Pub Date : 2017-12-14DOI: 10.1615/INTERJENERCLEANENV.2018020888
Shivam Patel, A. Usmani
{"title":"Hemodynamics of a Cerebral Aneurysm under Rest and Exercise Conditions","authors":"Shivam Patel, A. Usmani","doi":"10.1615/INTERJENERCLEANENV.2018020888","DOIUrl":"https://doi.org/10.1615/INTERJENERCLEANENV.2018020888","url":null,"abstract":"","PeriodicalId":23678,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Biotechnology and Bioengineering","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79020209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dietary Ergosan as a Supplemental Nutrient on Growth Performance, and Stress in Zebrafish (Danio Rerio)","authors":"E. Ahmadifar","doi":"10.17758/urst.u1117216","DOIUrl":"https://doi.org/10.17758/urst.u1117216","url":null,"abstract":"","PeriodicalId":23678,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Biotechnology and Bioengineering","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86621646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inulinase from Aspergillus niger was covalently immobilized on magnetic nanoparticles (MNPs/Fe3O4) covered with soy protein isolate (SPI/Fe3O4) functionalized by bovine serum albumin (BSA) nanoparticles. MNPs are promising enzyme carriers because they separate easily under external magnetic fields and have enhanced immobilized enzyme reusability. As MNPs aggregate simply, surface coating strategy was employed. SPI functionalized by BSA was a suitable candidate for nanomagnetite coating due to its superior biocompatibility and hydrophilicity. Fe3O4@SPI-BSA nanoparticles were synthesized as a novel carrier with narrow particle size distribution. Step by step fabrication monitoring of Fe3O4@SPI-BSA nanoparticles was performed using field emission scanning electron microscopy and dynamic light scattering. The results illustrated that nanomagnetite with the spherical morphology was well monodispersed with the diameter of about 35 nm. The average size of the SPI-BSA nanoparticles was 80 to 90 nm, and their zeta potential was around −34 mV. Finally, the mean diameter of fabricated Fe3O4@SPI-BSA NPs was less than 120 nm. Inulinase enzyme from Aspergillus niger was covalently immobilized through gluteraldehyde on Fe3O4@SPI-BSA nanoparticles successfully. Fourier transform infrared spectra and field emission scanning electron microscopy images provided sufficient proof for the enzyme immobilization on the nanoparticles with 80% enzyme loading.
{"title":"Inulinase Immobilization on Functionalized Magnetic Nanoparticles Prepared with Soy Protein Isolate Conjugated Bovine Serum Albumin for High Fructose Syrup Production","authors":"H. Torabizadeh, M. Mikani","doi":"10.5281/ZENODO.1131957","DOIUrl":"https://doi.org/10.5281/ZENODO.1131957","url":null,"abstract":"Inulinase from Aspergillus niger was covalently immobilized on magnetic nanoparticles (MNPs/Fe3O4) covered with soy protein isolate (SPI/Fe3O4) functionalized by bovine serum albumin (BSA) nanoparticles. MNPs are promising enzyme carriers because they separate easily under external magnetic fields and have enhanced immobilized enzyme reusability. As MNPs aggregate simply, surface coating strategy was employed. SPI functionalized by BSA was a suitable candidate for nanomagnetite coating due to its superior biocompatibility and hydrophilicity. Fe3O4@SPI-BSA nanoparticles were synthesized as a novel carrier with narrow particle size distribution. Step by step fabrication monitoring of Fe3O4@SPI-BSA nanoparticles was performed using field emission scanning electron microscopy and dynamic light scattering. The results illustrated that nanomagnetite with the spherical morphology was well monodispersed with the diameter of about 35 nm. The average size of the SPI-BSA nanoparticles was 80 to 90 nm, and their zeta potential was around −34 mV. Finally, the mean diameter of fabricated Fe3O4@SPI-BSA NPs was less than 120 nm. Inulinase enzyme from Aspergillus niger was covalently immobilized through gluteraldehyde on Fe3O4@SPI-BSA nanoparticles successfully. Fourier transform infrared spectra and field emission scanning electron microscopy images provided sufficient proof for the enzyme immobilization on the nanoparticles with 80% enzyme loading.","PeriodicalId":23678,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Biotechnology and Bioengineering","volume":"16 1","pages":"546-553"},"PeriodicalIF":0.0,"publicationDate":"2017-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73884230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Al-akeel, T. A. El-kersh, Y. Al-Sheikh, Z. Alahmadey
Pregnancy represents a risk factor in the occurrence of vulvovaginal candidiasis. To investigate the prevalence rate of vaginal carriage of Candida species in Saudi pregnant and non-pregnant women, high vaginal swab (HVS) specimens (707) were examined by direct microscopy (10% KOH and Giemsa staining) and parallel cultured on Sabouraud Dextrose Agar (SDA) as well as on “CHROM agar Candida” medium. As expected, Candida-positive cultures were frequently observed in pregnant-test group (24%) than in non-pregnant group (17%). The frequency of culture positive was correlated to pregnancy (P=0.047), parity (P=0.001), use of contraceptive (P=0.146), or antibiotics (P=0.128), and diabetic-patients (P<0.0001). Out of 707 HVS examined specimens, 157 specimens were yeast-positive culture (22%) on Sabouraud Dextrose Agar or “CHROM agar Candida”. In comparison, the sensitivities of the direct 10% KOH and the Giemsa stain microscopic examination methods were 84% (132/157) and 95% (149/157) respectively but both with 100% specificity. As for the identity of recovered 157 yeast isolates, based on API 20C biotype carbohydrate assimilation, germ tube and chlamydospore formation, C. albicans and C. glabrata constitute 80.3 and 12.7% respectively. Whereas rates of C. tropicalis, C. kefyr, C. famata or C. utilis were 2.6, 1.3, and 0.6% respectively. Sachromyces cerevisiae and Rhodotorula mucilaginosa yeasts were also encountered at a frequency of 1.3 and 0.6% respectively. Finally, among all recovered 157 yeast-isolates, strains resistant to ketoconazole were not detected, whereas 5% of the C. albicans and as high as 55% of the non-albicans yeast isolates (majority C. glabrata) showed resistance to fluconazole. Our findings may prove helpful for continuous determination of the existing vaginal candidiasis causative species during pregnancy, its lab-diagnosis and/or control and possible measures to minimize the incidence of the disease-associated pre-term delivery.
妊娠是发生外阴阴道念珠菌病的一个危险因素。为了调查沙特孕妇和非孕妇阴道携带念珠菌的患病率，采用直接显微镜(10% KOH和Giemsa染色)检查高阴道拭子(HVS)标本(707)，并在Sabouraud Dextrose Agar (SDA)和“CHROM琼脂念珠菌”培养基上平行培养。正如预期的那样，妊娠试验组(24%)比非妊娠组(17%)经常观察到念珠菌阳性培养。培养阳性频率与妊娠(P=0.047)、胎次(P=0.001)、使用避孕药(P=0.146)或抗生素(P=0.128)、糖尿病患者(P<0.0001)相关。在707个HVS检测标本中，157个标本在Sabouraud葡萄糖琼脂或“CHROM琼脂念珠菌”上培养呈酵母阳性(22%)。10% KOH直接镜检和Giemsa染色镜检的敏感性分别为84%(132/157)和95%(149/157)，特异性均为100%。对于回收的157株酵母菌，从API 20C生物型碳水化合物同化、胚管和衣孢子形成等方面进行鉴定，白色念珠菌和光面念珠菌分别占80.3%和12.7%。而热带恙螨、kefyr恙螨、famata恙螨和utilis恙螨的感染率分别为2.6%、1.3%和0.6%。酿酒sacroomyces cerevisiae和粘胶红酵母(Rhodotorula mucilaginosa)的频率也分别为1.3%和0.6%。最后，在所有回收的157株酵母菌分离株中，未检出对酮康唑耐药的菌株，而5%的白色念珠菌和高达55%的非白色念珠菌分离株(大多数为光滑念珠菌)对氟康唑耐药。我们的研究结果可能有助于持续确定妊娠期间阴道念珠菌病的致病种类，其实验室诊断和/或控制以及可能的措施，以尽量减少疾病相关早产的发生率。
{"title":"Prevalence and comparison for detection methods of Candida species in vaginal specimens from pregnant and non pregnant Saudi women","authors":"R. Al-akeel, T. A. El-kersh, Y. Al-Sheikh, Z. Alahmadey","doi":"10.5897/AJMR12.1979","DOIUrl":"https://doi.org/10.5897/AJMR12.1979","url":null,"abstract":"Pregnancy represents a risk factor in the occurrence of vulvovaginal candidiasis. To investigate the prevalence rate of vaginal carriage of Candida species in Saudi pregnant and non-pregnant women, high vaginal swab (HVS) specimens (707) were examined by direct microscopy (10% KOH and Giemsa staining) and parallel cultured on Sabouraud Dextrose Agar (SDA) as well as on “CHROM agar Candida” medium. As expected, Candida-positive cultures were frequently observed in pregnant-test group (24%) than in non-pregnant group (17%). The frequency of culture positive was correlated to pregnancy (P=0.047), parity (P=0.001), use of contraceptive (P=0.146), or antibiotics (P=0.128), and diabetic-patients (P<0.0001). Out of 707 HVS examined specimens, 157 specimens were yeast-positive culture (22%) on Sabouraud Dextrose Agar or “CHROM agar Candida”. In comparison, the sensitivities of the direct 10% KOH and the Giemsa stain microscopic examination methods were 84% (132/157) and 95% (149/157) respectively but both with 100% specificity. As for the identity of recovered 157 yeast isolates, based on API 20C biotype carbohydrate assimilation, germ tube and chlamydospore formation, C. albicans and C. glabrata constitute 80.3 and 12.7% respectively. Whereas rates of C. tropicalis, C. kefyr, C. famata or C. utilis were 2.6, 1.3, and 0.6% respectively. Sachromyces cerevisiae and Rhodotorula mucilaginosa yeasts were also encountered at a frequency of 1.3 and 0.6% respectively. Finally, among all recovered 157 yeast-isolates, strains resistant to ketoconazole were not detected, whereas 5% of the C. albicans and as high as 55% of the non-albicans yeast isolates (majority C. glabrata) showed resistance to fluconazole. Our findings may prove helpful for continuous determination of the existing vaginal candidiasis causative species during pregnancy, its lab-diagnosis and/or control and possible measures to minimize the incidence of the disease-associated pre-term delivery.","PeriodicalId":23678,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Biotechnology and Bioengineering","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78219395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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