Advances in Microscopic Imaging III最新文献

英文中文

Multimodal polarization-resolved/fluorescence optical scanning microscopy for chromatin organization imaging 多模态偏振分辨/荧光光学扫描显微镜用于染色质组织成像

Advances in Microscopic Imaging III

Pub Date : 2021-12-07 DOI: 10.1117/12.2615731

Ali Mohebi, A. Le Gratiet, F. Callegari, P. Bianchini, A. Diaspro

We demonstrate a combination of polarization-resolved with fluorescence optical scanning microscopy that offers polarizing based information along with molecular view of sample as a versatile tool for imaging the chromatin organization.

我们展示了偏振分辨与荧光光学扫描显微镜的结合，提供了基于偏振的信息以及样品的分子视图，作为成像染色质组织的多功能工具。

引用次数: 0

Sensorless adaptive optics for multimode optical fibre endo-microscopy 多模光纤内窥镜无传感器自适应光学

Advances in Microscopic Imaging III

Pub Date : 2021-12-07 DOI: 10.1117/12.2615656

Raphaël Turcotte, Carla C. Schmidt, N. Emptage, M. Booth

We demonstrate focus optimisation through multimode optical fibre using sensorless adaptive optics. The optimisation can correct for the three-dimensional shift occurring when re-positioning the fibre into the system for chronic brain imaging.

我们通过使用无传感器自适应光学的多模光纤演示焦点优化。这种优化可以纠正在将纤维重新定位到慢性脑成像系统时发生的三维位移。

引用次数: 0

Lensless microscopy through multiple determined masks 通过多个确定的掩模进行无透镜显微镜检查

Advances in Microscopic Imaging III

Pub Date : 2021-12-07 DOI: 10.1117/12.2615835

A. M. Khan, Iqra Bashir, Aqiba Hafeez, A. Hussain

In this paper, a transmission mode optical technique is required to achieve a super-resolved image. Basically, this technique is used for high resolution and for large field of view (FOV). The object is illuminated with deterministic mask patterns, and the object encoded information is transmitted to the sensor that is positioned at an optimum distance and records the images. A Spatial light modulator (SLM) is used as a mask and phase distribution of the SLM is changed ten times to captures ten different images. By using the angular spectrum process the captured images are backpropagated sequentially and reconstruct the super-resolved image. The use of multiple deterministic masks and Spatial Light Modulator (SLM) improved the quality of the reconstruction method. Such a technique aims to design an optical lens-less setup, working in transmission mode with portable and compact properties and to achieve a high resolution. This is much preferable to a conventional microscope in terms of resolution, the field of view, and low cost. To minimize the time of execution of the experiment, the coherent light source has been used tested. The super-resolution phase retrieval backward propagation algorithm is used to reconstruct the amplitude and phase image of the object from the captured intensity images. Advances in Microscopic Imaging III, edited by Emmanuel Beaurepaire, Adela Ben-Yakar, YongKeun Park, Proc. of SPIE-OSA Vol. 11922, 119221N · © 2021 OSA-SPIE CCC code: 1605-7422/21/$21 · doi: 10.1117/12.2615835 Proc. of SPIE-OSA Vol. 11922 119221N-1

在本文中，需要一种传输模式光学技术来实现超分辨图像。基本上，这种技术用于高分辨率和大视场(FOV)。物体被确定的掩模模式照射，物体编码信息被传输到位于最佳距离的传感器并记录图像。利用空间光调制器(SLM)作为掩模，对SLM的相位分布进行10次变换，获得10幅不同的图像。通过角谱处理，对捕获的图像进行顺序反向传播，重建出超分辨图像。使用多确定性掩模和空间光调制器(SLM)提高了重建方法的质量。该技术旨在设计一种光学无透镜装置，在传输模式下工作，具有便携和紧凑的特性，并实现高分辨率。就分辨率、视野和低成本而言，这比传统的显微镜要好得多。为了最大限度地减少实验的执行时间，实验中使用了相干光源。采用超分辨率相位恢复反向传播算法，从捕获的强度图像中重建目标的幅值和相位图像。《显微成像进展III》，由Emmanuel Beaurepaire, Adela Ben-Yakar, YongKeun Park编辑，prof . of spe - osa Vol. 11922, 119221N·©2021 OSA-SPIE CCC代码:1605-7422/21/$21·doi: 10.1117/12.2615835 prof . of spe - osa Vol. 11922, 119221N-1

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引用次数: 0

Chromatic dispersion-based wide-band, fiber-coupled, tunable light source for hyperspectral imaging 基于色散的宽带、光纤耦合、可调谐的高光谱成像光源

Advances in Microscopic Imaging III

Pub Date : 2021-12-07 DOI: 10.1117/12.2615809

Gargi Sharma, S. K. Naveed, Asha Parmar, Kanwarpal Singh

引用次数: 1

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全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

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