Kateřina Pražáková, Vladimír Vrkoslav, Štěpán Strnad, B. Muffová, S. Kauerova, Jiří Froněk, I. Králová Lesná, Rudolf Poledne, David Sýkora
Currently, an increasing incidence of cardiovascular diseases is observed in the population. Factors contributing to their development are obesity and pro-inflammatory processes occurring in the adipose tissue. Macrophages, cells of the non-specific immune system, which can be divided into pro-inflammatory and anti-inflammatory, play a significant role in these processes. For a better understanding of these phenomena, it is necessary to analyze the lipidome of macrophages of the two mentioned groups. The presented work was focused on the optimization of selected parameters of the LC-MS method for the analysis of 71 lipids from 16 lipid classes. Best results were achieved with column Acquity UPLC BEH C18 (1.7 µm, 2.1 mm × 100 mm) operating at the flow rate of 300 μL min–1 and temperature of 55 °C. The optimal desolvation temperature was 350 °C and the gradient elution started at 0 % of solvent B in the mobile phase. Retention dependencies were characterized for individual lipid classes. They were successfully fitted with a linear model (R2 > 0.99) and subsequently used to confirm the accuracy of lipid identification. The optimized LC-MS method was also used to analyze the real samples of macrophages isolated from the adipose tissue of kidney donors. Samples were provided by Institute for Clinical and Experimental Medicine. The principal component analysis gave insight into the division of macrophages according to their type. The volcano plot showed a significant increase in the concentration of five lipids in the case of pro-inflammatory macrophages.
{"title":"LC-MS Lipidomic Analysis of Macrophages","authors":"Kateřina Pražáková, Vladimír Vrkoslav, Štěpán Strnad, B. Muffová, S. Kauerova, Jiří Froněk, I. Králová Lesná, Rudolf Poledne, David Sýkora","doi":"10.54779/ccsss20240066","DOIUrl":"https://doi.org/10.54779/ccsss20240066","url":null,"abstract":"Currently, an increasing incidence of cardiovascular diseases is observed in the population. Factors contributing to their development are obesity and pro-inflammatory processes occurring in the adipose tissue. Macrophages, cells of the non-specific immune system, which can be divided into pro-inflammatory and anti-inflammatory, play a significant role in these processes. For a better understanding of these phenomena, it is necessary to analyze the lipidome of macrophages of the two mentioned groups. The presented work was focused on the optimization of selected parameters of the LC-MS method for the analysis of 71 lipids from 16 lipid classes. Best results were achieved with column Acquity UPLC BEH C18 (1.7 µm, 2.1 mm × 100 mm) operating at the flow rate of 300 μL min–1 and temperature of 55 °C. The optimal desolvation temperature was 350 °C and the gradient elution started at 0 % of solvent B in the mobile phase. Retention dependencies were characterized for individual lipid classes. They were successfully fitted with a linear model (R2 > 0.99) and subsequently used to confirm the accuracy of lipid identification. The optimized LC-MS method was also used to analyze the real samples of macrophages isolated from the adipose tissue of kidney donors. Samples were provided by Institute for Clinical and Experimental Medicine. The principal component analysis gave insight into the division of macrophages according to their type. The volcano plot showed a significant increase in the concentration of five lipids in the case of pro-inflammatory macrophages.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"54 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140983568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Solid bismuth drop electrode (SBiDE) is a new working electrode commercially available on the Czech market from 2020 by the company Metrohm. The aim of this work was to verify the applicability of the SBiDE in the voltammetric determination of a model organic substance. According to the available information, this is the very first published work on this topic. Metronidazole (an antibiotic used to treat diseases caused by both Gram-positive and Gram-negative anaerobic bacteria) was chosen in this study as a model drug representing electrochemically reducible biologically active compounds. Under optimum conditions (Britton-Robinson buffer of pH 12.0 used as the supporting electrolyte and no electrochemical regeneration of the working electrode surface), a linear calibration dependence of metronidazole was recorded in the concentration range from 1 to 600 μmol L–1, with the limits of detection (LOD) and quantification (LOQ) of 0.41 μmol L–1 and 1.4 μmol L–1, respectively. The newly developed differential pulse voltammetric (DPV) method has also been successfully applied in the determination of metronidazole in various dosage forms.
{"title":"Solid Bismuth Drop Electrode – A New Tool for Voltammetric Determination of Electrochemically Reducible Organic Compounds","authors":"Johanka Jarošová, V. Vyskočil","doi":"10.54779/ccsss20240061","DOIUrl":"https://doi.org/10.54779/ccsss20240061","url":null,"abstract":"Solid bismuth drop electrode (SBiDE) is a new working electrode commercially available on the Czech market from 2020 by the company Metrohm. The aim of this work was to verify the applicability of the SBiDE in the voltammetric determination of a model organic substance. According to the available information, this is the very first published work on this topic. Metronidazole (an antibiotic used to treat diseases caused by both Gram-positive and Gram-negative anaerobic bacteria) was chosen in this study as a model drug representing electrochemically reducible biologically active compounds. Under optimum conditions (Britton-Robinson buffer of pH 12.0 used as the supporting electrolyte and no electrochemical regeneration of the working electrode surface), a linear calibration dependence of metronidazole was recorded in the concentration range from 1 to 600 μmol L–1, with the limits of detection (LOD) and quantification (LOQ) of 0.41 μmol L–1 and 1.4 μmol L–1, respectively. The newly developed differential pulse voltammetric (DPV) method has also been successfully applied in the determination of metronidazole in various dosage forms.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140983972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jakub Harvalík, Kateřina Hrubešová, Ondřej Vrtělka, V. Setnička
This work focuses on the preparation of colloidal solutions of Au and Ag nanoparticles and verification of their applicability for surface-enhanced Raman scattering spectroscopy of blood plasma. The composition of blood plasma may change due to pathobiochemical processes associated with disease development. Analysis of blood plasma might reveal these changes and thus help in the diagnostics process of various diseases. Raman spectroscopy appears to be an ideal method for rapid and non-destructive analysis of blood plasma. Moreover, surface-enhanced Raman scattering spectroscopy might allow the detection of very low levels of potential disease-specific markers. In this work, the prepared colloidal solutions of Au and Ag nanoparticles were first characterized by UV‑VIS absorption spectroscopy and their enhancing capabilities were verified using riboflavin as a model analyte. Subsequently, optimization of the measurement conditions for surface-enhanced Raman spectroscopy of blood plasma was performed. The results show that at the excitation wavelength of 785 nm, significantly higher enhancement is achieved when using Ag nanoparticles. In the case of Au nanoparticles, the enhanced blood plasma spectrum was successfully detected only once, and the addition of an aggregating agent (NaCl) was required.
{"title":"Study of the Applicability of Colloidal Solutions of Gold and Silver Nanoparticles for Surface-enhanced Raman Spectroscopy of Blood Plasma","authors":"Jakub Harvalík, Kateřina Hrubešová, Ondřej Vrtělka, V. Setnička","doi":"10.54779/ccsss20220327","DOIUrl":"https://doi.org/10.54779/ccsss20220327","url":null,"abstract":"This work focuses on the preparation of colloidal solutions of Au and Ag nanoparticles and verification of their applicability for surface-enhanced Raman scattering spectroscopy of blood plasma. The composition of blood plasma may change due to pathobiochemical processes associated with disease development. Analysis of blood plasma might reveal these changes and thus help in the diagnostics process of various diseases. Raman spectroscopy appears to be an ideal method for rapid and non-destructive analysis of blood plasma. Moreover, surface-enhanced Raman scattering spectroscopy might allow the detection of very low levels of potential disease-specific markers. In this work, the prepared colloidal solutions of Au and Ag nanoparticles were first characterized by UV‑VIS absorption spectroscopy and their enhancing capabilities were verified using riboflavin as a model analyte. Subsequently, optimization of the measurement conditions for surface-enhanced Raman spectroscopy of blood plasma was performed. The results show that at the excitation wavelength of 785 nm, significantly higher enhancement is achieved when using Ag nanoparticles. In the case of Au nanoparticles, the enhanced blood plasma spectrum was successfully detected only once, and the addition of an aggregating agent (NaCl) was required.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127641621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid and sensitive detection of clinically important analytes is crucial for early disease diagnosis and initiation of treatment. Immunochemical assays are widely used to determine the presence and concentration of analytes in various complex media due to their high specificity provided by antibodies. Recently, lateral flow immunoassays (LFIAs) are becoming one of the most popular forms of immunochemical assays. LFIAs are test strips based on nitrocellulose membranes, and their most significant advantage is that there is no need for laboratory equipment to perform the assay enabling their use for point-of-care testing. Most LFIAs are based on gold nanoparticles; however, they often limit the resulting sensitivity of LFIAs. Our work focused on photon-upconversion nanoparticles (UCNPs) as a sensitive alternative label. UCNPs are lanthanide-based nanocrystals exhibiting anti-Stokes luminescence (excitation by the NIR laser and detection in the Vis region), which allows detection without optical background interference. We have developed an LFIA assay for human serum albumin (HSA) with UCNPs as a label. Using a specific anti-HSA antibody and a control anti-mouse antibody, various LFIA parameters were optimized. The immobilization of antibodies was done using acetate buffer with 1% methanol, glass membrane ST17 was used as a conjugate pad, and Tris buffer with 5% sucrose was used to stabilize the particles within the conjugate pad. The choice of a different nitrocellulose membrane had a relatively small effect on assay performance; the best results were achieved with membrane 120 HP Plus Thick. Our work has successfully demonstrated the potential of using UCNPs as sensitive labels in LFIA tests.
快速、灵敏地检测临床重要分析物对于疾病的早期诊断和开始治疗至关重要。由于抗体提供的高特异性，免疫化学分析被广泛用于确定各种复杂介质中分析物的存在和浓度。近年来，侧流免疫分析(LFIAs)已成为最流行的免疫化学分析方法之一。lfia是基于硝化纤维素膜的试纸条，其最大的优点是不需要实验室设备来进行分析，使其能够用于即时检测。大多数LFIAs是基于金纳米粒子的;然而，它们往往限制了LFIAs的灵敏度。我们的工作重点是光子上转换纳米粒子(UCNPs)作为一种敏感的替代标签。UCNPs是一种基于镧系元素的纳米晶体，具有反斯托克斯发光(由近红外激光激发并在可见光区检测)，可以在没有光学背景干扰的情况下进行检测。我们开发了一种以UCNPs为标签的人血清白蛋白(HSA) LFIA检测方法。采用特异性抗hsa抗体和对照抗小鼠抗体，对LFIA各项参数进行优化。抗体固定采用1%甲醇醋酸缓冲液，ST17玻璃膜作为偶联垫，5%蔗糖Tris缓冲液稳定偶联垫内的颗粒。选择不同的硝化纤维素膜对测定性能的影响相对较小;120 HP Plus厚膜的效果最好。我们的工作已经成功地证明了在LFIA测试中使用UCNPs作为敏感标签的潜力。
{"title":"Detection of Protein Biomarkers Using Lateral Flow Tests with Photon-upconversion Nanoparticles","authors":"Eliška Macháčová, Z. Farka","doi":"10.54779/ccsss20220335","DOIUrl":"https://doi.org/10.54779/ccsss20220335","url":null,"abstract":"Rapid and sensitive detection of clinically important analytes is crucial for early disease diagnosis and initiation of treatment. Immunochemical assays are widely used to determine the presence and concentration of analytes in various complex media due to their high specificity provided by antibodies. Recently, lateral flow immunoassays (LFIAs) are becoming one of the most popular forms of immunochemical assays. LFIAs are test strips based on nitrocellulose membranes, and their most significant advantage is that there is no need for laboratory equipment to perform the assay enabling their use for point-of-care testing. Most LFIAs are based on gold nanoparticles; however, they often limit the resulting sensitivity of LFIAs. Our work focused on photon-upconversion nanoparticles (UCNPs) as a sensitive alternative label. UCNPs are lanthanide-based nanocrystals exhibiting anti-Stokes luminescence (excitation by the NIR laser and detection in the Vis region), which allows detection without optical background interference. We have developed an LFIA assay for human serum albumin (HSA) with UCNPs as a label. Using a specific anti-HSA antibody and a control anti-mouse antibody, various LFIA parameters were optimized. The immobilization of antibodies was done using acetate buffer with 1% methanol, glass membrane ST17 was used as a conjugate pad, and Tris buffer with 5% sucrose was used to stabilize the particles within the conjugate pad. The choice of a different nitrocellulose membrane had a relatively small effect on assay performance; the best results were achieved with membrane 120 HP Plus Thick. Our work has successfully demonstrated the potential of using UCNPs as sensitive labels in LFIA tests.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134185498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adam Březina, Adéla Dořmanová, P. Kukučka, P. Lubal, M. Farková
This paper is focused on study of the concentration of the basic catalyst in methanol on the yield of the derivatization transesterification reaction of triacyglycerols. Potassium hydroxide and sodium hydroxide solutions were used as basic catalysts. Triacylglycerols were transesterified, rapeseed oil was used as a sample. Triacylglycerols were converted to methyl esters of fatty acids, which were subsequently determined by GC-MS where the peak areas were monitored, because they represent the yield of the formed fatty acid methyl esters, namely palmitic, oleic, linoleic and stearic acids. The highest yield of transesterification of the oil to methylesters of fatty acids was found for a concentration of 3.0 mol dm–3 for KOH and 2.0 mol dm–3 for NaOH. At higher catalyst concentrations, a predominant side-reaction, saponification, was observed, which decreased the overall yield of fatty acid methyl esters.
{"title":"Application of Transesterification Derivatization Reaction for GC-MS Analysis","authors":"Adam Březina, Adéla Dořmanová, P. Kukučka, P. Lubal, M. Farková","doi":"10.54779/ccsss20220319","DOIUrl":"https://doi.org/10.54779/ccsss20220319","url":null,"abstract":"This paper is focused on study of the concentration of the basic catalyst in methanol on the yield of the derivatization transesterification reaction of triacyglycerols. Potassium hydroxide and sodium hydroxide solutions were used as basic catalysts. Triacylglycerols were transesterified, rapeseed oil was used as a sample. Triacylglycerols were converted to methyl esters of fatty acids, which were subsequently determined by GC-MS where the peak areas were monitored, because they represent the yield of the formed fatty acid methyl esters, namely palmitic, oleic, linoleic and stearic acids. The highest yield of transesterification of the oil to methylesters of fatty acids was found for a concentration of 3.0 mol dm–3 for KOH and 2.0 mol dm–3 for NaOH. At higher catalyst concentrations, a predominant side-reaction, saponification, was observed, which decreased the overall yield of fatty acid methyl esters.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115091958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The work dealt with the choice of a suitable solvent for extraction of ᴅ-panthenol from body milk samples and the choice of SPE adsorbent for its purification and preconcentration. The suitable extraction and HPLC conditions were applied for analysis of body milks containing ᴅ‑panthenol.
{"title":"The Choice of Solvent for the Extraction of ᴅ-panthenol from Samples of Body Milks","authors":"B. Simkova, K. Hroboňová, Andrea Špačková","doi":"10.54779/ccsss20220340","DOIUrl":"https://doi.org/10.54779/ccsss20220340","url":null,"abstract":"The work dealt with the choice of a suitable solvent for extraction of ᴅ-panthenol from body milk samples and the choice of SPE adsorbent for its purification and preconcentration. The suitable extraction and HPLC conditions were applied for analysis of body milks containing ᴅ‑panthenol.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114626024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study was to select suitable experimental conditions for extraction of coumarin by Ultrasonic Assisted Extraction (UAE). Type of extraction solvent, extraction time and temperature were optimized to increase the extraction yield of coumarin from samples of cinnamon and foods containing cinnamon. Optimal UAE conditions include extraction time of 10 min, temperature of 50 °C and extraction solvent based on choline chloride and ʟ-lactic acid. The extraction yields were comparable to traditional solvents – water and methanol.
{"title":"The Application of Ultrasonic Assisted Extraction in Analysis Cinnamon and Food Products","authors":"Kristína Čišovská, K. Hroboňová","doi":"10.54779/ccsss20220323","DOIUrl":"https://doi.org/10.54779/ccsss20220323","url":null,"abstract":"The objective of this study was to select suitable experimental conditions for extraction of coumarin by Ultrasonic Assisted Extraction (UAE). Type of extraction solvent, extraction time and temperature were optimized to increase the extraction yield of coumarin from samples of cinnamon and foods containing cinnamon. Optimal UAE conditions include extraction time of 10 min, temperature of 50 °C and extraction solvent based on choline chloride and ʟ-lactic acid. The extraction yields were comparable to traditional solvents – water and methanol.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117050202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adéla Koryťáková, Oleksandr Volochanskyi, M. Král, Marie Švecová
SERS spectroscopy remains not a well-established analytical technique mainly because of the complicated preparation of SERS-active substrates, which often have low reproducibility. For this reason, the development of new or optimization of existing substrates is important. For testing the SERS activity of these substrates, the so-called model analytes are commonly used. One of them is riboflavin, which is utilized for its non-toxicity and suitable optical properties. This work is focused on optimizing the enhancing properties of galvanically prepared silver substrates. The influence of the base material (Al and its alloys, Zn) and of the addition of a complexing agent (EDTA) into the Ag deposition bath on the spectral response of riboflavin was evaluated. Each prepared substrate was characterized by scanning electron microscopy, and the SERS spectra were collected using an excitation wavelength of 1064 nm. All data were subsequently processed and interpreted using multivariate statistics, namely principal component analysis. The most universal and suitable base material for the preparation of Ag substrate for riboflavin detection was the AlMg alloy. The addition of a complexing agent to the deposition bath changed the morphology of the prepared substrates and the highest spectral response of riboflavin was obtained by adding 0.55 mM EDTA. The intensity of riboflavin spectral response was comparable to the value obtained using substrates prepared in the Ag bath, but the substrates prepared in the AgNO3 solution achieved a weaker enhancement of the spectral response.
SERS光谱仍然不是一种完善的分析技术，主要是因为SERS活性底物的制备复杂，通常具有低重复性。因此，开发新的或优化现有基板是很重要的。为了测试这些底物的SERS活性，通常使用所谓的模型分析物。其中一种是核黄素，它具有无毒和合适的光学性质。本工作的重点是优化电法制备银衬底的增强性能。考察了基材(Al及其合金Zn)和络合剂(EDTA)对核黄素光谱响应的影响。利用扫描电镜对所制备的衬底进行了表征，并在1064 nm激发波长下采集了SERS光谱。所有数据随后处理和解释使用多元统计，即主成分分析。制备核黄素检测用银底物最普遍、最合适的基材是AlMg合金。在沉积液中加入络合剂改变了基底的形貌，加入0.55 mM EDTA时核黄素的光谱响应最高。核黄素的光谱响应强度与在Ag液中制备的底物的光谱响应强度相当，但在AgNO3溶液中制备的底物的光谱响应增强较弱。
{"title":"Influence of Base Material and Addition of Complexing Agent to Deposition Bath for Galvanic Preparation of SERS-active Layers and Spectra of Riboflavin","authors":"Adéla Koryťáková, Oleksandr Volochanskyi, M. Král, Marie Švecová","doi":"10.54779/ccsss20220331","DOIUrl":"https://doi.org/10.54779/ccsss20220331","url":null,"abstract":"SERS spectroscopy remains not a well-established analytical technique mainly because of the complicated preparation of SERS-active substrates, which often have low reproducibility. For this reason, the development of new or optimization of existing substrates is important. For testing the SERS activity of these substrates, the so-called model analytes are commonly used. One of them is riboflavin, which is utilized for its non-toxicity and suitable optical properties. \u0000This work is focused on optimizing the enhancing properties of galvanically prepared silver substrates. The influence of the base material (Al and its alloys, Zn) and of the addition of a complexing agent (EDTA) into the Ag deposition bath on the spectral response of riboflavin was evaluated. Each prepared substrate was characterized by scanning electron microscopy, and the SERS spectra were collected using an excitation wavelength of 1064 nm. All data were subsequently processed and interpreted using multivariate statistics, namely principal component analysis. The most universal and suitable base material for the preparation of Ag substrate for riboflavin detection was the AlMg alloy. The addition of a complexing agent to the deposition bath changed the morphology of the prepared substrates and the highest spectral response of riboflavin was obtained by adding 0.55 mM EDTA. The intensity of riboflavin spectral response was comparable to the value obtained using substrates prepared in the Ag bath, but the substrates prepared in the AgNO3 solution achieved a weaker enhancement of the spectral response.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"212 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133462186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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