Pub Date : 2013-11-27DOI: 10.1097/01.tp.0000438970.80290.d5
M. Wicclair
s from the 12th Congress of the International Society for Organ Donation and Procurement November 21–24, 2013 / Sydney, Australia
2013年11月21日至24日，澳大利亚悉尼，国际器官捐赠与获取学会第12届大会
{"title":"Abstracts from the 12th Congress of the International Society for Organ Donation and Procurement November 21–24, 2013 / Sydney, Australia","authors":"M. Wicclair","doi":"10.1097/01.tp.0000438970.80290.d5","DOIUrl":"https://doi.org/10.1097/01.tp.0000438970.80290.d5","url":null,"abstract":"s from the 12th Congress of the International Society for Organ Donation and Procurement November 21–24, 2013 / Sydney, Australia","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"1 1","pages":"S165–S280"},"PeriodicalIF":0.0,"publicationDate":"2013-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87343073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-09-27DOI: 10.1097/tp.0b013e3182a7ab68
M. Vantyghem, D. Quintin, W. Karrouz, J. Hurtevent, V. Raverdy, R. Caiazzo, C. Noel, J. Kerr-Conte, F. Pattou, Shari Messinger-Cayetano, K. Rouskas, R. Alejandro, M. Rickels, F. Barton
Objective: Long-term benefit-risk ratio of islet transplantation rem unclear. We explored the evolution of peripheral and autonomic ne pathy during 5 years after islet transplantation with the Edmonton pr col in type 1 diabetic patients. Patients and Methods: Twenty-one patients (13 islet-alone 8 islet-after-kidney) were enrolled in this prospective cohort study. transplantation consisted of 2 or 3 sequential infusions with IL2r sirolimus tacrolimus immunosuppression. All patients underwent logical evaluation, continuous blood pressure and continuous gluc monitoring (CGM), lower-limb electrophysiological testing and car vascular autonomic testing (R-R variation with paced breathing, Vals ratio, postural heart rate and blood pressure changes) before transp tation and yearly during 5 years. Outcomes were analyzed in inten to treat. Results: At 5 years, islet remained functional in 18 patients (85 Ten patients (48%) were insulin-independent with a median (I HbA1c at 6.0 (5.8–6.7) % vs. 7.8 (6.9-8.3) % in those requiring ins (p<0.001). The medians of sensory action potential (p<0.05) and sensory and motor nerve conduction velocities (p<0.01) improved tween 0 and 5 years. All 4 parameters significantly correlated negati with mean glucose / CGM and all outcomes except sensory nerve duction velocity correlated negatively with triglycerides (p≤0.01). S sory conduction velocity correlated negatively with glucose variab (SD) / CGM (p<0.01). Tacrolimus levels negatively correlated with m conduction parameters (p≤0.02). All four parameters correlated p tively with ß score or post-prandial C-peptide level (p<0.05). Cardio cular reflex testing did not change over the 5–year follow-up. Conclusion: islet-alone or after-kidney transplantation improved nificantly sensory nerve conduction parameters but not autonomic n ropathy after 5 years. Mean glucose was the main factor associated this improvement. (ClinicalTrial.gov: NCT00446264 / NCT01123187).
{"title":"IPITA 2013 Abstracts Supplement","authors":"M. Vantyghem, D. Quintin, W. Karrouz, J. Hurtevent, V. Raverdy, R. Caiazzo, C. Noel, J. Kerr-Conte, F. Pattou, Shari Messinger-Cayetano, K. Rouskas, R. Alejandro, M. Rickels, F. Barton","doi":"10.1097/tp.0b013e3182a7ab68","DOIUrl":"https://doi.org/10.1097/tp.0b013e3182a7ab68","url":null,"abstract":"Objective: Long-term benefit-risk ratio of islet transplantation rem unclear. We explored the evolution of peripheral and autonomic ne pathy during 5 years after islet transplantation with the Edmonton pr col in type 1 diabetic patients. Patients and Methods: Twenty-one patients (13 islet-alone 8 islet-after-kidney) were enrolled in this prospective cohort study. transplantation consisted of 2 or 3 sequential infusions with IL2r sirolimus tacrolimus immunosuppression. All patients underwent logical evaluation, continuous blood pressure and continuous gluc monitoring (CGM), lower-limb electrophysiological testing and car vascular autonomic testing (R-R variation with paced breathing, Vals ratio, postural heart rate and blood pressure changes) before transp tation and yearly during 5 years. Outcomes were analyzed in inten to treat. Results: At 5 years, islet remained functional in 18 patients (85 Ten patients (48%) were insulin-independent with a median (I HbA1c at 6.0 (5.8–6.7) % vs. 7.8 (6.9-8.3) % in those requiring ins (p<0.001). The medians of sensory action potential (p<0.05) and sensory and motor nerve conduction velocities (p<0.01) improved tween 0 and 5 years. All 4 parameters significantly correlated negati with mean glucose / CGM and all outcomes except sensory nerve duction velocity correlated negatively with triglycerides (p≤0.01). S sory conduction velocity correlated negatively with glucose variab (SD) / CGM (p<0.01). Tacrolimus levels negatively correlated with m conduction parameters (p≤0.02). All four parameters correlated p tively with ß score or post-prandial C-peptide level (p<0.05). Cardio cular reflex testing did not change over the 5–year follow-up. Conclusion: islet-alone or after-kidney transplantation improved nificantly sensory nerve conduction parameters but not autonomic n ropathy after 5 years. Mean glucose was the main factor associated this improvement. (ClinicalTrial.gov: NCT00446264 / NCT01123187).","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"22 1","pages":"S1–S155"},"PeriodicalIF":0.0,"publicationDate":"2013-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83476402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Recent years have witnessed remarkable expansion of the knowledge on reciprocal regulatory effect between various immune/inflammatory cells and T helper (Th) cell subsets including Th1, Th2, Th9, Th17, Th22, and follicular T-helper (Tfh) and regulatory T cells (Treg). Uncommitted naBve Th cells can be induced to differentiate to specific lineages according to the local cytokine milieu, towards Th or Treg phenotypes (1). The significance of Th17 immunity in acute, chronic and antibody-mediated allograft rejection is well known (2). However, Treg associated regulatory mechanisms have been implicated in tipping the balance in favor of tolerance rather than rejection (3). This review article summarizes the current knowledge on the contribution of T-helper subset cells in allograft rejection. The authors use a xenogeneic transplantation model to show the mechanisms by which Th1 cells participate in a allograft rejection. It also highlights that IL-12 or IFN-F knockout recipients showed faster acute vascular rejection than wildtype mice. Th2 cytokines inhibit Th1 responses and, as a result, can delay and even prevent acute rejection but seem to dominate during chronic rejection. The article also presents an extensive discussion of the involvement of Th17 in acute and chronic allograft rejection. The hallmark of Th17 cell-mediated allograft rejection is IL-17_s ability to recruit neutrophils which are one of the first inflammatory effector cells to infiltrate the allograft after transplantation and cause allograft damage. In addition a link between Th17 and alloimmunity particularly in the context of chronic lung allograft rejection is discussed. The final section of the review discusses the regulation of Th subsets by CD4+Foxp3+ Tregs in transplantation. It is well established that Tregs appear to be the Bmaster regulators[ that induce and maintain transplantation tolerance in experimental and clinical transplantation possibly by suppressing both Th1 and Th2 clones. Interestingly Th17 cells have the tendency to resist Treg-mediated suppression. The authors advocated that further studies are still needed to unravel the underlying mechanisms of these cells in the complex contexts of allograft rejection and tolerance and would facilitate devising strategies to achieve tolerance in the clinic.
{"title":"Research Highlights and Editors’ Picks","authors":"Kumar Kuna, Chougule, MJ Elliott, De Coppi, Speggiorin, JJ Hsuan, Lowdell, Mw, Birchall","doi":"10.1097/01.tp.0000431075.93885.89","DOIUrl":"https://doi.org/10.1097/01.tp.0000431075.93885.89","url":null,"abstract":"Recent years have witnessed remarkable expansion of the knowledge on reciprocal regulatory effect between various immune/inflammatory cells and T helper (Th) cell subsets including Th1, Th2, Th9, Th17, Th22, and follicular T-helper (Tfh) and regulatory T cells (Treg). Uncommitted naBve Th cells can be induced to differentiate to specific lineages according to the local cytokine milieu, towards Th or Treg phenotypes (1). The significance of Th17 immunity in acute, chronic and antibody-mediated allograft rejection is well known (2). However, Treg associated regulatory mechanisms have been implicated in tipping the balance in favor of tolerance rather than rejection (3). This review article summarizes the current knowledge on the contribution of T-helper subset cells in allograft rejection. The authors use a xenogeneic transplantation model to show the mechanisms by which Th1 cells participate in a allograft rejection. It also highlights that IL-12 or IFN-F knockout recipients showed faster acute vascular rejection than wildtype mice. Th2 cytokines inhibit Th1 responses and, as a result, can delay and even prevent acute rejection but seem to dominate during chronic rejection. The article also presents an extensive discussion of the involvement of Th17 in acute and chronic allograft rejection. The hallmark of Th17 cell-mediated allograft rejection is IL-17_s ability to recruit neutrophils which are one of the first inflammatory effector cells to infiltrate the allograft after transplantation and cause allograft damage. In addition a link between Th17 and alloimmunity particularly in the context of chronic lung allograft rejection is discussed. The final section of the review discusses the regulation of Th subsets by CD4+Foxp3+ Tregs in transplantation. It is well established that Tregs appear to be the Bmaster regulators[ that induce and maintain transplantation tolerance in experimental and clinical transplantation possibly by suppressing both Th1 and Th2 clones. Interestingly Th17 cells have the tendency to resist Treg-mediated suppression. The authors advocated that further studies are still needed to unravel the underlying mechanisms of these cells in the complex contexts of allograft rejection and tolerance and would facilitate devising strategies to achieve tolerance in the clinic.","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"119 1","pages":"&NA;"},"PeriodicalIF":0.0,"publicationDate":"2013-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86183989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.1097/00007890-201211271-00702
J. Carbone, N. D. Pozo, A. Gallego, N. Lanio, C. Rodríguez, J. Rodríguez-Molina, J. Navarro, J. Palomo, J. Fernández‐Yáñez, A. Villa, P. Muñoz, M. Ruiz, J. Hortal, K. Kotsch, F. Kern, E. Fernandez-cruz, E. Sarmiento
Regulatory T cells (Tregs) were identified several years ago and are key in controlling autoimmune diseases and limiting immune responses to foreign antigens. Imaging of the human sodium/iodide symporter via Single Photon Emission Computed Tomography (SPECT) has been used to image various cell types in vivo . This study addresses whether SPECT/CT imaging can be used to visualise the migratory pattern of Tregs in vivo . Murine Treg lines were retrovirally transduced with a construct encoding for the human Sodium Iodide Symporter (NIS). NIS expressing self-specific Tregs were specifically radiolabelled in vitro with Technetium-99m pertechnetate ( 99m TcO 4- ) and exposure of these cells to radioactivity did not affect cell viability, phenotype or function. In addition adoptively transferred Treg-NIS cells were imaged in vivo in mice by SPECT/CT using 99m TcO 4- . After 24-hours Treg-NIS cells were observed in the spleen and their localisation was further confirmed by organ-biodistribution studies and flow cytometry analysis. Moreover, we have demonstrated that this method of imaging can be utilised to image migration of Tregs with direct and indirect allo-specificity in a skin transplant model. The data presented here suggests that SPECT/CT can be utilised in preclinical imaging studies of adoptively transferred Tregs without affecting Treg function and viability thereby allowing longitudinal studies within disease models. study. heart Clinical follow-up: 6 months. intravenous antimicrobial therapy, with positive clinical compromise. Study points: pre-trasplant, day-7 and day-30 after transplant. The immunological study included humoral and cellular immunity markers of : adaptive and innate immunity: Serum IgG, IgA, IgM, IgG subclasses, C3, C4, factor B (Nephelometry), manose binding lectin (ELISA), specific antibodies after vaccination (anti-pneumococcal polysaccharide [anti-PPS], anti-HBs, [ELISA]), natural specific antibodies without vaccination (anti-cytomegalovirus, anti-haemophilus influenzae type B, anti-salmonella typhi, anti-varicella zoster and T, B, NK lymphocyte subsets (flow-cytometry). Results: 154 patients were analysed. (38.3%) severe infections during follow-up. Univariate single marker analysis: Patients who disclosed: Lower levels of IgG (day 7, p< 0.001), C4 (day 7, p=0.016), IgG (day 30, p=0.007), IgA (day 30, p=0.016), anti-PPS (day 30, p=0.003) and lower absolute counts of NK CD3-CD16/CD56+ (day 7, p=0.017), T-CD3+ (day 30, p=0.008), T-CD4+ (day 30, p=0.012) and T-CD8+ lymphocytes (day 30, p=0.003). of immune profiles severe infection risk: Day 7: IgG< mg/dl + cells/uL (OR 3.327, p< 0.001, specificity 88.5%, PPV 72%); day 30: IgG<
{"title":"Immune Profiles to Detect Heart Recipients at Risk of Development of Severe Infection","authors":"J. Carbone, N. D. Pozo, A. Gallego, N. Lanio, C. Rodríguez, J. Rodríguez-Molina, J. Navarro, J. Palomo, J. Fernández‐Yáñez, A. Villa, P. Muñoz, M. Ruiz, J. Hortal, K. Kotsch, F. Kern, E. Fernandez-cruz, E. Sarmiento","doi":"10.1097/00007890-201211271-00702","DOIUrl":"https://doi.org/10.1097/00007890-201211271-00702","url":null,"abstract":"Regulatory T cells (Tregs) were identified several years ago and are key in controlling autoimmune diseases and limiting immune responses to foreign antigens. Imaging of the human sodium/iodide symporter via Single Photon Emission Computed Tomography (SPECT) has been used to image various cell types in vivo . This study addresses whether SPECT/CT imaging can be used to visualise the migratory pattern of Tregs in vivo . Murine Treg lines were retrovirally transduced with a construct encoding for the human Sodium Iodide Symporter (NIS). NIS expressing self-specific Tregs were specifically radiolabelled in vitro with Technetium-99m pertechnetate ( 99m TcO 4- ) and exposure of these cells to radioactivity did not affect cell viability, phenotype or function. In addition adoptively transferred Treg-NIS cells were imaged in vivo in mice by SPECT/CT using 99m TcO 4- . After 24-hours Treg-NIS cells were observed in the spleen and their localisation was further confirmed by organ-biodistribution studies and flow cytometry analysis. Moreover, we have demonstrated that this method of imaging can be utilised to image migration of Tregs with direct and indirect allo-specificity in a skin transplant model. The data presented here suggests that SPECT/CT can be utilised in preclinical imaging studies of adoptively transferred Tregs without affecting Treg function and viability thereby allowing longitudinal studies within disease models. study. heart Clinical follow-up: 6 months. intravenous antimicrobial therapy, with positive clinical compromise. Study points: pre-trasplant, day-7 and day-30 after transplant. The immunological study included humoral and cellular immunity markers of : adaptive and innate immunity: Serum IgG, IgA, IgM, IgG subclasses, C3, C4, factor B (Nephelometry), manose binding lectin (ELISA), specific antibodies after vaccination (anti-pneumococcal polysaccharide [anti-PPS], anti-HBs, [ELISA]), natural specific antibodies without vaccination (anti-cytomegalovirus, anti-haemophilus influenzae type B, anti-salmonella typhi, anti-varicella zoster and T, B, NK lymphocyte subsets (flow-cytometry). Results: 154 patients were analysed. (38.3%) severe infections during follow-up. Univariate single marker analysis: Patients who disclosed: Lower levels of IgG (day 7, p< 0.001), C4 (day 7, p=0.016), IgG (day 30, p=0.007), IgA (day 30, p=0.016), anti-PPS (day 30, p=0.003) and lower absolute counts of NK CD3-CD16/CD56+ (day 7, p=0.017), T-CD3+ (day 30, p=0.008), T-CD4+ (day 30, p=0.012) and T-CD8+ lymphocytes (day 30, p=0.003). of immune profiles severe infection risk: Day 7: IgG< mg/dl + cells/uL (OR 3.327, p< 0.001, specificity 88.5%, PPV 72%); day 30: IgG<","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"24 1","pages":"380"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75508298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.1097/00007890-201211271-00751
C. Lee, A. Thorat, W. Lee
{"title":"Endovascular Treatment for the Pseudoaneurysms Arising from Hepatic Artery after Liver Transplantation","authors":"C. Lee, A. Thorat, W. Lee","doi":"10.1097/00007890-201211271-00751","DOIUrl":"https://doi.org/10.1097/00007890-201211271-00751","url":null,"abstract":"","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"76 1","pages":"403"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85373884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-11-01DOI: 10.1097/00007890-201211271-00845
M. Oberbarnscheidt, Q. Zeng, A. Williams, R. Hoffman, D. Rothstein, Fadi G Lakkis
after is a response to non-physiological cell death (‚danger‘) rather than to allogeneic non-self. we tested the alternate hypothesis that the innate immune system distinguishes between self and allogeneic non-self, causing DC maturation and activation of the adaptive immune response. Methods: T, B and NK cell-deficient B6 Rag -/- gc -/- or WT CX3CR1- GFP +/- reporter mice in monocyte lineage cells express GFP syngeneic (B6) or allogeneic (Balb/c) vascularized heart grafts. Graft-infiltrating cells analyzed 1, 3, 5, 10, 21 and 42 days after transplantation (Tx) and the function of host-derived DC tested ex and in vivo Results: in the graft by day after were host monocytes (mono-DC). numbers (2-5 fold) in allo than syn grafts at early time points (days 1-10) and at later time points (days 21 42) in allografts. Mono-DC allografts higher proportion of mature cells (MHCII + CD80 + ), IL-12p40, allogeneic T cells in the MLR, endotoxin-free antigen (OVA) to TCR-tg CD4 + (OT-II) and CD8 + (OT-I) T cells early and late after Tx. In vivo depletion of monocytes with clodronate liposomes abrogated the proliferation of alloreactive 1H3.1 TCR-tg CD4 + T cells transferred to allograft recipients. Depletion of host CD11b + cells by diphtheria toxin (DT) CD11b-DTR bone marrow chimeras dramatically reduced T cell accumulation in heart allografts examined on day 15 after Tx while DT-treated WT bone marrow chimeras rejected their allografts by day 10 with evidence of heavy T cell infiltration. self and allogeneic Multimers of soluble peptide-major histocompatibilty complex (pMHC) molecules are used in basic as well as in clinic immunology. They allow a specific visualization, phenotype characterization and isolation of antigen- specific T cells from ex vivo samples. Adoptive transfer of antigen-specific T cells sorted by pMHC multimers is an effective therapeutic strategy for treatment of patients with malignancies or infectious diseases after transplantation. A new reversible pMHC multimer technology, called pMHC Histamer technology , was developed enabling a specific detection and isolation of antiviral T cells from peripheral blood mononuclear cells. The HLA-A02/CMVpp65 and HLA-A01/ADV5_Hexon Histamers were generated by coupling 6xHis-tagged pMHC molecules onto cobalt-magnetic beads. The specificity and sensitivity of the magnetic bead-based Histamers was evaluated by flow cytometry. Sorting of antiviral CD8 + cytotoxic T cells (CTLs) was performed by magnetic cell separation, followed by the monomerization of the pMHC Histamers in the presence of L-histidine. Sorted T cells were analyzed in phenotypical and functional assays. The reversible Histamers showed superior specificity and sensitivity (up to 99.5%). Antigen-specific T cells isolated by this technology gave an excellent purity of up to 99.6%. A rapid and complete disassembly of the T-cell surface-bound pMHC Histamers followed by the subsequent dissociation of the pMHC
{"title":"Innate Recognition of Allogeneic Non-Self Induces Monocyte Differentiation to Mature Dendritic Cells In Vivo","authors":"M. Oberbarnscheidt, Q. Zeng, A. Williams, R. Hoffman, D. Rothstein, Fadi G Lakkis","doi":"10.1097/00007890-201211271-00845","DOIUrl":"https://doi.org/10.1097/00007890-201211271-00845","url":null,"abstract":"after is a response to non-physiological cell death (‚danger‘) rather than to allogeneic non-self. we tested the alternate hypothesis that the innate immune system distinguishes between self and allogeneic non-self, causing DC maturation and activation of the adaptive immune response. Methods: T, B and NK cell-deficient B6 Rag -/- gc -/- or WT CX3CR1- GFP +/- reporter mice in monocyte lineage cells express GFP syngeneic (B6) or allogeneic (Balb/c) vascularized heart grafts. Graft-infiltrating cells analyzed 1, 3, 5, 10, 21 and 42 days after transplantation (Tx) and the function of host-derived DC tested ex and in vivo Results: in the graft by day after were host monocytes (mono-DC). numbers (2-5 fold) in allo than syn grafts at early time points (days 1-10) and at later time points (days 21 42) in allografts. Mono-DC allografts higher proportion of mature cells (MHCII + CD80 + ), IL-12p40, allogeneic T cells in the MLR, endotoxin-free antigen (OVA) to TCR-tg CD4 + (OT-II) and CD8 + (OT-I) T cells early and late after Tx. In vivo depletion of monocytes with clodronate liposomes abrogated the proliferation of alloreactive 1H3.1 TCR-tg CD4 + T cells transferred to allograft recipients. Depletion of host CD11b + cells by diphtheria toxin (DT) CD11b-DTR bone marrow chimeras dramatically reduced T cell accumulation in heart allografts examined on day 15 after Tx while DT-treated WT bone marrow chimeras rejected their allografts by day 10 with evidence of heavy T cell infiltration. self and allogeneic Multimers of soluble peptide-major histocompatibilty complex (pMHC) molecules are used in basic as well as in clinic immunology. They allow a specific visualization, phenotype characterization and isolation of antigen- specific T cells from ex vivo samples. Adoptive transfer of antigen-specific T cells sorted by pMHC multimers is an effective therapeutic strategy for treatment of patients with malignancies or infectious diseases after transplantation. A new reversible pMHC multimer technology, called pMHC Histamer technology , was developed enabling a specific detection and isolation of antiviral T cells from peripheral blood mononuclear cells. The HLA-A02/CMVpp65 and HLA-A01/ADV5_Hexon Histamers were generated by coupling 6xHis-tagged pMHC molecules onto cobalt-magnetic beads. The specificity and sensitivity of the magnetic bead-based Histamers was evaluated by flow cytometry. Sorting of antiviral CD8 + cytotoxic T cells (CTLs) was performed by magnetic cell separation, followed by the monomerization of the pMHC Histamers in the presence of L-histidine. Sorted T cells were analyzed in phenotypical and functional assays. The reversible Histamers showed superior specificity and sensitivity (up to 99.5%). Antigen-specific T cells isolated by this technology gave an excellent purity of up to 99.6%. A rapid and complete disassembly of the T-cell surface-bound pMHC Histamers followed by the subsequent dissociation of the pMHC","PeriodicalId":23474,"journal":{"name":"Transplantation Journal","volume":"65 1","pages":"446"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83112618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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