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Hemp seed priming via different agents to alleviate temperature stress 通过不同的药剂为大麻种子打底,以缓解温度胁迫
Pub Date : 2024-01-25 DOI: 10.15832/ankutbd.1391194
Sibel Day, Nilüfer Koçak, B. Önol
Soil temperature is an important factor in seed germination especially low soil temperature causes retarded and unsynchronized germination, emergence and seedling growth. These issues are causing the seedlings vulnerable to diseases and weed infestation during stand establishment of hemp. This study includes different seed priming agents (water, -0.8 MPa PEG 6000, 50 mMol thiamine and 10 mMol mannitol). These priming agents were controlled under different temperatures (10, 15, 20, 25 and 30 °C) to evaluate their potential for promoting germination and seedling growth. Results revealed that seed treatment gave accelerated mean germination time, increase emergence percentage at lower temperatures. Seed pre-treatments also promoted shoot length in all temperatures. However root length promoting effects of seed pre-treatments were more evident at 10, 15 and 20 °C. Seedling fresh and dry weight reached maximum values at 20 °C with water, thiamine and mannitol treatments. Chlorophyll and leaf proline content reached their higher values at 20, 25 and 30 °C. Overcoming temperature stress and promoting germination is important for hemp development. It was concluded that hemp seeds primed with water, thiamine, and mannitol had the highest biomass values for low, optimum, and high temperatures in this paper that makes these seed treatments suitable for hemp plants that could come across low or high temperatures during germination and early growth stages.
土壤温度是影响种子发芽的重要因素,尤其是低土壤温度会导致发芽、出苗和幼苗生长迟缓和不同步。这些问题都会导致大麻幼苗在生长过程中容易受到病害和杂草的侵扰。本研究采用了不同的催芽剂(水、-0.8 兆帕 PEG 6000、50 毫摩尔硫胺素和 10 毫摩尔甘露醇)。在不同温度(10、15、20、25 和 30 °C)下对这些催芽剂进行控制,以评估其促进发芽和幼苗生长的潜力。结果表明,在较低温度下,种子处理可加快平均发芽时间,提高出苗率。种子预处理还能在所有温度下促进芽长。不过,种子预处理对根长的促进作用在 10、15 和 20 °C 时更为明显。水、硫胺素和甘露醇处理的幼苗鲜重和干重在 20 °C 时达到最大值。叶绿素和叶片脯氨酸含量在 20、25 和 30 °C 时达到较高值。克服温度胁迫和促进发芽对大麻的生长发育非常重要。本文得出的结论是,用水、硫胺和甘露醇处理过的大麻种子在低温、最适温度和高温下的生物量值最高,因此这些种子处理方法适用于在发芽和早期生长阶段可能遇到低温或高温的大麻植物。
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引用次数: 0
Rapid synthesis of Silver Nanoparticles by Rheum ribes L Fruit Peels Anticancer and Antimicrobial Effects with Biocompatible Structures 用大黄果皮快速合成银纳米粒子 具有生物相容性结构的抗癌和抗菌效果
Pub Date : 2023-12-11 DOI: 10.15832/ankutbd.1380604
Murat Zor, M. Baran, Duygu Neval Sayin Ipek
Silver nanoparticles (AgNPs) are useful substances with a wide range of uses. utilizing the extract taken from the peels of the Rheum ribes L.(Rr) fruit growing in the Erzurum region, silver nanoparticles were speedily created in this study utilizing a quick, easy, and environmentally friendly technique without harmful stages. In order to evaluate the attributes of the synthesized Rr-AgNPs, FE-SEM or TEM micrographs were utilized to characterize the morphology of the AgNPs. A UV-visible spectrophotometer was used to assess the highest absorbance bands of RR-AgNPs. These data were used to produce RR-AgNPs, which were characterized as having exclusively negative surface charges of -25 mV, a spherical shape, maximum absorbance at 428 nm wavelength, and 96 nm size distribution. The effectiveness of the produced AgNPs for usage in medical applications was assessed using the MTT technique with microdilution. Minimum Inhibition Concentrations of Rr-AgNPs on pathogen strains were found to range from 0.03-0.50 mg/L. Additionally, it was discovered that AgNPs effectively suppressed malignant cells in the investigation for the anticancer effects of AgNPs, with rates of 86.27%, 74.67%, and 73.49%. Healthy cells were not subjected to any inhibitory effects at the same concentrations that caused this result.
银纳米粒子(AgNPs)是一种用途广泛的有用物质。本研究利用从生长在埃尔祖鲁姆地区的大黄果(Rr)果皮中提取的提取物,采用快速、简便和环保的技术快速制造出银纳米粒子,且不经过任何有害阶段。为了评估合成的 Rr-AgNPs 的属性,利用 FE-SEM 或 TEM 显微照片来表征 AgNPs 的形态。紫外可见分光光度计用于评估 RR-AgNPs 的最高吸光度带。利用这些数据制备出了 RR-AgNPs,其特点是表面电荷完全为负,为 -25 mV,呈球形,在 428 nm 波长处有最大吸光度,粒度分布为 96 nm。利用微稀释 MTT 技术评估了制备的 AgNPs 在医疗应用中的有效性。结果发现,Rr-AgNPs 对病原体菌株的最小抑制浓度为 0.03-0.50 mg/L。此外,在研究 AgNPs 的抗癌作用时发现,AgNPs 能有效抑制恶性细胞,抑制率分别为 86.27%、74.67% 和 73.49%。在相同的浓度下,健康细胞没有受到任何抑制作用,从而导致了这一结果。
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引用次数: 0
Drone Larvae Homogenate (Apilarnil) as Natural Remedy: Scientific Review 作为自然疗法的无人机幼虫匀浆(Apilarnil):科学评论
Pub Date : 2023-07-17 DOI: 10.15832/ankutbd.1293015
S. Silici
For centuries, honey bee products such as honey, bee pollen, royal jelly, bee bread (perga), and bee venom have been utilized in natural medicine due to their beneficial properties. A great deal of scientific research has been dedicated to exploring their physico-chemical properties and therapeutic effects. Despite this, drone larvae have not received as much attention from the scientific community. Within a honey bee colony, drones are responsible solely for fertilizing queen bee eggs and consuming food reserves collected by worker honey bees. As a result, beekeepers commonly remove excess drone brood from the hive, which is crucial for preventing and treating varroasis. Lyophilization is the most effective method for preserving drone larvae, and the physicochemical properties of fresh and lyophilized drone larvae were compared. The therapeutic effects of drone larvae, such as androgenic, hepatoprotective, immunostimulatory, and hypolipidemic effects in humans and experimental animals, were summarized. This study aims to summarize current scientific knowledge on drone larvae (apilarnil). The author utilized well-known publication databases like SCOPUS, Google Scholar, and Pub Med to gather research on drone larvae. Furthermore, this review collected information on the chemical composition preservation and bioactive action of drone larvae. Thanks to their high levels of amino acids, fatty acids, vitamins, minerals, and hormones, drone larvae can be considered a potential potency-raising agent.
几个世纪以来,蜂蜜、蜂花粉、蜂王浆、蜂面包(perga)和蜂毒等蜜蜂产品因其有益特性而被用于天然药物。大量科学研究致力于探索它们的物理化学特性和治疗效果。尽管如此,无人机幼虫却没有得到科学界的广泛关注。在蜜蜂蜂群中,无人蜂只负责为蜂王卵受精,并消耗工蜂采集的食物储备。因此,养蜂人通常会清除蜂巢中多余的雄蜂幼虫,这对预防和治疗变异病至关重要。冻干是保存蜂王幼虫最有效的方法,研究人员比较了新鲜蜂王幼虫和冻干蜂王幼虫的理化性质。总结了无人机幼虫对人类和实验动物的治疗作用,如雄激素作用、保肝作用、免疫刺激作用和降血脂作用。本研究旨在总结目前有关无花果幼虫(apilarnil)的科学知识。作者利用 SCOPUS、谷歌学术(Google Scholar)和 Pub Med 等知名出版物数据库收集了有关无针幼虫的研究资料。此外,这篇综述还收集了无人机幼虫的化学成分保存和生物活性作用方面的信息。无人机幼虫含有大量氨基酸、脂肪酸、维生素、矿物质和激素,可被视为一种潜在的增效剂。
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