1.0023","url":null,"abstract":"The experiment was conducted with 150 Bovans brown pullets to evaluate the performance of grower pullets and layers fed raw or processed pigeon pea seed meal diets from 18th week stage of life. Each treatment had three replicates and ten birds per replicate in a completely randomized design (CRD) with pullets and layers fed comprised of 20% pigeon pea seed meal diets that were isoenergetic and isonitrogenous. Live weight, feed intake, live weight gain, egg production and mortality parameters were measured. Pullets fed 20% boiled and soaked PPSM diets attained significantly higher (P<0.05) final body weight at point of lay and daily feed intake than those fed control, raw and toasted PPSM diets. Results revealed that non-significant (P>0.05) differences among the layers fed PPSM diets in hen-day egg production, average daily feed intake and average mortality parameters but significant differences (p<0.05) were observed on hen-housed egg production and age at first egg lay parameters. It was concluded that PPSM could be a good protein source for grower pullets and layers, which could be incorporated into the diets at 20% of the whole diet without any adverse effect on growth and egg production performance.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132308364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of concurrent food-chain simulation of cadmium and crude petroleum oil pollutions on amylase activity in Wistar rats 镉和原油污染同步食物链模拟对Wistar大鼠淀粉酶活性的影响
Pub Date : 2022-09-30 DOI: 10.56781/ijsrls.2022.1.1.0024
Richard Omonigho Ovie, George Edaghogho Eriyamremu
The bioavailability levels of cadmium (Cd) and (CPO) in water and polluted food has become worrisome over the years. The study was aimed at investigating the effects of concurrent food-chain simulation of cadmium and crude petroleum oil pollution on amylase activity in Wistar rats. Simulation of both Cd and CPO pollution was done in the water in which catfish were housed for 1 month at a daily dose of 4 and 0.8 ppm respectively. The fish were then used as source of protein in formulation of diet for feeding rats for 1 and 2 months respectively. The rats were granted access to drinking water at will, weighed at the end of the feeding periods and sacrificed followed by removal of the duodenal portion of the small intestine. Weight gain and intestinal/body weight ratio was measured and the activity of amylase assayed. The results showed a non-significant difference (p ˃ 0.05) in the intestinal/body weight ratio and amylase activity after 1 month of simulated pollution. While, there was a significant decrease (p < 0.05) in weight gain and amylase activity after 2 months. Cd and CPO mixture therefore, has a deleterious effect on the environment and subsequently animals especially man as the final consumer in the food-chain.
近年来,镉(Cd)和(CPO)在水和污染食品中的生物利用度水平令人担忧。本研究旨在探讨镉和原油污染并发食物链模拟对Wistar大鼠淀粉酶活性的影响。在饲养鲶鱼1个月的水中分别以4 ppm和0.8 ppm的日剂量对Cd和CPO污染进行了模拟。然后将这些鱼作为蛋白质来源,分别饲喂大鼠1个月和2个月。这些大鼠可以随意喝水,在喂食期结束时称重,然后处死,然后切除小肠的十二指肠部分。测定增重和肠重比,测定淀粉酶活性。结果表明,模拟污染1个月后,肠道/体重比和淀粉酶活性差异无统计学意义(p > 0.05)。2个月后,增重和淀粉酶活性显著降低(p < 0.05)。因此,Cd和CPO混合物对环境和随后的动物,特别是作为食物链最终消费者的人类产生有害影响。
{"title":"Effects of concurrent food-chain simulation of cadmium and crude petroleum oil pollutions on amylase activity in Wistar rats","authors":"Richard Omonigho Ovie, George Edaghogho Eriyamremu","doi":"10.56781/ijsrls.2022.1.1.0024","DOIUrl":"https://doi.org/10.56781/ijsrls.2022.1.1.0024","url":null,"abstract":"The bioavailability levels of cadmium (Cd) and (CPO) in water and polluted food has become worrisome over the years. The study was aimed at investigating the effects of concurrent food-chain simulation of cadmium and crude petroleum oil pollution on amylase activity in Wistar rats. Simulation of both Cd and CPO pollution was done in the water in which catfish were housed for 1 month at a daily dose of 4 and 0.8 ppm respectively. The fish were then used as source of protein in formulation of diet for feeding rats for 1 and 2 months respectively. The rats were granted access to drinking water at will, weighed at the end of the feeding periods and sacrificed followed by removal of the duodenal portion of the small intestine. Weight gain and intestinal/body weight ratio was measured and the activity of amylase assayed. The results showed a non-significant difference (p ˃ 0.05) in the intestinal/body weight ratio and amylase activity after 1 month of simulated pollution. While, there was a significant decrease (p < 0.05) in weight gain and amylase activity after 2 months. Cd and CPO mixture therefore, has a deleterious effect on the environment and subsequently animals especially man as the final consumer in the food-chain.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123066133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Demonstration and participatory evaluation of apiculture technologies in Sidama national regional state, Ethiopia 埃塞俄比亚西达马民族地区州养蜂技术的示范和参与性评价
Pub Date : 2022-09-30 DOI: 10.56781/ijsrls.2022.1.1.0021
Dinku Negash, Teklu Gebretsadik, Amare Argaw
Demonstration and Participatory evaluations of improved beekeeping technologies were conducted in Loka Abaya and Hawassa Zuriya woredas of Sidama National Regional state with the objective of demonstrating improved beekeeping technologies & strengthen research extension farmer’s linkage in beekeeping technology generation and transfer. Demonstration and evaluations of frame and transitional hive was conducted by forming farmers research groups (FRGs) at 4 peasant association (PAs) of selected district; from the demonstration an average of 25.5kg semi-extracted and 10.5 kg crude honey per hive/annum was harvested using after and before using improved technologies respectively and bees wax purification was also demonstrated on farmers field. Partial budget analyses implied that adoption of improved beekeeping technologies make small holder beekeepers more profitable than traditional practice. Therefore, all the demonstrated and evaluated technologies have been recommended for the mandate area to further promote the technologies in to the areas where there is a gap in utilizing and disseminating the technologies.
在西达马民族地区州的Loka Abaya和Hawassa Zuriya工厂进行了改良养蜂技术的示范和参与性评估,目的是展示改良养蜂技术并加强研究推广,农民在养蜂技术产生和转让方面的联系。在选定地区的4个农协组成农民研究组,对框架和过渡蜂箱进行论证和评价;从示范中,每箱每年平均收获25.5公斤半采蜜和10.5公斤粗采蜜,分别采用改进技术后和改进技术前,并在农民田间演示了蜂蜡净化。部分预算分析表明,采用改进的养蜂技术使小型养蜂人比传统做法更有利可图。因此,已为任务领域推荐了所有已展示和评价的技术,以便在利用和传播这些技术方面存在差距的领域进一步推广这些技术。
{"title":"Demonstration and participatory evaluation of apiculture technologies in Sidama national regional state, Ethiopia","authors":"Dinku Negash, Teklu Gebretsadik, Amare Argaw","doi":"10.56781/ijsrls.2022.1.1.0021","DOIUrl":"https://doi.org/10.56781/ijsrls.2022.1.1.0021","url":null,"abstract":"Demonstration and Participatory evaluations of improved beekeeping technologies were conducted in Loka Abaya and Hawassa Zuriya woredas of Sidama National Regional state with the objective of demonstrating improved beekeeping technologies & strengthen research extension farmer’s linkage in beekeeping technology generation and transfer. Demonstration and evaluations of frame and transitional hive was conducted by forming farmers research groups (FRGs) at 4 peasant association (PAs) of selected district; from the demonstration an average of 25.5kg semi-extracted and 10.5 kg crude honey per hive/annum was harvested using after and before using improved technologies respectively and bees wax purification was also demonstrated on farmers field. Partial budget analyses implied that adoption of improved beekeeping technologies make small holder beekeepers more profitable than traditional practice. Therefore, all the demonstrated and evaluated technologies have been recommended for the mandate area to further promote the technologies in to the areas where there is a gap in utilizing and disseminating the technologies.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131548439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Crop water requirement and irrigation scheduling of dry season irrigated crops in Pawe district, lowland hot humid area of Ethiopia 埃塞俄比亚低地湿热地区Pawe地区旱季灌溉作物需水量及灌溉调度
Pub Date : 2022-09-30 DOI: 10.56781/ijsrls.2022.1.1.0022
Temesgen F. Adamtie, Abeba H. selie, Demeke T. Mitku
Knowledge of irrigation water requirements and irrigation time schedules improves irrigation water management and crop productivity in the field. The CROPWAT model calculates the ETo, CWR, and IRs to develop the irrigation schedules under different administration conditions and water supply plans. The objective of this study was to determine the crop water requirement of tomato, onion and soybean crops and their irrigation scheduling under local climate condition of Pawe district. Local climate, crop type and soil data were used as input data to determine crop water requirement (CWR) and irrigation scheduling. The finding of this study was crop water requirements of tomato, onion and soyabean were 547.7, 362.8 and 337 mm respectively per season on black clay soil of Pawe district. The obtained irrigation scheduling were twelve irrigation schedules for tomatoes, elven for onion and five for soybean. The study will help to improve the management of water resources and loss of crop productivity. This study can be used as a reference for decision-making for future irrigation scheme planning and irrigation water development of Pawe district.
了解灌溉用水需求和灌溉时间安排可以改善农田灌溉用水管理和作物生产力。CROPWAT模型通过计算ETo、CWR和ir来制定不同管理条件和供水计划下的灌溉计划。本研究旨在确定坡威县当地气候条件下番茄、洋葱和大豆作物需水量及其灌溉调度。利用当地气候、作物类型和土壤数据作为输入数据,确定作物需水量(CWR)和灌溉调度。结果表明:在包洼地区黑土上,番茄、洋葱和大豆的季需水量分别为547.7、362.8和337 mm。得到的灌溉调度为番茄12个,洋葱11个,大豆5个。这项研究将有助于改善水资源和作物生产力损失的管理。研究结果可为今后坡威区灌溉方案规划和灌溉水开发提供决策参考。
{"title":"Crop water requirement and irrigation scheduling of dry season irrigated crops in Pawe district, lowland hot humid area of Ethiopia","authors":"Temesgen F. Adamtie, Abeba H. selie, Demeke T. Mitku","doi":"10.56781/ijsrls.2022.1.1.0022","DOIUrl":"https://doi.org/10.56781/ijsrls.2022.1.1.0022","url":null,"abstract":"Knowledge of irrigation water requirements and irrigation time schedules improves irrigation water management and crop productivity in the field. The CROPWAT model calculates the ETo, CWR, and IRs to develop the irrigation schedules under different administration conditions and water supply plans. The objective of this study was to determine the crop water requirement of tomato, onion and soybean crops and their irrigation scheduling under local climate condition of Pawe district. Local climate, crop type and soil data were used as input data to determine crop water requirement (CWR) and irrigation scheduling. The finding of this study was crop water requirements of tomato, onion and soyabean were 547.7, 362.8 and 337 mm respectively per season on black clay soil of Pawe district. The obtained irrigation scheduling were twelve irrigation schedules for tomatoes, elven for onion and five for soybean. The study will help to improve the management of water resources and loss of crop productivity. This study can be used as a reference for decision-making for future irrigation scheme planning and irrigation water development of Pawe district.","PeriodicalId":191608,"journal":{"name":"International Journal of Scholarly Research in Life Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132181846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Scholarly Research in Life Sciences
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1