G. Huang, Yuqi Liang, Qing Xie, Yuanhao Hu, Zhengli Liu, Qiong Zhong, Junlei Chen, Y. Lyu, Quan-Feng Zhang, Dang-Fang Gu, Yong-Qiang Wu, J. Ke
{"title":"Prognostic significance of identification of traditional Chinese medicine syndromes in colorectal cancer","authors":"G. Huang, Yuqi Liang, Qing Xie, Yuanhao Hu, Zhengli Liu, Qiong Zhong, Junlei Chen, Y. Lyu, Quan-Feng Zhang, Dang-Fang Gu, Yong-Qiang Wu, J. Ke","doi":"10.53388/tmr20230212002","DOIUrl":"https://doi.org/10.53388/tmr20230212002","url":null,"abstract":"","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70825506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanli Zhen, Jian Qin, Yufen Li, Jian Zhang, Xiao-Hui Yyu, Ji Li, Dong-kai Wang
Background: Oral ulcer is the most common and easily recurrent disease in stomatology, which influence the patients’ communication, normal dietary, and sleep. Evodia rutaecarpa (ER) is a traditional Chinese herbal medicine recorded in ancient Chinese medical books, which has a medicinal history of more than 2,000 years. Clinically, the application of ER at plantar Yongquan point (KI1) is effective in the treatment of oral ulcer. The purpose of this study was to combine the modern transdermal drug delivery system with traditional Chinese medicine to develop the transdermal absorption patch of ER and apply it to the treatment of oral ulcer at Yongquan point of plantar. Methods: Firstly, the medicinal materials of ER were extracted and the extracted materials were prepared into dispersed ER patch. The formulation and preparation process were screened by orthogonal design method and single factor investigation method. The adhesive and transdermal properties of the patch were used as the evaluation index of the preparation. Secondly, Wistar rats were used as experimental animals to establish a rat model of mouth ulcers. Wistar rats were randomly divided into normal group, model group (A), model group (B), low dose group, medium dose group and high dose group. the efficacy of ER on rat’s oral ulcer model was evaluated through three aspect such as apparent index, pathological index and biochemical index. Results: The patch had suitable adhesion and good skin penetration, which was an effective treatment for oral ulcer. In vivo pharmacodynamic studies, compared with the normal group, the body mass and food intake of rats in each group after modeling decreased, the amount of drinking water increased, the tissue structure of oral mucosa was damaged, and the levels of inflammatory factors (TNF-α, IL-6) and malondialdehyde increased, the levels of anti-inflammatory factors (IL-10), cell growth factor (epidermal growth factor, TGF-β 1 ) and superoxide dismutase decreased. Compared with the model group, the body weight and food intake of each dosing group increased, water consumption decreased, the oral mucosal tissue structure was more complete, the levels of TNF-α, IL-6 and malondialdehyde decreased, the levels of IL-10, epidermal growth factor, TGF-β1 and superoxide dismutase increased, and the changes of various indexes were dose-related. Conclusion: ER patch can inhibit inflammatory reaction, enhance the antioxidant defense ability of the body, and promote the repair of damaged oral mucosa, so as to play an effective role in the treatment of oral ulcer.
{"title":"New therapy for oral ulcer: Evodia rutaecarpa patch combined with acupoint application","authors":"Yanli Zhen, Jian Qin, Yufen Li, Jian Zhang, Xiao-Hui Yyu, Ji Li, Dong-kai Wang","doi":"10.53388/tmr20230305003","DOIUrl":"https://doi.org/10.53388/tmr20230305003","url":null,"abstract":"Background: Oral ulcer is the most common and easily recurrent disease in stomatology, which influence the patients’ communication, normal dietary, and sleep. Evodia rutaecarpa (ER) is a traditional Chinese herbal medicine recorded in ancient Chinese medical books, which has a medicinal history of more than 2,000 years. Clinically, the application of ER at plantar Yongquan point (KI1) is effective in the treatment of oral ulcer. The purpose of this study was to combine the modern transdermal drug delivery system with traditional Chinese medicine to develop the transdermal absorption patch of ER and apply it to the treatment of oral ulcer at Yongquan point of plantar. Methods: Firstly, the medicinal materials of ER were extracted and the extracted materials were prepared into dispersed ER patch. The formulation and preparation process were screened by orthogonal design method and single factor investigation method. The adhesive and transdermal properties of the patch were used as the evaluation index of the preparation. Secondly, Wistar rats were used as experimental animals to establish a rat model of mouth ulcers. Wistar rats were randomly divided into normal group, model group (A), model group (B), low dose group, medium dose group and high dose group. the efficacy of ER on rat’s oral ulcer model was evaluated through three aspect such as apparent index, pathological index and biochemical index. Results: The patch had suitable adhesion and good skin penetration, which was an effective treatment for oral ulcer. In vivo pharmacodynamic studies, compared with the normal group, the body mass and food intake of rats in each group after modeling decreased, the amount of drinking water increased, the tissue structure of oral mucosa was damaged, and the levels of inflammatory factors (TNF-α, IL-6) and malondialdehyde increased, the levels of anti-inflammatory factors (IL-10), cell growth factor (epidermal growth factor, TGF-β 1 ) and superoxide dismutase decreased. Compared with the model group, the body weight and food intake of each dosing group increased, water consumption decreased, the oral mucosal tissue structure was more complete, the levels of TNF-α, IL-6 and malondialdehyde decreased, the levels of IL-10, epidermal growth factor, TGF-β1 and superoxide dismutase increased, and the changes of various indexes were dose-related. Conclusion: ER patch can inhibit inflammatory reaction, enhance the antioxidant defense ability of the body, and promote the repair of damaged oral mucosa, so as to play an effective role in the treatment of oral ulcer.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70826094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lei Xu, Q. Zhu, Yang Zhao, Ming Xiong, Si He, Yao Xu, Chen-Xi Liu, Xiao-Fei Hu
Background: Chloroquine (CQ) is an antimalarial drug that was first synthesized by Hans Andersag, a German scientist, in 1934. Chloroquine has been widely researched and used over the years. The rapid development in the fields of modern science and technology has also contributed to the increase in interest in chloroquine. Hence, it is necessary to comprehensively summarize the research trends to understand the breakthroughs made in the field. Methods: The required data was compiled by analyzing the Web of Science Core Collection (WoSCC) database. The search period for studying global research trends in chloroquine research was set from 2012 to 2021 to ensure a comprehensive analysis over an extended timeframe. Data retrieval was performed on April 4, 2022, focusing on articles and reviews published in English. The retrieval words were: (TS = (chloroquine)) OR (TS = (aralen)). A total of 1,091 reviews and 7,259 articles were retrieved and analyzed .The data obtained from WoSCC was captured and analyzed using VOS viewer (version 1.6.16) and Citesspace (version 5.8.R5). Results: The number of literature reports on chloroquine published in the past 10 years has shown an annual increase. Among the countries, the United States has contributed the highest number of papers and ranks first in terms of both H-index and citation count. The League of European Research Universities is one of the largest research-focused university networks, and Malaria Journal stands out as a prominent journal publishing articles relevant to the field of study. A paper authored by Gautret and Philippe in 2020 achieved the highest citation score globally. The biosynthesis of chloroquine, mechanisms of drug resistance, and drug combinations are receiving growing attention. Conclusion: The research area of chloroquine has been significantly influenced by the American and China. The progress of chloroquine research is further propelled by fruitful collaborations among various countries. Researchers have extensively studied the anti-malarial effect, drug resistance mechanism, and autophagy of chloroquine for the development of a treatment method for COVID-19 based on chloroquine. Bibliometric analysis can be employed to identify hotspots, new directions, and frontiers in the field of chloroquine research.
{"title":"Worldwide research trends on chloroquine: a bibliometric analysis from 2012 to 2021","authors":"Lei Xu, Q. Zhu, Yang Zhao, Ming Xiong, Si He, Yao Xu, Chen-Xi Liu, Xiao-Fei Hu","doi":"10.53388/tmr20230515001","DOIUrl":"https://doi.org/10.53388/tmr20230515001","url":null,"abstract":"Background: Chloroquine (CQ) is an antimalarial drug that was first synthesized by Hans Andersag, a German scientist, in 1934. Chloroquine has been widely researched and used over the years. The rapid development in the fields of modern science and technology has also contributed to the increase in interest in chloroquine. Hence, it is necessary to comprehensively summarize the research trends to understand the breakthroughs made in the field. Methods: The required data was compiled by analyzing the Web of Science Core Collection (WoSCC) database. The search period for studying global research trends in chloroquine research was set from 2012 to 2021 to ensure a comprehensive analysis over an extended timeframe. Data retrieval was performed on April 4, 2022, focusing on articles and reviews published in English. The retrieval words were: (TS = (chloroquine)) OR (TS = (aralen)). A total of 1,091 reviews and 7,259 articles were retrieved and analyzed .The data obtained from WoSCC was captured and analyzed using VOS viewer (version 1.6.16) and Citesspace (version 5.8.R5). Results: The number of literature reports on chloroquine published in the past 10 years has shown an annual increase. Among the countries, the United States has contributed the highest number of papers and ranks first in terms of both H-index and citation count. The League of European Research Universities is one of the largest research-focused university networks, and Malaria Journal stands out as a prominent journal publishing articles relevant to the field of study. A paper authored by Gautret and Philippe in 2020 achieved the highest citation score globally. The biosynthesis of chloroquine, mechanisms of drug resistance, and drug combinations are receiving growing attention. Conclusion: The research area of chloroquine has been significantly influenced by the American and China. The progress of chloroquine research is further propelled by fruitful collaborations among various countries. Researchers have extensively studied the anti-malarial effect, drug resistance mechanism, and autophagy of chloroquine for the development of a treatment method for COVID-19 based on chloroquine. Bibliometric analysis can be employed to identify hotspots, new directions, and frontiers in the field of chloroquine research.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70826189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rui Du, Rong-rong Wang, Peng-Zhen Lei, Charity Ngina Mwangi, Zhijiang Zhou, Chengjuan Liu, Sheng-Jie Huang, Ming Ren, Miaomiao Jiang
Background: Xinsuning capsules is a safe and effective drug against phlegm-heat-disturbed arrhythmia (a type of rapid arrhythmia in traditional Chinese medicine. The clinical manifestations include chest tightness, irritability, vomiting, insomnia, and dreaminess) with no noticeable adverse effects. However, the molecular biological basis of Xinsuning capsules in the treatment of arrhythmia is relatively unexplored. Methods: In order to assess the antiarrhythmic effects of Xinsuning capsules, a rat arrhythmia model was constructed by injecting barium chloride into the tail vein. Metabolomic analysis was performed by UHPLC/Q-Exactive-Orbitrap mass spectrometry. The selection of differential variables in the metabolic data were necessitated fold change ≥ 1.5 or fold change ≤ 0.67, together with P < 0.05. Results: Barium chloride-induced arrhythmias in rats were significantly delayed in beginning and cut short in duration, according to electrocardiogram monitoring. Left ventricles of rats exhibitied significantly higher Na + -K + -ATPase activity after taking Xinsuning capsules compared to the model group. Metabolomic analysis showed that Xinsuning capsules could regulate arachidonic acid, prostaglandin F2a, 15-HETE, 15-HPETE, 12-HETE, 12(13)-DHOME, LTB4, inosine, and hypoxanthine. These metabolomics mainly involved arachidonic acid metabolism, unsaturated fat acid biosynthenosis, and purine metabolism. Conclusion: The therapeutic effects of Xinsuning capsules against arrhythmia had been investigated with metabolomics, providing the basis for further comprehensive research.
{"title":"Untargeted metabolomics analysis reveals the efficacy of Xinsuning capsules in ameliorating arrhythmias in rats","authors":"Rui Du, Rong-rong Wang, Peng-Zhen Lei, Charity Ngina Mwangi, Zhijiang Zhou, Chengjuan Liu, Sheng-Jie Huang, Ming Ren, Miaomiao Jiang","doi":"10.53388/tmr20230523005","DOIUrl":"https://doi.org/10.53388/tmr20230523005","url":null,"abstract":"Background: Xinsuning capsules is a safe and effective drug against phlegm-heat-disturbed arrhythmia (a type of rapid arrhythmia in traditional Chinese medicine. The clinical manifestations include chest tightness, irritability, vomiting, insomnia, and dreaminess) with no noticeable adverse effects. However, the molecular biological basis of Xinsuning capsules in the treatment of arrhythmia is relatively unexplored. Methods: In order to assess the antiarrhythmic effects of Xinsuning capsules, a rat arrhythmia model was constructed by injecting barium chloride into the tail vein. Metabolomic analysis was performed by UHPLC/Q-Exactive-Orbitrap mass spectrometry. The selection of differential variables in the metabolic data were necessitated fold change ≥ 1.5 or fold change ≤ 0.67, together with P < 0.05. Results: Barium chloride-induced arrhythmias in rats were significantly delayed in beginning and cut short in duration, according to electrocardiogram monitoring. Left ventricles of rats exhibitied significantly higher Na + -K + -ATPase activity after taking Xinsuning capsules compared to the model group. Metabolomic analysis showed that Xinsuning capsules could regulate arachidonic acid, prostaglandin F2a, 15-HETE, 15-HPETE, 12-HETE, 12(13)-DHOME, LTB4, inosine, and hypoxanthine. These metabolomics mainly involved arachidonic acid metabolism, unsaturated fat acid biosynthenosis, and purine metabolism. Conclusion: The therapeutic effects of Xinsuning capsules against arrhythmia had been investigated with metabolomics, providing the basis for further comprehensive research.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70827256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To investigate the mechanism of Xuefu Zhuyu decoction in the treatment of diabetes mellitus erectile dysfunction. Methods: Rats with diabetes mellitus erectile dysfunction were developed using streptozocin and randomly assigned into model, low-dose herbal, and high-dose herbal groups. All rats were administered normal saline or the corresponding drugs by oral gavage for 4 weeks. The related indices were detected using enzyme-linked immunosorbent assays, immunohistochemistry, western blotting, and transmission electron microscopy. Results: The levels of lectin-like oxidized low-density protein receptor-1, endothelin-1, NADH oxidase, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in the model group were significantly higher than they were in the mock group but lower than they were in the herbal treatment group. The level of nitric oxide was lower in the model group than was in the mock group but higher than the level in the herbal treatment group. The calcium-sensitive receptor, phospho-protein kinase Cδ/protein kinase Cδ, phosphorylated c-Jun amino-terminal kinase/c-Jun amino-terminal kinase, and phospho-P38 mitogen-activated protein kinase/P38 mitogen-activated protein kinase expression levels in the model group were higher than were in the mock group but lower than were in the herbal treatment group. The structures of the Corpus cavernosum penis endothelial cells were significantly improved in the herbal treatment group than they did in the model group. Conclusion: Xuefu Zhuyu decoction can decrease injury to the endothelium, improve vascular endothelial diastolic and contractive function, and inhibit vascular fibrosis in rats with diabetes mellitus erectile dysfunction. This mechanism may be related to the CaSR/Gq-PLC–PKC pathway.
{"title":"Mechanism of the Xuefu Zhuyu decoction in treating diabetes mellitus erectile dysfunction based on the CaSR/Gq-PLC-PKC pathway","authors":"Chaoli Zhang, Guangqi Li, Li Jiang, Hai-qing Fan, Hui Fang, Yuxin Wu","doi":"10.53388/tmr20230406001","DOIUrl":"https://doi.org/10.53388/tmr20230406001","url":null,"abstract":"Background: To investigate the mechanism of Xuefu Zhuyu decoction in the treatment of diabetes mellitus erectile dysfunction. Methods: Rats with diabetes mellitus erectile dysfunction were developed using streptozocin and randomly assigned into model, low-dose herbal, and high-dose herbal groups. All rats were administered normal saline or the corresponding drugs by oral gavage for 4 weeks. The related indices were detected using enzyme-linked immunosorbent assays, immunohistochemistry, western blotting, and transmission electron microscopy. Results: The levels of lectin-like oxidized low-density protein receptor-1, endothelin-1, NADH oxidase, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in the model group were significantly higher than they were in the mock group but lower than they were in the herbal treatment group. The level of nitric oxide was lower in the model group than was in the mock group but higher than the level in the herbal treatment group. The calcium-sensitive receptor, phospho-protein kinase Cδ/protein kinase Cδ, phosphorylated c-Jun amino-terminal kinase/c-Jun amino-terminal kinase, and phospho-P38 mitogen-activated protein kinase/P38 mitogen-activated protein kinase expression levels in the model group were higher than were in the mock group but lower than were in the herbal treatment group. The structures of the Corpus cavernosum penis endothelial cells were significantly improved in the herbal treatment group than they did in the model group. Conclusion: Xuefu Zhuyu decoction can decrease injury to the endothelium, improve vascular endothelial diastolic and contractive function, and inhibit vascular fibrosis in rats with diabetes mellitus erectile dysfunction. This mechanism may be related to the CaSR/Gq-PLC–PKC pathway.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70826254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The aim of this study is to investigate how Guilu Erxian gum treats osteoporosis by using network pharmacology and related experiments. Methods: The TCMSP database, ETCM database, Chemical Specialties database, and DrugBank database were used to retrieve the potential active ingblueients and corresponding targets of Guilu Erxian gum. Therapeutic targets associated with osteoporosis were obtained from the OMIM and Genecards databases. The network was analyzed using Cytoscape 3.8.0 software to determine topological parameters and identify key active ingblueients and targets. Additionally, the effects of Guilu Erxian gum on osteogenic differentiation of bone marrow mesenchymal stem cells were evaluated through alkaline phosphatase assay and alizarin blue calcium nodule staining. The expression of osteogenesis-related genes (Runx2, Osterix, OPN) was assessed using RT-PCR and Western blot. Results: The analysis revealed that Guilu Erxian gum contained 41 active ingblueients and 236 targets, while there were 5,262 known therapeutic targets for osteoporosis. A protein-protein interaction network diagram was constructed using the String database. Enrichment analysis suggested that Guilu Erxian gum may regulate osteoporosis through various signaling pathways and cellular metabolism. The experiments demonstrated that Guilu Erxian gum could promote osteogenic differentiation of bone marrow mesenchymal stem cells, potentially contributing to the treatment of osteoporosis. Moreover, the results showed that Guilu Erxian gum could increase the expression of osteogenesis-related genes (Runx2, Osterix, OPN). Conclusion: Guilu Erxian gum may have anti-osteoporosis effects by stimulating the osteogenic differentiation of stem cells. This study provides a theoretical basis for further understanding the pharmacological mechanism of Guilu Erxian gum in treating osteoporosis.
{"title":"Study on the mechanism of Guilu Erxian gum in the treatment of osteoporosis based on network pharmacology and cell experiment","authors":"Wei-Wei Ma, Wen-Zhou Huang, Ling-Da Li, Hai Xiang, Hao Lyu, Yong Xiong, Xin Huang","doi":"10.53388/tmr20230704002","DOIUrl":"https://doi.org/10.53388/tmr20230704002","url":null,"abstract":"Background: The aim of this study is to investigate how Guilu Erxian gum treats osteoporosis by using network pharmacology and related experiments. Methods: The TCMSP database, ETCM database, Chemical Specialties database, and DrugBank database were used to retrieve the potential active ingblueients and corresponding targets of Guilu Erxian gum. Therapeutic targets associated with osteoporosis were obtained from the OMIM and Genecards databases. The network was analyzed using Cytoscape 3.8.0 software to determine topological parameters and identify key active ingblueients and targets. Additionally, the effects of Guilu Erxian gum on osteogenic differentiation of bone marrow mesenchymal stem cells were evaluated through alkaline phosphatase assay and alizarin blue calcium nodule staining. The expression of osteogenesis-related genes (Runx2, Osterix, OPN) was assessed using RT-PCR and Western blot. Results: The analysis revealed that Guilu Erxian gum contained 41 active ingblueients and 236 targets, while there were 5,262 known therapeutic targets for osteoporosis. A protein-protein interaction network diagram was constructed using the String database. Enrichment analysis suggested that Guilu Erxian gum may regulate osteoporosis through various signaling pathways and cellular metabolism. The experiments demonstrated that Guilu Erxian gum could promote osteogenic differentiation of bone marrow mesenchymal stem cells, potentially contributing to the treatment of osteoporosis. Moreover, the results showed that Guilu Erxian gum could increase the expression of osteogenesis-related genes (Runx2, Osterix, OPN). Conclusion: Guilu Erxian gum may have anti-osteoporosis effects by stimulating the osteogenic differentiation of stem cells. This study provides a theoretical basis for further understanding the pharmacological mechanism of Guilu Erxian gum in treating osteoporosis.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70827084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng He, Ayesha T Tahir, Sabahat Waris, Wenbo Cheng, Jun Kang
{"title":"Network pharmacology analysis combined with experimental verification of the molecular mechanism of Xihuang pill in treating liver cancer","authors":"Meng He, Ayesha T Tahir, Sabahat Waris, Wenbo Cheng, Jun Kang","doi":"10.53388/tmr20221221002","DOIUrl":"https://doi.org/10.53388/tmr20221221002","url":null,"abstract":"","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70825535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: MicroRNAs and traditional Chinese medicine have emerged as pivotal regulators in the progression of gastric carcinoma. Huashi Jiedu decoction has been clinically used to enhance the quality of life in gastric carcinoma patients. Therefore, the present study aimed to elucidate the involvement of MicroRNAs and Huashi Jiedu decoction in gastric carcinoma metastasis and investigate their regulatory mechanisms in anti-tumor effects. Methods: Subsequently, the effect of Huashi Jiedu decoction on gastric carcinoma cell metastasis and proliferation was verified using cell counting kit-8, flow cytometry, wounding healing, and Transwell assays. LncRNA-p21 knockdown cells were successfully established in BGC-823 and MGC-803 cell lines. Mouse models inoculated with MGC-803 cells were generated, and the cellular morphology in tissues was assessed using hematoxylin staining. Additionally, western blot and immunohistochemistry analysis were performed to evaluate the expression of epithelial-mesenchymal transition-related proteins, with real-time PCR validation conducted in both in vitro and in vivo settings. Results: Network pharmacology revealed a close association between proteins involved in the epithelial-mesenchymal transition process and LncRNA-p21 in gastric carcinoma, consistent with the downregulated expression of E-cadherin, N-cadherin, and Twist following LncRNA-p21 knockdown. Cell counting kit-8 and flow cytometry assays confirmed that Huashi Jiedu decoction exhibited concentration-dependent cytotoxicity, effectively inhibiting the distant metastasis of gastric carcinoma cells in wound healing and invasion experiments. Treatment with Huashi Jiedu decoction resulted in increased expression of E-cadherin, N-cadherin, and LncRNA-p21 in both in vitro and in vivo models, as validated by western blot, real-time PCR, and immunohistochemistry. Conclusion: Collectively, our findings demonstrate that Huashi Jiedu decoction suppresses epithelial-mesenchymal transition and inhibits gastric carcinoma invasion and metastasis both in vivo and in vitro through the knockdown of LncRNA-p21, corroborating the initial findings from our network pharmacology analysis.
{"title":"Huashi Jiedu decoction blocks cell cycle and inhibits EMT of gastric cells via LncRNA-p21","authors":"Xi Pang, Chao Shi, Le-Yin Zhang, Yu-Ting He, Qian-Ran Hong, Wei-Ye Lin, Lei-Tao Sun, Sheng-Liang Qiu","doi":"10.53388/tmr20230602001","DOIUrl":"https://doi.org/10.53388/tmr20230602001","url":null,"abstract":"Background: MicroRNAs and traditional Chinese medicine have emerged as pivotal regulators in the progression of gastric carcinoma. Huashi Jiedu decoction has been clinically used to enhance the quality of life in gastric carcinoma patients. Therefore, the present study aimed to elucidate the involvement of MicroRNAs and Huashi Jiedu decoction in gastric carcinoma metastasis and investigate their regulatory mechanisms in anti-tumor effects. Methods: Subsequently, the effect of Huashi Jiedu decoction on gastric carcinoma cell metastasis and proliferation was verified using cell counting kit-8, flow cytometry, wounding healing, and Transwell assays. LncRNA-p21 knockdown cells were successfully established in BGC-823 and MGC-803 cell lines. Mouse models inoculated with MGC-803 cells were generated, and the cellular morphology in tissues was assessed using hematoxylin staining. Additionally, western blot and immunohistochemistry analysis were performed to evaluate the expression of epithelial-mesenchymal transition-related proteins, with real-time PCR validation conducted in both in vitro and in vivo settings. Results: Network pharmacology revealed a close association between proteins involved in the epithelial-mesenchymal transition process and LncRNA-p21 in gastric carcinoma, consistent with the downregulated expression of E-cadherin, N-cadherin, and Twist following LncRNA-p21 knockdown. Cell counting kit-8 and flow cytometry assays confirmed that Huashi Jiedu decoction exhibited concentration-dependent cytotoxicity, effectively inhibiting the distant metastasis of gastric carcinoma cells in wound healing and invasion experiments. Treatment with Huashi Jiedu decoction resulted in increased expression of E-cadherin, N-cadherin, and LncRNA-p21 in both in vitro and in vivo models, as validated by western blot, real-time PCR, and immunohistochemistry. Conclusion: Collectively, our findings demonstrate that Huashi Jiedu decoction suppresses epithelial-mesenchymal transition and inhibits gastric carcinoma invasion and metastasis both in vivo and in vitro through the knockdown of LncRNA-p21, corroborating the initial findings from our network pharmacology analysis.","PeriodicalId":23192,"journal":{"name":"Traditional Medicine Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135494952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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