Background: It has been reported that transforming growth factor-beta (TGF-β) is elevated in the subsynovial connec tive tissues (SSCTs) of patients with idiopathic carpal tunnel syndrome (CTS) and TGF-β induced fibrosis of SSCTs. A TGF-β-mediated pathway may be a potential target for CTS therapy. Cycloxygenase-2 (COX-2) and nerve growth factor (NGF) are regulated by TGF-β and are associated with fi brosis in several tissues; however, regulation in SSCTs has not been fully clarified. Methods: Forty-three SSCTs were sampled from 35 pa tients undergoing carpal tunnel release (CTR) surgery. Cor relation between TGF-β mRNA and protein expression and COX-2 and NGF were evaluated using quantitative PCR (q-PCR) analysis of total RNA sampled from the SSCTs. To investigate regulation of COX-2 and NGF by TGF-β, cul tured SSCT cells were stimulated with TGF-β and COX-2 and NGE mRNA and protein expression were evaluated us ing q-PCR and western blotting. Results: TGF-β-, NGF-, and COX-2-positive cells were ob served in SSCTs. Expression of COX-2 and NGF positively correlated with TGF-β mRNA expression in the SSCTs of CTS patients (COX-2, r = 0.629, p < 0.001; NGF, r = 0.521, p < 0.001). The expression of COX-2 and NGF mRNA and protein increased significantly in SSCT cells following ex ogenous treatment with TGF-β compared to vehicle-treated cells (p < 0.05). Conclusions: TGF-β may regulate COX-2 and NGF in the SSCTs of CTS patients.
背景:据报道，特发性腕管综合征(CTS)患者滑膜下结缔组织(SSCTs)中转化生长因子-β (TGF-β)升高，并伴有TGF-β诱导的SSCTs纤维化。TGF-β介导的途径可能是CTS治疗的潜在靶点。环氧化酶-2 (COX-2)和神经生长因子(NGF)受TGF-β调控，并与多种组织的纤维化有关;然而，ssct的监管尚未得到充分澄清。方法:从35例接受腕管释放(CTR)手术的患者中抽取43例ssct。采用定量PCR (q-PCR)分析SSCTs总RNA，评估TGF-β mRNA和蛋白表达与COX-2和NGF之间的关系。为了研究TGF-β对COX-2和NGF的调节作用，我们用TGF-β和COX-2刺激培养的SSCT细胞，用q-PCR和western blotting检测NGE mRNA和蛋白的表达。结果:SSCTs中可见TGF-β-、NGF-、cox -2阳性细胞。CTS患者ssct中COX-2、NGF表达与TGF-β mRNA表达呈正相关(COX-2, r = 0.629, p < 0.001;NGF, r = 0.521, p < 0.001)。TGF-β外源处理SSCT细胞后，COX-2和NGF mRNA及蛋白的表达明显高于对照(p < 0.05)。结论:TGF-β可能调节CTS患者ssct中COX-2和NGF的表达。
{"title":"Transforming Growth Factor-Beta (TGF-Β) Regulates Nerve Growth Factor and Cyclooxygenase-2 Expression in Subsynovial Connective Tissue in Patients with Carpal Tunnel Syndrome","authors":"Nakawaki Mitsufumi, Uchida Kentaro, Onuma Kenji, Sukegawa Koji, Matsumoto Toshihide, Inoue Gen, Sekiguchi Hiroyuki, Takaso Masashi","doi":"10.23937/IAOS-2017/1710002","DOIUrl":"https://doi.org/10.23937/IAOS-2017/1710002","url":null,"abstract":"Background: It has been reported that transforming growth factor-beta (TGF-β) is elevated in the subsynovial connec tive tissues (SSCTs) of patients with idiopathic carpal tunnel syndrome (CTS) and TGF-β induced fibrosis of SSCTs. A TGF-β-mediated pathway may be a potential target for CTS therapy. Cycloxygenase-2 (COX-2) and nerve growth factor (NGF) are regulated by TGF-β and are associated with fi brosis in several tissues; however, regulation in SSCTs has not been fully clarified. Methods: Forty-three SSCTs were sampled from 35 pa tients undergoing carpal tunnel release (CTR) surgery. Cor relation between TGF-β mRNA and protein expression and COX-2 and NGF were evaluated using quantitative PCR (q-PCR) analysis of total RNA sampled from the SSCTs. To investigate regulation of COX-2 and NGF by TGF-β, cul tured SSCT cells were stimulated with TGF-β and COX-2 and NGE mRNA and protein expression were evaluated us ing q-PCR and western blotting. Results: TGF-β-, NGF-, and COX-2-positive cells were ob served in SSCTs. Expression of COX-2 and NGF positively correlated with TGF-β mRNA expression in the SSCTs of CTS patients (COX-2, r = 0.629, p < 0.001; NGF, r = 0.521, p < 0.001). The expression of COX-2 and NGF mRNA and protein increased significantly in SSCT cells following ex ogenous treatment with TGF-β compared to vehicle-treated cells (p < 0.05). Conclusions: TGF-β may regulate COX-2 and NGF in the SSCTs of CTS patients.","PeriodicalId":244060,"journal":{"name":"International Archives of Orthopedic Surgery","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116764995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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