Yue Liu, Chang Liu, Chang Wang, Qian Zhang, Xingyuan Qu, Chen Liang, Lei Wang
Periodontitis is a chronic inflammatory disease in which dental plaque plays a key role. Hyperglycemia can promote inflammatory cells to secrete various inflammatory factors, causing the destruction of periodontal tissues and leading to the sustained development of periodontitis and prolonged and unhealed periodontal tissues. IL-1β is an important prophase inflammatory factor, which participates in the pathophysiological process of periodontitis. Interleukin 1 receptor antagonist (IL-1Ra), a natural inhibitor of IL-1, can also reduce the levels of other cytokines, including TNF-α and IL-6, and the balance between IL-1Ra and IL-1β is one of the primary factors affecting chronic periodontitis(CP). In this experiment, a temperature-sensitive hydrogel loaded with IL-1Ra was prepared and characterized, and its anti-inflammatory properties were evaluated. The hydrogel could be injected locally, and the results showed that it had good morphology, was nontoxic both in vivo and in vitro, and could realize sustained drug release. Histological evaluation and micro-computed tomography (micro-CT) showed that IL-1Ra-loaded thermosensitive hydrogel could effectively inhibit periodontal inflammation in diabetic rats, and played a significant role in relieving hyperglycemia. Therefore, IL-1Ra-loaded thermosensitive hydrogel may be an effective method to treat periodontitis with diabetes. Funding: This work was supported by grants from the Science and Technology Research Project of Education Department of Jilin Province(JJKH20211218KJ),"13th Five-Year plan" science and technology project of Education Department of Jilin Province(JJKH20201115KJ). Declaration of Interests: The authors report no declarations of interest. Ethics Approval Statement: This experiment was approved by the Ethics Committee of the Hospital of Stomatology, Jilin University,China, and all animal experiments followed the National Institute of Health (NIH) guidelines on laboratory animal care and use (ARRIVE guidelines).
{"title":"Treatment of Periodontal Inflammation in Diabetic Rats with Injectable IL-1Ra Thermosensitive Hydrogel","authors":"Yue Liu, Chang Liu, Chang Wang, Qian Zhang, Xingyuan Qu, Chen Liang, Lei Wang","doi":"10.2139/ssrn.3943648","DOIUrl":"https://doi.org/10.2139/ssrn.3943648","url":null,"abstract":"Periodontitis is a chronic inflammatory disease in which dental plaque plays a key role. Hyperglycemia can promote inflammatory cells to secrete various inflammatory factors, causing the destruction of periodontal tissues and leading to the sustained development of periodontitis and prolonged and unhealed periodontal tissues. IL-1β is an important prophase inflammatory factor, which participates in the pathophysiological process of periodontitis. Interleukin 1 receptor antagonist (IL-1Ra), a natural inhibitor of IL-1, can also reduce the levels of other cytokines, including TNF-α and IL-6, and the balance between IL-1Ra and IL-1β is one of the primary factors affecting chronic periodontitis(CP). In this experiment, a temperature-sensitive hydrogel loaded with IL-1Ra was prepared and characterized, and its anti-inflammatory properties were evaluated. The hydrogel could be injected locally, and the results showed that it had good morphology, was nontoxic both in vivo and in vitro, and could realize sustained drug release. Histological evaluation and micro-computed tomography (micro-CT) showed that IL-1Ra-loaded thermosensitive hydrogel could effectively inhibit periodontal inflammation in diabetic rats, and played a significant role in relieving hyperglycemia. Therefore, IL-1Ra-loaded thermosensitive hydrogel may be an effective method to treat periodontitis with diabetes. Funding: This work was supported by grants from the Science and Technology Research Project of Education Department of Jilin Province(JJKH20211218KJ),\"13th Five-Year plan\" science and technology project of Education Department of Jilin Province(JJKH20201115KJ). Declaration of Interests: The authors report no declarations of interest. Ethics Approval Statement: This experiment was approved by the Ethics Committee of the Hospital of Stomatology, Jilin University,China, and all animal experiments followed the National Institute of Health (NIH) guidelines on laboratory animal care and use (ARRIVE guidelines).","PeriodicalId":252984,"journal":{"name":"Allergy & Immunology eJournal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114340271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Since articular cartilage lacks blood vessels, nerves, and lymph tissue, its ability to repair itself is limited. Once damaged, it can lead to joint swelling and pain, accelerating the progression of osteoarthritis. Complete regeneration of hyaline cartilage with good mechanical properties remains an elusive goal, despite the many technologies available today. The inflammatory milieu created by cartilage damage is critical for chondrocyte death and hypertrophy, extracellular matrix breakdown, ectopic bone formation, and the progression of cartilage injury to osteoarthritis. In the inflammatory microenvironment, MSCs undergo aberrant differentiation, and chondrocytes begin to convert or dedifferentiate into cells with a fibroblast phenotype, resulting in fibrocartilage with poor mechanical qualities. All of these suggest that inflammatory problems may be a major stumbling block to cartilage repair. To produce a milieu conducive to cartilage regeneration, multi-dimensional management of the joint inflammatory microenvironment in place and time is required. Therefore, it is of great significance to elucidate the immune microenvironment of cartilage repair and regeneration after injury. This review provides a brief overview of:（1）the pathogenesis of cartilage injury（2）immune cells in cartilage injury and repair（3）effects of inflammatory cytokines on cartilage repair;（4）clinical strategies for the treatment of cartilage defects（5）strategies for targeted immunoregulation in cartilage repair and regeneration.
{"title":"The Immune Microenvironment in Cartilage Injury, Repair and Regeneration","authors":"Muzhe Li, Han Yin, Zineng Yan, Huiyun Li, Jiang Wu, Yue Wang, Fu Wei, Guangzhao Tian, Chao Ning, Haojiang Li, Cangjian Gao, Liwei Fu, Shuangpeng Jiang, Mingxue Chen, X. Sui, Shuyun Liu, Zhiwei Chen, Q. Guo","doi":"10.2139/ssrn.3906848","DOIUrl":"https://doi.org/10.2139/ssrn.3906848","url":null,"abstract":"Since articular cartilage lacks blood vessels, nerves, and lymph tissue, its ability to repair itself is limited. Once damaged, it can lead to joint swelling and pain, accelerating the progression of osteoarthritis. Complete regeneration of hyaline cartilage with good mechanical properties remains an elusive goal, despite the many technologies available today. The inflammatory milieu created by cartilage damage is critical for chondrocyte death and hypertrophy, extracellular matrix breakdown, ectopic bone formation, and the progression of cartilage injury to osteoarthritis. In the inflammatory microenvironment, MSCs undergo aberrant differentiation, and chondrocytes begin to convert or dedifferentiate into cells with a fibroblast phenotype, resulting in fibrocartilage with poor mechanical qualities. All of these suggest that inflammatory problems may be a major stumbling block to cartilage repair. To produce a milieu conducive to cartilage regeneration, multi-dimensional management of the joint inflammatory microenvironment in place and time is required. Therefore, it is of great significance to elucidate the immune microenvironment of cartilage repair and regeneration after injury. This review provides a brief overview of:（1）the pathogenesis of cartilage injury（2）immune cells in cartilage injury and repair（3）effects of inflammatory cytokines on cartilage repair;（4）clinical strategies for the treatment of cartilage defects（5）strategies for targeted immunoregulation in cartilage repair and regeneration.","PeriodicalId":252984,"journal":{"name":"Allergy & Immunology eJournal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116967264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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