Pub Date : 2020-01-09DOI: 10.4303/2471-9455/8/236068
Shalini Tv, Gangadharan Gg, Sriranjini Jaideep, Asn Seshasayee, A. Pandit
Prakriti (body –mind constitution of an individual) is a conventional, customized and unique the understanding of which is essential for the personalized medicine described in Ayurveda, Indian System of Medicine. Based on the Doshas( functional, bio humoral unit in the body) individuals are categorized into three major prakriti- Vata, Pitta and Kapha. The human gut micro biome hosts plenty of highly diverse and metabolically active microorganisms, mainly dominated by the bacteria, which are known to influence the physiology of an individual. Few researches have shown the correlation between the prakriti and the biochemical parameters. In this study, an attempt was made to explore any correlation between the prakriti (phenotype of an individual) with the Genetic makeup of the gut micro biome in healthy individuals. Materials and methods: 270 multi ethnic, healthy volunteers of both the sex with the age group between 18 to 40 years, with no history of antibiotics in last 6 months were recruited into three groups of Vata, Pitta and Kapha. The Prakriti of the individual was determined using Ayusoft, a software designed by CDAC, Pune, India. The volunteers were subjected to initial screening for the assessment of their height, weight, Body Mass Index, Vital signs and Blood investigations to ensure they are healthy. The stool sample of the recruited volunteers were collected as per the standard operating procedure developed and the bacterial DNA was isolated using Qiagen kits. The extracted DNA was subjected to 16s rRNA sequencing using the Illumina kits. The sequencing libraries targeting the variable V3 and V4 regions of the 16s rRNA gene. Paired sequencing was done on MiSeq system and data were analyzed using the CLC Genomics workbench 11. Results: The 16s rRNA sequencing of V3 and V4 region showed diverse pattern in both the oral and stool microbial DNA. The study did not reveal any specific pattern of bacterial flora amongst the prakriti. All the p-values were more than the effective alpha values for all OTUs in stool samples. Therefore, there was no observed significant enrichment of any OTU in the patient samples from stool samples. Conclusion: In healthy volunteers of multi ethnicity, due to the influence of the various factors, correlation betwwen the prakriti and the gut microbiome was not seen. The correlation can be established by controlling the factors that would influence the gut microbiome.
{"title":"Exploring the Correlation between Body Constitution of an Individual as Per Ayurveda and Gut Microbiome in Healthy, Multi Ethnic Urban Population in Bangalore, India","authors":"Shalini Tv, Gangadharan Gg, Sriranjini Jaideep, Asn Seshasayee, A. Pandit","doi":"10.4303/2471-9455/8/236068","DOIUrl":"https://doi.org/10.4303/2471-9455/8/236068","url":null,"abstract":"Prakriti (body –mind constitution of an individual) is a conventional, customized and unique the understanding of which is essential for the personalized medicine described in Ayurveda, Indian System of Medicine. Based on the Doshas( functional, bio humoral unit in the body) individuals are categorized into three major prakriti- Vata, Pitta and Kapha. The human gut micro biome hosts plenty of highly diverse and metabolically active microorganisms, mainly dominated by the bacteria, which are known to influence the physiology of an individual. Few researches have shown the correlation between the prakriti and the biochemical parameters. In this study, an attempt was made to explore any correlation between the prakriti (phenotype of an individual) with the Genetic makeup of the gut micro biome in healthy individuals. Materials and methods: 270 multi ethnic, healthy volunteers of both the sex with the age group between 18 to 40 years, with no history of antibiotics in last 6 months were recruited into three groups of Vata, Pitta and Kapha. The Prakriti of the individual was determined using Ayusoft, a software designed by CDAC, Pune, India. The volunteers were subjected to initial screening for the assessment of their height, weight, Body Mass Index, Vital signs and Blood investigations to ensure they are healthy. The stool sample of the recruited volunteers were collected as per the standard operating procedure developed and the bacterial DNA was isolated using Qiagen kits. The extracted DNA was subjected to 16s rRNA sequencing using the Illumina kits. The sequencing libraries targeting the variable V3 and V4 regions of the 16s rRNA gene. Paired sequencing was done on MiSeq system and data were analyzed using the CLC Genomics workbench 11. Results: The 16s rRNA sequencing of V3 and V4 region showed diverse pattern in both the oral and stool microbial DNA. The study did not reveal any specific pattern of bacterial flora amongst the prakriti. All the p-values were more than the effective alpha values for all OTUs in stool samples. Therefore, there was no observed significant enrichment of any OTU in the patient samples from stool samples. Conclusion: In healthy volunteers of multi ethnicity, due to the influence of the various factors, correlation betwwen the prakriti and the gut microbiome was not seen. The correlation can be established by controlling the factors that would influence the gut microbiome.","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81749790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-28DOI: 10.17011/conference/eccb2018/108171
J. Baker
{"title":"Lessons Learnt from Industry: Achieving Net Gain Outcomes for Biodiversity","authors":"J. Baker","doi":"10.17011/conference/eccb2018/108171","DOIUrl":"https://doi.org/10.17011/conference/eccb2018/108171","url":null,"abstract":"","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86526184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Halophilic bacteria are organisms which thrive in salt-rich environments, such as salt lakes, solar salterns and salt mines which contain large populations of these organisms. In biotechnology, such salt-tolerant bacteria are widely used for the production of valuable enzymes, and more than a thousand years ago humans began using salt to cure and thereby preserve perishable foods and other materials, such as hides; halophiles can be detrimental to the preservation of salt brine cured hides. The aim of this review is to provide an overview of the taxonomy of these organisms including novel isolates from rock salt, and also to discuss their current and future biotechnological and environmental uses.
{"title":"Halophilic Bacterium: A Review of New Studies","authors":"Bassam Oudh Al Johny","doi":"10.13005/bbra/1874","DOIUrl":"https://doi.org/10.13005/bbra/1874","url":null,"abstract":"Halophilic bacteria are organisms which thrive in salt-rich environments, such as salt lakes, solar salterns and salt mines which contain large populations of these organisms. In biotechnology, such salt-tolerant bacteria are widely used for the production of valuable enzymes, and more than a thousand years ago humans began using salt to cure and thereby preserve perishable foods and other materials, such as hides; halophiles can be detrimental to the preservation of salt brine cured hides. The aim of this review is to provide an overview of the taxonomy of these organisms including novel isolates from rock salt, and also to discuss their current and future biotechnological and environmental uses.","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76952841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natural products from herb have been used in different aspects of life as a result of their various biological activities. Generally, plant growth and production of secondary compounds largely depend on environmental conditions. To better understand this correlation, study on biological activity and soil parameter is necessary. This research aims to study the soil parameters which affect the efficiency of the antioxidant activity of essential oils extracted from the Zingiber zerumbet in three areas of Thailand, including Min Buri district, Bangkok province; Muang district, Chiang Mai province and Kaeng Sanam Nang district, Nakhon Ratchasima province. The soil samples in each area were collected and analyzed in the laboratory. The essential oil of Z. zerumbet in each province was extracted and tested for antioxidant activity by hydrodistillation method and DPPH (2,2-diphenyl-1-picrylhydrazyl radical) assay, respectively. The results showed that, the soil parameters such as pH, nitrogen, potassium and phosphorus elements and exchange of cations of soil specimen from Nakhon Ratchasima province were the highest (P<0.05) (6.10 ±0.03, 0.15 ± 0.04 percent of total nitrogen, 16.67 ± 0.46 mg/L, 3.35 ± 0.65 mg/kg and 12.87 ± 0.11 cmol/kg, respectively). In addition, IC50 (Inhibition Concentrtion of antioxidant at 50%) of Z. zerumbet essential oil collected from Nakhon Ratchasima showed the highest value (P<0.05) (1,400 µg/mL). In conclusion, the soil parameters are once important factor for the efficiency of essential oils extract from Z. zerumbet.
{"title":"The Effects of Soil Parameters on Efficiency of Essential Oil from Zingiber zerumbet (L.) Smith in Thailand","authors":"Worakrit Worananthakij, Kamonchanok Doungtadum, Nattagan Mingkwan, Supatsorn Chupong","doi":"10.5281/ZENODO.1125537","DOIUrl":"https://doi.org/10.5281/ZENODO.1125537","url":null,"abstract":"Natural products from herb have been used in different aspects of life as a result of their various biological activities. Generally, plant growth and production of secondary compounds largely depend on environmental conditions. To better understand this correlation, study on biological activity and soil parameter is necessary. This research aims to study the soil parameters which affect the efficiency of the antioxidant activity of essential oils extracted from the Zingiber zerumbet in three areas of Thailand, including Min Buri district, Bangkok province; Muang district, Chiang Mai province and Kaeng Sanam Nang district, Nakhon Ratchasima province. The soil samples in each area were collected and analyzed in the laboratory. The essential oil of Z. zerumbet in each province was extracted and tested for antioxidant activity by hydrodistillation method and DPPH (2,2-diphenyl-1-picrylhydrazyl radical) assay, respectively. The results showed that, the soil parameters such as pH, nitrogen, potassium and phosphorus elements and exchange of cations of soil specimen from Nakhon Ratchasima province were the highest (P<0.05) (6.10 ±0.03, 0.15 ± 0.04 percent of total nitrogen, 16.67 ± 0.46 mg/L, 3.35 ± 0.65 mg/kg and 12.87 ± 0.11 cmol/kg, respectively). In addition, IC50 (Inhibition Concentrtion of antioxidant at 50%) of Z. zerumbet essential oil collected from Nakhon Ratchasima showed the highest value (P<0.05) (1,400 µg/mL). In conclusion, the soil parameters are once important factor for the efficiency of essential oils extract from Z. zerumbet.","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"50 1","pages":"425-429"},"PeriodicalIF":0.0,"publicationDate":"2016-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84548267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-22DOI: 10.1158/2159-8290.cd-rw2012-044
Noora Al Muftah, Reda Rawi, Richard B. Thompson, H. Bensmail
{"title":"Cell Line Screens Identify Biomarkers of Drug Sensitivity in GLIOMA Cancer","authors":"Noora Al Muftah, Reda Rawi, Richard B. Thompson, H. Bensmail","doi":"10.1158/2159-8290.cd-rw2012-044","DOIUrl":"https://doi.org/10.1158/2159-8290.cd-rw2012-044","url":null,"abstract":"","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89290404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-13DOI: 10.21275/v4i11.nov151774
N. Khatik, R. Joshi
An in vitro plant regeneration system was developed via direct somatic embryogenesis from different seedling explants of an important medicinal plant Murraya koenigii (L.) Spreng Cotyledons (COT), Hypocotyl (HYP) (10 to 15 mm) and Root (RT) segments (10 to 20 mm) were excised from 60 days old seedling as explants. The somatic embryos induction was achieved on Murashige and Skoog (MS) basal medium augmented with different concentrations of 6-benzyleaminopurine (BAP) 1.33 to 8.40 μM and thidiazuron (TDZ) 1.08 to 9.82 μM. The globular embryos originated from cut ends and entire surface of the root, hypocotyl explants and margins of cotyledons within 30-40 days. The percentage of somatic embryos induction per explant was significantly higher in HYP explants (94.21±5.77%) in the MS basal medium supplemented with 6.20 μM BAP and 8.64 μM TDZ. The highest rate of conversion of torpedo, heart and cotyledonary stages from globular stage was obtained in MS medium supplemented with 8.64 μM TDZ. The matured somatic embryos were transferred to the MS basal medium without Plant Growth Regulators (PGRs). Highest 88% of the matured embryos were germinated on transfer to 1⁄2 MS basal medium without PGR, where they grew for a further 3-4 weeks. Out of seventy six hardened plants seventy (92%) plantlets were found healthy under field conditions.
{"title":"Regeneration of Plantlets via Direct Somatic Embryogenesis from Different Explants of Murraya koenigii","authors":"N. Khatik, R. Joshi","doi":"10.21275/v4i11.nov151774","DOIUrl":"https://doi.org/10.21275/v4i11.nov151774","url":null,"abstract":"An in vitro plant regeneration system was developed via direct somatic embryogenesis from different seedling explants of an important medicinal plant Murraya koenigii (L.) Spreng Cotyledons (COT), Hypocotyl (HYP) (10 to 15 mm) and Root (RT) segments (10 to 20 mm) were excised from 60 days old seedling as explants. The somatic embryos induction was achieved on Murashige and Skoog (MS) basal medium augmented with different concentrations of 6-benzyleaminopurine (BAP) 1.33 to 8.40 μM and thidiazuron (TDZ) 1.08 to 9.82 μM. The globular embryos originated from cut ends and entire surface of the root, hypocotyl explants and margins of cotyledons within 30-40 days. The percentage of somatic embryos induction per explant was significantly higher in HYP explants (94.21±5.77%) in the MS basal medium supplemented with 6.20 μM BAP and 8.64 μM TDZ. The highest rate of conversion of torpedo, heart and cotyledonary stages from globular stage was obtained in MS medium supplemented with 8.64 μM TDZ. The matured somatic embryos were transferred to the MS basal medium without Plant Growth Regulators (PGRs). Highest 88% of the matured embryos were germinated on transfer to 1⁄2 MS basal medium without PGR, where they grew for a further 3-4 weeks. Out of seventy six hardened plants seventy (92%) plantlets were found healthy under field conditions.","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89582411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryma Amamra, M. Djebar, N. Grara, Ouissem Moumeni, H. Otmani, Amel Alayat, H. Berrebbah
Synthetic Pyrethroids are considered to be safe over other insecticides; however, data indicate that their use may pose risk to environmental biota, especially, aquatic organisms. Therefore, the aim of the present study was to investigate the effect of cypermethrin, a widely used insecticide and one of the most common contaminants in freshwater aquatic system on the oxidative stress biomarkers of the freshwater ciliate Paramecium tetraurelia. After the treatment of paramecium cells with increasing concentrations of cypermethrin (0.05, 0.5,1, 2�g/l), we followed up the growth kinetics, generation time and the response percentage. Also, we studied the variation in biomarkers of stress such as: Proteins, GSH content, GST and CAT activities. Our results showed a significant decrease in the proliferation of cell, we denote a difference of
{"title":"Cypermethrin-Induces Oxidative Stress to the Freshwater Ciliate Model: Paramecium tetraurelia","authors":"Ryma Amamra, M. Djebar, N. Grara, Ouissem Moumeni, H. Otmani, Amel Alayat, H. Berrebbah","doi":"10.9734/ARRB/2015/10852","DOIUrl":"https://doi.org/10.9734/ARRB/2015/10852","url":null,"abstract":"Synthetic Pyrethroids are considered to be safe over other insecticides; however, data indicate that their use may pose risk to environmental biota, especially, aquatic organisms. Therefore, the aim of the present study was to investigate the effect of cypermethrin, a widely used insecticide and one of the most common contaminants in freshwater aquatic system on the oxidative stress biomarkers of the freshwater ciliate Paramecium tetraurelia. After the treatment of paramecium cells with increasing concentrations of cypermethrin (0.05, 0.5,1, 2�g/l), we followed up the growth kinetics, generation time and the response percentage. Also, we studied the variation in biomarkers of stress such as: Proteins, GSH content, GST and CAT activities. Our results showed a significant decrease in the proliferation of cell, we denote a difference of","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73271098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. A. Mutairi, M. Mansor, M. El-Bana, S. Al‐Rowaily, A. Mansor
Khalid Al Mutairi1, Mashhor Mansor1, Magdy El-Bana2,3,*, Saud L. Al-Rowaily2 and Asyraf Mansor1 1School of Biological Sciences, Universiti Sains Malaysia, Penang, 2Department of Plant Production, College of Agricultural & Food Sciences, King Saud University, Riyadh, 3Department of Biological Sciences, Faculty of Education at El-Arish, Suez Canal University, El-Arish, 1Malaysia 2Saudi Arabia 3Egypt
Khalid Al Mutairi1, Mashhor Mansor1, Magdy El-Bana2,3，*， Saud L. Al- rowaily2和Asyraf Mansor1 1马来西亚圣城大学生物科学学院，2利雅得沙特国王大学农业与食品科学学院植物生产系，3苏伊士运河大学El-Arish教育学院生物科学系，El-Arish, 1马来西亚2沙特阿拉伯3埃及
{"title":"Influences of Island Characteristics on Plant Community Structure of Farasan Archipelago, Saudi Arabia: Island Biogeography and Nested Pattern","authors":"K. A. Mutairi, M. Mansor, M. El-Bana, S. Al‐Rowaily, A. Mansor","doi":"10.5772/33704","DOIUrl":"https://doi.org/10.5772/33704","url":null,"abstract":"Khalid Al Mutairi1, Mashhor Mansor1, Magdy El-Bana2,3,*, Saud L. Al-Rowaily2 and Asyraf Mansor1 1School of Biological Sciences, Universiti Sains Malaysia, Penang, 2Department of Plant Production, College of Agricultural & Food Sciences, King Saud University, Riyadh, 3Department of Biological Sciences, Faculty of Education at El-Arish, Suez Canal University, El-Arish, 1Malaysia 2Saudi Arabia 3Egypt","PeriodicalId":23973,"journal":{"name":"World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84610918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：