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Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future最新文献

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Immobilization Biocatalysis 固定化生物催化
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引用次数: 0
Plant Nutrition and Phytohormones 植物营养与植物激素
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引用次数: 0
Plant Tissue Culture 植物组织培养
S. Uma, Lakshmi, Saraswathi, Akbar, Mustaffa
Plant regeneration through somatic embryogenesis from immature and mature zygotic embryos of Musa acuminata ssp. burmannica Abstract A simple and efficient protocol has been developed for in vitro regeneration of M. acuminata ssp. burmannica (AA) plants. Somatic embryos were produced when immature and mature zygotic embryo explants were cultured on Mura-shige and Skoog medium supplemented with plant growth regulators 2,4-dichlorophenoxyacetic acid; (2,4-D), picloram or benzyl adenine and indole acetic acid. In general, immature embryos responded better than mature embryos. Callus proliferation was highest in medium supplemented with 2,4-D (4.5 μM). Subsequent transfer of callus to fresh medium produced rapidly proliferating embryogenic calli. Embryo-genic calli were maintained in complete darkness for 15 d followed by cycles of 8 h dark and 16 h light, under white fluorescent lamps with a light intensity of 3,000 lm/m 2 and at temperature of 28±2°C. Regeneration of embryogenic calli into plantlets was higher for immature embryos (76.6%) than for mature embryos (50.6%). This plant regeneration protocol using mature or immature zygotic embryos, via somatic em-bryogenesis, has significant potential to improve germination efficiencies of hybrid progenies used in conventional breeding strategies. Furthermore, tests on seed storage showed that seed viability rapidly decline after harvesting and was negligible after 9 mo of storage. This indicates using freshly harvested seeds as explant material is necessary for maximizing the tissue culture response.
未成熟和成熟合子胚体细胞胚再生植株的研究。摘要建立了一种简单有效的体外再生方法。麻豆属植物。未成熟和成熟合子胚外植体分别在添加植物生长调节剂2,4-二氯苯氧乙酸的Mura-shige和Skoog培养基上培养,获得体胚;(2,4- d), picloram或苄基腺嘌呤和吲哚乙酸。一般来说,未成熟胚胎的反应比成熟胚胎好。在添加2,4- d (4.5 μM)的培养基中愈伤组织增殖能力最强。随后将愈伤组织转移到新鲜培养基中产生快速增殖的胚性愈伤组织。胚性愈伤组织在光照强度为3000 lm/ m2、温度为28±2℃的白色荧光灯下,在完全黑暗条件下培养15 d,然后进行8 h黑暗和16 h光照循环。未成熟胚的愈伤组织再生率(76.6%)高于成熟胚(50.6%)。这种利用成熟或未成熟的合子胚胎,通过体细胞胚-胚发生的植物再生方案,具有显著提高传统育种策略中使用的杂交后代萌发效率的潜力。此外,种子贮藏试验表明,种子活力在收获后迅速下降,贮藏9个月后可忽略不计。这表明使用新鲜收获的种子作为外植体材料是最大化组织培养反应的必要条件。
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引用次数: 0
Agricultural Biotechnology, Introduction, and History 农业生物技术,介绍和历史
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引用次数: 0
Plant Breeding and Seed Technology 植物育种与种子技术
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引用次数: 0
Use of Enzymes in Industry 酶在工业中的应用
P. Grunwald
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引用次数: 1
Enzymes in Non-Conventional Media 非常规培养基中的酶
P. Grunwald
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引用次数: 3
期刊
Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future
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