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Field Assessment of an Immune-Complex Infectious Bursal Disease Vaccine in Chicks Born to Non-Hyperimmunized Broiler Breeders 一种免疫复合物感染性法氏囊病疫苗在未高度免疫的肉鸡种鸡所生雏鸡中的现场评价
Pub Date : 2018-08-01 DOI: 10.15744/2348-9790.6.302
C. Cazaban, Rietema Rmw, Wit Jjd, Palya, Y. Gardin
Infectious bursal disease (IBD) is a viral disease that affects chickens worldwide and it has a major economic impact on the modern poultry industry. It is caused by a birnavirus, called infectious bursal disease virus (IBDV), which is a bi-segmented, double stranded RNA virus, highly resistant in the environment. The RNA encodes for five viral proteins (VP1 to VP5) of which VP2 contains hypervariable portions that enable classifying strains into various antigenic and genetic groups; in addition, VP2 contains the majority of neutralizing sites. Depending on the pathogenic type, IBDV strains are classified as very virulent, virulent, and subclinical. Antigenically two serotypes (serotype 1 and 2) of IBDV exist, although antigenic variants are described within serotype 1 strains. Only the serotype 1 virus is pathogenic for chickens in which it can cause depression, diarrhoea, haemorrhages in muscles and proventriculus, and inflammation, necrosis and atrophy of the bursa of Fabricius depending on the virulence of the strain. Variant IBDV strains generally do not cause clinical signs or mortality, but can cause severe atrophy of the bursa without marked Prevention of infectious bursal disease (IBD) remains of paramount importance, given the dramatic losses it can induce in commercial chicken flocks. In the Netherlands, maternal immunity is regarded as low, because the parent stock flocks do not receive an inactivated vaccination prior to the onset of production. Therefore, the aim of this field study was to assess the consequences of hatchery vaccination against IBD using an immune-complex vaccine in broiler chicks that were provided with a limited amount of passive immunity. Three grow out cycles in two houses each (six flocks) were monitored after vaccination. The recorded parameters included (i) body and bursa weight, (ii) IBD antibody response, and (iii) histopathology and PCR from the bursa of Fabricius. Average growth was similar in all groups, although inferior to the breed’s standards. Average bursa weight faced some reduction as part of vaccine effect. Histopathology analysis of the bursa showed a transient inflammatory process, while PCR detected the vaccine strain only. Lastly, serology tests confirmed the successful immunization of all broiler flocks. The two commercial ELISA kits (Idexx and BioChek) failed to detect antibodies for a limited period of time, whereas virus neutralization (VN) test could detect antibodies for the entire broilers’ lifespan. Idexx and BioChek ELISA kits had the highest correlation coefficient (0.93), whereas the lowest correlation was found between Idexx ELISA and VN (0.68). BioChek ELISA and VN showed a correlation of 0.78. This is the first detailed investigation of immune-complex vaccine’s mechanism of action in field conditions in chicks with low maternal immunity at hatch (<4000) and using a comprehensive set of monitoring parameters. It also provides updated information about IBD serology tests.
传染性法氏囊病(IBD)是一种影响全世界鸡群的病毒性疾病,它对现代家禽业具有重大的经济影响。它是由一种被称为传染性法氏囊病病毒(IBDV)的病毒引起的,这是一种双节段、双链RNA病毒,在环境中具有高度抗性。该RNA编码五种病毒蛋白(VP1至VP5),其中VP2含有高可变部分,可将菌株分类为各种抗原和遗传群;此外,VP2含有大多数中和位点。根据致病类型,IBDV毒株可分为非常毒力型、毒力型和亚临床型。IBDV存在两种血清型(血清型1和血清型2)的抗原性,尽管在血清型1株中描述了抗原变异。只有血清1型病毒对鸡具有致病性,根据菌株的毒力不同,它可引起抑郁、腹泻、肌肉和前脑室出血、炎症、法氏囊坏死和萎缩。变异的IBDV毒株通常不会引起临床症状或死亡,但可引起法氏囊严重萎缩而没有明显的症状,考虑到它在商业鸡群中可能引起的巨大损失,预防传染性法氏囊病(IBD)仍然是至关重要的。在荷兰,产妇免疫力被认为很低,因为母畜群在开始生产之前没有接种灭活疫苗。因此,本实地研究的目的是评估在提供有限被动免疫的肉鸡中使用免疫复合物疫苗孵化场接种IBD的后果。接种疫苗后,分别在两个房屋(6个鸡群)中监测三个生长周期。记录的参数包括(i)体和法氏囊重量,(ii) IBD抗体反应,(iii)法氏囊的组织病理学和PCR。所有组的平均生长相似,尽管低于该品种的标准。由于疫苗的作用,平均法氏囊重量有所减少。法氏囊的组织病理学分析显示短暂的炎症过程,而PCR仅检测到疫苗株。最后,血清学试验证实了所有肉鸡群的成功免疫。两种商用ELISA试剂盒(Idexx和BioChek)在有限的时间内不能检测到抗体,而病毒中和(VN)试验可以在整个肉仔鸡的生命周期内检测到抗体。Idexx与BioChek的相关系数最高(0.93),而Idexx与VN的相关系数最低(0.68)。BioChek ELISA与VN的相关性为0.78。这是首次使用一套全面的监测参数,在野外条件下对孵化时母体免疫力较低(<4000)的雏鸡进行免疫复合物疫苗作用机制的详细调查。它还提供有关IBD血清学检测的最新信息。
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引用次数: 3
Prevalence of Mastitis and Associated risk factors in Jimma Town Dairy Farms, Western Ethiopia 埃塞俄比亚西部吉马镇奶牛场乳腺炎患病率及相关危险因素
Pub Date : 2018-08-01 DOI: 10.15744/2348-9790.6.304
Bayu Tesfaye, A. Abera
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引用次数: 4
Efficacy of Purified Glutathione -S- Transferee in Providing Protection against Haemonchus Contortus Infection in Sheep 纯化谷胱甘肽- s -转移体对绵羊弯曲血蜱感染的保护作用
Pub Date : 2018-08-01 DOI: 10.15744/2348-9790.6.305
Kandil Om, Salama Db, ELmetenawy Tm, Mousa Wm, Abo–Dahab Sm
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引用次数: 0
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Journal of Veterinary Science and Animal Husbandry
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