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Role of miR-128/216a Regulating Isl1 Expression during Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Insulin-Producing Cells miR-128/216a在人脐带间充质干细胞向胰岛素产生细胞分化过程中调节Isl1表达的作用
Q3 Multidisciplinary Pub Date : 2023-04-01 DOI: 10.1051/wujns/2023282177
Tingting Mu, Tao Wang, Zhenyu Gao, Xin Pan, Yingxue Liu
Islet-1 (Isl1), a LIM homeodomain protein, is expressed in the embryonic pancreatic epithelium. As a key transcription factor, Isl1 can not only regulate insulin gene expression in normal glucose condition but also maintain β-cell function and impact pancreatic β-cell target genes. Some experiments have suggested that MicroRNA (miRNA) can play a critical role during the induction of insulin-producing cells (IPCs). However, it is unclear whether miRNA may regulate Isl1 expression during differentiation of human umbilical cord mesenchymal stem cells (HUMSCs) into IPCs. In this investigation, we induced HUMSCs into IPCs with a modified two-step protocol, activin A, retinoic acid (step1) and conophylline, nicotinamide (step2). To find the miRNA regulating Isl1 expression, we respectively used TargetScan, miRDB and RNAhybrid to predict and got the result, miR-128 and miR-216a. The miRNAs can inhibit Isl1 expression by dual luciferase assay. The results of real-time Polymerase Chain Reaction (PCR) showed that Isl1 expression level was almost reciprocal to that of miR-128 and miR-216a during differentiation of HUMSCs into IPCs. Furthermore, over-expression of miR-128 or miR-216a down-regulated expression levels of Isl1 and MafA. Therefore, miR-128 or miR-216a may regulate expression of islet-specific transcription factors to control differentiation of HUMSCs into IPCs.
胰岛1(Isl1)是一种LIM同源结构域蛋白,在胚胎胰腺上皮中表达。作为一种关键的转录因子,Isl1不仅可以在正常葡萄糖条件下调节胰岛素基因的表达,还可以维持β细胞功能并影响胰腺β细胞靶基因。一些实验表明,微小RNA(miRNA)可以在诱导胰岛素产生细胞(IPC)过程中发挥关键作用。然而,尚不清楚miRNA是否可以在人脐带间充质干细胞(HUMSCs)分化为IPCs的过程中调节Isl1的表达。在本研究中,我们采用改良的两步方案,激活素a、视黄酸(第1步)和钩藤碱、烟酰胺(第2步),将HUMSCs诱导为IPCs。为了寻找调节Isl1表达的miRNA,我们分别使用TargetScan、miRDB和RNAhybrid进行预测,得到了miR-128和miR-216a的结果。通过双荧光素酶测定,miRNA可以抑制Isl1的表达。实时聚合酶链反应(PCR)的结果表明,在HUMSCs分化为IPCs的过程中,Isl1的表达水平几乎与miR-128和miR-216a的表达水平相反。此外,miR-128或miR-216a的过度表达下调了Isl1和MafA的表达水平。因此,miR-128或miR-216a可以调节胰岛特异性转录因子的表达,以控制HUMSCs分化为IPCs。
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引用次数: 0
Optimal Investment of Defined Contribution Pension Based on Self-Protection and Minimum Security 基于自我保护和最低安全性的固定缴款养老金最优投资
Q3 Multidisciplinary Pub Date : 2023-04-01 DOI: 10.1051/wujns/2023282129
Lan Wang, C. Wang, Juan Xue
This paper mainly studies the optimal investment problem of defined contribution (DC) pension under the self-protection and minimum security. First, we apply [see formula in PDF] theorem to obtain the differential equation of the real stock price after discounting inflation. Then, under the constraint of external guarantee of DC pension terminal wealth, self-protection is introduced to study the maximization of the expected utility of terminal wealth at retirement time and any time before retirement. The explicit solution of the optimal investment strategy of DC pension at retirement time and any time before retirement should be derived by martingale method. Finally, the influence of self-protection on the optimal investment strategy of DC pension is numerically analyzed.
本文主要研究在自我保护和最低安全条件下的固定缴款养老金的最优投资问题。首先,我们应用[见PDF中的公式]定理来获得扣除通货膨胀后的实际股价的微分方程。然后,在DC养老金终端财富外部保障的约束下,引入自我保护来研究退休时和退休前任何时间终端财富的预期效用最大化问题。DC养老金在退休时间和退休前任何时间的最优投资策略的显式解都应该用鞅方法导出。最后,数值分析了自我保护对DC养老金最优投资策略的影响。
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引用次数: 0
Periocular Biometric Recognition for Masked Faces 蒙面人脸眼周生物识别
Q3 Multidisciplinary Pub Date : 2023-04-01 DOI: 10.1051/wujns/2023282141
Qiaoyue Huang, Chaoying Tang, Tianshu Zhang
Since the outbreak of Coronavirus Disease 2019 (COVID-19), people are recommended to wear facial masks to limit the spread of the virus. Under the circumstances, traditional face recognition technologies cannot achieve satisfactory results. In this paper, we propose a face recognition algorithm that combines the traditional features and deep features of masked faces. For traditional features, we extract Local Binary Pattern (LBP), Scale-Invariant Feature Transform (SIFT) and Histogram of Oriented Gradient (HOG) features from the periocular region, and use the Support Vector Machines (SVM) classifier to perform personal identification. We also propose an improved Convolutional Neural Network (CNN) model Angular Visual Geometry Group Network (A-VGG) to learn deep features. Then we use the decision-level fusion to combine the four features. Comprehensive experiments were carried out on databases of real masked faces and simulated masked faces, including frontal and side faces taken at different angles. Images with motion blur were also tested to evaluate the robustness of the algorithm. Besides, the experiment of matching a masked face with the corresponding full face is accomplished. The experimental results show that the proposed algorithm has state-of-the-art performance in masked face recognition, and the periocular region has rich biological features and high discrimination.
自2019冠状病毒病(新冠肺炎)爆发以来,建议人们戴口罩以限制病毒的传播。在这种情况下,传统的人脸识别技术无法取得令人满意的效果。在本文中,我们提出了一种人脸识别算法,该算法结合了传统特征和掩蔽人脸的深层特征。对于传统特征,我们从眼周区域提取局部二元模式(LBP)、尺度不变特征变换(SIFT)和面向梯度直方图(HOG)特征,并使用支持向量机(SVM)分类器进行个人识别。我们还提出了一种改进的卷积神经网络(CNN)模型角视觉几何群网络(A-VGG)来学习深层特征。然后,我们使用决策级融合来组合这四个特征。在真实人脸和模拟人脸的数据库上进行了综合实验,包括不同角度拍摄的正面和侧面人脸。还对具有运动模糊的图像进行了测试,以评估算法的鲁棒性。此外,还完成了将蒙面与相应的全脸进行匹配的实验。实验结果表明,该算法在蒙面人脸识别中具有最先进的性能,眼周区域具有丰富的生物特征和较高的识别率。
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引用次数: 0
Short-Term Wind Power Prediction Method Based on Combination of Meteorological Features and CatBoost 基于气象特征与CatBoost结合的短期风电预测方法
Q3 Multidisciplinary Pub Date : 2023-04-01 DOI: 10.1051/wujns/2023282169
Xingyu Mou, Hui Chen, Xinjing Zhang, Xin Xu, Qingbo Yu, Yun Li
As one of the hot topics in the field of new energy, short-term wind power prediction research should pay attention to the impact of meteorological characteristics on wind power while improving the prediction accuracy. Therefore, a short-term wind power prediction method based on the combination of meteorological features and CatBoost is presented. Firstly, morgan-stone algebras and sure independence screening(MS-SIS) method is designed to filter the meteorological features, and the influence of the meteorological features on the wind power is explored. Then, a sort enhancement algorithm is designed to increase the accuracy and calculation efficiency of the method and reduce the prediction risk of a single element. Finally, a prediction method based on CatBoost network is constructed to further realize short-term wind power prediction. The National Renewable Energy Laboratory (NREL) dataset is used for experimental analysis. The results show that the short-term wind power prediction method based on the combination of meteorological features and CatBoost not only improve the prediction accuracy of short-term wind power, but also have higher calculation efficiency.
风电短期预测研究作为新能源领域的热点之一,在提高预测精度的同时,应关注气象特征对风电的影响。为此,提出了一种结合气象特征和CatBoost的短期风电功率预测方法。首先,设计了morgan-stone代数和确定独立筛选(MS-SIS)方法对气象特征进行过滤,并探讨了气象特征对风电的影响;然后,设计了排序增强算法,提高了方法的精度和计算效率,降低了单个元素的预测风险。最后,构建了基于CatBoost网络的风电短期预测方法,进一步实现了风电短期预测。实验分析使用国家可再生能源实验室(NREL)数据集。结果表明,基于气象特征与CatBoost相结合的短期风电预测方法不仅提高了短期风电的预测精度,而且具有较高的计算效率。
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引用次数: 0
Adversarial Example Generation Method Based on Sensitive Features 基于敏感特征的对抗样例生成方法
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281035
Zerui Wen, Zhidong Shen, Hui Sun, Baiwen Qi
As deep learning models have made remarkable strides in numerous fields, a variety of adversarial attack methods have emerged to interfere with deep learning models. Adversarial examples apply a minute perturbation to the original image, which is inconceivable to the human but produces a massive error in the deep learning model. Existing attack methods have achieved good results when the network structure is known. However, in the case of unknown network structures, the effectiveness of the attacks still needs to be improved. Therefore, transfer-based attacks are now very popular because of their convenience and practicality, allowing adversarial samples generated on known models to be used in attacks on unknown models. In this paper, we extract sensitive features by Grad-CAM and propose two single-step attacks methods and a multi-step attack method to corrupt sensitive features. In two single-step attacks, one corrupts the features extracted from a single model and the other corrupts the features extracted from multiple models. In multi-step attack, our method improves the existing attack method, thus enhancing the adversarial sample transferability to achieve better results on unknown models. Our method is also validated on CIFAR-10 and MINST, and achieves a 1%-3% improvement in transferability.
随着深度学习模型在许多领域取得显著进展,各种对抗性攻击方法已经出现,以干扰深度学习模型。对抗性示例对原始图像施加微小的扰动,这对人类来说是不可想象的,但会在深度学习模型中产生巨大的误差。现有的攻击方法在网络结构已知的情况下取得了很好的效果。但是,在网络结构未知的情况下,攻击的有效性还有待提高。因此,基于转移的攻击由于其便利性和实用性,现在非常流行,它允许在已知模型上生成的对抗性样本用于对未知模型的攻击。本文利用Grad-CAM提取敏感特征,提出了破坏敏感特征的两种单步攻击方法和多步攻击方法。在两个单步攻击中,一个破坏从单个模型中提取的特征,另一个破坏从多个模型中提取的特征。在多步攻击中,我们的方法改进了现有的攻击方法,从而提高了对抗样本的可转移性,在未知模型上取得了更好的结果。我们的方法也在CIFAR-10和MINST上进行了验证,可移植性提高了1%-3%。
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引用次数: 0
Warming Stimulated Soil Respiration in a Subalpine Meadow in North China 变暖对华北亚高山草甸土壤呼吸的影响
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281077
S. Luo, Jianhua Zhang, Hufang Zhang, Qin Zheng, Yongping Gao, Meihong Li
In order to explore the response of soil respiration in grassland to global warming, we carried out a warming experiment with open top chambers (OTCs) in the subalpine meadow, Mount Wutai in north China. Our results showed in the subalpine meadow across 2 500-2 700 m above the sea level (ASL), with OTCs, soil respiration increased by 2.00 μmol·m-2·s-1 as soil temperature increased by 1.25 ℃ on average. Warming decreased soil moisture over the experiment periods except in October 2019 when snow melted in OTCs. Warming effect on soil respiration peaked at 178.31% in October 2019. In control and warming treatment, based on exponential regression equations, soil temperature alone accounted for 85.3% and 61.2% of soil respiration variation, respectively. In control treatment soil moisture alone explained 23.2% of soil respiration variation based on the power regression equation while in warming treatment they were not significantly correlated with each other. The response of soil respiration to warming relied on altitudes as well as the time of the year, but was not inhibited by soil moisture, labile carbon pool, and available nitrogen. We concluded soil temperature was the main factor influencing soil respiration, and global warming would stimulate soil respiration in the subalpine meadows of Mount Wutai in the future. Our analysis provided new data on characteristics and mechanisms of the response of soil respiration to warming, and helped to further understand the relationship between carbon cycle and climate change.
为了探讨草原土壤呼吸对全球变暖的响应,我们在中国北方五台山亚高山草甸进行了开放顶室增温实验。研究结果表明,在海拔2500~2700米的亚高山草甸中,OTCs的土壤呼吸平均随土壤温度的升高而增加2.00μmol·m-2·s-1。除2019年10月OTCs中的雪融化外,在实验期间,变暖降低了土壤湿度。变暖对土壤呼吸的影响在2019年10月达到178.31%的峰值。在控制和增温处理中,基于指数回归方程,单独土壤温度分别占土壤呼吸变化的85.3%和61.2%。在对照处理中,土壤水分单独解释了23.2%的土壤呼吸变化,而在增温处理中,它们之间没有显著的相关性。土壤呼吸对变暖的反应取决于海拔和一年中的时间,但不受土壤湿度、不稳定碳库和有效氮的抑制。土壤温度是影响土壤呼吸的主要因素,未来全球变暖将刺激五台山亚高山草甸的土壤呼吸。我们的分析为土壤呼吸对变暖的响应特征和机制提供了新的数据,并有助于进一步了解碳循环与气候变化之间的关系。
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引用次数: 0
An Image Denoising Model via the Reconciliation of the Sparsity and Low-Rankness of the Dictionary Domain Coefficients 基于字典域系数稀疏性和低秩性的图像去噪模型
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281061
Yifan Yang, Tao Zhang, Di Wu, Yu Zhao
Sparse coding has achieved great success in various image restoration tasks. However, if the sparse representation coefficients of the structure (low-frequency information) and texture (high-frequency information) components of the image are under the same penalty constraint, the restoration effect may not be ideal. In this paper, an image denoising model combining mixed norm and weighted nuclear norm as regularization terms is proposed. The proposed model simultaneously exploits the group sparsity of the high frequency and low-rankness of the low frequency in dictionary-domain. The mixed norm is used to constrain the high frequency part and the weighted nuclear norm is used to constrain the low frequency part. In order to make the proposed model easy to solve under the framework of alternative direction multiplier method (ADMM), iterative shrinkage threshold method and weighted nuclear norm minimization method are used to solve the two sub-problems. The validity of the model is verified experimentally through comparison with some state-of-the-art methods.
稀疏编码在各种图像恢复任务中取得了巨大成功。然而,如果图像的结构(低频信息)和纹理(高频信息)分量的稀疏表示系数处于相同的惩罚约束下,则恢复效果可能不理想。本文提出了一种将混合范数和加权核范数作为正则项的图像去噪模型。该模型同时利用了字典域中高频的群稀疏性和低频的低秩性。混合范数用于约束高频部分,加权核范数用于约束低频部分。为了使所提出的模型在交替方向乘法器方法(ADMM)的框架下易于求解,使用迭代收缩阈值方法和加权核范数最小化方法来求解这两个子问题。通过与一些最先进的方法的比较,实验验证了该模型的有效性。
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引用次数: 0
Group Sparsity Residual Constraint Image Denoising Model with 𝒍1/𝒍2 Regularization
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281053
Di Wu, Zhang Tao, Xutao Mo
Group sparse residual constraint with non-local priors (GSRC) has achieved great success in image restoration producing state-of-the-art performance. In the GSRC model, the [see formula in PDF] norm minimization is employed to reduce the group sparse residual. In recent years, non-convex regularization terms have been widely used in image denoising problems, which have achieved better results in denoising than convex regularization terms. In this paper, we use the ratio of the [see formula in PDF] and [see formula in PDF] norm instead of the [see formula in PDF] norm to propose a new image denoising model, i.e., a group sparse residual constraint model with [see formula in PDF] minimization (GSRC-[see formula in PDF]). Due to the computational difficulties arisen from the non-convexity and non-linearity, we focus on a constrained optimization problem that can be solved by alternative direction method of multipliers (ADMM). Experimental results of image denoising show that the pro-posed model outperforms several state-of-the-art image denoising methods both visually and quantitatively.
具有非局部先验的群稀疏残差约束(GSRC)在图像恢复中取得了巨大成功,产生了最先进的性能。在GSRC模型中,使用[参见PDF中的公式]范数最小化来减少组稀疏残差。近年来,非凸正则化项被广泛应用于图像去噪问题,在去噪方面取得了比凸正则化项更好的效果。在本文中,我们使用[Seee formula In PDF]和[Seee formula In PDV]范数的比率来代替[Seee公式In PDF]范数,提出了一种新的图像去噪模型,即具有[Seee ormula In PDP]最小化的组稀疏残差约束模型(GSRC-[Seee formula In PDFC])。由于非凸性和非线性带来的计算困难,我们将重点研究一个可以通过乘法器的交替方向法(ADMM)求解的约束优化问题。图像去噪实验结果表明,该模型在视觉和定量上都优于几种最先进的图像去噪方法。
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引用次数: 0
Mechanical Properties of Laser Welded Joint of Copper and Steel Dissimilar Metals 铜与钢异种金属激光焊接接头的力学性能
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281068
Yiwen Ren, Junhui Tao, Jie Li, Xinqi Chen, Lin Zhang, Chuanhui Wang, Haiqin Jin, Hongyan Qi, Zhu A. Wang, X. Xie, J. Pan
In this research project, copper and stainless steel were connected by two laser welding methods: straight seam welding and swing welding. Then, electronic tensile test machine, X-ray diffractometer, scanning electron microscope and metallographic microscope were used to analyze the tensile properties, macroscopic and microscopic structure morphology and phase of the welded joint. Based on the experimental results, we determined that the strength of the straight seam welded joint was higher. Because of the intermetallic compound near the weld in the swing welding process, it leads to stress concentration, crack cracking and strength reduction. In addition, the oscillating laser beam also leads to the disorderly direction of columnar crystal and coarse structure, which makes the joint strength decrease.
在本研究项目中,采用直缝焊和摆焊两种激光焊接方法连接铜和不锈钢。然后利用电子拉伸试验机、x射线衍射仪、扫描电镜和金相显微镜对焊接接头的拉伸性能、宏观和微观组织形貌及物相进行分析。根据试验结果,确定了直缝焊接接头的强度较高。在摆焊过程中,由于焊缝附近存在金属间化合物,导致应力集中,裂纹开裂,强度降低。此外,激光束的振荡还导致柱状晶体方向无序,结构粗糙,使接头强度降低。
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引用次数: 0
On Deep Holes of Projective Reed-Solomon Codes over Finite Fields with Even Characteristic 偶数特征有限域上投影Reed-Solomon码的深孔
Q3 Multidisciplinary Pub Date : 2023-02-01 DOI: 10.1051/wujns/2023281015
Xiaofang Xu
Projective Reed-Solomon code is an important class of maximal distance separable codes in reliable communication and deep holes play important roles in its decoding. In this paper, we obtain two classes of deep holes of projective Reed-Solomon codes over finite fields with even characteristic. That is, let [see formula in PDF] be finite field with even characteristic, [see formula in PDF], and let [see formula in PDF] be the Lagrange interpolation polynomial of the first [see formula in PDF] components of the received vector [see formula in PDF]. Suppose that the [see formula in PDF]-th component of [see formula in PDF] is 0, and [see formula in PDF],[see formula in PDF] where [see formula in PDF], and [see formula in PDF] is a polynomial over [see formula in PDF] with degree no more than [see formula in PDF]. Then the received vector [see formula in PDF] is a deep hole of projective Reed-Solomon codes [see formula in PDF]. In fact, our result partially solved an open problem on deep holes of projective Reed-Solomon codes proposed by Wan in 2020.
投影Reed-Solomon码是可靠通信中一类重要的最大距离可分离码,深空在其译码中起着重要作用。本文在具有偶特征的有限域上,得到了投影Reed-Solomon码的两类深孔。也就是说,设[见PDF中的公式]是具有偶数特征的有限域,[见PDF的公式],并且设[见PDF]是接收向量的第一个[见PDV]分量的拉格朗日插值多项式[见PDP]的公式]。假设[见PDF中的公式]的第[见PDF的公式]分量为0,并且[见PDF里的公式],[见PDF里面的公式]其中[见PDF里边的公式]和[见PDF下面的公式]是[见PDF上面的公式]上的多项式,次数不超过[见PDF后面的公式]。那么接收到的向量[见PDF中的公式]是投影Reed-Solomon码的一个深孔[见PDF]。事实上,我们的结果部分解决了Wan在2020年提出的一个关于投影Reed-Solomon码的深孔的开放问题。
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引用次数: 0
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Wuhan University Journal of Natural Sciences
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